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ROS induce increased oxidative stress and lipid peroxidation, thereby disrupting the structural integrity of myocytes and the functions of skeletal muscles in general. It is hypothesized that biocompatible and bioavailable C<jats:sub>60<\/jats:sub> fullerenes, as potent antioxidants, can effectively absorb ROS, normalizing the functional state of the muscular system during the chronic alcoholic myopathy (CAM). Here, for the first time, the effect of C<jats:sub>60<\/jats:sub> fullerenes (oral daily dose of 1\u00a0mg\/kg) administered together with alcohol (40% ethanol in drinking water) on the contractile activity of the <jats:italic>musculus gastrocnemius<\/jats:italic> in male Wistar rats (age 1 month, weight 170\u2009\u00b1\u200910\u00a0g; <jats:italic>n<\/jats:italic>\u2009=\u200936) during the development of CAM over 3, 6, and 9 months was analyzed using tensometry. Biochemical analysis was used to evaluate pro- and antioxidant balance indicators in the blood of alcohol-exposed animals under the influence of C<jats:sub>60<\/jats:sub> fullerenes. Finally, the potential mechanism of action of C<jats:sub>60<\/jats:sub> fullerenes under chronic alcohol intoxication in rats was analyzed using a computer simulation technique. The data obtained indicate an improvement in the studied biomechanical markers of contraction in alcohol-exposed <jats:italic>musculus gastrocnemius<\/jats:italic> with a range of 16\u201350\u2009\u00b1\u20093%. Additionally, significant biochemical changes were observed in the pro- and antioxidant balance in the blood of experimental rats, showing an improvement of 22\u201339\u2009\u00b1\u20092% due to the action of C<jats:sub>60<\/jats:sub> fullerenes, compared to the alcohol-exposed group. It is demonstrated that C<jats:sub>60<\/jats:sub> fullerene nanoparticles can bind ethanol molecules, thereby reducing the negative effects of alcohol on the functioning of the muscular system.<\/jats:p>","DOI":"10.1038\/s41598-025-16765-0","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T20:06:41Z","timestamp":1758658001000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The effect of C60 fullerene on the musculus gastrocnemius contraction in chronically alcohol-exposed rats and the potential mechanism of its action"],"prefix":"10.1038","volume":"15","author":[{"given":"Olexandr","family":"Motuziuk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmytro","family":"Nozdrenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svitlana","family":"Prylutska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Tverdokhleb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mykola","family":"Petrovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergiy","family":"Vyzhva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuriy","family":"Prylutskyy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Scharff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"16765_CR1","doi-asserted-by":"publisher","first-page":"2230","DOI":"10.1038\/s41386-021-01069-4","volume":"46","author":"ND Volkow","year":"2021","unstructured":"Volkow, N. 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