{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T21:08:15Z","timestamp":1781730495550,"version":"3.54.5"},"reference-count":55,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Hebei Natural Science Foundation Youth Fund","award":["H2023206329"],"award-info":[{"award-number":["H2023206329"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-04388-4","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T15:45:09Z","timestamp":1748879109000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The dual effects of iron deficiency on intervertebral disc development and on acute injury-induced disc degeneration"],"prefix":"10.1038","volume":"15","author":[{"given":"Hengrui","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaomeng","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenchen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongran","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanzhong","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"4388_CR1","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/s0140-6736(20)32594-0","volume":"397","author":"SR Pasricha","year":"2021","unstructured":"Pasricha, S. R., Tye-Din, J., Muckenthaler, M. U. & Swinkels, D. W. Iron deficiency. Lancet (Lond., Engl.) 397, 233\u2013248. https:\/\/doi.org\/10.1016\/s0140-6736(20)32594-0 (2021).","journal-title":"Lancet (Lond., Engl.)"},{"key":"4388_CR2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3534","author":"S Ropele","year":"2017","unstructured":"Ropele, S. & Langkammer, C. Iron quantification with susceptibility. NMR Biomed. https:\/\/doi.org\/10.1002\/nbm.3534 (2017).","journal-title":"NMR Biomed."},{"key":"4388_CR3","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1056\/NEJMra1004967","volume":"366","author":"RE Fleming","year":"2012","unstructured":"Fleming, R. E. & Ponka, P. Iron overload in human disease. N. Engl. J. Med. 366, 348\u2013359. https:\/\/doi.org\/10.1056\/NEJMra1004967 (2012).","journal-title":"N. Engl. J. Med."},{"key":"4388_CR4","doi-asserted-by":"publisher","first-page":"1986","DOI":"10.1056\/nejm199912233412607","volume":"341","author":"NC Andrews","year":"1999","unstructured":"Andrews, N. C. Disorders of iron metabolism. N. Engl. J. Med. 341, 1986\u20131995. https:\/\/doi.org\/10.1056\/nejm199912233412607 (1999).","journal-title":"N. Engl. J. Med."},{"key":"4388_CR5","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1182\/blood-2013-06-508325","volume":"123","author":"NJ Kassebaum","year":"2014","unstructured":"Kassebaum, N. J. et al. A systematic analysis of global anemia burden from 1990 to 2010. Blood 123, 615\u2013624. https:\/\/doi.org\/10.1182\/blood-2013-06-508325 (2014).","journal-title":"Blood"},{"key":"4388_CR6","doi-asserted-by":"publisher","first-page":"359s","DOI":"10.3945\/ajcn.116.141762","volume":"106","author":"SM Namaste","year":"2017","unstructured":"Namaste, S. M. et al. Adjusting ferritin concentrations for inflammation: Biomarkers reflecting inflammation and nutritional determinants of anemia (BRINDA) project. Am. J. Clin. Nutr. 106, 359s\u2013371s. https:\/\/doi.org\/10.3945\/ajcn.116.141762 (2017).","journal-title":"Am. J. Clin. Nutr."},{"key":"4388_CR7","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.3945\/ajcn.110.007195","volume":"93","author":"Z Mei","year":"2011","unstructured":"Mei, Z. et al. Assessment of iron status in US pregnant women from the national health and nutrition examination survey (NHANES), 1999\u20132006. Am. J. Clin. Nutr. 93, 1312\u20131320. https:\/\/doi.org\/10.3945\/ajcn.110.007195 (2011).","journal-title":"Am. J. Clin. Nutr."