{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:47:00Z","timestamp":1790639220357,"version":"4.1.0"},"reference-count":55,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Shanghai Pujiang Program","award":["24PJD081"],"award-info":[{"award-number":["24PJD081"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>This study investigates the protective effects of <jats:italic>Lactiplantibacillus plantarum<\/jats:italic> Lp05 on ethanol-induced liver injury in zebrafish (<jats:italic>Danio rerio<\/jats:italic>), focusing on its mechanisms related to metabolic pathway regulation and gut microbiota composition. We administered Lp05 to zebrafish exposed to ethanol to assess its impact on liver enzymes and overall liver health. Key metrics included the expression levels of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and changes in gut microbiota composition through 16S rRNA sequencing. Metabolomic analyses were conducted to identify affected metabolic pathways. Treatment with Lp05 significantly increased ADH and ALDH expression, enhancing alcohol metabolism and reducing ALT and AST activities, thereby mitigating liver cell damage. Metabolomic analysis revealed significant modulation of biotin and glycerolipid metabolism pathways, crucial for reducing liver injury. Lp05 also altered gut microbiota, increasing beneficial bacteria such as <jats:italic>Fluviicola<\/jats:italic> and <jats:italic>Delftia<\/jats:italic>, while decreasing pathogenic bacteria like <jats:italic>Acinetobacter<\/jats:italic> and <jats:italic>Aeromonas<\/jats:italic>. This bacterial modulation contributed to phenylalanine metabolism regulation, which alleviated intestinal inflammation and liver injury. Additionally, Lp05 promoted the degradation of polycyclic aromatic hydrocarbons (PAHs), reducing their health risks. Lp05 exhibits potential therapeutic effects against ethanol-induced liver injury in zebrafish by enhancing alcohol metabolism, modulating metabolic pathways, and altering gut microbiota. These findings suggest that Lp05 may offer a novel preventative and therapeutic strategy for managing alcoholic liver injury. Alcohol-induced liver injury is a significant global health concern with limited effective therapeutic options. This study highlights the potential of <jats:italic>Lactiplantibacillus plantarum<\/jats:italic> Lp05 as a novel probiotic intervention for mitigating ethanol-induced liver damage. By enhancing alcohol metabolism, regulating critical metabolic pathways such as biotin and glycerolipid metabolism, and modulating gut microbiota composition, Lp05 addresses both the metabolic and microbiological aspects of liver health. The promotion of beneficial bacteria and the suppression of pathogenic strains further contribute to alleviating liver injury and systemic inflammation. These findings underscore the therapeutic promise of Lp05 in managing alcoholic liver injury and provide a foundation for its development as a preventative and therapeutic strategy in human applications.<\/jats:p>","DOI":"10.1038\/s41598-025-07111-5","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T23:21:04Z","timestamp":1751412064000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Lactiplantibacillus plantarum Lp05 protects against ethanol-induced liver injury in zebrafish through metabolic and microbiota modulation"],"prefix":"10.1038","volume":"15","author":[{"given":"Yixuan","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghui","family":"Gai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"key":"7111_CR1","doi-asserted-by":"publisher","first-page":"688780","DOI":"10.3389\/fphys.2021.688780","volume":"12","author":"T Chen","year":"2021","unstructured":"Chen, T., Li, R. & Chen, P. Gut microbiota and chemical-induced acute liver injury. Front. Physiol. 12, 688780 (2021).","journal-title":"Front. Physiol."},{"key":"7111_CR2","doi-asserted-by":"publisher","first-page":"e4","DOI":"10.1017\/S1462399411002110","volume":"14","author":"X Gu","year":"2012","unstructured":"Gu, X. & Manautou, J. E. Molecular mechanisms underlying chemical liver injury. Expert Rev. Mol. Med. 14, e4 (2012).","journal-title":"Expert Rev. Mol. Med."