{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T19:24:08Z","timestamp":1757618648036,"version":"3.44.0"},"reference-count":104,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Agency for Promotion of Science and Technology of Argentina","award":["PICT-2019-00420","PICT-2019-00420","PICT-2019-00420","PICT-2019-00420"],"award-info":[{"award-number":["PICT-2019-00420","PICT-2019-00420","PICT-2019-00420","PICT-2019-00420"]}]},{"name":"Catholic University of C\u00f3rdoba","award":["80020220300055CC","80020220300055CC","80020220300055CC","80020220300055CC"],"award-info":[{"award-number":["80020220300055CC","80020220300055CC","80020220300055CC","80020220300055CC"]}]},{"name":"National Council for Scientific and Technical Research of Argentina","award":["PIP 2017-2019 GI. 11220170100731CO","PIP 2017-2019 GI. 11220170100731CO"],"award-info":[{"award-number":["PIP 2017-2019 GI. 11220170100731CO","PIP 2017-2019 GI. 11220170100731CO"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-07059-6","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T08:57:46Z","timestamp":1752137866000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Induction of systemic and mucosal immune response against Zika virus by vaccination with non-infectious chimeric VLPs"],"prefix":"10.1038","volume":"15","author":[{"given":"Luciana Agostina","family":"Fassola","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda Lara","family":"Rupil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florencia","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Albrieu-Llin\u00e1s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianela del Carmen","family":"Serradell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,10]]},"reference":[{"key":"7059_CR1","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/s41579-021-00639-z","volume":"20","author":"RE Baker","year":"2022","unstructured":"Baker, R. E. et al. Infectious disease in an era of global change. Nat. Rev. Microbiol. 20, 193\u2013205 (2022).","journal-title":"Nat. Rev. Microbiol."},{"key":"7059_CR2","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1038\/s41564-020-0714-0","volume":"5","author":"TC Pierson","year":"2020","unstructured":"Pierson, T. C. & Diamond, M. S. The continued threat of emerging flaviviruses. Nat. Microbiol. 5, 796\u2013812 (2020).","journal-title":"Nat. Microbiol."},{"key":"7059_CR3","doi-asserted-by":"publisher","first-page":"ofaa359","DOI":"10.1093\/ofid\/ofaa359","volume":"7","author":"JP Carrera","year":"2020","unstructured":"Carrera, J. P. et al. Clinical and serological findings of Madariaga and Venezuelan equine encephalitis viral infections: A follow-up study 5 years after an outbreak in Panama. Open. Forum Infect. Dis. 7, ofaa359 (2020).","journal-title":"Open. Forum Infect. Dis."},{"key":"7059_CR4","doi-asserted-by":"publisher","first-page":"50","DOI":"10.15585\/mmwr.mm6902a4","volume":"69","author":"NP Lindsey","year":"2020","unstructured":"Lindsey, N. P., Martin, S. W., Staples, J. E. & Fischer, M. Notes from the field: multistate outbreak of Eastern equine encephalitis Virus \u2014 United states, 2019. Morb Mortal. Wkly. Rep. 69, 50\u201351 (2020).","journal-title":"Morb Mortal. Wkly. Rep."},{"key":"7059_CR5","unstructured":"Encefalitis equina del oeste - Argentina. https:\/\/www.who.int\/es\/emergencies\/disease-outbreak-news\/item\/2023-DON499."},{"key":"7059_CR6","doi-asserted-by":"publisher","DOI":"10.1101\/2024.04.15.24305848","author":"AS Campos","year":"2024","unstructured":"Campos, A. S. et al. Molecular epidemiology of Western equine encephalitis virus in brazil, 2023\u20132024. MedRxiv Prepr Serv. Health Sci.\u00a0(2024). 2024.04.15.24305848.","journal-title":"MedRxiv Prepr Serv. Health Sci."},{"key":"7059_CR7","doi-asserted-by":"crossref","unstructured":"Souza, W. M. et al. Chikungunya: a decade of burden in the Americas. Lancet Reg. Health \u2013 Am 30 (2024).","DOI":"10.1016\/j.lana.2023.100673"},{"key":"7059_CR8","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1002\/jmv.27402","volume":"94","author":"MM Moraes","year":"2022","unstructured":"Moraes, M. M. et al. Detection of saint Louis encephalitis virus in two Brazilian States. J. Med. Virol. 94, 776\u2013781 (2022).","journal-title":"J. Med. Virol."},{"key":"7059_CR9","first-page":"100539","volume":"22","author":"Dengue emergency in","year":"2023","unstructured":"Dengue emergency in. The americas: time for a new continental eradication plan. Lancet Reg. Health - Am. 22, 100539 (2023).","journal-title":"Lancet Reg. Health - Am."},{"key":"7059_CR10","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.virol.2020.01.015","volume":"543","author":"P Pielnaa","year":"2020","unstructured":"Pielnaa, P. et al. Zika virus-spread, epidemiology, genome, transmission cycle, clinical manifestation, associated challenges, vaccine and antiviral drug development. Virology 543, 34\u201342 (2020).","journal-title":"Virology"},{"key":"7059_CR11","doi-asserted-by":"publisher","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","volume":"360","author":"MR Duffy","year":"2009","unstructured":"Duffy, M. R. et al. Zika virus outbreak on Yap island, federated States of Micronesia. N Engl. J. Med. 360, 2536\u20132543 (2009).","journal-title":"N Engl. J. Med."