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Despite its growing clinical importance, the genetic determinants of its pathogenicity remain poorly understood. This study aimed to identify essential genes in <jats:italic>S.<\/jats:italic>\u00a0<jats:italic>anginosus<\/jats:italic> 980\/01, a bloodstream isolate, under nutrient-rich laboratory conditions using a transposon mutagenesis combined with Transposon-Directed Insertion Site Sequencing (TraDIS). A mutant library was generated using the IS<jats:italic>S1<\/jats:italic> transposon delivered via the thermosensitive plasmid pGh9:IS<jats:italic>S1<\/jats:italic>. Following transposition, insertions were mapped using Illumina sequencing and subsequently analyzed. Essential genes were identified based on the absence of insertions and statistical filtering. The library exhibited 98% genome saturation with over 130,000 unique insertion sites. Among 1825 genes, 348 (19.1%) were essential, 1446 non-essential, and 30 non-conclusive. Comparative analyses were performed with <jats:italic>S.<\/jats:italic>\u00a0<jats:italic>pyogenes<\/jats:italic> MGAS5005 and <jats:italic>S.<\/jats:italic>\u00a0<jats:italic>agalactiae<\/jats:italic> A909. Similarly to the latter, essential genes were enriched in functions related to translation, transcription, and cell wall biosynthesis. However, 40 genes uniquely essential to <jats:italic>S.\u00a0anginosus<\/jats:italic> 980\/01 were identified, suggesting unique survival strategies in <jats:italic>S.<\/jats:italic>\u00a0<jats:italic>anginosus<\/jats:italic>. This study presents the first genome-wide identification of essential genes for <jats:italic>S.<\/jats:italic>\u00a0<jats:italic>anginosus<\/jats:italic> 980\/01, highlighting conserved and unique essential genes. These findings provide a basis for understanding its physiology and key genetic determinants of bacterial viability, and may help to uncover the pathogenic potential of <jats:italic>S.\u00a0anginosus<\/jats:italic> in future studies.<\/jats:p>","DOI":"10.1038\/s41598-025-18002-0","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:46:01Z","timestamp":1758807961000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide identification of essential genes in the invasive Streptococcus anginosus strain"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3846-7234","authenticated-orcid":false,"given":"Aleksandra","family":"Kury\u0142ek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3800-2557","authenticated-orcid":false,"given":"Jan","family":"Gawor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7097-4025","authenticated-orcid":false,"given":"Karolina","family":"\u017buchniewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9941-6692","authenticated-orcid":false,"given":"Robert","family":"Gromadka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3452-8167","authenticated-orcid":false,"given":"Izabela","family":"Kern-Zdanowicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"18002_CR1","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1128\/jcm.30.1.243-244.1992","volume":"30","author":"RA Whiley","year":"1992","unstructured":"Whiley, R. 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