{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:15:26Z","timestamp":1793142926475,"version":"4.5.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-10636-4","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T13:22:17Z","timestamp":1755782537000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Validation and standardization of the content of three di-caffeoylquinic acid in Korean Ligularia fischeri by region of origin"],"prefix":"10.1038","volume":"15","author":[{"given":"Hun Hwan","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Hyo","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pritam Bhangwan","family":"Bhosale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Yang","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Woo","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Il","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon Gyu","family":"Moon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Hyun","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Il","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meejung","family":"Ahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je Kyung","family":"Seong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gon Sup","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"10636_CR1","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1186\/0717-6287-47-69","volume":"47","author":"HS Park","year":"2014","unstructured":"Park, H. S., Choi, H. Y. & Kim, G. H. Preventive effect of Ligularia Fischerion Inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages depending on cooking method. Biol. Res. 47, 69. https:\/\/doi.org\/10.1186\/0717-6287-47-69 (2014).","journal-title":"Biol. Res."},{"key":"10636_CR2","doi-asserted-by":"publisher","unstructured":"Kim, T. H., Truong, V. L. & Jeong, W. S. Phytochemical composition and antioxidant and Anti-Inflammatory activities of Ligularia fischeri turcz: A comparison between leaf and root extracts. Plants (Basel Switzerland). 11 https:\/\/doi.org\/10.3390\/plants11213005 (2022).","DOI":"10.3390\/plants11213005"},{"key":"10636_CR3","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1007\/s10600-016-1729-x","volume":"52","author":"X Liu","year":"2016","unstructured":"Liu, X. et al. A new sesquiterpene from Ligularia fischeri. Chem. Nat. Compd. 52, 642\u2013646. https:\/\/doi.org\/10.1007\/s10600-016-1729-x (2016).","journal-title":"Chem. Nat. Compd."},{"key":"10636_CR4","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1140509","author":"YJ Park","year":"2023","unstructured":"Park, Y. J. et al. Identification of drought-responsive phenolic compounds and their biosynthetic regulation under drought stress in Ligularia fischeri. 14\u20132023 https:\/\/doi.org\/10.3389\/fpls.2023.1140509 (2023).","journal-title":"14\u20132023"},{"key":"10636_CR5","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1007\/s10600-019-02767-8","volume":"55","author":"X Liu","year":"2019","unstructured":"Liu, X., Li, J., Li, J., Liu, Q. & Xun, M. A new flavonoid glycoside from Ligularia fischeri. Chem. Nat. Compd. 55, 638\u2013641. https:\/\/doi.org\/10.1007\/s10600-019-02767-8 (2019).","journal-title":"Chem. Nat. Compd."},{"key":"10636_CR6","doi-asserted-by":"publisher","unstructured":"Shanmugam, G. Polyphenols: potent protectors against chronic diseases. Nat. Prod. Res. 1\u20133. https:\/\/doi.org\/10.1080\/14786419.2024.2386402 (2024).","DOI":"10.1080\/14786419.2024.2386402"},{"key":"10636_CR7","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1007\/s13258-013-0124-2","volume":"35","author":"YR Cho","year":"2013","unstructured":"Cho, Y. R., Kim, J. K., Kim, J. H., Oh, J. S. & Seo, D. W. Ligularia fischeri regulates lung cancer cell proliferation and migration through down-regulation of epidermal growth factor receptor and integrin \u03b21 expression. Genes Genomics. 