},{"key":"7059_CR12","doi-asserted-by":"publisher","first-page":"e172","DOI":"10.1016\/S1473-3099(17)30446-2","volume":"18","author":"D Musso","year":"2018","unstructured":"Musso, D. et al. Zika virus in French Polynesia 2013\u201314: anatomy of a completed outbreak. Lancet Infect. Dis. 18, e172\u2013e182 (2018).","journal-title":"Lancet Infect. Dis."},{"key":"7059_CR13","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.3201\/eid2006.140138","volume":"20","author":"VM Cao-Lormeau","year":"2014","unstructured":"Cao-Lormeau, V. M. et al. Zika virus, French polynesia, South pacific, 2013. Emerg. Infect. Dis. 20, 1085\u20131086 (2014).","journal-title":"Emerg. Infect. Dis."},{"key":"7059_CR14","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1590\/0074-02760150192","volume":"110","author":"C Zanluca","year":"2015","unstructured":"Zanluca, C. et al. First report of autochthonous transmission of Zika virus in Brazil. Mem. Inst. Oswaldo Cruz. 110, 569\u2013572 (2015).","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"7059_CR15","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1038\/nature22401","volume":"546","author":"NR Faria","year":"2017","unstructured":"Faria, N. R. et al. Establishment and cryptic transmission of Zika virus in Brazil and the Americas. Nature 546, 406\u2013410 (2017).","journal-title":"Nature"},{"key":"7059_CR16","unstructured":"WHO Director-General summarizes the outcome of the Emergency Committee. regarding clusters of microcephaly and Guillain-Barr\u00e9 syndrome. https:\/\/www.who.int\/news\/item\/01-02-2016-who-director-general-summarizes-the-outcome-of-the-emergency-committee-regarding-clusters-of-microcephaly-and-guillain-barr\u00e9-syndrome."},{"key":"7059_CR17","doi-asserted-by":"publisher","first-page":"296","DOI":"10.3390\/v12030296","volume":"12","author":"GA Durand","year":"2020","unstructured":"Durand, G. A. et al. Vector-Borne transmission of the Zika virus Asian genotype in Europe. Viruses 12, 296 (2020).","journal-title":"Viruses"},{"key":"7059_CR18","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1186\/s12976-016-0046-1","volume":"13","author":"L Dinh","year":"2016","unstructured":"Dinh, L., Chowell, G., Mizumoto, K. & Nishiura, H. Estimating the subcritical transmissibility of the Zika outbreak in the state of florida, USA, 2016. Theor. Biol. Med. Model. 13, 20 (2016).","journal-title":"Theor. Biol. Med. Model."},{"key":"7059_CR19","doi-asserted-by":"crossref","unstructured":"Wilder-Smith, A., Chang, C. R. & Leong, W. Y. Zika in travellers 1947\u20132017: a systematic review. J. Travel Med. 25, (2018).","DOI":"10.1093\/jtm\/tay044"},{"key":"7059_CR20","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1038\/s41564-019-0376-y","volume":"4","author":"MUG Kraemer","year":"2019","unstructured":"Kraemer, M. U. G. et al. Past and future spread of the arbovirus vectors Aedes aegypti and Aedes albopictus. Nat. Microbiol. 4, 854\u2013863 (2019).","journal-title":"Nat. Microbiol."},{"key":"7059_CR21","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1097\/JS9.0000000000000182","volume":"109","author":"R Sah","year":"2023","unstructured":"Sah, R., Mohanty, A., Paul, D. & Padhi, B. K. Recent outbreak of Zika virus in India amid ongoing COVID-19 and Monkeypox outbreak: A call for action. Int. J. Surg. Lond. Engl. 109, 601\u2013603 (2023).","journal-title":"Int. J. Surg. Lond. Engl."},{"key":"7059_CR22","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1111\/gcb.15384","volume":"27","author":"SJ Ryan","year":"2021","unstructured":"Ryan, S. J. et al. Warming temperatures could expose more than 1.3 billion new people to Zika virus risk by 2050. Glob Change Biol. 27, 84\u201393 (2021).","journal-title":"Glob Change Biol."},{"key":"7059_CR23","doi-asserted-by":"publisher","first-page":"1356","DOI":"10.1016\/S1473-3099(16)30318-8","volume":"16","author":"TVB Ara\u00fajo","year":"2016","unstructured":"Ara\u00fajo, T. V. B. et al. Association between Zika virus infection and microcephaly in brazil, January to may, 2016: preliminary report of a case-control study. Lancet Infect. Dis. 16, 1356\u20131363 (2016).","journal-title":"Lancet Infect. Dis."},{"key":"7059_CR24","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1001\/jamaophthalmol.2016.0267","volume":"134","author":"P de Freitas","year":"2016","unstructured":"de Freitas, P. Ocular findings in infants with microcephaly associated with presumed Zika virus congenital infection in salvador, Brazil. JAMA Ophthalmol. 134, 529\u2013535 (2016).","journal-title":"JAMA Ophthalmol."