{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T21:16:21Z","timestamp":1776806181314,"version":"3.51.2"},"reference-count":84,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Youth Project of Shandong Provincial Natural Science Foundation","award":["ZR2022QH322"],"award-info":[{"award-number":["ZR2022QH322"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82472366"],"award-info":[{"award-number":["82472366"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-20037-2","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:21:45Z","timestamp":1760527305000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring the regulatory mechanisms of paraptosis-related prognostic genes in gastric cancer using single-cell sequencing and transcriptome analysis"],"prefix":"10.1038","volume":"15","author":[{"given":"Zhengliang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linchuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanxin","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangchang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xushuo","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dexu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunlu","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiankang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangyong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"20037_CR1","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1016\/S0140-6736(20)31288-5","volume":"396","author":"EC Smyth","year":"2020","unstructured":"Smyth, E. C., Nilsson, M., Grabsch, H. I., van Grieken, N. C. & Lordick, F. Gastric cancer. Lancet 396, 635\u2013648 (2020).","journal-title":"Lancet"},{"key":"20037_CR2","first-page":"209","volume":"71","author":"H Sung","year":"2021","unstructured":"Sung, H. et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 71, 209\u2013249 (2021).","journal-title":"CA Cancer J. Clin."},{"key":"20037_CR3","doi-asserted-by":"publisher","first-page":"2452","DOI":"10.3748\/wjg.v29.i16.2452","volume":"29","author":"WJ Yang","year":"2023","unstructured":"Yang, W. J. et al. Updates on global epidemiology, risk and prognostic factors of gastric cancer. World J. Gastroenterol. 29, 2452\u20132468 (2023).","journal-title":"World J. Gastroenterol."},{"key":"20037_CR4","doi-asserted-by":"crossref","unstructured":"Machlowska, J., Baj, J., Sitarz, M., Maciejewski, R. & Sitarz, R. Gastric cancer: epidemiology, risk factors, classification, genomic characteristics and treatment strategies. Int J. Mol. Sci 21 (2020).","DOI":"10.3390\/ijms21114012"},{"key":"20037_CR5","doi-asserted-by":"publisher","first-page":"752346","DOI":"10.3389\/fcell.2021.752346","volume":"9","author":"SG Hoft","year":"2021","unstructured":"Hoft, S. G., Noto, C. N. & DiPaolo, R. J. Two distinct etiologies of gastric cancer: infection and autoimmunity. Front. Cell. Dev. Biol. 9, 752346 (2021).","journal-title":"Front. Cell. Dev. Biol."},{"key":"20037_CR6","doi-asserted-by":"crossref","unstructured":"Comprehensive molecular characterization of gastric adenocarcinoma. Nature 513, 202\u2013209 (2014).","DOI":"10.1038\/nature13480"},{"key":"20037_CR7","doi-asserted-by":"publisher","first-page":"e18761429318398","DOI":"10.2174\/0118761429318398240918063450","volume":"17","author":"X Zhang","year":"2024","unstructured":"Zhang, X. et al. Upregulation of LncRNA WT1-AS inhibits tumor growth and promotes autophagy in gastric cancer via suppression of PI3K\/Akt\/mTOR pathway. Curr. Mol. Pharmacol. 17, e18761429318398 (2024).","journal-title":"Curr. Mol. Pharmacol."