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Whole-genome sequencing of the most efficient xylose-fermenting strain, A107, revealed mutations in the\n                    <jats:italic>API1<\/jats:italic>\n                    and\n                    <jats:italic>IRA1<\/jats:italic>\n                    genes, which are homologous to bacterial arabinose-5-phosphate isomerase and the Ras-GTPase activating domain in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    , respectively. Disruption of the\n                    <jats:italic>IRA1<\/jats:italic>\n                    gene increased ethanol production during the fermentation of xylose and L-arabinose in\n                    <jats:italic>O. polymorpha<\/jats:italic>\n                    at 45\u00a0\u00b0C. Overexpression of the\n                    <jats:italic>API1<\/jats:italic>\n                    gene specifically enhanced L-arabinose fermentation without affecting xylose fermentation. The most productive mutant strain accumulated 20.91\u00a0g\/L of ethanol in a xylose-containing medium at 45\u00a0\u00b0C, exceeding the ethanol accumulation level of the wild-type strain (0.40\u00a0g\/L) by over 50 times. This strain holds potential for application in simultaneous saccharification and fermentation (SSF) processes.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-12204-2","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:32:50Z","timestamp":1753144370000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Positive selection of efficient ethanol producers from xylose at 45\u00a0\u00b0C in the yeast Ogataea polymorpha"],"prefix":"10.1038","volume":"15","author":[{"given":"Roksolana","family":"Vasylyshyn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Ruchala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kostyantyn","family":"Dmytruk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andriy","family":"Sibirny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"12204_CR1","doi-asserted-by":"publisher","first-page":"105798","DOI":"10.1016\/j.jece.2021.105798","volume":"9","author":"A Devi","year":"2021","unstructured":"Devi, A., Singh, A., Pant, D. & Sheikh, Z. 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