35, 741\u2013746. https:\/\/doi.org\/10.1007\/s13258-013-0124-2 (2013).","journal-title":"Genes Genomics"},{"key":"10636_CR8","doi-asserted-by":"publisher","unstructured":"Baek, H. J. et al. Antihyperglycemic and Antilipidemic Effects of the Ethanol Extract Mixture of Ligularia fischeri and Momordica charantia in Type II Diabetes-Mimicking Mice. Evidence-based complementary and alternative medicine: eCAM 2018, 3468040, (2018). https:\/\/doi.org\/10.1155\/2018\/3468040","DOI":"10.1155\/2018\/3468040"},{"key":"10636_CR9","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1007\/s10068-011-0228-x","volume":"20","author":"JH Yoo","year":"2011","unstructured":"Yoo, J. H. et al. Hepatoprotective effect of Handaeri-gomchi (Ligularia fischeri var. Spiciformis Nakai) extract against chronic alcohol-induced liver damage in rats. Food Sci. Biotechnol. 20, 1655\u20131661. https:\/\/doi.org\/10.1007\/s10068-011-0228-x (2011).","journal-title":"Food Sci. Biotechnol."},{"key":"10636_CR10","doi-asserted-by":"publisher","first-page":"1575","DOI":"10.1002\/ptr.2823","volume":"23","author":"EM Choi","year":"2009","unstructured":"Choi, E. M. & Suh, K. S. Ligularia fischeri leaf extract suppresses Proinflammatory mediators in SW982 human synovial cells. Phytother Res. 23, 1575\u20131580. https:\/\/doi.org\/10.1002\/ptr.2823 (2009).","journal-title":"Phytother Res."},{"key":"10636_CR11","doi-asserted-by":"publisher","first-page":"4968","DOI":"10.3390\/nu15234968","volume":"15","author":"AR Jang","year":"2023","unstructured":"Jang, A. R. et al. Water extract of desalted Salicornia europaea inhibits RANKL-Induced osteoclast differentiation and prevents bone loss in ovariectomized mice. Nutrients 15, 4968. https:\/\/doi.org\/10.3390\/nu15234968 (2023).","journal-title":"Nutrients"},{"key":"10636_CR12","doi-asserted-by":"publisher","unstructured":"Shang, Y. F. et al. Isolation and identification of antioxidant compounds from\u2002ligularia fischeri. 75, C530\u2013C535, (2010). https:\/\/doi.org\/10.1111\/j.1750-3841.2010.01714.x","DOI":"10.1111\/j.1750-3841.2010.01714.x"},{"key":"10636_CR13","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1556\/018.66.2015.2.5","volume":"66","author":"K Rekha","year":"2015","unstructured":"Rekha, K., Sivasubramanian, C. & Thiruvengadam, M. Evaluation of polyphenol composition and biological activities of two samples from summer and winter seasons of Ligularia fischeri var. Spiciformis Nakai %J Acta Biologica Hungarica Acta Biologica Hungarica. 66, 179\u2013191. https:\/\/doi.org\/10.1556\/018.66.2015.2.5 (2015).","journal-title":"Spiciformis Nakai %J Acta Biologica Hungarica Acta Biologica Hungarica"},{"key":"10636_CR14","doi-asserted-by":"publisher","first-page":"4065","DOI":"10.3390\/molecules24224065","volume":"24","author":"L Yang","year":"2019","unstructured":"Yang, L., Li, C. L., Cheng, Y. Y. & Tsai, T. H. Development of a validated UPLC-MS\/MS method for analyzing major ginseng saponins from various ginseng species. Molecules. 24, 4065 (2019).","journal-title":"Molecules"},{"key":"10636_CR15","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1093\/jaoacint\/qsac042","volume":"105","author":"A Tiwari","year":"2022","unstructured":"Tiwari, A., Bose, D., Mishra, P., Jain, A. & Jain, S. K. Determination of oxaliplatin and Curcumin in combination via micellar HPLC and its method validation. J. AOAC Int. 105, 999\u20131007. https:\/\/doi.org\/10.1093\/jaoacint\/qsac042 (2022).","journal-title":"J. AOAC Int."},{"key":"10636_CR16","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.apsb.2015.11.001","volume":"6","author":"G Liu","year":"2016","unstructured":"Liu, G. et al. Development and validation of an HPLC-MS\/MS method to determine clopidogrel in human plasma. Acta Pharm. Sinica B. 6, 55\u201363. https:\/\/doi.org\/10.1016\/j.apsb.2015.11.001 (2016).","journal-title":"Acta Pharm. Sinica B"},{"key":"10636_CR17","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.aca.2016.05.041","volume":"933","author":"JL Willems","year":"2016","unstructured":"Willems, J. L. et al. Analysis of a series of chlorogenic acid isomers using differential ion mobility and tandem mass spectrometry. Anal. Chim. Acta. 933, 164\u2013174. https:\/\/doi.org\/10.1016\/j.aca.2016.05.041 (2016).","journal-title":"Anal. Chim. Acta"},{"key":"10636_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5455\/jice.20171011073247","volume":"7","author":"H Salman","year":"2018","unstructured":"Salman, H., Ramasamy, S. & Mahmood, B. Detection of caffeic and chlorogenic acids from methanolic extract of Annona squamosa bark by LC-ESI-MS\/MS. J. Intercultural Ethnopharmacol. 