},{"key":"20037_CR8","doi-asserted-by":"crossref","unstructured":"Japanese gastric cancer treatment guidelines 2018 (5th edition). Gastric Cancer 24, 1\u201321. (2021).","DOI":"10.1007\/s10120-020-01042-y"},{"key":"20037_CR9","first-page":"712","volume":"16","author":"J Song","year":"2023","unstructured":"Song, J. et al. Myristicin suppresses gastric cancer growth via targeting the EGFR\/ ERK signaling pathway. Curr. Mol. Pharmacol. 16, 712\u2013724 (2023).","journal-title":"Curr. Mol. Pharmacol."},{"key":"20037_CR10","doi-asserted-by":"publisher","first-page":"e18761429322420","DOI":"10.2174\/0118761429322420241112051105","volume":"17","author":"S Fatima","year":"2024","unstructured":"Fatima, S. et al. Exploring the Pharmacological mechanisms of P-hydroxylcinnamaldehyde for treating gastric cancer: A Pharmacological perspective with experimental confirmation. Curr. Mol. Pharmacol. 17, e18761429322420 (2024).","journal-title":"Curr. Mol. Pharmacol."},{"key":"20037_CR11","doi-asserted-by":"publisher","first-page":"453","DOI":"10.2174\/0115734129322567240821052326","volume":"20","author":"VC Chandni","year":"2024","unstructured":"Chandni, V. C., Namira, T. M., Diksha, V. S. & Utkarsh, B. K. Vibrational spectrophotometry: A comprehensive review on the diagnosis of gastric and liver cancer. Curr. Pharm. Anal. 20, 453\u2013465 (2024).","journal-title":"Curr. Pharm. Anal."},{"key":"20037_CR12","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1038\/s41401-023-01159-7","volume":"45","author":"CC Xu","year":"2024","unstructured":"Xu, C. C. et al. Paraptosis: a non-classical paradigm of cell death for cancer therapy. Acta Pharmacol. Sin. 45, 223\u2013237 (2024).","journal-title":"Acta Pharmacol. Sin"},{"key":"20037_CR13","doi-asserted-by":"crossref","unstructured":"Kunst, C. et al. Paraptosis-A distinct pathway to cell death. Int J. Mol. Sci 25 (2024).","DOI":"10.3390\/ijms252111478"},{"key":"20037_CR14","doi-asserted-by":"publisher","first-page":"607844","DOI":"10.3389\/fcell.2020.607844","volume":"8","author":"E Kim","year":"2020","unstructured":"Kim, E., Lee, D. M., Seo, M. J., Lee, H. J. & Choi, K. S. Intracellular Ca(2 +) Imbalance Critically Contributes to Paraptosis. Front. Cell. Dev. Biol. 8, 607844 (2020).","journal-title":"Front. Cell. Dev. Biol."},{"key":"20037_CR15","doi-asserted-by":"publisher","first-page":"9972","DOI":"10.1021\/acsnano.2c11964","volume":"17","author":"RR Zheng","year":"2023","unstructured":"Zheng, R. R. et al. Paraptosis inducer to effectively trigger Immunogenic cell death for metastatic tumor immunotherapy with IDO Inhibition. ACS Nano. 17, 9972\u20139986 (2023).","journal-title":"ACS Nano"},{"key":"20037_CR16","doi-asserted-by":"publisher","first-page":"1159409","DOI":"10.3389\/fphar.2023.1159409","volume":"14","author":"S Hanson","year":"2023","unstructured":"Hanson, S. et al. Paraptosis: a unique cell death mode for targeting cancer. Front. Pharmacol. 14, 1159409 (2023).","journal-title":"Front. Pharmacol."},{"key":"20037_CR17","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/s41417-023-00722-y","volume":"31","author":"F Chen","year":"2024","unstructured":"Chen, F. et al. Targeting paraptosis in cancer: opportunities and challenges. Cancer Gene Ther. 31, 349\u2013363 (2024).","journal-title":"Cancer Gene Ther."},{"key":"20037_CR18","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.pharmthera.2016.01.003","volume":"162","author":"D Lee","year":"2016","unstructured":"Lee, D., Kim, I. Y., Saha, S. & Choi, K. S. Paraptosis in the anti-cancer arsenal of natural products. Pharmacol. Ther. 162, 120\u2013133 (2016).","journal-title":"Pharmacol. Ther."