},{"key":"7059_CR25","doi-asserted-by":"publisher","first-page":"2321","DOI":"10.1056\/NEJMoa1602412","volume":"375","author":"P Brasil","year":"2016","unstructured":"Brasil, P. et al. Zika virus infection in pregnant women in Rio de Janeiro. N Engl. J. Med. 375, 2321\u20132334 (2016).","journal-title":"N Engl. J. Med."},{"key":"7059_CR26","doi-asserted-by":"publisher","first-page":"1190","DOI":"10.1001\/jamaneurol.2017.1703","volume":"74","author":"IRF da Silva","year":"2017","unstructured":"da Silva, I. R. F., Frontera, J. A., Bispo de Filippis, A. M., do Nascimento, O. J. M. & for the RIO-GBS-ZIKV Research Group. Neurologic complications associated with the Zika virus in Brazilian adults. JAMA Neurol. 74, 1190\u20131198 (2017).","journal-title":"JAMA Neurol."},{"key":"7059_CR27","doi-asserted-by":"publisher","first-page":"20720","DOI":"10.2807\/1560-7917.ES2014.19.9.20720","volume":"19","author":"E Oehler","year":"2014","unstructured":"Oehler, E. et al. Zika virus infection complicated by Guillain-Barr\u00e9 syndrome \u2013 case report, French polynesia, December 2013. Eurosurveillance 19, 20720 (2014).","journal-title":"Eurosurveillance"},{"key":"7059_CR28","doi-asserted-by":"publisher","first-page":"359","DOI":"10.3201\/eid2102.141363","volume":"21","author":"D Musso","year":"2015","unstructured":"Musso, D. et al. Potential sexual transmission of Zika virus. Emerg. Infect. Dis. 21, 359\u2013361 (2015).","journal-title":"Emerg. Infect. Dis."},{"key":"7059_CR29","first-page":"30159","volume":"21","author":"L Barzon","year":"2016","unstructured":"Barzon, L. et al. Isolation of infectious Zika virus from saliva and prolonged viral RNA shedding in a traveller returning from the Dominican Republic to italy, January 2016. Eurosurveillance 21, 30159 (2016).","journal-title":"Eurosurveillance"},{"key":"7059_CR30","doi-asserted-by":"publisher","first-page":"880","DOI":"10.3201\/eid1705.101939","volume":"17","author":"BD Foy","year":"2011","unstructured":"Foy, B. D. et al. Probable non-vector-borne transmission of Zika virus, colorado, USA. Emerg. Infect. Dis. 17, 880\u2013882 (2011).","journal-title":"Emerg. Infect. Dis."},{"key":"7059_CR31","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.htct.2018.01.011","volume":"40","author":"MM Magnus","year":"2018","unstructured":"Magnus, M. M. et al. Risk of Zika virus transmission by blood donations in Brazil. Hematol. Transfus. Cell. Ther. 40, 250\u2013254 (2018).","journal-title":"Hematol. Transfus. Cell. Ther."},{"key":"7059_CR32","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1126\/science.aaf5316","volume":"352","author":"D Sirohi","year":"2016","unstructured":"Sirohi, D. et al. The 3.8 \u00c5 resolution cryo-EM structure of Zika virus. Science 352, 467\u2013470 (2016).","journal-title":"Science"},{"key":"7059_CR33","doi-asserted-by":"publisher","first-page":"559","DOI":"10.3390\/v10100559","volume":"10","author":"G Sager","year":"2018","unstructured":"Sager, G., Gabaglio, S., Sztul, E. & Belov, G. A. Role of host cell secretory machinery in Zika virus life cycle. Viruses 10, 559 (2018).","journal-title":"Viruses"},{"key":"7059_CR34","doi-asserted-by":"publisher","first-page":"S935","DOI":"10.1093\/infdis\/jix515","volume":"216","author":"D Sirohi","year":"2017","unstructured":"Sirohi, D. & Kuhn, R. J. Zika virus structure, maturation, and receptors. J. Infect. Dis. 216, S935\u2013S944 (2017).","journal-title":"J. Infect. Dis."},{"key":"7059_CR35","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1016\/j.cell.2016.07.020","volume":"166","author":"H Zhao","year":"2016","unstructured":"Zhao, H. et al. Structural basis of Zika virus-specific antibody protection. Cell 166, 1016\u20131027 (2016).","journal-title":"Cell"},{"key":"7059_CR36","doi-asserted-by":"publisher","first-page":"14722","DOI":"10.1038\/ncomms14722","volume":"8","author":"SS Hasan","year":"2017","unstructured":"Hasan, S. S. et al. A human antibody against Zika virus crosslinks the E protein to prevent infection. Nat. Commun. 8, 14722 (2017).","journal-title":"Nat. Commun."},{"key":"7059_CR37","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.coi.2018.04.024","volume":"53","author":"JM Richner","year":"2018","unstructured":"Richner, J. M. & Diamond, M. S. Zika virus vaccines: immune response, current status, and future challenges. Curr. Opin. Immunol. 53, 130\u2013136 (2018).","journal-title":"Curr. Opin. Immunol."},{"key":"7059_CR38","doi-asserted-by":"publisher","first-page":"aaf8160","DOI":"10.1126\/science.aaf8160","volume":"353","author":"J Lessler","year":"2016","unstructured":"Lessler, J. et al. Assessing the global threat from Zika virus. Science 353, aaf8160 (2016).","journal-title":"Science"},{"key":"7059_CR39","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1128\/am.25.4.539-544.1973","volume":"25","author":"RA Mason","year":"1973","unstructured":"Mason, R. A., Tauraso, N. M., Spertzel, R. O. & Ginn, R. K. Yellow fever vaccine: direct challenge of monkeys given graded doses of 17D vaccine. Appl. Microbiol. 