7, 1. https:\/\/doi.org\/10.5455\/jice.20171011073247 (2018).","journal-title":"J. Intercultural Ethnopharmacol."},{"key":"10636_CR19","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1590\/0001-37652013105312","volume":"85","author":"JL Santos","year":"2013","unstructured":"Santos, J. L. et al. Evaluation of chemical constituents and antioxidant activity of coconut water (Cocus nucifera L.) and caffeic acid in cell culture. Anais Da Acad. Brasileira De Ciencias. 85, 1235\u20131247. https:\/\/doi.org\/10.1590\/0001-37652013105312 (2013).","journal-title":"Anais Da Acad. Brasileira De Ciencias"},{"key":"10636_CR20","doi-asserted-by":"publisher","first-page":"934","DOI":"10.1016\/j.jpba.2011.03.024","volume":"55","author":"F Pellati","year":"2011","unstructured":"Pellati, F., Orlandini, G., Pinetti, D., Benvenuti, S. & HPLC-DAD HPLC-ESI-MS\/MS methods for metabolite profiling of propolis extracts. J. Pharm. Biomed. Anal. 55, 934\u2013948. https:\/\/doi.org\/10.1016\/j.jpba.2011.03.024 (2011).","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10636_CR21","doi-asserted-by":"publisher","first-page":"3299","DOI":"10.3390\/molecules27103299","volume":"27","author":"S Cao","year":"2022","unstructured":"Cao, S. et al. Chemical constituent analysis of Ranunculus sceleratus L. Using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry. Molecules 27, 3299 (2022).","journal-title":"Molecules"},{"key":"10636_CR22","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1186\/s12934-024-02390-5","volume":"23","author":"T Niu","year":"2024","unstructured":"Niu, T. et al. Combinatorial metabolic engineering of Bacillus subtilis enables the efficient biosynthesis of isoquercitrin from Quercetin. Microb. Cell. Fact. 23, 114. https:\/\/doi.org\/10.1186\/s12934-024-02390-5 (2024).","journal-title":"Microb. Cell. Fact."},{"key":"10636_CR23","doi-asserted-by":"publisher","first-page":"6431","DOI":"10.1039\/C3AY40681D","volume":"5","author":"L Sun","year":"2013","unstructured":"Sun, L. et al. Qualitative analysis and quality control of traditional Chinese medicine Preparation Tanreqing injection by LC-TOF\/MS and HPLC-DAD-ELSD. Anal. Methods. 5, 6431\u20136440. https:\/\/doi.org\/10.1039\/C3AY40681D (2013).","journal-title":"Anal. Methods"},{"key":"10636_CR24","doi-asserted-by":"publisher","first-page":"3821","DOI":"10.1021\/jf050046h","volume":"53","author":"MN Clifford","year":"2005","unstructured":"Clifford, M. N., Knight, S. & Kuhnert, N. Discriminating between the six isomers of Dicaffeoylquinic acid by LC-MSn. J. Agric. Food Chem. 53, 3821\u20133832. https:\/\/doi.org\/10.1021\/jf050046h (2005).","journal-title":"J. Agric. Food Chem."},{"key":"10636_CR25","doi-asserted-by":"publisher","first-page":"3859","DOI":"10.3390\/molecules18043859","volume":"18","author":"RG Peres","year":"2013","unstructured":"Peres, R. G., Tonin, F. G., Tavares, M. F. & Rodriguez-Amaya, D. B. HPLC-DAD-ESI\/MS identification and quantification of phenolic compounds in Ilex paraguariensis beverages and on-line evaluation of individual antioxidant activity. Molecules 18, 3859\u20133871. https:\/\/doi.org\/10.3390\/molecules18043859 (2013).","journal-title":"Molecules"},{"key":"10636_CR26","doi-asserted-by":"publisher","unstructured":"Misto, M. et al. Identification of chlorogenic acid, caffeine, melanoidin, sucrose, and protein content of local Indonesia Arabica coffee base on its cupping and variety variation. BIO Web Conferences. 101 (01003). https:\/\/doi.org\/10.1051\/bioconf\/202410101003 (2024).","DOI":"10.1051\/bioconf\/202410101003"},{"key":"10636_CR27","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/s10068-015-0034-y","volume":"24","author":"S Hong","year":"2015","unstructured":"Hong, S., Joo, T. & Jhoo, J. W. Antioxidant and anti-inflammatory activities of 3,5-dicaffeoylquinic acid isolated from Ligularia fischeri leaves. Food Sci. Biotechnol. 24, 257\u2013263. https:\/\/doi.org\/10.1007\/s10068-015-0034-y (2015).","journal-title":"Food Sci. Biotechnol."