},{"key":"7111_CR3","doi-asserted-by":"publisher","first-page":"193","DOI":"10.3389\/fphys.2012.00193","volume":"3","author":"VL Massey","year":"2012","unstructured":"Massey, V. L. & Arteel, G. E. Acute alcohol-induced liver injury. Front. Physiol. 3, 193 (2012).","journal-title":"Front. Physiol."},{"key":"7111_CR4","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1038\/s41575-018-0099-1","volume":"16","author":"JS Bajaj","year":"2019","unstructured":"Bajaj, J. S. Alcohol, liver disease and the gut microbiota. Nat. Reviews Gastroenterol. Hepatol. 16, 235\u2013246 (2019).","journal-title":"Nat. Reviews Gastroenterol. Hepatol."},{"key":"7111_CR5","doi-asserted-by":"publisher","first-page":"14918","DOI":"10.3390\/ijms241914918","volume":"24","author":"A Mijangos-Trejo","year":"2023","unstructured":"Mijangos-Trejo, A. et al. Prebiotics and probiotics: therapeutic tools for nonalcoholic fatty liver disease. Int. J. Mol. Sci. 24, 14918 (2023).","journal-title":"Int. J. Mol. Sci."},{"key":"7111_CR6","doi-asserted-by":"publisher","first-page":"1916278","DOI":"10.1080\/19490976.2021.1916278","volume":"13","author":"B Caslin","year":"2021","unstructured":"Caslin, B., Mohler, K., Thiagarajan, S. & Melamed, E. Alcohol as friend or foe in autoimmune diseases: a role for gut microbiome? Gut Microbes. 13, 1916278 (2021).","journal-title":"Gut Microbes"},{"key":"7111_CR7","doi-asserted-by":"publisher","first-page":"1831","DOI":"10.7150\/ijbs.81447","volume":"19","author":"R Li","year":"2023","unstructured":"Li, R. et al. Interaction between dual specificity phosphatase-1 and cullin-1 attenuates alcohol-related liver disease by restoring p62-mediated mitophagy. Int. J. Biol. Sci. 19, 1831 (2023).","journal-title":"Int. J. Biol. Sci."},{"key":"7111_CR8","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1006\/phrs.2001.0883","volume":"44","author":"MC Guti\u00e9rrez-Ruiz","year":"2001","unstructured":"Guti\u00e9rrez-Ruiz, M. C. et al. Metadoxine prevents damage produced by ethanol and acetaldehyde in hepatocyte and hepatic stellate cells in culture. Pharmacol. Res. 44, 431\u2013436. https:\/\/doi.org\/10.1006\/phrs.2001.0883 (2001).","journal-title":"Pharmacol. Res."},{"key":"7111_CR9","doi-asserted-by":"publisher","first-page":"449","DOI":"10.2146\/ajhp090168","volume":"67","author":"NT Williams","year":"2010","unstructured":"Williams, N. T. & Probiotics Am. J. Health-System Pharm. 67, 449\u2013458 (2010).","journal-title":"Am. J. Health-System Pharm."},{"key":"7111_CR10","doi-asserted-by":"publisher","first-page":"2555","DOI":"10.1038\/s41467-022-29968-0","volume":"13","author":"R Cheng","year":"2022","unstructured":"Cheng, R. et al. A randomized controlled trial for response of Microbiome network to exercise and diet intervention in patients with nonalcoholic fatty liver disease. Nat. Commun. 13, 2555 (2022).","journal-title":"Nat. Commun."},{"key":"7111_CR11","doi-asserted-by":"publisher","first-page":"5941","DOI":"10.3748\/wjg.v12.i37.5941","volume":"12","author":"JB Ewaschuk","year":"2006","unstructured":"Ewaschuk, J. B. & Dieleman, L. A. Probiotics and prebiotics in chronic inflammatory bowel diseases. World J. Gastroenterology: WJG. 12, 5941 (2006).","journal-title":"World J. Gastroenterology: WJG"},{"key":"7111_CR12","doi-asserted-by":"publisher","first-page":"G966","DOI":"10.1152\/ajpgi.00380.2011","volume":"302","author":"EA Mutlu","year":"2012","unstructured":"Mutlu, E. A. et al. Colonic Microbiome is altered in alcoholism. Am. J. Physiol. Gastrointest. Liver Physiol. 302, G966\u2013978. https:\/\/doi.org\/10.1152\/ajpgi.00380.2011 (2012).","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"7111_CR13","doi-asserted-by":"publisher","first-page":"G675","DOI":"10.1152\/ajpgi.00152.2012","volume":"303","author":"JS Bajaj","year":"2012","unstructured":"Bajaj, J. S. et al. Colonic mucosal Microbiome differs from stool Microbiome in cirrhosis and hepatic encephalopathy and is linked to cognition and inflammation. Am. J. Physiol. Gastrointest. Liver Physiol. 