25, 539\u2013544 (1973).","journal-title":"Appl. Microbiol."},{"key":"7059_CR40","doi-asserted-by":"publisher","first-page":"e257","DOI":"10.1016\/S2214-109X(23)00522-3","volume":"12","author":"V Tricou","year":"2024","unstructured":"Tricou, V. et al. Long-term efficacy and safety of a tetravalent dengue vaccine (TAK-003): 4\u00b75-year results from a phase 3, randomised, double-blind, placebo-controlled trial. Lancet Glob Health. 12, e257\u2013e270 (2024).","journal-title":"Lancet Glob Health"},{"key":"7059_CR41","doi-asserted-by":"publisher","first-page":"e0005608","DOI":"10.1371\/journal.pntd.0005608","volume":"11","author":"H Boigard","year":"2017","unstructured":"Boigard, H. et al. Zika virus-like particle (VLP) based vaccine. PLoS Negl. Trop. Dis. 11, e0005608 (2017).","journal-title":"PLoS Negl. Trop. Dis."},{"key":"7059_CR42","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.biotechadv.2017.09.004","volume":"36","author":"HH Lin","year":"2018","unstructured":"Lin, H. H., Yip, B. S., Huang, L. M. & Wu, S. C. Zika virus structural biology and progress in vaccine development. Biotechnol. Adv. 36, 47\u201353 (2018).","journal-title":"Biotechnol. Adv."},{"key":"7059_CR43","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.3390\/v15051109","volume":"15","author":"M Kheirvari","year":"2023","unstructured":"Kheirvari, M., Liu, H. & Tumban, E. Virus-like particle vaccines and platforms for vaccine development. Viruses 15, 1109 (2023).","journal-title":"Viruses"},{"key":"7059_CR44","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1016\/S1473-3099(23)00192-5","volume":"23","author":"MA Koren","year":"2023","unstructured":"Koren, M. A. et al. Safety and immunogenicity of a purified inactivated Zika virus vaccine candidate in adults primed with a Japanese encephalitis virus or yellow fever virus vaccine in the USA: a phase 1, randomised, double-blind, placebo-controlled clinical trial. Lancet Infect. Dis. 23, 1175\u20131185 (2023).","journal-title":"Lancet Infect. Dis."},{"key":"7059_CR45","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1016\/S1473-3099(20)30085-2","volume":"20","author":"KE Stephenson","year":"2020","unstructured":"Stephenson, K. E. et al. Safety and immunogenicity of a Zika purified inactivated virus vaccine given via standard, accelerated, or shortened schedules: a single-centre, double-blind, sequential-group, randomised, placebo-controlled, phase 1 trial. Lancet Infect. Dis. 20, 1061\u20131070 (2020).","journal-title":"Lancet Infect. Dis."},{"key":"7059_CR46","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/S1473-3099(22)00764-2","volume":"23","author":"B Essink","year":"2023","unstructured":"Essink, B. et al. The safety and immunogenicity of two Zika virus mRNA vaccine candidates in healthy flavivirus baseline seropositive and seronegative adults: the results of two randomised, placebo-controlled, dose-ranging, phase 1 clinical trials. Lancet Infect. Dis. 23, 621\u2013633 (2023).","journal-title":"Lancet Infect. Dis."},{"key":"7059_CR47","doi-asserted-by":"publisher","first-page":"2524","DOI":"10.1016\/j.vaccine.2023.02.055","volume":"41","author":"A Bacon","year":"2023","unstructured":"Bacon, A. et al. Generation of a thermostable, oral Zika vaccine that protects against virus challenge in non-human primates. Vaccine 41, 2524\u20132533 (2023).","journal-title":"Vaccine"},{"key":"7059_CR48","doi-asserted-by":"publisher","first-page":"751883","DOI":"10.3389\/fimmu.2021.751883","volume":"12","author":"MS Hsieh","year":"2021","unstructured":"Hsieh, M. S. et al. Intranasal vaccination with recombinant antigen-FLIPr fusion protein alone induces long-lasting systemic antibody responses and broad T cell responses. Front. Immunol. 12, 751883 (2021).","journal-title":"Front. Immunol."},{"key":"7059_CR49","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1038\/s41467-018-08265-9","volume":"10","author":"MC Serradell","year":"2019","unstructured":"Serradell, M. C. et al. Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins. Nat. Commun. 10, 361 (2019).","journal-title":"Nat. Commun."},{"key":"7059_CR50","doi-asserted-by":"publisher","first-page":"1369","DOI":"10.1098\/rstb.1997.0122","volume":"352","author":"TE Nash","year":"1997","unstructured":"Nash, T. E. Antigenic variation in Giardia lamblia and the host\u2019s immune response. Philos. Trans. R Soc. Lond. B Biol. Sci. 352, 1369\u20131375 (1997).","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"key":"7059_CR51","doi-asserted-by":"crossref","unstructured":"Haga, K., Chen, Z., Himeno, M., Majima, R. & Moi, M. L. (eds) (Nancy), Utility of an in-vitro micro-neutralizing test in comparison to a plaque reduction neutralization test for dengue virus, japanese encephalitis virus, and zika virus serology and drug screening. Pathogens. 