},{"key":"4388_CR8","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1016\/s0140-6736(17)32154-2","volume":"390","author":"T Vos","year":"2017","unstructured":"Vos, T. et al. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990\u20132016: a systematic analysis for the global burden of disease study 2016. Lancet (Lond., Engl.) 390, 1211\u20131259. https:\/\/doi.org\/10.1016\/s0140-6736(17)32154-2 (2017).","journal-title":"Lancet (Lond., Engl.)"},{"key":"4388_CR9","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1001\/jamacardio.2019.1739","volume":"4","author":"N Grote Beverborg","year":"2019","unstructured":"Grote Beverborg, N. et al. Differences in clinical profile and outcomes of low iron storage vs defective iron utilization in patients with heart failure: Results From the DEFINE-HF and BIOSTAT-CHF studies. JAMA Cardiol. 4, 696\u2013701. https:\/\/doi.org\/10.1001\/jamacardio.2019.1739 (2019).","journal-title":"JAMA Cardiol."},{"key":"4388_CR10","doi-asserted-by":"publisher","DOI":"10.1136\/bcr-2019-229942","author":"CA Perera","year":"2019","unstructured":"Perera, C. A., Biggers, R. P. & Robertson, A. Deceitful red-flag: angina secondary to iron deficiency anaemia as a presenting complaint for underlying malignancy. BMJ Case Rep. https:\/\/doi.org\/10.1136\/bcr-2019-229942 (2019).","journal-title":"BMJ Case Rep."},{"key":"4388_CR11","doi-asserted-by":"publisher","first-page":"1396","DOI":"10.1016\/s0140-6736(11)61381-0","volume":"378","author":"KM Musallam","year":"2011","unstructured":"Musallam, K. M. et al. Preoperative anaemia and postoperative outcomes in non-cardiac surgery: A retrospective cohort study. Lancet (Lond., Engl.) 378, 1396\u20131407. https:\/\/doi.org\/10.1016\/s0140-6736(11)61381-0 (2011).","journal-title":"Lancet (Lond., Engl.)"},{"key":"4388_CR12","doi-asserted-by":"publisher","first-page":"3135","DOI":"10.1093\/jn\/131.10.3135","volume":"132","author":"DM Medeiros","year":"2002","unstructured":"Medeiros, D. M., Plattner, A., Jennings, D. & Stoecker, B. Bone morphology, strength and density are compromised in iron-deficient rats and exacerbated by calcium restriction. J. Nutr. 132, 3135\u20133141. https:\/\/doi.org\/10.1093\/jn\/131.10.3135 (2002).","journal-title":"J. Nutr."},{"key":"4388_CR13","doi-asserted-by":"publisher","first-page":"3061","DOI":"10.1093\/jn\/134.11.3061","volume":"134","author":"DM Medeiros","year":"2004","unstructured":"Medeiros, D. M., Stoecker, B., Plattner, A., Jennings, D. & Haub, M. Iron deficiency negatively affects vertebrae and femurs of rats independently of energy intake and body weight. J. Nutr. 134, 3061\u20133067. https:\/\/doi.org\/10.1093\/jn\/134.11.3061 (2004).","journal-title":"J. Nutr."},{"key":"4388_CR14","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1177\/153537020623100403","volume":"231","author":"M Parelman","year":"2006","unstructured":"Parelman, M., Stoecker, B., Baker, A. & Medeiros, D. Iron restriction negatively affects bone in female rats and mineralization of hFOB osteoblast cells. Exp. Biol. Med. (Maywood, N.J.) 231, 378\u2013386. https:\/\/doi.org\/10.1177\/153537020623100403 (2006).","journal-title":"Exp. Biol. Med. (Maywood, N.J.)"},{"key":"4388_CR15","doi-asserted-by":"publisher","DOI":"10.1002\/jbm4.10529","volume":"5","author":"DF Edwards 3rd","year":"2021","unstructured":"Edwards, D. F. 3rd. et al. Differential iron requirements for osteoblast and adipocyte differentiation. JBMR Plus 5, e10529. https:\/\/doi.org\/10.1002\/jbm4.10529 (2021).","journal-title":"JBMR Plus"},{"key":"4388_CR16","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1007\/s12011-012-9511-9","volume":"150","author":"GY Zhao","year":"2012","unstructured":"Zhao, G. Y. et al. A comparison of the biological activities of human osteoblast hFOB1.19 between iron excess and iron deficiency. Biol. Trace Elem. Res. 150, 487\u2013495. https:\/\/doi.org\/10.1007\/s12011-012-9511-9 (2012).","journal-title":"Biol. Trace Elem. Res."