303, G675\u2013685. https:\/\/doi.org\/10.1152\/ajpgi.00152.2012 (2012).","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"7111_CR14","doi-asserted-by":"publisher","first-page":"48s","DOI":"10.1111\/j.1530-0277.2000.tb00012.x","volume":"24","author":"M Fujimoto","year":"2000","unstructured":"Fujimoto, M. et al. Plasma endotoxin and serum cytokine levels in patients with alcoholic hepatitis: relation to severity of liver disturbance. Alcohol Clin. Exp. Res. 24, 48s\u201354s (2000).","journal-title":"Alcohol Clin. Exp. Res."},{"key":"7111_CR15","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1053\/jhep.2000.19621","volume":"32","author":"P Mathurin","year":"2000","unstructured":"Mathurin, P. et al. Exacerbation of alcoholic liver injury by enteral endotoxin in rats. Hepatology 32, 1008\u20131017 (2000).","journal-title":"Hepatology"},{"key":"7111_CR16","doi-asserted-by":"publisher","first-page":"2866","DOI":"10.1016\/j.ajpath.2011.08.039","volume":"179","author":"Y Wang","year":"2011","unstructured":"Wang, Y. et al. Lactobacillus rhamnosus GG treatment potentiates intestinal hypoxia-inducible factor, promotes intestinal integrity and ameliorates alcohol-induced liver injury. Am. J. Pathol. 179, 2866\u20132875 (2011).","journal-title":"Am. J. Pathol."},{"key":"7111_CR17","doi-asserted-by":"publisher","first-page":"G32","DOI":"10.1152\/ajpgi.00024.2012","volume":"303","author":"Y Wang","year":"2012","unstructured":"Wang, Y. et al. Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury. Am. J. Physiology-Gastrointestinal Liver Physiol. 303, G32\u2013G41 (2012).","journal-title":"Am. J. Physiology-Gastrointestinal Liver Physiol."},{"key":"7111_CR18","doi-asserted-by":"publisher","unstructured":"Dong, Y., Wu, Z., Gai, Z. & Han, M. Bifidobacterium longum subsp. longum BL21 ameliorates alcoholic liver disease in mice through enhancement of the hepatic antioxidant capacity and modulation of the gut microbiota. J. Appl. Microbiol. 134 https:\/\/doi.org\/10.1093\/jambio\/lxad251 (2023).","DOI":"10.1093\/jambio\/lxad251"},{"key":"7111_CR19","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.26599\/FSHW.2022.9250172","volume":"13","author":"H Li","year":"2024","unstructured":"Li, H. et al. Lactobacillus plantarum J26 alleviates alcohol-induced oxidative liver injury by regulating the Nrf2 signaling pathway. Food Sci. Hum. Wellness. 13, 2068\u20132078. https:\/\/doi.org\/10.26599\/FSHW.2022.9250172 (2024).","journal-title":"Food Sci. Hum. Wellness"},{"key":"7111_CR20","unstructured":"Romario-Silva, D. et al. IntechOpen,. in Prebiotics and Probiotics-From Food to Health (2021)."},{"key":"7111_CR21","doi-asserted-by":"publisher","first-page":"561","DOI":"10.3390\/encyclopedia3020040","volume":"3","author":"HN Rabetafika","year":"2023","unstructured":"Rabetafika, H. N., Razafindralambo, A., Ebenso, B. & Razafindralambo, H. L. Probiotics as antibiotic alternatives for human and animal applications. Encyclopedia 3, 561\u2013581 (2023).","journal-title":"Encyclopedia"},{"key":"7111_CR22","doi-asserted-by":"publisher","first-page":"418","DOI":"10.4254\/wjh.v9.i8.418","volume":"9","author":"AC Schneider","year":"2017","unstructured":"Schneider, A. C. et al. Chronic exposure to ethanol causes steatosis and inflammation in zebrafish liver. World J. Hepatol. 9, 418\u2013426. https:\/\/doi.org\/10.4254\/wjh.v9.i8.418 (2017).","journal-title":"World J. Hepatol."},{"key":"7111_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12885-020-07689-1","volume":"20","author":"X Liu","year":"2020","unstructured":"Liu, X. et al. Prognostic implications of alcohol dehydrogenases in hepatocellular carcinoma. BMC cancer. 