13, 8 (2024).","DOI":"10.3390\/pathogens13010008"},{"key":"7059_CR52","doi-asserted-by":"publisher","first-page":"e0006210","DOI":"10.1371\/journal.pntd.0006210","volume":"12","author":"MA Salvo","year":"2018","unstructured":"Salvo, M. A., Kingstad-Bakke, B., Salas-Quinchucua, C., Camacho, E. & Osorio, J. E. Zika virus like particles elicit protective antibodies in mice. PLoS Negl. Trop. Dis. 12, e0006210 (2018).","journal-title":"PLoS Negl. Trop. Dis."},{"key":"7059_CR53","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1056\/NEJMoa1600651","volume":"374","author":"J Mlakar","year":"2016","unstructured":"Mlakar, J. et al. Zika virus associated with microcephaly. N Engl. J. Med. 374, 951\u2013958 (2016).","journal-title":"N Engl. J. Med."},{"key":"7059_CR54","doi-asserted-by":"publisher","first-page":"e0005869","DOI":"10.1371\/journal.pntd.0005869","volume":"11","author":"AR Styczynski","year":"2017","unstructured":"Styczynski, A. R. et al. Increased rates of Guillain-Barr\u00e9 syndrome associated with Zika virus outbreak in the Salvador metropolitan area, Brazil. PLoS Negl. Trop. Dis. 11, e0005869 (2017).","journal-title":"PLoS Negl. Trop. Dis."},{"key":"7059_CR55","doi-asserted-by":"publisher","first-page":"1303","DOI":"10.1093\/infdis\/jiac482","volume":"227","author":"CJ Acosta","year":"2023","unstructured":"Acosta, C. J. et al. Persistence of immunogenicity of a purified inactivated Zika virus vaccine candidate in healthy adults: 2 years of follow-up compared with natural infection. J. Infect. Dis. 227, 1303\u20131312 (2023).","journal-title":"J. Infect. Dis."},{"key":"7059_CR56","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1186\/1743-422X-10-129","volume":"10","author":"AJ Chua","year":"2013","unstructured":"Chua, A. J. et al. A novel platform for virus-like particle-display of flaviviral envelope domain III: induction of dengue and West nile virus neutralizing antibodies. Virol. J. 10, 129 (2013).","journal-title":"Virol. J."},{"key":"7059_CR57","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/S0042-6822(03)00485-9","volume":"314","author":"CS Adamson","year":"2003","unstructured":"Adamson, C. S. et al. A block in virus-like particle maturation following assembly of murine leukaemia virus in insect cells. Virology 314, 488\u2013496 (2003).","journal-title":"Virology"},{"key":"7059_CR58","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.vaccine.2023.11.048","volume":"42","author":"AB Gashti","year":"2024","unstructured":"Gashti, A. B. et al. Production, purification and immunogenicity of gag virus-like particles carrying SARS-CoV-2 components. Vaccine 42, 40\u201352 (2024).","journal-title":"Vaccine"},{"key":"7059_CR59","doi-asserted-by":"publisher","first-page":"13463","DOI":"10.1128\/JVI.79.21.13463-13472.2005","volume":"79","author":"D Ako-Adjei","year":"2005","unstructured":"Ako-Adjei, D., Johnson, M. C. & Vogt, V. M. The retroviral capsid domain dictates virion size, morphology, and coassembly of gag into Virus-Like particles. J. Virol. 79, 13463 (2005).","journal-title":"J. Virol."},{"key":"7059_CR60","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1038\/nature18952","volume":"536","author":"RA Larocca","year":"2016","unstructured":"Larocca, R. A. et al. Vaccine protection against Zika virus from Brazil. Nature 536, 474\u2013478 (2016).","journal-title":"Nature"},{"key":"7059_CR61","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1038\/nature21428","volume":"543","author":"N Pardi","year":"2017","unstructured":"Pardi, N. et al. Zika virus protection by a single low dose nucleoside modified mRNA vaccination. Nature 543, 248\u2013251 (2017).","journal-title":"Nature"},{"key":"7059_CR62","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.3390\/pharmaceutics14051014","volume":"14","author":"CH Chen","year":"2022","unstructured":"Chen, C. H. et al. Intranasal immunization with Zika virus envelope domain III-Flagellin fusion protein elicits systemic and mucosal immune responses and protection against subcutaneous and intravaginal virus challenges. Pharmaceutics 14, 1014 (2022).","journal-title":"Pharmaceutics"},{"key":"7059_CR63","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1146\/annurev.iy.07.040189.001045","volume":"7","author":"TR Mosmann","year":"1989","unstructured":"Mosmann, T. R. & Coffman, R. L. TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Annu. Rev. Immunol. 7, 145\u2013173 (1989).","journal-title":"Annu. Rev. Immunol."},{"key":"7059_CR64","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/334255a0","volume":"334","author":"TL Stevens","year":"1988","unstructured":"Stevens, T. L. et al. Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature 334, 255\u2013258 (1988).","journal-title":"Nature"},{"key":"7059_CR65","doi-asserted-by":"publisher","first-page":"4287","DOI":"10.1016\/j.vaccine.2017.04.052","volume":"35","author":"M Yang","year":"2017","unstructured":"Yang, M., Dent, M., Lai, H., Sun, H. & Chen, Q. Immunization of Zika virus envelope protein domain III induces specific and neutralizing immune responses against Zika virus. Vaccine 35, 4287\u20134294 (2017).","journal-title":"Vaccine"},{"key":"7059_CR66","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.coviro.2020.07.008","volume":"43","author":"LA Sanchez Vargas","year":"2020","unstructured":"Sanchez Vargas, L. A., Mathew, A. & Rothman, L. A. T lymphocyte responses to flaviviruses \u2014 diverse cell populations affect tendency toward protection and disease. Curr. Opin. Virol. 