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Two medicinal plants,\n                    <jats:italic>Rorippa islandica<\/jats:italic>\n                    and\n                    <jats:italic>Carrichtera annua<\/jats:italic>\n                    (family Brassicaceae), collected from the NorthWestern Coastal region (Marsa Matrouh, Egypt), were investigated to determine their bioactive constituents in ethanol crude extracts using GC-MS and HPLC techniques, and to evaluate their in vitro antifungal activity against five pathogenic fungi. GC-MS analysis revealed the presence of 50 and 54 compounds in\n                    <jats:italic>R. islandica<\/jats:italic>\n                    and\n                    <jats:italic>C. annua<\/jats:italic>\n                    , respectively. The primary compound in\n                    <jats:italic>R. islandica<\/jats:italic>\n                    was 13-Docosenamide (20.54%), while in\n                    <jats:italic>C. annua<\/jats:italic>\n                    it was 2-Hydroxy-1-(hydroxymethyl) ethyl stearate (9.74%). HPLC identified 19 and 18 phenolic compounds in\n                    <jats:italic>R. islandica<\/jats:italic>\n                    and\n                    <jats:italic>C. annua<\/jats:italic>\n                    , respectively, with gallic acid as the predominant compound in both plants (3417.72\u00a0\u00b5g\/g and 3733.98\u00a0\u00b5g\/g, respectively). Both plant extracts exhibited promising antifungal activity. For\n                    <jats:italic>R. islandica<\/jats:italic>\n                    , the most potent effect at 10\u00a0mg\/ml was observed with the 70% ethanol successive extract against\n                    <jats:italic>Colletotrichum gloeosporioides<\/jats:italic>\n                    (inhibition zone 29\u2009\u00b1\u20090.3\u00a0mm; MIC 7.8\u00a0\u00b5g\/ml; MFC 15.62\u00a0\u00b5g\/ml). For\n                    <jats:italic>C. annua<\/jats:italic>\n                    , the most potent effect at 10\u00a0mg\/ml was from the total extract against\n                    <jats:italic>Curvularia lunata<\/jats:italic>\n                    (inhibition zone 35\u2009\u00b1\u20090.1\u00a0mm; MIC 1.97\u00a0\u00b5g\/ml; MFC 3.9\u00a0\u00b5g\/ml). A significant reduction in ergosterol content was observed in the total and successive ethanol (96% and 70%) fractions of both plants, with the highest reduction in\n                    <jats:italic>C. lunata<\/jats:italic>\n                    and\n                    <jats:italic>C. gloeosporioides<\/jats:italic>\n                    treated with\n                    <jats:italic>C. annua<\/jats:italic>\n                    extract (49.93% and 47.7%, respectively), and in\n                    <jats:italic>P. glabrum<\/jats:italic>\n                    and\n                    <jats:italic>C. gloeosporioides<\/jats:italic>\n                    treated with\n                    <jats:italic>R. islandica<\/jats:italic>\n                    extract (47.2% and 42.58%, respectively). Morphological changes induced by the total and ethanol (96% and 70%) extracts of both plants were examined using AFM.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-18681-9","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T13:43:50Z","timestamp":1759844630000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antimicrobial activity, ergosterol content and phytochemical screening of Rorippa Islandica (Oeder ex Murr.) and Carrichtera annua (L.)"],"prefix":"10.1038","volume":"15","author":[{"given":"Heba","family":"Yehia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa A.","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samah A.","family":"Donia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naziha M.","family":"Hassanein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enas","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,7]]},"reference":[{"key":"18681_CR1","doi-asserted-by":"publisher","unstructured":"Villaverde, J. 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