{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:24:56Z","timestamp":1789179896742,"version":"build-2803163510"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-02811-4","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T16:59:33Z","timestamp":1751389173000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of freezing and storage on serum vitamin D levels measured by mass spectrometry and immunoassay in reproductive age women"],"prefix":"10.1038","volume":"15","author":[{"given":"Jennifer K. Y.","family":"Ko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sammy P. L.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin K. W.","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. O.","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond H. W.","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest H. Y.","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"issue":"16\u201317","key":"2811_CR1","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1016\/j.clinbiochem.2015.08.005","volume":"48","author":"SP Wyness","year":"2015","unstructured":"Wyness, S. P. & Straseski, J. A. Performance characteristics of six automated 25-hydroxyvitamin D assays: Mind your 3s and 2s. Clin. Biochem. 48 (16\u201317), 1089\u20131096 (2015).","journal-title":"Clin. Biochem."},{"issue":"3","key":"2811_CR2","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1373\/clinchem.2011.172155","volume":"58","author":"CJ Farrell","year":"2012","unstructured":"Farrell, C. J. et al. State-of-the-art vitamin D assays: a comparison of automated immunoassays with liquid chromatography-tandem mass spectrometry methods. Clin. Chem. 58 (3), 531\u2013542 (2012).","journal-title":"Clin. Chem."},{"issue":"6","key":"2811_CR3","doi-asserted-by":"publisher","first-page":"1330","DOI":"10.1017\/S0007114507844382","volume":"99","author":"J Woo","year":"2008","unstructured":"Woo, J. et al. Very high rates of vitamin D insufficiency in women of child-bearing age living in Beijing and Hong Kong. Br. J. Nutr. 99 (6), 1330\u20131334 (2008).","journal-title":"Br. J. Nutr."},{"issue":"1","key":"2811_CR4","first-page":"231","volume":"27","author":"EW Wang","year":"2018","unstructured":"Wang, E. W. et al. Vitamin D status and cardiometabolic risk factors in young adults in Hong Kong: associations and implications. Asia Pac. J. Clin. Nutr. 27 (1), 231\u2013237 (2018).","journal-title":"Asia Pac. J. Clin. Nutr."},{"key":"2811_CR5","doi-asserted-by":"crossref","unstructured":"Ko, J. K. Y., Shi, J., Li, R. H. W., Yeung, W. S. B. & Ng, E. H. Y. 100 YEARS OF VITAMIN D: effect of serum vitamin D level before ovarian stimulation on the cumulative live birth rate of women undergoing in vitro fertilization: a retrospective analysis. Endocr. Connect. ;11(2). (2022).","DOI":"10.1530\/EC-21-0444"},{"issue":"5","key":"2811_CR6","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1530\/EJE-11-0984","volume":"166","author":"E Lerchbaum","year":"2012","unstructured":"Lerchbaum, E. & Obermayer-Pietsch, B. Vitamin D and fertility: a systematic review. Eur. J. Endocrinol. 166 (5), 765\u2013778 (2012).","journal-title":"Eur. J. Endocrinol."},{"issue":"3","key":"2811_CR7","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1097\/GCO.0000000000000065","volume":"26","author":"E Lerchbaum","year":"2014","unstructured":"Lerchbaum, E. & Rabe, T. Vitamin D and female fertility. Curr. Opin. Obst. Gynecol. 26 (3), 145\u2013150 (2014).","journal-title":"Curr. Opin. Obst. Gynecol."},{"issue":"1","key":"2811_CR8","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/s12958-018-0324-3","volume":"16","author":"J Zhao","year":"2018","unstructured":"Zhao, J. et al. Whether vitamin D was associated with clinical outcome after IVF\/ICSI: a systematic review and meta-analysis. Reprod. Biol. Endocrinol. 16 (1), 13 (2018).","journal-title":"Reprod. Biol. Endocrinol."