43, 28\u201334 (2020).","journal-title":"Curr. Opin. Virol."},{"key":"7059_CR67","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1016\/j.it.2017.05.004","volume":"38","author":"NS Lima","year":"2017","unstructured":"Lima, N. S., Rolland, M., Modjarrad, K. & Trautmann, L. T. Cell immunity and Zika virus vaccine development. Trends Immunol. 38, 594\u2013605 (2017).","journal-title":"Trends Immunol."},{"key":"7059_CR68","doi-asserted-by":"crossref","unstructured":"Huang, H. et al. CD8\u207a T cell immune response in immunocompetent mice during Zika virus infection. J. Virol. 91, e01028\u201317 (2017).","DOI":"10.1128\/JVI.00900-17"},{"key":"7059_CR69","doi-asserted-by":"crossref","unstructured":"Weiskopf, D. & Sette, A. T-Cell immunity to infection with dengue virus in humans. Front. Immunol. 5, (2014).","DOI":"10.3389\/fimmu.2014.00093"},{"key":"7059_CR70","doi-asserted-by":"publisher","first-page":"77","DOI":"10.3390\/v9040077","volume":"9","author":"AM Watson","year":"2017","unstructured":"Watson, A. M. & Klimstra, W. B. T cell-mediated immunity towards yellow fever virus and useful animal models. Viruses 9, 77 (2017).","journal-title":"Viruses"},{"key":"7059_CR71","doi-asserted-by":"publisher","first-page":"e81953","DOI":"10.1371\/journal.pone.0081953","volume":"8","author":"PCC Neves","year":"2013","unstructured":"Neves, P. C. C., Santos, J. R., Tubar\u00e3o, L. N., Bonaldo, M. C. & Galler, R. Early IFN-gamma production after YF 17D vaccine virus immunization in mice and its association with adaptive immune responses. PLoS ONE. 8, e81953 (2013).","journal-title":"PLOS ONE"},{"key":"7059_CR72","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41541-017-0039-z","volume":"3","author":"LKM Lam","year":"2018","unstructured":"Lam, L. K. M., Watson, A. M., Ryman, K. D. & Klimstra, W. B. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. Npj Vaccines. 3, 1\u201310 (2018).","journal-title":"Npj Vaccines"},{"key":"7059_CR73","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.chom.2016.12.010","volume":"21","author":"A Elong Ngono","year":"2017","unstructured":"Elong Ngono, A. et al. Mapping and role of the CD8\u2009+\u2009T cell response during primary Zika virus infection in mice. Cell. Host Microbe. 21, 35\u201346 (2017).","journal-title":"Cell. Host Microbe"},{"key":"7059_CR74","doi-asserted-by":"crossref","unstructured":"Hassert, M., Harris, M. G., Brien, J. D. & Pinto, A. K. Identification of protective CD8 T cell responses in a mouse model of Zika virus infection. Front. Immunol. 10, (2019).","DOI":"10.3389\/fimmu.2019.01678"},{"key":"7059_CR75","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/s41541-021-00282-y","volume":"6","author":"WX Chin","year":"2021","unstructured":"Chin, W. X. et al. A single-dose live attenuated chimeric vaccine candidate against Zika virus. NPJ Vaccines. 6, 20 (2021).","journal-title":"NPJ Vaccines"},{"key":"7059_CR76","doi-asserted-by":"publisher","first-page":"1464","DOI":"10.3390\/vaccines9121464","volume":"9","author":"LG Medina-Mag\u00fces","year":"2021","unstructured":"Medina-Mag\u00fces, L. G. et al. mRNA vaccine protects against Zika virus. Vaccines 9, 1464 (2021).","journal-title":"Vaccines"},{"key":"7059_CR77","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.it.2017.01.006","volume":"38","author":"N Amatya","year":"2017","unstructured":"Amatya, N., Garg, A. V. & Gaffen, S. L. IL-17 signaling: the Yin and the Yang. Trends Immunol. 38, 310\u2013322 (2017).","journal-title":"Trends Immunol."},{"key":"7059_CR78","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1016\/j.immuni.2019.03.021","volume":"50","author":"MJ McGeachy","year":"2019","unstructured":"McGeachy, M. J., Cua, D. J. & Gaffen, S. L. The IL-17 family of cytokines in health and disease. Immunity 50, 892\u2013906 (2019).","journal-title":"Immunity"},{"key":"7059_CR79","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1007\/s10875-012-9855-0","volume":"33","author":"A Jain","year":"2013","unstructured":"Jain, A., Pandey, N., Garg, R. K. & Kumar, R. IL-17 level in patients with dengue virus infection & its association with severity of illness. J. Clin. Immunol. 33, 613\u2013618 (2013).","journal-title":"J. Clin. Immunol."},{"key":"7059_CR80","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1186\/1471-2334-10-356","volume":"10","author":"P Becquart","year":"2010","unstructured":"Becquart, P. et al. Acute dengue virus 2 infection in Gabonese patients is associated with an early innate immune response, including strong interferon alpha production. BMC Infect. Dis. 10, 356 (2010).","journal-title":"BMC Infect. Dis."