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This study aimed to comprehensively assess the prevalence of cryptosporidiosis, giardiasis, and co-infections in Beni-Suef Governorate, Egypt, using an integrated diagnostic approach combining microscopy and molecular techniques. Additionally, it was sought to identify associated risk factors in cattle fecal samples. Microscopical examination of 970 cattle fecal samples revealed an overall infection rate of 67.42% (654\/970), with\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    detected in 42.68% (414\/970),\n                    <jats:italic>Giardia<\/jats:italic>\n                    in 11.96% (116\/970), and co-infections in 12.78% (124\/970) of cases. In irrigation water,\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    oocysts and\n                    <jats:italic>Giardia<\/jats:italic>\n                    cysts were detected in 2\/24 (8.33%) and 1\/24 (4.16%) of samples, respectively. Molecular and phylogenetic analyses identified\n                    <jats:italic>Cryptosporidium hominis<\/jats:italic>\n                    in cattle and, for the first time in Egypt,\n                    <jats:italic>Cryptosporidium ubiquitum<\/jats:italic>\n                    and\n                    <jats:italic>Cryptosporidium ryanae<\/jats:italic>\n                    in irrigation water, while also proving the presence of\n                    <jats:italic>Cryptosporidium bovis<\/jats:italic>\n                    and\n                    <jats:italic>Giardia<\/jats:italic>\n                    assemblage A in cattle. Risk factors, including sex, age, season, and fecal consistency, significantly influenced infection rates, with higher prevalence in females, calves under two months, spring season, and diarrheic feces. These findings underscore the urgent need for One Health-based control strategies, integrating targeted interventions to mitigate the burden of\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    and\n                    <jats:italic>Giardia<\/jats:italic>\n                    infections and environmental contamination.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-10552-7","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T16:10:20Z","timestamp":1753373420000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Coprological and molecular prevalence of Cryptosporidium and Giardia in cattle and irrigation water from Beni-Suef Governorate, Egypt"],"prefix":"10.1038","volume":"15","author":[{"given":"Fatma El-zahraa Ramadan","family":"Saleh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hend H. 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