},{"key":"20037_CR19","doi-asserted-by":"crossref","unstructured":"Zhuo, Y. & Song, Y. Prognostic and immunological implications of paraptosis-related genes in lung adenocarcinoma: comprehensive analysis and functional verification of hub gene. Environ Toxicol (2024).","DOI":"10.1002\/tox.24185"},{"key":"20037_CR20","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1007\/s11033-024-09553-y","volume":"51","author":"L Duhan","year":"2024","unstructured":"Duhan, L. et al. Single-cell transcriptomics: background, technologies, applications, and challenges. Mol. Biol. Rep. 51, 600 (2024).","journal-title":"Mol. Biol. Rep."},{"key":"20037_CR21","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1186\/s13045-021-01105-2","volume":"14","author":"Y Lei","year":"2021","unstructured":"Lei, Y. et al. Applications of single-cell sequencing in cancer research: progress and perspectives. J. Hematol. Oncol. 14, 91 (2021).","journal-title":"J. Hematol. Oncol."},{"key":"20037_CR22","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/j.molcel.2020.09.025","volume":"80","author":"V Zachariadis","year":"2020","unstructured":"Zachariadis, V., Cheng, H., Andrews, N. & Enge, M. A. Highly scalable method for joint Whole-Genome sequencing and Gene-Expression profiling of single cells. Mol. Cell. 80, 541\u2013553e5 (2020).","journal-title":"Mol. Cell."},{"key":"20037_CR23","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1186\/s12967-023-04468-x","volume":"21","author":"H Qin","year":"2023","unstructured":"Qin, H. et al. Integrated machine learning survival framework develops a prognostic model based on inter-crosstalk definition of mitochondrial function and cell death patterns in a large multicenter cohort for lower-grade glioma. J. Transl Med. 21, 588 (2023).","journal-title":"J. Transl Med."},{"key":"20037_CR24","doi-asserted-by":"publisher","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","volume":"184","author":"Y Hao","year":"2021","unstructured":"Hao, Y. et al. Integrated analysis of multimodal single-cell data. Cell 184, 3573\u20133587e29 (2021).","journal-title":"Cell"},{"key":"20037_CR25","doi-asserted-by":"publisher","first-page":"e0325520","DOI":"10.1371\/journal.pone.0325520","volume":"20","author":"J Chu","year":"2025","unstructured":"Chu, J. et al. Utilizing Multi-omics analysis to elucidate the role of mitochondrial gene defects in gastric cancer progression. PLoS One. 20, e0325520 (2025).","journal-title":"PLoS One"},{"key":"20037_CR26","doi-asserted-by":"crossref","unstructured":"Zhang, J. et al. Machine learning integration with Single-Cell transcriptome sequencing datasets reveals the impact of Tumor-Associated neutrophils on the immune microenvironment and immunotherapy outcomes in gastric cancer. Int J. Mol. Sci 25 (2024).","DOI":"10.3390\/ijms252312715"},{"key":"20037_CR27","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1186\/s12859-020-3538-2","volume":"21","author":"HA Ekiz","year":"2020","unstructured":"Ekiz, H. A., Conley, C. J., Stephens, W. Z. & O\u2019Connell, R. M. CIPR: a web-based r\/shiny app and R package to annotate cell clusters in single cell RNA sequencing experiments. BMC Bioinform. 21, 191 (2020).","journal-title":"BMC Bioinform."},{"key":"20037_CR28","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1186\/s13059-017-1349-1","volume":"18","author":"D Aran","year":"2017","unstructured":"Aran, D., Hu, Z. & Butte, A. J. xCell: digitally portraying the tissue cellular heterogeneity landscape. Genome Biol. 18, 220 (2017).","journal-title":"Genome Biol."},{"key":"20037_CR29","doi-asserted-by":"publisher","first-page":"100980","DOI":"10.1016\/j.neo.2024.100980","volume":"50","author":"L Wang","year":"2024","unstructured":"Wang, L. et al. Single cell analysis unveils the commonality and heterogeneity between nasopharyngeal and oropharyngeal carcinoma. Neoplasia 50, 100980 (2024).","journal-title":"Neoplasia"},{"key":"20037_CR30","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love, M. I., Huber, W. & Anders, S. Moderated Estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).","journal-title":"Genome Biol."