20, 1\u201313 (2020).","journal-title":"BMC cancer"},{"key":"7111_CR24","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1124\/jpet.118.249425","volume":"367","author":"N Gao","year":"2018","unstructured":"Gao, N. et al. Higher activity of alcohol dehydrogenase is correlated with hepatic fibrogenesis. J. Pharmacol. Exp. Ther. 367, 473\u2013482 (2018).","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"7111_CR25","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1111\/j.1365-2362.2011.02573.x","volume":"42","author":"G Poelzl","year":"2012","unstructured":"Poelzl, G. et al. Liver dysfunction in chronic heart failure: prevalence, characteristics and prognostic significance. Eur. J. Clin. Invest. 42, 153\u2013163 (2012).","journal-title":"Eur. J. Clin. Invest."},{"key":"7111_CR26","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S1386-6346(99)00085-6","volume":"18","author":"FH Anderson","year":"2000","unstructured":"Anderson, F. H., Zeng, L., Rock, N. R. & Yoshida, E. M. An assessment of the clinical utility of serum ALT and AST in chronic hepatitis C. Hepatol. Res. 18, 63\u201371 (2000).","journal-title":"Hepatol. Res."},{"key":"7111_CR27","doi-asserted-by":"crossref","unstructured":"Andy, S. Y. & Keeffe, E. B. Vol. 98 955\u2013956 (LWW, (2003).","DOI":"10.1111\/j.1572-0241.2003.07485.x"},{"key":"7111_CR28","unstructured":"Seebacher, F., Zeigerer, A., Kory, N. & Krahmer, N. in Seminars Cell. & Dev. Biology 72\u201381 (Elsevier)."},{"key":"7111_CR29","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/0014-4827(72)90400-4","volume":"74","author":"P Seglen","year":"1972","unstructured":"Seglen, P. Preparation of rat liver cells: I. Effect of Ca2\u2009+\u2009on enzymatic dispersion of isolated, perfused liver. Exp. Cell Res. 74, 450\u2013454 (1972).","journal-title":"Exp. Cell Res."},{"key":"7111_CR30","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1093\/geronj\/31.2.129","volume":"31","author":"EL Tate","year":"1976","unstructured":"Tate, E. L. & Herbener, G. H. A morphometric study of the density of mitochondrial Cristae in heart and liver of aging mice. J. Gerontol. 31, 129\u2013134 (1976).","journal-title":"J. Gerontol."},{"key":"7111_CR31","doi-asserted-by":"publisher","first-page":"67","DOI":"10.4103\/abr.abr_142_17","volume":"7","author":"P Mazraati","year":"2018","unstructured":"Mazraati, P. & Minaiyan, M. Hepatoprotective effect of metadoxine on acetaminophen-induced liver toxicity in mice. Adv. Biomedical Res. 7, 67 (2018).","journal-title":"Adv. Biomedical Res."},{"key":"7111_CR32","doi-asserted-by":"publisher","first-page":"493","DOI":"10.3390\/biom10030493","volume":"10","author":"T Mart\u00ednez-Sena","year":"2020","unstructured":"Mart\u00ednez-Sena, T. et al. The vitamin D receptor regulates glycerolipid and phospholipid metabolism in human hepatocytes. Biomolecules 10, 493 (2020).","journal-title":"Biomolecules"},{"key":"7111_CR33","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1016\/j.fsi.2022.07.063","volume":"130","author":"M Pan","year":"2022","unstructured":"Pan, M. et al. Biotin alleviates hepatic and intestinal inflammation and apoptosis induced by high dietary carbohydrate in juvenile turbot (Scophthalmus Maximus L). Fish Shellfish Immunol. 130, 560\u2013571 (2022).","journal-title":"Fish Shellfish Immunol."},{"key":"7111_CR34","doi-asserted-by":"publisher","first-page":"250","DOI":"10.3177\/jnsv.68.250","volume":"68","author":"M Yuasa","year":"2022","unstructured":"Yuasa, M. et al. Dietary High-Dose biotin intake activates fat oxidation and hepatic carnitine palmitoyltransferase in rat. J. Nutri. Sci. Vitaminol. 68, 250\u2013259 (2022).","journal-title":"J. Nutri. Sci. Vitaminol."},{"key":"7111_CR35","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.2174\/0929867325666180327092639","volume":"26","author":"S Villanueva","year":"2019","unstructured":"Villanueva, S., Zhang, W., Zecchinati, F., Mottino, A. & Vore, M. ABC transporters in extrahepatic tissues: Pharmacological regulation in heart and intestine. Curr. Med. Chem. 26, 1155\u20131184 (2019).","journal-title":"Curr. Med. Chem."},{"key":"7111_CR36","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.tice.2018.02.001","volume":"51","author":"SK Vishwakarma","year":"2018","unstructured":"Vishwakarma, S. K. et al. Role of drug transporters and heat shock proteins during ethanol exposure to human neural precursor cells and its lineages. Tissue Cell. 51, 14\u201323 (2018).","journal-title":"Tissue Cell."