},{"key":"4388_CR17","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1007\/s00198-005-0033-6","volume":"17","author":"R Abraham","year":"2006","unstructured":"Abraham, R. et al. Dietary determinants of post-menopausal bone loss at the lumbar spine: A possible beneficial effect of iron. Osteoporos. Int. 17, 1165\u20131173. https:\/\/doi.org\/10.1007\/s00198-005-0033-6 (2006).","journal-title":"Osteoporos. Int."},{"key":"4388_CR18","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1007\/s00198-004-1739-6","volume":"16","author":"M Cesari","year":"2005","unstructured":"Cesari, M. et al. Bone density and hemoglobin levels in older persons: results from the InCHIANTI study. Osteoporos. Int. 16, 691\u2013699. https:\/\/doi.org\/10.1007\/s00198-004-1739-6 (2005).","journal-title":"Osteoporos. Int."},{"key":"4388_CR19","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/s12011-014-0031-7","volume":"160","author":"J Zheng","year":"2014","unstructured":"Zheng, J., Mao, X., Ling, J., He, Q. & Quan, J. Low serum levels of zinc, copper, and iron as risk factors for osteoporosis: A meta-analysis. Biol. Trace Elem. Res. 160, 15\u201323. https:\/\/doi.org\/10.1007\/s12011-014-0031-7 (2014).","journal-title":"Biol. Trace Elem. Res."},{"key":"4388_CR20","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1002\/hep.1840150218","volume":"15","author":"H Ikeda","year":"1992","unstructured":"Ikeda, H., Wu, G. Y. & Wu, C. H. Evidence that an iron chelator regulates collagen synthesis by decreasing the stability of procollagen mRNA. Hepatology (Baltimore, MD) 15, 282\u2013287. https:\/\/doi.org\/10.1002\/hep.1840150218 (1992).","journal-title":"Hepatology (Baltimore, MD)"},{"key":"4388_CR21","doi-asserted-by":"publisher","first-page":"1895","DOI":"10.1093\/jn\/137.8.1895","volume":"137","author":"CH Hill","year":"2007","unstructured":"Hill, C. H. et al. Dietary iron deficiency compromises normal development of elastic fibers in the aorta and lungs of chicks. J. Nutr. 137, 1895\u20131900. https:\/\/doi.org\/10.1093\/jn\/137.8.1895 (2007).","journal-title":"J. Nutr."},{"key":"4388_CR22","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1128\/mcb.3.2.241-249.1983","volume":"3","author":"LB Rowe","year":"1983","unstructured":"Rowe, L. B. & Schwarz, R. I. Role of procollagen mRNA levels in controlling the rate of procollagen synthesis. Mol. Cell. Biol. 3, 241\u2013249. https:\/\/doi.org\/10.1128\/mcb.3.2.241-249.1983 (1983).","journal-title":"Mol. Cell. Biol."},{"key":"4388_CR23","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1111\/j.1365-2141.1979.tb03682.x","volume":"41","author":"J Hunt","year":"1979","unstructured":"Hunt, J., Richards, R. J., Harwood, R. & Jacobs, A. The effect of desferrioxamine on fibroblasts and collagen formation in cell cultures. Br. J. Haematol. 41, 69\u201376. https:\/\/doi.org\/10.1111\/j.1365-2141.1979.tb03682.x (1979).","journal-title":"Br. J. Haematol."},{"key":"4388_CR24","doi-asserted-by":"publisher","first-page":"1861","DOI":"10.1177\/03635465211005754","volume":"49","author":"AJ Theruvath","year":"2021","unstructured":"Theruvath, A. J. et al. Ascorbic acid and iron supplement treatment improves stem cell-mediated cartilage regeneration in a Minipig model. Am. J. Sports Med. 49, 1861\u20131870. https:\/\/doi.org\/10.1177\/03635465211005754 (2021).","journal-title":"Am. J. Sports Med."