},{"key":"20037_CR31","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1080\/07357907.2024.2368233","volume":"42","author":"D Liu","year":"2024","unstructured":"Liu, D. et al. A bioinformatic analysis of gut microbiota related with immune cell infiltration in colorectal cancer. Cancer Invest. 42, 491\u2013499 (2024).","journal-title":"Cancer Invest."},{"key":"20037_CR32","doi-asserted-by":"crossref","unstructured":"Terrematte, P. et al. A novel machine learning 13-Gene signature: improving risk analysis and survival prediction for clear cell renal cell carcinoma patients. Cancers (Basel) 14 (2022).","DOI":"10.3390\/cancers14092111"},{"key":"20037_CR33","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1186\/1471-2105-9-559","volume":"9","author":"P Langfelder","year":"2008","unstructured":"Langfelder, P. & Horvath, S. WGCNA: an R package for weighted correlation network analysis. BMC Bioinform. 9, 559 (2008).","journal-title":"BMC Bioinform."},{"key":"20037_CR34","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1093\/nar\/28.1.27","volume":"28","author":"M Kanehisa","year":"2000","unstructured":"Kanehisa, M. & Goto, S. KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28, 27\u201330 (2000).","journal-title":"Nucleic Acids Res."},{"key":"20037_CR35","doi-asserted-by":"publisher","first-page":"D672","DOI":"10.1093\/nar\/gkae909","volume":"53","author":"M Kanehisa","year":"2025","unstructured":"Kanehisa, M., Furumichi, M., Sato, Y., Matsuura, Y. & Ishiguro-Watanabe, M. KEGG: biological systems database as a model of the real world. Nucleic Acids Res. 53, D672\u2013d677 (2025).","journal-title":"Nucleic Acids Res."},{"key":"20037_CR36","doi-asserted-by":"publisher","first-page":"1947","DOI":"10.1002\/pro.3715","volume":"28","author":"M Kanehisa","year":"2019","unstructured":"Kanehisa, M. Toward Understanding the origin and evolution of cellular organisms. Protein Sci. 28, 1947\u20131951 (2019).","journal-title":"Protein Sci."},{"key":"20037_CR37","doi-asserted-by":"crossref","unstructured":"Li, H. et al. Identification of Age-Related characteristic genes involved in severe COVID-19 infection among elderly patients using machine learning and immune cell infiltration analysis. Biochem Genet (2024).","DOI":"10.21203\/rs.3.rs-2909554\/v1"},{"key":"20037_CR38","doi-asserted-by":"publisher","first-page":"1198","DOI":"10.1186\/s12885-020-07657-9","volume":"20","author":"C Li","year":"2020","unstructured":"Li, C., Long, Q., Zhang, D., Li, J. & Zhang, X. Identification of a four-gene panel predicting overall survival for lung adenocarcinoma. BMC Cancer. 20, 1198 (2020).","journal-title":"BMC Cancer"},{"key":"20037_CR39","doi-asserted-by":"publisher","first-page":"1121485","DOI":"10.3389\/fonc.2023.1121485","volume":"13","author":"Y Xie","year":"2023","unstructured":"Xie, Y. et al. Development and validation of a CECT-based radiomics model for predicting IL1B expression and prognosis of head and neck squamous cell carcinoma. Front. Oncol. 13, 1121485 (2023).","journal-title":"Front. Oncol."},{"key":"20037_CR40","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v033.i01","volume":"33","author":"J Friedman","year":"2010","unstructured":"Friedman, J., Hastie, T. & Tibshirani, R. Regularization paths for generalized linear models via coordinate descent. J. Stat. Softw. 33, 1\u201322 (2010).","journal-title":"J. Stat. Softw."