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To treat these infections, the intensive use of macrocyclic lactone (ML) anthelmintics has led to the emergence of drug-resistant parasite populations worldwide. The standard method for detecting resistance, the Faecal Egg Count Reduction Test (FECRT), is susceptible to misinterpretation, leading to flawed management decisions that undermine parasite control efforts. Thus, there is a pressing need for robust resistance detection methods in field parasites. We investigated the potential of the WMicrotracker motility assay (WMA), previously unexplored in ML resistance assessment. The assay first compared ivermectin (IVM) susceptibility among wild-type Bristol N2 (N2B), IVM-selected (IVR10), and\n                    <jats:italic>nhr-8<\/jats:italic>\n                    loss-of-function (AE501;\n                    <jats:italic>nhr8(ok186)<\/jats:italic>\n                    )\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    strains. Dose-response curves indicated that IVR10 had a 2.12-fold reduction in sensitivity to IVM compared to N2B. Additionally, cross-resistance assessment showed that IVR10 exhibited decreased sensitivity to moxidectin (MOX) and eprinomectin (EPR) relative to N2B. Further investigation conducted on\n                    <jats:italic>Haemonchus contortus<\/jats:italic>\n                    revealed significant differences in drug potency between susceptible and resistant isolates, with MOX demonstrating the highest efficacy. Resistance factors (RF) highlighted the substantial resistance of the isolate collected in a farm with EPR-treatment failure. The WMA effectively discriminated susceptible from resistant isolates in both\n                    <jats:italic>C. elegans<\/jats:italic>\n                    and\n                    <jats:italic>H. contortus<\/jats:italic>\n                    . Our findings demonstrate, for the first time, the relevance of WMA as a phenotypic assay for detecting ML resistance in nematodes by measuring their motility response. This research sheds light on a novel approach for monitoring drug resistance, vital for effective parasite management strategies.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-02866-3","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T08:51:15Z","timestamp":1747990275000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of nematode susceptibility and resistance to anthelmintic drugs with a WMicrotracker motility assay"],"prefix":"10.1038","volume":"15","author":[{"given":"M\u00e9lanie","family":"Alberich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Petermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Blancfuney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Jouffroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Jacquiet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Lespine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,23]]},"reference":[{"key":"2866_CR1","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.vetpar.2013.04.005","volume":"195","author":"JL Fitzpatrick","year":"2013","unstructured":"Fitzpatrick, J. 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