},{"key":"4388_CR25","first-page":"3430","volume":"10","author":"C Zhang","year":"2018","unstructured":"Zhang, C. et al. Iron deficiency accelerates intervertebral disc degeneration through affecting the stability of DNA polymerase epsilon complex. Am. J. Transl. Res. 10, 3430\u20133442 (2018).","journal-title":"Am. J. Transl. Res."},{"key":"4388_CR26","doi-asserted-by":"publisher","first-page":"2059","DOI":"10.1007\/s00586-023-07652-1","volume":"32","author":"H Chang","year":"2023","unstructured":"Chang, H. et al. Association between anemia and lumbar disc degeneration in patients with low back pain: An observational retrospective study. Eur. Spine J.: Off. Publ. Eur. Spine Soc., Eur. Spinal Deform. Soc. Eur. Sec. Cerv. Spine Res. Soc. 32, 2059\u20132068. https:\/\/doi.org\/10.1007\/s00586-023-07652-1 (2023).","journal-title":"Eur. Spine J.: Off. Publ. Eur. Spine Soc., Eur. Spinal Deform. Soc. Eur. Sec. Cerv. Spine Res. Soc."},{"key":"4388_CR27","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1007\/s00586-022-07259-y","volume":"31","author":"H Chang","year":"2022","unstructured":"Chang, H., Gao, X., Li, X., Zhao, R. & Ding, W. Anemia was associated with multilevel lumbar disc degeneration in patients with low back pain: a single-center retrospective study. Eur. Spine J.: Off. Publ. Eur. Spine Soc., Eur. Spinal Deform. Soc. Eur. Sec. Cerv. Spine Res. Soc. 31, 1897\u20131905. https:\/\/doi.org\/10.1007\/s00586-022-07259-y (2022).","journal-title":"Eur. Spine J.: Off. Publ. Eur. Spine Soc., Eur. Spinal Deform. Soc. Eur. Sec. Cerv. Spine Res. Soc."},{"key":"4388_CR28","doi-asserted-by":"publisher","first-page":"1340","DOI":"10.1093\/jn\/107.7.1340","volume":"107","author":"CB Diet","year":"1977","unstructured":"Diet, C. B., Diet, U. & Diet, N. P. Report of the American Institute of Nurtition ad hoc Committee on Standards for Nutritional Studies. J. Nutr. 107, 1340\u20131348. https:\/\/doi.org\/10.1093\/jn\/107.7.1340 (1977).","journal-title":"J. Nutr."},{"key":"4388_CR29","doi-asserted-by":"publisher","first-page":"1873","DOI":"10.1097\/00007632-200109010-00011","volume":"26","author":"CW Pfirrmann","year":"2001","unstructured":"Pfirrmann, C. W., Metzdorf, A., Zanetti, M., Hodler, J. & Boos, N. Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine 26, 1873\u20131878. https:\/\/doi.org\/10.1097\/00007632-200109010-00011 (2001).","journal-title":"Spine"},{"key":"4388_CR30","doi-asserted-by":"publisher","first-page":"5051","DOI":"10.1038\/s41467-018-07360-1","volume":"9","author":"ML Ji","year":"2018","unstructured":"Ji, M. L. et al. Preclinical development of a microRNA-based therapy for intervertebral disc degeneration. Nat. Commun. 9, 5051. https:\/\/doi.org\/10.1038\/s41467-018-07360-1 (2018).","journal-title":"Nat. Commun."},{"key":"4388_CR31","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1002\/ar.24519","volume":"304","author":"IO Abdel Fattah","year":"2021","unstructured":"Abdel Fattah, I. O. & Nasr El-Din, W. A. Granulocyte-colony stimulating factor improves intervertebral disc degeneration in experimental adult male rats: A microscopic and radiological study. Anat. Rec. (Hoboken, N.J.:2007) 304, 787\u2013802. https:\/\/doi.org\/10.1002\/ar.24519 (2021).","journal-title":"Anat. Rec. (Hoboken, N.J.:2007)"},{"key":"4388_CR32","doi-asserted-by":"publisher","first-page":"1316","DOI":"10.1177\/1535370216648805","volume":"241","author":"DM Medeiros","year":"2016","unstructured":"Medeiros, D. M. Copper, iron, and selenium dietary deficiencies negatively impact skeletal integrity: A review. Exp. Biol. Med. (Maywood, N.J.) 241, 1316\u20131322. https:\/\/doi.org\/10.1177\/1535370216648805 (2016).","journal-title":"Exp. Biol. Med. (Maywood, N.J.)"