},{"key":"20037_CR41","doi-asserted-by":"crossref","unstructured":"Ciarmiello, A. et al. Multivariable risk modelling and survival analysis with machine learning in SARS-CoV-2 infection. J Clin. Med 12 (2023).","DOI":"10.1101\/2023.06.22.23291773"},{"key":"20037_CR42","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/1471-2105-12-77","volume":"12","author":"X Robin","year":"2011","unstructured":"Robin, X. et al. pROC: an open-source package for R and S\u2009+\u2009to analyze and compare ROC curves. BMC Bioinform. 12, 77 (2011).","journal-title":"BMC Bioinform."},{"key":"20037_CR43","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1186\/s12967-020-02374-0","volume":"18","author":"J Liu","year":"2020","unstructured":"Liu, J. et al. Neutrophil-to-lymphocyte ratio predicts critical illness patients with 2019 coronavirus disease in the early stage. J. Transl Med. 18, 206 (2020).","journal-title":"J. Transl Med."},{"key":"20037_CR44","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/j.pld.2022.05.005","volume":"44","author":"Y Jin","year":"2022","unstructured":"Jin, Y. & Qian, H. V. PhyloMaker2: an updated and enlarged R package that can generate very large phylogenies for vascular plants. Plant. Divers. 44, 335\u2013339 (2022).","journal-title":"Plant. Divers."},{"key":"20037_CR45","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","volume":"13","author":"P Shannon","year":"2003","unstructured":"Shannon, P. et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 13, 2498\u20132504 (2003).","journal-title":"Genome Res."},{"key":"20037_CR46","doi-asserted-by":"publisher","first-page":"2756","DOI":"10.1002\/bit.28431","volume":"120","author":"S Azadian","year":"2023","unstructured":"Azadian, S. et al. Reconstructing the cell-cell interaction network among mouse immune cells. Biotechnol. Bioeng. 120, 2756\u20132764 (2023).","journal-title":"Biotechnol. Bioeng."},{"key":"20037_CR47","doi-asserted-by":"publisher","first-page":"1286087","DOI":"10.3389\/fimmu.2023.1286087","volume":"14","author":"Z Zheng","year":"2023","unstructured":"Zheng, Z., Zhou, Y., Song, Y., Ying, P. & Tan, X. Genetic and immunological insights into COVID-19 with acute myocardial infarction: integrated analysis of Mendelian randomization, transcriptomics, and clinical samples. Front. Immunol. 14, 1286087 (2023).","journal-title":"Front. Immunol."},{"key":"20037_CR48","doi-asserted-by":"crossref","unstructured":"Joo, E. H. et al. Migratory tumor cells cooperate with cancer associated fibroblasts in hormone Receptor-Positive and HER2-Negative breast cancer. Int J. Mol. Sci 25 (2024).","DOI":"10.3390\/ijms25115876"},{"key":"20037_CR49","doi-asserted-by":"crossref","unstructured":"Hsiao, B. Y. et al. Human costars family protein ABRACL modulates actin dynamics and cell migration and associates with tumorigenic growth. Int J. Mol. Sci 22 (2021).","DOI":"10.3390\/ijms22042037"},{"key":"20037_CR50","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1089\/gtmb.2018.0195","volume":"23","author":"D Wang","year":"2019","unstructured":"Wang, D., Liu, H., Ren, C. & Wang, L. High expression of ABRACL is associated with tumorigenesis and affects clinical outcome in gastric cancer. Genet. Test. Mol. Biomarkers. 23, 91\u201397 (2019).","journal-title":"Genet. Test. Mol. Biomarkers"},{"key":"20037_CR51","unstructured":"Guo, K. & Gao, X. ABRACL upregulated by transcription factor CBX4 promotes proliferation and migration and inhibits the apoptosis of gastric cancer cells. Histol Histopathol, 18812 (2024)."},{"key":"20037_CR52","doi-asserted-by":"publisher","first-page":"986990","DOI":"10.3389\/fonc.2022.986990","volume":"12","author":"W Lv","year":"2022","unstructured":"Lv, W., Shi, L., Pan, J. & Wang, S. Comprehensive prognostic and immunological analysis of CCT2 in pan-cancer. Front. Oncol. 