{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T11:25:23Z","timestamp":1764588323155,"version":"3.46.0"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-06210-7","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T04:15:30Z","timestamp":1764562530000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A pilot study on the prevalence and patterns of haemoglobinopathies in Datia District, Madhya Pradesh, India"],"prefix":"10.1038","volume":"15","author":[{"given":"S.","family":"Rajasubramaniam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nalini","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Lokeswara","family":"Balakrishna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Archana","family":"Kumawat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajesh","family":"Gaur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amita","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aparup","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"key":"6210_CR1","first-page":"498","volume":"134","author":"S Fucharoen","year":"2011","unstructured":"Fucharoen, S. & Winichagoon, P. Haemoglobinopathies in Southeast Asia. Indian J. Med. Res. 134, 498\u2013506 (2011).","journal-title":"Indian J. Med. Res."},{"key":"6210_CR2","unstructured":"Bhatia, H. M. & Rao, V. R., editors. Mumbai: Institute of Immunohaematology, Indian Council of Medical Research. Genetic atlas of the Indian tribes (1986)."},{"key":"6210_CR3","first-page":"493","volume":"134","author":"D Weatherall","year":"2011","unstructured":"Weatherall, D. The inherited disorders of haemoglobin: An increasingly neglected global health burden. Indian J. Med. Res. 134, 493\u2013497 (2011).","journal-title":"Indian J. Med. Res."},{"key":"6210_CR4","doi-asserted-by":"publisher","first-page":"926000","DOI":"10.3389\/fpubh.2022.926000","volume":"10","author":"N Mishra","year":"2022","unstructured":"Mishra, N. et al. Profile of diseases prevalent in Datia District of Madhya Pradesh, India. Front. Public Health 10, 926000. https:\/\/doi.org\/10.3389\/fpubh.2022.926000 (2022).","journal-title":"Front. Public Health"},{"issue":"1","key":"6210_CR5","first-page":"27","volume":"27","author":"RJ Trent","year":"2006","unstructured":"Trent, R. J. Diagnosis of the haemoglobinopathies. Clin. Biochem. Rev. 27(1), 27 (2006).","journal-title":"Clin. Biochem. Rev."},{"issue":"9","key":"6210_CR6","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1136\/jcp.23.9.781","volume":"23","author":"RG Huntsman","year":"1970","unstructured":"Huntsman, R. G., Barclay, G. P., Canning, D. M. & Yawson, G. I. A rapid whole blood solubility test to differentiate the sickle cell trait from sickle cell anaemia. J. Clin. Pathol. 23(9), 781\u2013783. https:\/\/doi.org\/10.1136\/jcp.23.9.781 (1970).","journal-title":"J. Clin. Pathol."},{"issue":"3","key":"6210_CR7","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/0009-8981(68)90302-1","volume":"20","author":"A Pabis","year":"1968","unstructured":"Pabis, A., Sulis, E., Alessio, L. & Mannucci, P. M. Cellogel electrophoresis of Haemoglobins. Clin. Chim. Acta 20(3), 449\u2013453. https:\/\/doi.org\/10.1016\/0009-8981(68)90302-1 (1968).","journal-title":"Clin. Chim. Acta"},{"key":"6210_CR8","volume-title":"Practical Haematology","year":"1991","unstructured":"Dacie, J. V. & Lewis, S. M. (eds) Practical Haematology 7th edn. (Churchill Livingstone, 1991).","edition":"7"},{"issue":"8","key":"6210_CR9","doi-asserted-by":"publisher","first-page":"1284","DOI":"10.1093\/clinchem\/46.8.1284","volume":"46","author":"GM Clarke","year":"2000","unstructured":"Clarke, G. M. & Higgins, T. N. Laboratory Investigation of Hemoglobinopathies and Thalassemias: Review and Update. Clin. Chem. 46(8), 1284\u20131290 (2000).","journal-title":"Clin. Chem."},{"key":"6210_CR10","doi-asserted-by":"publisher","DOI":"10.1038\/194192a0","author":"RE Benstein","year":"1962","unstructured":"Benstein, R. E. A rapid screening dye test for the detection of G6PD deficiency in red cells. Nature https:\/\/doi.org\/10.1038\/194192a0 (1962).","journal-title":"Nature"},{"key":"6210_CR11","first-page":"39","volume":"3","author":"RS Pavri","year":"1979","unstructured":"Pavri, R. S., Vasantha, K. & Baxi, A. J. Usefulness of dicholoro-indophenol and fluorescent spot screening for G6PD deficiency. Acta Anthropogenet. 3, 39 (1979).","journal-title":"Acta Anthropogenet."