},{"issue":"2","key":"2811_CR9","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1080\/14647273.2020.1807618","volume":"25","author":"F Iliuta","year":"2022","unstructured":"Iliuta, F., Pijoan, J. I., Lainz, L., Exposito, A. & Matorras, R. Women\u2019s vitamin D levels and IVF results: a systematic review of the literature and meta-analysis, considering three categories of vitamin status (replete, insufficient and deficient). Hum. Fertil. (Camb). 25 (2), 228\u2013246 (2022).","journal-title":"Hum. Fertil. (Camb)"},{"issue":"5","key":"2811_CR10","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.1016\/j.fertnstert.2020.05.040","volume":"114","author":"M Cozzolino","year":"2020","unstructured":"Cozzolino, M., Busnelli, A., Pellegrini, L., Riviello, E. & Vitagliano, A. How vitamin D level influences in vitro fertilization outcomes: results of a systematic review and meta-analysis. Fertil. Steril. 114 (5), 1014\u20131025 (2020).","journal-title":"Fertil. Steril."},{"issue":"1","key":"2811_CR11","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1093\/humrep\/dex326","volume":"33","author":"J Chu","year":"2018","unstructured":"Chu, J. et al. Vitamin D and assisted reproductive treatment outcome: a systematic review and meta-analysis. Hum. Reprod. 33 (1), 65\u201380 (2018).","journal-title":"Hum. Reprod."},{"issue":"2","key":"2811_CR12","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1017\/S0007114508981460","volume":"101","author":"LM Bodnar","year":"2009","unstructured":"Bodnar, L. M., Catov, J. M., Wisner, K. L. & Klebanoff, M. A. Racial and seasonal differences in 25-hydroxyvitamin D detected in maternal Sera frozen for over 40 years. Br. J. Nutr. 101 (2), 278\u2013284 (2009).","journal-title":"Br. J. Nutr."},{"issue":"1","key":"2811_CR13","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1080\/01635580903191460","volume":"62","author":"C Agborsangaya","year":"2010","unstructured":"Agborsangaya, C. et al. The effects of storage time and sampling season on the stability of serum 25-hydroxy vitamin D and Androstenedione. Nutr. Cancer. 62 (1), 51\u201357 (2010).","journal-title":"Nutr. Cancer"},{"issue":"1","key":"2811_CR14","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1373\/clinchem.2004.041954","volume":"51","author":"DM Antoniucci","year":"2005","unstructured":"Antoniucci, D. M., Black, D. M. & Sellmeyer, D. E. Serum 25-hydroxyvitamin D is unaffected by multiple freeze-thaw cycles. Clin. Chem. 51 (1), 258\u2013261 (2005).","journal-title":"Clin. Chem."},{"issue":"10","key":"2811_CR15","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1515\/cclm-2015-1034","volume":"54","author":"AA Albahrani","year":"2016","unstructured":"Albahrani, A. A., Rotarou, V., Roche, P. J. & Greaves, R. F. Analyte stability during the total testing process: studies of vitamins A, D and E by LC-MS\/MS. Clin. Chem. Lab. Med. 54 (10), 1609\u20131618 (2016).","journal-title":"Clin. Chem. Lab. Med."},{"key":"2811_CR16","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.talanta.2019.02.030","volume":"198","author":"A Mena-Bravo","year":"2019","unstructured":"Mena-Bravo, A., Calderon-Santiago, M., Luque de Castro, M. D. & Priego-Capote, F. Evaluation of short-term storage prior to analysis of vitamin D(3) and metabolites in human serum by liquid chromatography coupled to tandem mass spectrometry. Talanta 198, 344\u2013349 (2019).","journal-title":"Talanta"},{"issue":"8","key":"2811_CR17","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1016\/0895-4356(94)00232-F","volume":"48","author":"MC Ocke","year":"1995","unstructured":"Ocke, M. C. et al. Stability of blood (pro)vitamins during four years of storage at -20 degrees C: consequences for epidemiologic research. J. Clin. Epidemiol. 48 (8), 1077\u20131085 (1995).","journal-title":"J. Clin. Epidemiol."