12, 986990 (2022).","journal-title":"Front. Oncol."},{"key":"20037_CR53","doi-asserted-by":"publisher","first-page":"101423","DOI":"10.1016\/j.tranon.2022.101423","volume":"20","author":"X Ma","year":"2022","unstructured":"Ma, X. et al. TSPAN31 regulates the proliferation, migration, and apoptosis of gastric cancer cells through the METTL1\/CCT2 pathway. Transl Oncol. 20, 101423 (2022).","journal-title":"Transl Oncol."},{"key":"20037_CR54","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/s00262-008-0543-0","volume":"58","author":"D Rodriguez-Pinto","year":"2009","unstructured":"Rodriguez-Pinto, D. et al. Identification of novel tumor antigens with patient-derived immune-selected antibodies. Cancer Immunol. Immunother. 58, 221\u2013234 (2009).","journal-title":"Cancer Immunol. Immunother"},{"key":"20037_CR55","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1016\/j.jgg.2023.01.008","volume":"50","author":"B Gong","year":"2023","unstructured":"Gong, B. et al. Epigenetic and transcriptional activation of the secretory kinase FAM20C as an oncogene in glioma. J. Genet. Genomics. 50, 422\u2013433 (2023).","journal-title":"J. Genet. Genomics"},{"key":"20037_CR56","doi-asserted-by":"crossref","unstructured":"Audia, S. et al. RANBP1 (RAN binding protein 1): the missing genetic piece in cancer pathophysiology and other complex diseases. Cancers (Basel) 15 (2023).","DOI":"10.3390\/cancers15020486"},{"key":"20037_CR57","doi-asserted-by":"crossref","unstructured":"Kahm, Y. J., Kim, I. G. & Kim, R. K. RanBP1: A potential therapeutic target for cancer stem cells in lung cancer and glioma. Int J. Mol. Sci 24 (2023).","DOI":"10.3390\/ijms24076855"},{"key":"20037_CR58","first-page":"1738","volume":"12","author":"P Xu","year":"2019","unstructured":"Xu, P., Shen, P., Jin, Y., Wang, L. & Wu, W. Epithelioid inflammatory myofibroblastic sarcoma of stomach: diagnostic pitfalls and clinical characteristics. Int. J. Clin. Exp. Pathol. 12, 1738\u20131744 (2019).","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"20037_CR59","doi-asserted-by":"publisher","first-page":"8862228","DOI":"10.1155\/2020\/8862228","volume":"2020","author":"JF Wang","year":"2020","unstructured":"Wang, J. F. et al. Expression and prognostic analysis of integrins in gastric cancer. J Oncol 2020, 8862228 (2020).","journal-title":"J Oncol"},{"key":"20037_CR60","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1186\/s12935-020-01344-2","volume":"20","author":"T Zhu","year":"2020","unstructured":"Zhu, T. et al. The prognostic value of ITGA and ITGB superfamily members in patients with high grade serous ovarian cancer. Cancer Cell. Int. 20, 257 (2020).","journal-title":"Cancer Cell. Int."},{"key":"20037_CR61","first-page":"923","volume":"42","author":"YZ Gong","year":"2019","unstructured":"Gong, Y. Z. et al. Diagnostic and prognostic values of integrin \u03b1 subfamily mRNA expression in colon adenocarcinoma. Oncol. Rep. 42, 923\u2013936 (2019).","journal-title":"Oncol. Rep."},{"key":"20037_CR62","doi-asserted-by":"publisher","first-page":"e0292452","DOI":"10.1371\/journal.pone.0292452","volume":"18","author":"L Xie","year":"2023","unstructured":"Xie, L., Xiao, W., Fang, H. & Liu, G. RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma. PLoS One. 18, e0292452 (2023).","journal-title":"PLoS One"},{"key":"20037_CR63","doi-asserted-by":"publisher","first-page":"4909544","DOI":"10.1155\/2022\/4909544","volume":"2022","author":"R Dong","year":"2022","unstructured":"Dong, R. et al. Models for predicting response to immunotherapy and prognosis in patients with gastric cancer: DNA damage response genes. Biomed Res Int 2022, 4909544 (2022).","journal-title":"Biomed Res Int"},{"key":"20037_CR64","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1186\/s12864-024-10359-2","volume":"25","author":"Y Yang","year":"2024","unstructured":"Yang, Y. & Zhang, J. Ascites-derived hsa-miR-181a-5p serves as a prognostic marker for gastric cancer-associated malignant Ascites. BMC Genom. 