},{"key":"4388_CR33","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.ab.2008.11.046","volume":"386","author":"KL Gorres","year":"2009","unstructured":"Gorres, K. L. & Raines, R. T. Direct and continuous assay for prolyl 4-hydroxylase. Anal. Biochem. 386, 181\u2013185. https:\/\/doi.org\/10.1016\/j.ab.2008.11.046 (2009).","journal-title":"Anal. Biochem."},{"key":"4388_CR34","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1007\/s00394-011-0212-5","volume":"51","author":"J D\u00edaz-Castro","year":"2012","unstructured":"D\u00edaz-Castro, J. et al. Severe nutritional iron-deficiency anaemia has a negative effect on some bone turnover biomarkers in rats. Eur. J. Nutr. 51, 241\u2013247. https:\/\/doi.org\/10.1007\/s00394-011-0212-5 (2012).","journal-title":"Eur. J. Nutr."},{"key":"4388_CR35","doi-asserted-by":"publisher","first-page":"203","DOI":"10.3233\/bir-1978-153-409","volume":"15","author":"JP Urban","year":"1978","unstructured":"Urban, J. P., Holm, S. & Maroudas, A. Diffusion of small solutes into the intervertebral disc: as in vivo study. Biorheology 15, 203\u2013221. https:\/\/doi.org\/10.3233\/bir-1978-153-409 (1978).","journal-title":"Biorheology"},{"key":"4388_CR36","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1111\/j.1469-7580.2007.00816.x","volume":"211","author":"M van der Werf","year":"2007","unstructured":"van der Werf, M., Lezuo, P., Maissen, O., van Donkelaar, C. C. & Ito, K. Inhibition of vertebral endplate perfusion results in decreased intervertebral disc intranuclear diffusive transport. J. Anat. 211, 769\u2013774. https:\/\/doi.org\/10.1111\/j.1469-7580.2007.00816.x (2007).","journal-title":"J. Anat."},{"key":"4388_CR37","doi-asserted-by":"publisher","first-page":"2654","DOI":"10.1097\/01.brs.0000148014.15210.64","volume":"29","author":"S Rajasekaran","year":"2004","unstructured":"Rajasekaran, S. et al. ISSLS prize winner: A study of diffusion in human lumbar discs: A serial magnetic resonance imaging study documenting the influence of the endplate on diffusion in normal and degenerate discs. Spine 29, 2654\u20132667. https:\/\/doi.org\/10.1097\/01.brs.0000148014.15210.64 (2004).","journal-title":"Spine"},{"key":"4388_CR38","doi-asserted-by":"publisher","first-page":"2769","DOI":"10.1016\/j.jbiomech.2012.08.044","volume":"45","author":"Q Zhu","year":"2012","unstructured":"Zhu, Q., Jackson, A. R. & Gu, W. Y. Cell viability in intervertebral disc under various nutritional and dynamic loading conditions: 3d finite element analysis. J. Biomech. 45, 2769\u20132777. https:\/\/doi.org\/10.1016\/j.jbiomech.2012.08.044 (2012).","journal-title":"J. Biomech."},{"key":"4388_CR39","doi-asserted-by":"publisher","first-page":"1330","DOI":"10.1016\/j.jbiomech.2010.01.023","volume":"43","author":"A Shirazi-Adl","year":"2010","unstructured":"Shirazi-Adl, A., Taheri, M. & Urban, J. P. Analysis of cell viability in intervertebral disc: Effect of endplate permeability on cell population. J. Biomech. 43, 1330\u20131336. https:\/\/doi.org\/10.1016\/j.jbiomech.2010.01.023 (2010).","journal-title":"J. Biomech."},{"key":"4388_CR40","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1186\/s10020-021-00368-2","volume":"27","author":"L Shan","year":"2021","unstructured":"Shan, L. et al. Increased hemoglobin and heme in MALDI-TOF MS analysis induce ferroptosis and promote degeneration of herniated human nucleus pulposus. Mol. Med. (Cambridge, Mass) 27, 103. https:\/\/doi.org\/10.1186\/s10020-021-00368-2 (2021).","journal-title":"Mol. Med. (Cambridge, Mass)"},{"key":"4388_CR41","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.freeradbiomed.2022.08.018","volume":"190","author":"W Wang","year":"2022","unstructured":"Wang, W. et al. Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes. Free Radical Biol. Med. 190, 234\u2013246. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2022.08.018 (2022).","journal-title":"Free Radical Biol. Med."},{"key":"4388_CR42","doi-asserted-by":"publisher","first-page":"6670497","DOI":"10.1155\/2021\/6670497","volume":"2021","author":"S Lu","year":"2021","unstructured":"Lu, S. et al. Ferroportin-dependent iron homeostasis protects against oxidative stress-induced nucleus pulposus cell ferroptosis and ameliorates intervertebral disc degeneration in vivo. Oxid. Med. Cell. Longev. 2021, 6670497. https:\/\/doi.org\/10.1155\/2021\/6670497 (2021).","journal-title":"Oxid. Med. Cell. Longev."},{"key":"4388_CR43","doi-asserted-by":"publisher","first-page":"4235126","DOI":"10.1155\/2022\/4235126","volume":"2022","author":"Y Li","year":"2022","unstructured":"Li, Y. et al. Silencing ATF3 might delay TBHP-induced intervertebral disc degeneration by repressing NPC ferroptosis, apoptosis, and ECM degradation. Oxid. Med. Cell. Longev. 2022, 4235126. https:\/\/doi.org\/10.1155\/2022\/4235126 (2022).","journal-title":"Oxid. Med. Cell. Longev."},{"key":"4388_CR44","doi-asserted-by":"publisher","first-page":"2486","DOI":"10.1097\/BRS.0b013e3181b26ed1","volume":"34","author":"JH Kim","year":"2009","unstructured":"Kim, J. H. et al. Differentiation of intervertebral notochordal cells through live automated cell imaging system in vitro. Spine 34, 2486\u20132493. https:\/\/doi.org\/10.1097\/BRS.0b013e3181b26ed1 (2009).","journal-title":"Spine"},{"key":"4388_CR45","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.spinee.2008.05.003","volume":"9","author":"KW Kim","year":"2009","unstructured":"Kim, K. W. et al. Notochordal cells stimulate migration of cartilage end plate chondrocytes of the intervertebral disc in in vitro cell migration assays. Spine J.: Off. J. N. Am. Spine Soc. 9, 323\u2013329. https:\/\/doi.org\/10.1016\/j.spinee.2008.05.003 (2009).","journal-title":"Spine J.: Off. J. N. Am. Spine Soc."},{"key":"4388_CR46","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/scrt18","volume":"1","author":"CL Korecki","year":"2010","unstructured":"Korecki, C. L., Taboas, J. M., Tuan, R. S. & Iatridis, J. C. Notochordal cell conditioned medium stimulates mesenchymal stem cell differentiation toward a young nucleus pulposus phenotype. Stem Cell Res. Ther. 1, 18. https:\/\/doi.org\/10.1186\/scrt18 (2010).","journal-title":"Stem Cell Res. Ther."},{"key":"4388_CR47","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.1016\/j.spinee.2015.02.003","volume":"15","author":"D Purmessur","year":"2015","unstructured":"Purmessur, D. et al. Intact glycosaminoglycans from intervertebral disc-derived notochordal cell-conditioned media inhibit neurite growth while maintaining neuronal cell viability. Spine J.: Off. J. N. Am. Spine Soc. 15, 1060\u20131069. https:\/\/doi.org\/10.1016\/j.spinee.2015.02.003 (2015).","journal-title":"Spine J.: Off. J. N. Am. Spine Soc."},{"key":"4388_CR48","doi-asserted-by":"publisher","first-page":"57800","DOI":"10.18632\/oncotarget.18101","volume":"8","author":"F Wang","year":"2017","unstructured":"Wang, F. et al. Formation, function, and exhaustion of notochordal cytoplasmic vacuoles within intervertebral disc: current understanding and speculation. Oncotarget 8, 57800\u201357812. https:\/\/doi.org\/10.18632\/oncotarget.18101 (2017).","journal-title":"Oncotarget"},{"key":"4388_CR49","doi-asserted-by":"publisher","first-page":"1026","DOI":"10.1002\/art.24407","volume":"60","author":"T Guehring","year":"2009","unstructured":"Guehring, T. et al. Notochordal intervertebral disc cells: sensitivity to nutrient deprivation. Arthritis Rheum. 