},{"issue":"10","key":"2811_CR18","doi-asserted-by":"publisher","first-page":"e389","DOI":"10.1515\/cclm-2021-0382","volume":"59","author":"E Cavalier","year":"2021","unstructured":"Cavalier, E. Long-term stability of 25-hydroxyvitamin D: importance of the analytical method and of the patient matrix. Clin. Chem. Lab. Med. 59 (10), e389\u2013e91 (2021).","journal-title":"Clin. Chem. Lab. Med."},{"issue":"Suppl","key":"2811_CR19","doi-asserted-by":"publisher","first-page":"E339","DOI":"10.1016\/j.fertnstert.2019.07.978","volume":"112","author":"EE Lara-Molina","year":"2019","unstructured":"Lara-Molina, E. E. et al. The degradation of vitamin D across time: an issue leading to unreliable results in reproductive research. Fertil. Steril. 112 (Suppl), E339\u2013E340 (2019).","journal-title":"Fertil. Steril."},{"key":"2811_CR20","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.jsbmb.2017.11.014","volume":"180","author":"O Tsuprykov","year":"2018","unstructured":"Tsuprykov, O. et al. Why should we measure free 25(OH) vitamin D? J. Steroid Biochem. Mol. Biol. 180, 87\u2013104 (2018).","journal-title":"J. Steroid Biochem. Mol. Biol."},{"issue":"4","key":"2811_CR21","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1016\/j.fertnstert.2021.02.015","volume":"116","author":"SSF Yung","year":"2021","unstructured":"Yung, S. S. F. et al. Hyaluronic acid-enriched transfer medium for frozen embryo transfer: a randomized, double-blind, controlled trial. Fertil. Steril. 116 (4), 1001\u20131009 (2021).","journal-title":"Fertil. Steril."},{"key":"2811_CR22","unstructured":"McBride, G. B. A proposal for strength-of-agreement criteria for Lin\u2019s concordance correlation. (2005). https:\/\/www.medcalc.org\/download\/pdf\/McBride2005.pdf (Accessed on May 2, 2024)."},{"issue":"1","key":"2811_CR23","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/S0378-4347(00)83564-1","volume":"381","author":"RL Norris","year":"1986","unstructured":"Norris, R. L., Thomas, M. J. & Craswell, P. W. Assessment of a two-step high-performance liquid chromatographic assay using dual-wavelength ultraviolet monitoring for 25-hydroxyergocalciferol and 25-hydroxycholecalciferol in human serum or plasma. J. Chromatogr. 381 (1), 53\u201361 (1986).","journal-title":"J. Chromatogr."},{"issue":"1","key":"2811_CR24","first-page":"100","volume":"39","author":"E Avci","year":"2020","unstructured":"Avci, E., Demir, S., Aslan, D., Nar, R. & Senol, H. Assessment of Abbott architect 25-OH vitamin D assay in different levels of vitamin D. J. Med. Biochem. 39 (1), 100\u2013107 (2020).","journal-title":"J. Med. Biochem."},{"issue":"2","key":"2811_CR25","doi-asserted-by":"publisher","first-page":"332","DOI":"10.11613\/BM.2017.036","volume":"27","author":"OC Madenci","year":"2017","unstructured":"Madenci, O. C. et al. Evaluation of new Beckman Coulter 25(OH) vitamin D assay and potential improvement of clinical interpretation. Biochem. Med. (Zagreb). 27 (2), 332\u2013341 (2017).","journal-title":"Biochem. Med. (Zagreb)"},{"issue":"4","key":"2811_CR26","doi-asserted-by":"publisher","first-page":"e22328","DOI":"10.1002\/jcla.22328","volume":"32","author":"W Annema","year":"2018","unstructured":"Annema, W., Nowak, A., von Eckardstein, A. & Saleh, L. Evaluation of the new restandardized Abbott architect 25-OH vitamin D assay in vitamin D-insufficient and vitamin D-supplemented individuals. J. Clin. Lab. Anal. 32 (4), e22328 (2018).","journal-title":"J. Clin. Lab. Anal."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-02811-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-02811-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-02811-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T16:59:35Z","timestamp":1751389175000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-02811-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["2811"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-02811-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,1]]},"assertion":[{"value":"16 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 May 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"21314"}}