25, 628 (2024).","journal-title":"BMC Genom."},{"key":"20037_CR65","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1038\/s41586-022-05374-w","volume":"611","author":"M Balood","year":"2022","unstructured":"Balood, M. et al. Nociceptor neurons affect cancer immunosurveillance. Nature 611, 405\u2013412 (2022).","journal-title":"Nature"},{"key":"20037_CR66","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1007\/s10238-022-00885-2","volume":"23","author":"Y Zhang","year":"2023","unstructured":"Zhang, Y., Lv, X., Chen, L. & Liu, Y. The role and function of CLU in cancer biology and therapy. Clin. Exp. Med. 23, 1375\u20131391 (2023).","journal-title":"Clin. Exp. Med."},{"key":"20037_CR67","doi-asserted-by":"publisher","first-page":"2188","DOI":"10.1016\/j.immuni.2023.09.011","volume":"56","author":"I Mellman","year":"2023","unstructured":"Mellman, I., Chen, D. S., Powles, T. & Turley, S. J. The cancer-immunity cycle: indication, genotype, and immunotype. Immunity 56, 2188\u20132205 (2023).","journal-title":"Immunity"},{"key":"20037_CR68","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/imr.12772","volume":"290","author":"SS Chandran","year":"2019","unstructured":"Chandran, S. S. & Klebanoff, C. A. T cell receptor-based cancer immunotherapy: emerging efficacy and pathways of resistance. Immunol. Rev. 290, 127\u2013147 (2019).","journal-title":"Immunol. Rev."},{"key":"20037_CR69","doi-asserted-by":"publisher","first-page":"715234","DOI":"10.3389\/fimmu.2021.715234","volume":"12","author":"JS Dolina","year":"2021","unstructured":"Dolina, J. S., Van Braeckel-Budimir, N., Thomas, G. D. & Salek-Ardakani, S. CD8(+) T cell exhaustion in cancer. Front. Immunol. 12, 715234 (2021).","journal-title":"Front. Immunol."},{"key":"20037_CR70","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1002\/JLB.MR0318-097R","volume":"105","author":"D Chraa","year":"2019","unstructured":"Chraa, D., Naim, A., Olive, D. & Badou, A. T lymphocyte subsets in cancer immunity: friends or foes. J. Leukoc. Biol. 105, 243\u2013255 (2019).","journal-title":"J. Leukoc. Biol."},{"key":"20037_CR71","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1084\/jem.135.4.890","volume":"135","author":"P Golstein","year":"1972","unstructured":"Golstein, P., Wigzell, H., Blomgren, H. & Svedmyr, E. A. Cells mediating specific in vitro cytotoxicity. II. Probable autonomy of thymus-processed lymphocytes (T cells) for the killing of allogeneic target cells. J. Exp. Med. 135, 890\u2013906 (1972).","journal-title":"J. Exp. Med."},{"key":"20037_CR72","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1038\/nm.1982","volume":"15","author":"L Gattinoni","year":"2009","unstructured":"Gattinoni, L. et al. Wnt signaling arrests effector T cell differentiation and generates CD8\u2009+\u2009memory stem cells. Nat. Med. 15, 808\u2013813 (2009).","journal-title":"Nat. Med."},{"key":"20037_CR73","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1097\/CJI.0b013e3181acec1e","volume":"32","author":"K Sasaki","year":"2009","unstructured":"Sasaki, K. et al. IL-4 suppresses very late antigen-4 expression which is required for therapeutic Th1 T-cell trafficking into tumors. J. Immunother. 