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In this study, four endophytic fungi were isolated from <jats:italic>Olea europaea<\/jats:italic> and their ethyl acetate extracts\u2019 larvicidal efficacy of <jats:italic>Culex pipiens<\/jats:italic>. Boxplot distributions revealed a positive correlation between metabolite concentration and larval mortality, with higher doses inducing greater lethality. Extracts 1 and 2 exhibited the highest larvicidal activity, with median mortality rates exceeding 60%, while Extracts 3 and 4 demonstrated significantly weaker effects. Dose-response curves further confirmed the potency of Extracts 1 and 2, with lower LC\u2085\u2080 values (148.36 and 153.36\u00a0\u00b5g\/mL) compared to Extracts 3 and 4. The histological analysis revealed a dose-dependent impact, with minimal tissue alterations at 25\u201350\u00a0\u00b5g\/mL, while 150\u2013200\u00a0\u00b5g\/mL caused severe disorganization, apoptosis, and inflammatory responses. The most potent isolates, <jats:italic>Aspergillus niger<\/jats:italic>(Extract 1) and <jats:italic>Aspergillus flavus<\/jats:italic>(Extract 2), were identified morphologically and molecularly, with NCBI accession numbers PQ269689 and PQ269690, respectively. GC-MS analysis identified key bioactive compounds, including oleic acid and hexadecanoic acid, supporting their insecticidal potential. These findings highlight <jats:italic>A. niger<\/jats:italic> and <jats:italic>A. flavus<\/jats:italic> ethyl acetate extracts as promising sources of bioinsecticides for pest control.<\/jats:p>","DOI":"10.1038\/s41598-025-07941-3","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T17:10:24Z","timestamp":1751649024000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Bioinsecticidal activity of aspergillus-derived endophytes from Olea europaea against Culex pipiens: toxicity, histology, and GC-MS profiling"],"prefix":"10.1038","volume":"15","author":[{"given":"Amr H.","family":"Hashem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesam M. 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