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Currently, only a limited number of clinically available drugs effectively target microbial biofilms. This underscores the urgent need for the development of new sustainable therapeutic strategies to address biofilm-associated infections. Developing a sustainable and biodegradable preparation for eradicating microbial biofilms is critically important. In this study, chitosan was extracted from shrimp shell waste and utilized to prepare salicylic acid-loaded chitosan nanoparticles (NPs) using various synthesis methods. The particle size of the prepared nanoparticles ranged from 287.4 to 226.3\u00a0nm, with zeta potential values between +\u200936.6 and +\u200941.3 mV, indicating good stability. The nanoparticles demonstrated safety, with half maximal inhibitory concentration (IC\n                    <jats:sub>50<\/jats:sub>\n                    ) values ranging from 1009 to 1346\u00a0\u00b5g\/mL. The combination of chitosan and salicylic acid exhibited significant antibiofilm activity against\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , with particularly high efficacy against\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , achieving up to 85% biofilm inhibition. While the particle size and antibiofilm activity of the nanoforms showed minimal differences, formulation M4, using sodium alginate, stands out as the most eco-friendly option. This study highlights the potential of bio-sustainable chitosan-based formulations for combating biofilm formation and addressing antimicrobial resistance.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-03355-3","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T04:30:12Z","timestamp":1748838612000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Isolation and characterization of Chitosan from shrimp shell waste and the sustainable preparation of salicylic acid-loaded Chitosan nanoparticles for antibiofilm applications"],"prefix":"10.1038","volume":"15","author":[{"given":"Habiba A.","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9501-8059","authenticated-orcid":false,"given":"Yousra A.","family":"El-Maradny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal A.","family":"Shalaby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany","family":"El-Menshawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeer E.","family":"Abd EL-Wahab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"3355_CR1","unstructured":"O\u2019Neill, J. 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