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This study investigated the tibialis anterior (TA) shear modulus using shear wave elastography (SWE) and studied its relationship with ankle angle, contraction intensity, and joint moment-derived TA force and stress. Fourteen healthy volunteers (seven females, 26.43\u2009\u00b1\u20093.67\u00a0years) participated. SWE from TA, EMG, and ankle joint moment data were collected across ankle angles (\u2212\u200915\u00b0 dorsiflexion to 45\u00b0 plantar flexion) during rest, maximum voluntary contraction (MVC), and isometric submaximal contractions. TA muscle length, passive ankle joint moment, and TA passive shear modulus increased with increasing plantar flexion (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). During MVC, ankle joint moment peaked at 15\u00b0 (50.13\u00a0Nm\u2009\u00b1\u200915.54\u00a0Nm) whereas shear modulus remained unchanged (122.96\u2009\u00b1\u20099.87\u00a0kPa) across muscle lengths (<jats:italic>p<\/jats:italic>\u2009=\u20090.068). SWE reflected contractions at 25%, 50%, and 75% MVC (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). TA force estimates peaked between 15\u00b0 and 30\u00b0, with no significant decrease beyond this range. While SWE captured length-dependent passive properties and contraction intensity changes, the shear modulus at MVC (a stiffness measure obtained from SWE) did not align with the tangent modulus (derived from joint-moment-based force\u2013length characteristics). Emphasizing the need for validation, SWE could serve as a valuable tool for understanding muscle mechanics and muscles\u2019 roles in joint dynamics.<\/jats:p>","DOI":"10.1038\/s41598-025-18292-4","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T09:12:34Z","timestamp":1757668354000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In vivo tibialis anterior muscle mechanics through force estimation using ankle joint moment and shear wave elastography"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7345-0772","authenticated-orcid":false,"given":"Cemre Su","family":"Kaya Keles","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Hiller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2405-863X","authenticated-orcid":false,"given":"Manuela","family":"Zimmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5634-3913","authenticated-orcid":false,"given":"Filiz","family":"Ates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"18292_CR1","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1016\/S0268-0033(99)00018-2","volume":"14","author":"CN Maganaris","year":"1999","unstructured":"Maganaris, C. 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