60, 1026\u20131034. https:\/\/doi.org\/10.1002\/art.24407 (2009).","journal-title":"Arthritis Rheum."},{"key":"4388_CR50","doi-asserted-by":"publisher","first-page":"2014","DOI":"10.1016\/j.bbagen.2014.07.013","volume":"3181\u20133189","author":"SS Sivan","year":"1840","unstructured":"Sivan, S. S., Wachtel, E. & Roughley, P. Structure, function, aging and turnover of aggrecan in the intervertebral disc. Biochem. Biophys. Acta. 3181\u20133189, 2014. https:\/\/doi.org\/10.1016\/j.bbagen.2014.07.013 (1840).","journal-title":"Biochem. Biophys. Acta."},{"key":"4388_CR51","doi-asserted-by":"publisher","first-page":"58","DOI":"10.22203\/ecm.v008a06","volume":"8","author":"F Mwale","year":"2004","unstructured":"Mwale, F., Roughley, P. & Antoniou, J. Distinction between the extracellular matrix of the nucleus pulposus and hyaline cartilage: A requisite for tissue engineering of intervertebral disc. Eur. Cells Mater. 8, 58\u201363. https:\/\/doi.org\/10.22203\/ecm.v008a06 (2004).","journal-title":"Eur. Cells Mater."},{"key":"4388_CR52","doi-asserted-by":"publisher","first-page":"C751","DOI":"10.1152\/ajpcell.00271.2019","volume":"318","author":"M He","year":"2020","unstructured":"He, M. et al. P14ARF inhibits regional inflammation and vascularization in intervertebral disc degeneration by upregulating TIMP3. Am. J. Physiol. Cell Physiol. 318, C751-c761. https:\/\/doi.org\/10.1152\/ajpcell.00271.2019 (2020).","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"4388_CR53","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.jos.2018.08.006","volume":"24","author":"Y Zhang","year":"2019","unstructured":"Zhang, Y. et al. Effect of hyperlipidaemia to accelerate intervertebral disc degeneration in the injured rat caudal disc model. J. Orthop. Sci.: Off. J. Jpn. Orthop. Assoc. 24, 42\u201349. https:\/\/doi.org\/10.1016\/j.jos.2018.08.006 (2019).","journal-title":"J. Orthop. Sci.: Off. J. Jpn. Orthop. Assoc."},{"key":"4388_CR54","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1016\/j.neuint.2012.07.024","volume":"61","author":"CO Arent","year":"2012","unstructured":"Arent, C. O. et al. Synergist effects of n-acetylcysteine and deferoxamine treatment on behavioral and oxidative parameters induced by chronic mild stress in rats. Neurochem. Int. 61, 1072\u20131080. https:\/\/doi.org\/10.1016\/j.neuint.2012.07.024 (2012).","journal-title":"Neurochem. Int."},{"key":"4388_CR55","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1007\/s12192-020-01142-9","volume":"25","author":"N Khoshlahni","year":"2020","unstructured":"Khoshlahni, N., Sagha, M., Mirzapour, T., Zarif, M. N. & Mohammadzadeh-Vardin, M. Iron depletion with deferoxamine protects bone marrow-derived mesenchymal stem cells against oxidative stress-induced apoptosis. Cell Stress Chaperones 25, 1059\u20131069. https:\/\/doi.org\/10.1007\/s12192-020-01142-9 (2020).","journal-title":"Cell Stress Chaperones"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-04388-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-04388-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-04388-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T15:45:09Z","timestamp":1748879109000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-04388-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,2]]},"references-count":55,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["4388"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-04388-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,2]]},"assertion":[{"value":"20 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 May 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 June 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"19323"}}