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Here, we present a refined surgical model that combines structured intraoperative modifications with tailored mechanical ventilation strategies to ensure procedural consistency and biologic fidelity. Key interventions\u2014such as a custom leak-proof endotracheal tube, a temporally controlled reperfusion maneuver, and an optimized anastomotic sequence\u2014collectively addressed critical challenges including airway obstruction, graft impaired perfusion, and venous fragility. The protocol demonstrated a high procedural success rate and reproducible long-term survival, with histopathological analysis confirming progressive chronic remodeling and diminished acute rejection. Importantly, the technique demonstrated features that facilitate rapid acquisition, supported by a short learning curve, and was implemented using widely accessible clinical-grade materials. Together, these attributes establish a robust and scalable platform for investigating chronic lung allograft dysfunction and other long-term immunologic phenomena.<\/jats:p>","DOI":"10.1038\/s41598-025-19711-2","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T14:51:19Z","timestamp":1760453479000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Refined cuff technique minimizes surgical complexity in rat lung transplantation and improves outcomes"],"prefix":"10.1038","volume":"15","author":[{"given":"Mehmet Furkan","family":"Sahin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunes","family":"Esendagl\u0131","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma","family":"Y\u0131ld\u0131r\u0131m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erdal","family":"Yekeler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Banu","family":"K\u0131l\u0131caslan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"19711_CR1","first-page":"1018","volume":"6","author":"BA Whitson","year":"2014","unstructured":"Whitson, B. 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All animal experiments were approved by the Institutional Animal Ethics Committee of Kobay Experimental Animals Laboratory (Ankara, T\u00fcrkiye), on April 3, 2024 (Approval No: 24\u2013713), in accordance with the Helsinki Guidelines for Animal Welfare and the Turkish Veterinary Deontology Regulation (Law No. 6343\/2).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Statement"}},{"value":"The authors have completed the ARRIVE reporting checklist.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Reporting checklist"}}],"article-number":"35876"}}