},{"key":"7111_CR37","doi-asserted-by":"publisher","first-page":"1032127","DOI":"10.3389\/fmed.2022.1032127","volume":"9","author":"B Lian","year":"2022","unstructured":"Lian, B. et al. Qin-Qiao-Xiao-Du formula alleviate influenza virus infectious pneumonia through regulation gut microbiota and metabolomics. Front. Med. 9, 1032127 (2022).","journal-title":"Front. Med."},{"key":"7111_CR38","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1186\/s13578-023-00974-z","volume":"13","author":"L Chen","year":"2023","unstructured":"Chen, L. et al. Modulating phenylalanine metabolism by L. acidophilus alleviates alcohol-related liver disease through enhancing intestinal barrier function. Cell. Bioscience. 13, 24 (2023).","journal-title":"Cell. Bioscience"},{"key":"7111_CR39","doi-asserted-by":"publisher","first-page":"132372","DOI":"10.1016\/j.foodchem.2022.132372","volume":"384","author":"A Asmaa\u2019Ishak","year":"2022","unstructured":"Asmaa\u2019Ishak, A. et al. Simultaneous kinetics formation of heterocyclic amines and polycyclic aromatic hydrocarbons in phenylalanine model system. Food Chem. 384, 132372 (2022).","journal-title":"Food Chem."},{"key":"7111_CR40","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1264\/jsme2.me10179","volume":"26","author":"HJ Doughari","year":"2011","unstructured":"Doughari, H. J., Ndakidemi, P. A., Human, I. S. & Benade, S. The ecology, biology and pathogenesis of Acinetobacter spp.: an overview. Microbes Environ. 26, 101\u2013112. https:\/\/doi.org\/10.1264\/jsme2.me10179 (2011).","journal-title":"Microbes Environ."},{"key":"7111_CR41","doi-asserted-by":"publisher","unstructured":"Quesada-V\u00e1zquez, S. et al. Microbiota Dysbiosis and Gut Barrier Dysfunction Associated with Non-Alcoholic Fatty Liver Disease Are Modulated by a Specific Metabolic Cofactors\u2019 Combination. Int. J. Mol. Sci. 23 https:\/\/doi.org\/10.3390\/ijms232213675 (2022).","DOI":"10.3390\/ijms232213675"},{"key":"7111_CR42","doi-asserted-by":"publisher","first-page":"1493560","DOI":"10.3389\/fimmu.2025.1493560","volume":"16","author":"Y Dong","year":"2025","unstructured":"Dong, Y. et al. Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage. Front. Immunol. 16, 1493560 (2025).","journal-title":"Front. Immunol."},{"key":"7111_CR43","first-page":"81","volume":"47","author":"NK Kharchenko","year":"2001","unstructured":"Kharchenko, N. K., Synyts\u2019ky\u012d, V. N. & Kovtun, T. V. [Comparative analysis of the effects of alcoholism and opium addiction on liver function]. Fiziol. Zh (1994). 47, 81\u201386 (2001).","journal-title":"Fiziol. Zh (1994)"},{"key":"7111_CR44","unstructured":"WenCong, D., Li, L. & Kunyuan, W. Establishment of a zebrafish model of alcohol-induced acute hepatic steatosis. Journal Clin. Hepatology\/Linchuang Gandanbing Zazhi 29 (2013)."},{"key":"7111_CR45","doi-asserted-by":"publisher","first-page":"1139","DOI":"10.1002\/jat.4607","volume":"44","author":"X Ni","year":"2024","unstructured":"Ni, X. et al. Isobavachalcone induces hepatotoxicity in zebrafish embryos and HepG2 cells via the system Xc(-)-GSH-GPX4 signaling pathway in ferroptosis response. J. Appl. Toxicol. 44, 1139\u20131152. https:\/\/doi.org\/10.1002\/jat.4607 (2024).","journal-title":"J. Appl. Toxicol."},{"key":"7111_CR46","doi-asserted-by":"publisher","first-page":"12355","DOI":"10.1021\/acs.analchem.5b03812","volume":"87","author":"H Gu","year":"2015","unstructured":"Gu, H., Zhang, P., Zhu, J. & Raftery, D. Globally optimized targeted mass spectrometry: reliable metabolomics analysis with broad coverage. Anal. Chem. 87, 12355\u201312362 (2015).","journal-title":"Anal. Chem."},{"key":"7111_CR47","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1038\/ncb3264","volume":"17","author":"H Sperber","year":"2015","unstructured":"Sperber, H. et al. The metabolome regulates the epigenetic landscape during naive-to-primed human embryonic stem cell transition. Nat. Cell Biol. 17, 1523\u20131535 (2015).","journal-title":"Nat. Cell Biol."