},{"key":"10636_CR28","doi-asserted-by":"publisher","first-page":"399","DOI":"10.3746\/jkfn.2010.39.3.399","volume":"39","author":"K Sang-Min","year":"2010","unstructured":"Sang-Min, K., Suk-Woo, K. & Byung-Hun, U. Extraction conditions of radical scavenging caffeoylquinic acids from Gomchui (Ligularia fischeri) tea. J. Korean Soc. Food Sci. Nutr. 39, 399\u2013405 (2010).","journal-title":"J. Korean Soc. Food Sci. Nutr."},{"key":"10636_CR29","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.bjp.2018.01.010","volume":"28","author":"IF Mijangos-Ramos","year":"2018","unstructured":"Mijangos-Ramos, I. F. et al. Bioactive Dicaffeoylquinic acid derivatives from the root extract of Calea urticifolia. Revista Brasileira De Farmacognosia. 28, 339\u2013343. https:\/\/doi.org\/10.1016\/j.bjp.2018.01.010 (2018).","journal-title":"Revista Brasileira De Farmacognosia"},{"key":"10636_CR30","doi-asserted-by":"publisher","first-page":"C530","DOI":"10.1111\/j.1750-3841.2010.01714.x","volume":"75","author":"YF Shang","year":"2010","unstructured":"Shang, Y. F. et al. Isolation and identification of antioxidant compounds from Ligularia fischeri. J. Food Sci. 75, C530\u2013535. https:\/\/doi.org\/10.1111\/j.1750-3841.2010.01714.x (2010).","journal-title":"J. Food Sci."},{"key":"10636_CR31","doi-asserted-by":"publisher","first-page":"61","DOI":"10.3746\/jkfn.2016.45.1.061","volume":"45","author":"K Jin Gwan","year":"2016","unstructured":"Jin Gwan, K. et al. Joa sub, O. Method for validation of caffeoylquinic acid derivatives in Ligularia fischeri leaf extract as functional ingredients. J. Korean Soc. Food Sci. Nutr. 45, 61\u201367 (2016).","journal-title":"J. Korean Soc. Food Sci. Nutr."},{"key":"10636_CR32","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.11002\/kjfp.2017.24.8.1113","volume":"24","author":"YJ Woo","year":"2017","unstructured":"Woo, Y. J., Shin, S. R. & Hong, J. Y. Study on antioxidant and physiological activities of extract from Ligularia fischeri by extraction methods. Korean J. Food Preservation. 24, 1113\u20131121. https:\/\/doi.org\/10.11002\/kjfp.2017.24.8.1113 (2017).","journal-title":"Korean J. Food Preservation"},{"key":"10636_CR33","unstructured":"Vidushi, Y., Meenakshi, B., Bharkatiya, M. J. R. J. L. S. & Bioinform Pharm Chem Sci. A review on HPLC method development and validation. 2, 178. (2017)."},{"key":"10636_CR34","unstructured":"Lindholm, J. Development and Validation of HPLC Methods for Analytical and Preparative Purposes (Acta Universitatis Upsaliensis, 2004)."},{"key":"10636_CR35","doi-asserted-by":"publisher","first-page":"1140509","DOI":"10.3389\/fpls.2023.1140509","volume":"14","author":"YJ Park","year":"2023","unstructured":"Park, Y. J. et al. Identification of drought-responsive phenolic compounds and their biosynthetic regulation under drought stress in Ligularia fischeri. Front. Plant. Sci. 14, 1140509. https:\/\/doi.org\/10.3389\/fpls.2023.1140509 (2023).","journal-title":"Front. Plant. Sci."},{"key":"10636_CR36","doi-asserted-by":"publisher","first-page":"89","DOI":"10.3839\/jabc.2022.012","volume":"65","author":"J Seo","year":"2022","unstructured":"Seo, J., Kim, S., Yoo, J., Kim, M. & Seong, E. S. Soil conditions during cultivation affect the total phenolic and flavonoid content of Rosemary. J. Appl. Biol. Chem. 65, 89\u201392. https:\/\/doi.org\/10.3839\/jabc.2022.012 (2022).","journal-title":"J. Appl. Biol. Chem."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-10636-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-10636-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-10636-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T10:09:29Z","timestamp":1757412569000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-10636-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,21]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["10636"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-10636-4","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-6576668\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,21]]},"assertion":[{"value":"2 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 August 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"30760"}}