},{"key":"7059_CR81","first-page":"e01529","volume":"91","author":"D Acharya","year":"2016","unstructured":"Acharya, D. et al. Interleukin-17A promotes CD8\u2009+\u2009T cell cytotoxicity to facilitate West nile virus clearance. J. Virol. 91, e01529\u2013e01516 (2016).","journal-title":"J. Virol."},{"key":"7059_CR82","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.ijid.2020.02.014","volume":"94","author":"J Zu\u00f1iga","year":"2020","unstructured":"Zu\u00f1iga, J. et al. A unique immune signature of serum cytokine and chemokine dynamics in patients with Zika virus infection from a tropical region in Southern Mexico. Int. J. Infect. Dis. 94, 4\u201311 (2020).","journal-title":"Int. J. Infect. Dis."},{"key":"7059_CR83","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-017-17765-5","volume":"8","author":"RSS Azevedo","year":"2018","unstructured":"Azevedo, R. S. S. et al. In situ immune response and mechanisms of cell damage in central nervous system of fatal cases microcephaly by Zika virus. Sci. Rep. 8, 1 (2018).","journal-title":"Sci. Rep."},{"key":"7059_CR84","doi-asserted-by":"publisher","first-page":"16018","DOI":"10.1038\/npjvaccines.2016.18","volume":"1","author":"MC Serradell","year":"2016","unstructured":"Serradell, M. C. et al. Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans. NPJ Vaccines. 1, 16018 (2016).","journal-title":"NPJ Vaccines"},{"key":"7059_CR85","doi-asserted-by":"publisher","first-page":"128","DOI":"10.3390\/vaccines12020128","volume":"12","author":"EL Stewart","year":"2024","unstructured":"Stewart, E. L. et al. Lung IL-17A-producing CD4\u2009+\u2009T cells correlate with protection after intrapulmonary vaccination with differentially adjuvanted tuberculosis vaccines. Vaccines 12, 128 (2024).","journal-title":"Vaccines"},{"key":"7059_CR86","doi-asserted-by":"publisher","first-page":"102540","DOI":"10.1016\/j.tube.2024.102540","volume":"148","author":"SC Derrick","year":"2024","unstructured":"Derrick, S. C., Yang, A. & Cowley, S. Enhanced efficacy of BCG vaccine formulated in adjuvant is dependent on IL-17A expression. Tuberculosis 148, 102540 (2024).","journal-title":"Tuberculosis"},{"key":"7059_CR87","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1038\/mi.2017.110","volume":"11","author":"RHM Raeven","year":"2018","unstructured":"Raeven, R. H. M. et al. Molecular and cellular signatures underlying superior immunity against Bordetella pertussis upon pulmonary vaccination. Mucosal Immunol. 11, 1009 (2018).","journal-title":"Mucosal Immunol."},{"key":"7059_CR88","doi-asserted-by":"publisher","first-page":"1753","DOI":"10.1038\/s41385-018-0073-9","volume":"11","author":"L Solans","year":"2018","unstructured":"Solans, L. et al. IL-17-dependent SIgA-mediated protection against nasal Bordetella pertussis infection by live attenuated BPZE1 vaccine. Mucosal Immunol. 11, 1753\u20131762 (2018).","journal-title":"Mucosal Immunol."},{"key":"7059_CR89","doi-asserted-by":"crossref","unstructured":"Kimura, A., Naka, T. & Kishimoto, T. IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells. Proc. Natl. Acad. Sci. 104, 12099\u201312104 (2007).","DOI":"10.1073\/pnas.0705268104"},{"key":"7059_CR90","doi-asserted-by":"publisher","first-page":"673","DOI":"10.4269\/ajtmh.2008.79.673","volume":"79","author":"BN Restrepo","year":"2008","unstructured":"Restrepo, B. N. et al. Serum levels of cytokines in two ethnic groups with dengue virus infection. Am. J. Trop. Med. Hyg. 79, 673\u2013677 (2008).","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"7059_CR91","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.3390\/v15061320","volume":"15","author":"EMS Fialho","year":"2023","unstructured":"Fialho, E. M. S. et al. Maternal Th17 profile after Zika virus infection is involved in congenital Zika syndrome development in children. Viruses 15, 1320 (2023).","journal-title":"Viruses"},{"key":"7059_CR92","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.epidem.2017.06.003","volume":"21","author":"O Maxian","year":"2017","unstructured":"Maxian, O., Neufeld, A., Talis, E. J., Childs, L. M. & Blackwood, J. C. Zika virus dynamics: when does sexual transmission matter? Epidemics 21, 48\u201355 (2017).","journal-title":"Epidemics"},{"key":"7059_CR93","doi-asserted-by":"publisher","first-page":"28070","DOI":"10.1038\/srep28070","volume":"6","author":"D Gao","year":"2016","unstructured":"Gao, D. et al. Prevention and control of Zika as a Mosquito-Borne and sexually transmitted disease: A mathematical modeling analysis. Sci. Rep. 6, 28070 (2016).","journal-title":"Sci. Rep."},{"key":"7059_CR94","doi-asserted-by":"publisher","first-page":"e00038","DOI":"10.1128\/JVI.00038-18","volume":"92","author":"JM Scott","year":"2018","unstructured":"Scott, J. M. et al. Cellular and humoral immunity protect against vaginal Zika virus infection in mice. J. Virol. 92, e00038\u2013e00018 (2018).","journal-title":"J. Virol."