},{"key":"7111_CR48","doi-asserted-by":"publisher","first-page":"163770","DOI":"10.1016\/j.scitotenv.2023.163770","volume":"887","author":"M Xu","year":"2023","unstructured":"Xu, M., Legradi, J. & Leonards, P. A comprehensive untargeted metabolomics study in zebrafish embryos exposed to perfluorohexane sulfonate (PFHxS). Sci. Total Environ. 887, 163770. https:\/\/doi.org\/10.1016\/j.scitotenv.2023.163770 (2023).","journal-title":"Sci. Total Environ."},{"key":"7111_CR49","doi-asserted-by":"publisher","first-page":"115310","DOI":"10.1016\/j.envpol.2020.115310","volume":"266","author":"J Zhang","year":"2020","unstructured":"Zhang, J. et al. UPLC-TOF-MS\/MS metabolomics analysis of zebrafish metabolism by spirotetramat. Environ. Pollut. 266, 115310. https:\/\/doi.org\/10.1016\/j.envpol.2020.115310 (2020).","journal-title":"Environ. Pollut"},{"key":"7111_CR50","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1002\/wics.147","volume":"3","author":"H Wickham","year":"2011","unstructured":"Wickham, H. ggplot2. Wiley Interdisciplinary Reviews: Comput. Stat. 3, 180\u2013185 (2011).","journal-title":"Wiley Interdisciplinary Reviews: Comput. Stat."},{"key":"7111_CR51","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1016\/j.dib.2016.06.057","volume":"8","author":"DJ Davis","year":"2016","unstructured":"Davis, D. J., Bryda, E. C., Gillespie, C. H. & Ericsson, A. C. 16S rRNA amplicon sequencing dataset for conventionalized and conventionally Raised zebrafish larvae. Data Brief. 8, 938\u2013943 (2016).","journal-title":"Data Brief."},{"key":"7111_CR52","doi-asserted-by":"publisher","first-page":"e83","DOI":"10.1002\/imt2.83","volume":"2","author":"YX Liu","year":"2023","unstructured":"Liu, Y. X. et al. EasyAmplicon: an easy-to\u2010use, open\u2010source, reproducible, and community\u2010based pipeline for amplicon data analysis in Microbiome research. IMeta 2, e83 (2023).","journal-title":"IMeta"},{"key":"7111_CR53","unstructured":"Edgar, R. Usearch. (Lawrence Berkeley National Lab.(LBNL), Berkeley, CA (United States), (2010)."},{"key":"7111_CR54","doi-asserted-by":"publisher","first-page":"D672","DOI":"10.1093\/nar\/gkae909","volume":"53","author":"M Kanehisa","year":"2024","unstructured":"Kanehisa, M., Furumichi, M., Sato, Y., Matsuura, Y. & Ishiguro-Watanabe, M. KEGG: biological systems database as a model of the real world. Nucleic Acids Res. 53, D672\u2013D677. https:\/\/doi.org\/10.1093\/nar\/gkae909 (2024).","journal-title":"Nucleic Acids Res."},{"key":"7111_CR55","doi-asserted-by":"publisher","first-page":"1947","DOI":"10.1002\/pro.3715","volume":"28","author":"M Kanehisa","year":"2019","unstructured":"Kanehisa, M. Toward Understanding the origin and evolution of cellular organisms. Protein Sci. 28, 1947\u20131951. https:\/\/doi.org\/10.1002\/pro.3715 (2019).","journal-title":"Protein Sci."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07111-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07111-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07111-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T12:02:17Z","timestamp":1751803337000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-07111-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":55,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["7111"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-07111-5","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,2]]},"assertion":[{"value":"20 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This experiment received an animal use permit (Permit Number: SYXK (Zhe) 2022-0004) and strictly adhered to the standards of the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) (Certification Number: 001458). All experimental procedures were ethically reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) (Review Number: IACUC-2024-8511-01). The study was conducted in accordance with ARRIVE guidelines (). No human studies are part of this research, hence consent to participate is not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}}],"article-number":"22584"}}