},{"issue":"9","key":"6210_CR12","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1080\/10245332.2016.1148893","volume":"21","author":"R Yadav","year":"2016","unstructured":"Yadav, R., Lazarus, M. & Ghanghoria, P. Sickle cell disease in Madhya Pradesh, Central India: A comparison of clinical profile of sickle cell homozygote versus sickle-beta thalassaemia individuals. Hematology 21(9), 558\u2013563. https:\/\/doi.org\/10.1080\/10245332.2016.1148893 (2016).","journal-title":"Hematology"},{"key":"6210_CR13","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/s11568-010-9132-3","volume":"3","author":"S Sinha","year":"2009","unstructured":"Sinha, S. et al. Profiling \u03b2-thalassaemia mutations in India at state and regional levels: Implications for genetic education, screening and counselling programmes. Hugo J. 3, 51\u201362. https:\/\/doi.org\/10.1007\/s11568-010-9132-3 (2009).","journal-title":"Hugo J."},{"key":"6210_CR14","doi-asserted-by":"publisher","first-page":"100916","DOI":"10.1016\/j.cegh.2021.100916","volume":"13","author":"SS Mohanty","year":"2022","unstructured":"Mohanty, S. S. et al. Prevalence of sickle cell anemia, \u03b2-thalassemia and glucose-6-phosphate dehydrogenase deficiency among the tribal population residing in the Aravali hills of Sirohi region of Rajasthan state. Clin. Epidemiol. Glob. Health 13, 100916. https:\/\/doi.org\/10.1016\/j.cegh.2021.100916 (2022).","journal-title":"Clin. Epidemiol. Glob. Health"},{"key":"6210_CR15","doi-asserted-by":"publisher","first-page":"1198","DOI":"10.1016\/s0140-6736(86)92205-1","volume":"328","author":"BC Kar","year":"1986","unstructured":"Kar, B. C. et al. Sickle cell disease in Orissa State, India. The Lancet 328, 1198\u20131201. https:\/\/doi.org\/10.1016\/s0140-6736(86)92205-1 (1986).","journal-title":"The Lancet"},{"issue":"12","key":"6210_CR16","doi-asserted-by":"publisher","first-page":"LC9","DOI":"10.7860\/JCDR\/2016\/22010.9002","volume":"10","author":"AB Teli","year":"2016","unstructured":"Teli, A. B., Deori, R. & Saikia, S. P. Haemoglobinopathies and \u03b2-thalassaemia among the tribals working in the tea Gardens of Assam, India. J. Clin. Diag. Res. 10(12), LC9\u2013LC22. https:\/\/doi.org\/10.7860\/JCDR\/2016\/22010.9002 (2016).","journal-title":"J. Clin. Diag. Res."},{"issue":"7","key":"6210_CR17","doi-asserted-by":"publisher","first-page":"2767","DOI":"10.4103\/jfmpc.jfmpc_157_24","volume":"13","author":"BG Pichamuthu","year":"2024","unstructured":"Pichamuthu, B. G., Arunachalam, K. D. & Kosalram, K. Assessing NESTROFT as a preliminary screening tool for thalassemia in the Malayali tribes of Dharmapuri district, Tamil Nadu, India. J. Fam. Med. Prim. Care 13(7), 2767\u20132771 (2024).","journal-title":"J. Fam. Med. Prim. Care"},{"issue":"4","key":"6210_CR18","doi-asserted-by":"publisher","first-page":"854","DOI":"10.4269\/ajtmh.14-0194","volume":"91","author":"N LaRue","year":"2014","unstructured":"LaRue, N. et al. Comparison of quantitative and qualitative tests for glucose-6-phosphate dehydrogenase deficiency. Am. J. Trop. Med. Hyg. 91(4), 854\u2013861. https:\/\/doi.org\/10.4269\/ajtmh.14-0194 (2014).","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"12","key":"6210_CR19","first-page":"954","volume":"39","author":"BC Kar","year":"1991","unstructured":"Kar, B. C. Sickle cell disease in India. JAPI 39(12), 954\u2013960 (1991).","journal-title":"JAPI"},{"key":"6210_CR20","doi-asserted-by":"publisher","unstructured":"Mishra, N., Rajasubramaniam, S., Balakrishna, S.L., Kumawat, A., Gaur, R., Sharma, A., Gupta, R. & Das, A.. Screening for Various Haemoglobinopathies to Observe Prevalence and Patterns in Population of District Datia (MP), India (2022). https:\/\/doi.org\/10.21203\/rs.3.rs-1245625\/v1","DOI":"10.21203\/rs.3.rs-1245625\/v1"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-06210-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-06210-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-06210-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T11:18:17Z","timestamp":1764587897000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-06210-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,1]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["6210"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-06210-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"type":"electronic","value":"2045-2322"}],"subject":[],"published":{"date-parts":[[2025,12,1]]},"assertion":[{"value":"5 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this article.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The study was approved (or granted exemption) by the appropriate institutional and\/or national research ethics committee (including the name of the ethics committee) via letter no NIRTH\/IE C\/01\/31\/2021 and certify that the study was performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Informed Consent has been taken from patients before sample collection.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"The manuscript has been approved by the Publication Screening Committee of ICMR-NIRTH, Jabalpur and assigned with the number ICMR-NIRTH\/PSC\/31\/2021.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"42835"}}