},{"key":"7059_CR95","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/j.coi.2012.05.006","volume":"24","author":"Y Kumamoto","year":"2012","unstructured":"Kumamoto, Y. & Iwasaki, A. Unique features of antiviral immune system of the vaginal mucosa. Curr. Opin. Immunol. 24, 411\u2013416 (2012).","journal-title":"Curr. Opin. Immunol."},{"key":"7059_CR96","doi-asserted-by":"publisher","first-page":"e0010588","DOI":"10.1371\/journal.pntd.0010588","volume":"16","author":"D Thompson","year":"2022","unstructured":"Thompson, D. et al. Zika virus-like particle vaccine fusion loop mutation increases production yield but fails to protect AG129 mice against Zika virus challenge. PLoS Negl. Trop. Dis. 16, e0010588 (2022).","journal-title":"PLoS Negl. Trop. Dis."},{"key":"7059_CR97","doi-asserted-by":"publisher","first-page":"603","DOI":"10.3390\/vaccines8040603","volume":"8","author":"BS Berneck","year":"2020","unstructured":"Berneck, B. S., Rockstroh, A., Fertey, J., Grunwald, T. & Ulbert, S. A recombinant Zika virus envelope protein with mutations in the conserved fusion loop leads to reduced antibody cross-reactivity upon vaccination. Vaccines 8, 603 (2020).","journal-title":"Vaccines"},{"key":"7059_CR98","doi-asserted-by":"crossref","unstructured":"Urakami, A. et al. An envelope-modified tetravalent dengue virus-like-particle vaccine has implications for flavivirus vaccine design. J. Virol. 91, e01181\u201317 (2017).","DOI":"10.1128\/JVI.01181-17"},{"key":"7059_CR99","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1016\/j.vaccine.2018.12.040","volume":"37","author":"KS Vannice","year":"2019","unstructured":"Vannice, K. S. et al. Demonstrating vaccine effectiveness during a waning epidemic: A WHO\/NIH meeting report on approaches to development and licensure of Zika vaccine candidates. Vaccine 37, 863\u2013868 (2019).","journal-title":"Vaccine"},{"key":"7059_CR100","doi-asserted-by":"publisher","first-page":"46375","DOI":"10.1038\/srep46375","volume":"7","author":"K Sumathy","year":"2017","unstructured":"Sumathy, K. et al. Protective efficacy of Zika vaccine in AG129 mouse model. Sci. Rep. 7, 46375 (2017).","journal-title":"Sci. Rep."},{"key":"7059_CR101","doi-asserted-by":"publisher","first-page":"e0009195","DOI":"10.1371\/journal.pntd.0009195","volume":"15","author":"L Vang","year":"2021","unstructured":"Vang, L. et al. Zika virus-like particle vaccine protects AG129 mice and rhesus macaques against Zika virus. PLoS Negl. Trop. Dis. 15, e0009195 (2021).","journal-title":"PLoS Negl. Trop. Dis."},{"key":"7059_CR102","doi-asserted-by":"publisher","first-page":"e35","DOI":"10.1056\/NEJMoa1708120","volume":"385","author":"P Tebas","year":"2021","unstructured":"Tebas, P. et al. ZIKA-001: safety and immunogenicity of an engineered DNA vaccine against Zika virus infection. N Engl. J. Med. 385, e35 (2021).","journal-title":"N Engl. J. Med."},{"key":"7059_CR103","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.1038\/s41590-019-0477-z","volume":"20","author":"JL Slon-Campos","year":"2019","unstructured":"Slon-Campos, J. L. et al. A protective Zika virus E-dimer-based subunit vaccine engineered to abrogate antibody-dependent enhancement of dengue infection. Nat. Immunol. 20, 1291\u20131298 (2019).","journal-title":"Nat. Immunol."},{"key":"7059_CR104","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1007\/978-1-0716-1884-4_26","volume-title":"Vaccine Design: Methods and Protocols, Volume 1. Vaccines for Human Diseases","author":"LL Rupil","year":"2022","unstructured":"Rupil, L. L., del Carmen Serradell, M. & Luj\u00e1n, H. D. Production of oral vaccines based on virus-Like particles pseudotyped with protozoan-surface proteins. In Vaccine Design: Methods and Protocols, Volume 1. Vaccines for Human Diseases (ed Thomas, S.) 503\u2013537. (Springer US, 2022)."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07059-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07059-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07059-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T05:35:24Z","timestamp":1757223324000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07059-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,10]]},"references-count":104,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["7059"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-07059-6","relation":{},"ISSN":["2045-2322"],"issn-type":[{"type":"electronic","value":"2045-2322"}],"subject":[],"published":{"date-parts":[[2025,7,10]]},"assertion":[{"value":"10 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This research strictly followed the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The well-being of all animals and the management of animal facilities were supervised by CICUAL-UCC. The study protocol was approved by CICUAL-UCC, guaranteeing adherence to ethical guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics statement"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"24834"}}