32, 793\u2013802 (2009).","journal-title":"J. Immunother"},{"key":"20037_CR74","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1002\/jso.21725","volume":"102","author":"H Ubukata","year":"2010","unstructured":"Ubukata, H. et al. Evaluations of interferon-\u03b3\/interleukin-4 ratio and neutrophil\/lymphocyte ratio as prognostic indicators in gastric cancer patients. J. Surg. Oncol. 102, 742\u2013747 (2010).","journal-title":"J. Surg. Oncol."},{"key":"20037_CR75","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.cyto.2007.06.002","volume":"38","author":"Y Zhou","year":"2007","unstructured":"Zhou, Y., Toh, M. L., Zrioual, S. & Miossec, P. IL-17A versus IL-17F induced intracellular signal transduction pathways and modulation by IL-17RA and IL-17RC RNA interference in AGS gastric adenocarcinoma cells. Cytokine 38, 157\u2013164 (2007).","journal-title":"Cytokine"},{"key":"20037_CR76","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1038\/s41575-019-0115-0","volume":"16","author":"H Kobayashi","year":"2019","unstructured":"Kobayashi, H. et al. Cancer-associated fibroblasts in Gastrointestinal cancer. Nat. Rev. Gastroenterol. Hepatol. 16, 282\u2013295 (2019).","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"20037_CR77","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1152\/physrev.00048.2019","volume":"101","author":"G Biffi","year":"2021","unstructured":"Biffi, G. & Tuveson, D. A. Diversity and biology of Cancer-Associated fibroblasts. Physiol. Rev. 101, 147\u2013176 (2021).","journal-title":"Physiol. Rev."},{"key":"20037_CR78","doi-asserted-by":"publisher","first-page":"4294","DOI":"10.1038\/s41467-023-39762-1","volume":"14","author":"L Cords","year":"2023","unstructured":"Cords, L. et al. Cancer-associated fibroblast classification in single-cell and Spatial proteomics data. Nat. Commun. 14, 4294 (2023).","journal-title":"Nat. Commun."},{"key":"20037_CR79","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1158\/2159-8290.CD-21-0683","volume":"12","author":"V Kumar","year":"2022","unstructured":"Kumar, V. et al. Single-Cell atlas of lineage states, tumor microenvironment, and Subtype-Specific expression programs in gastric cancer. Cancer Discov. 12, 670\u2013691 (2022).","journal-title":"Cancer Discov"},{"key":"20037_CR80","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s12943-020-01168-8","volume":"19","author":"H Zhang","year":"2020","unstructured":"Zhang, H. et al. CAF secreted miR-522 suppresses ferroptosis and promotes acquired chemo-resistance in gastric cancer. Mol. Cancer. 19, 43 (2020).","journal-title":"Mol. Cancer"},{"key":"20037_CR81","doi-asserted-by":"publisher","first-page":"1497","DOI":"10.1016\/j.ccell.2024.08.005","volume":"42","author":"I Tirosh","year":"2024","unstructured":"Tirosh, I. & Suva, M. L. Cancer cell states: lessons from ten years of single-cell RNA-sequencing of human tumors. Cancer Cell. 42, 1497\u20131506 (2024).","journal-title":"Cancer Cell."},{"key":"20037_CR82","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1007\/978-1-0716-1307-8_19","volume":"2284","author":"S Slovin","year":"2021","unstructured":"Slovin, S. et al. Single-Cell RNA sequencing analysis: A Step-by-Step overview. Methods Mol. Biol. 2284, 343\u2013365 (2021).","journal-title":"Methods Mol. Biol."},{"key":"20037_CR83","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.neuron.2020.12.010","volume":"109","author":"EJ Armand","year":"2021","unstructured":"Armand, E. J., Li, J., Xie, F., Luo, C. & Mukamel, E. A. Single-Cell sequencing of brain cell transcriptomes and epigenomes. Neuron 109, 11\u201326 (2021).","journal-title":"Neuron"},{"key":"20037_CR84","doi-asserted-by":"publisher","first-page":"1615","DOI":"10.1016\/j.jaci.2020.01.042","volume":"145","author":"H He","year":"2020","unstructured":"He, H. et al. Single-cell transcriptome analysis of human skin identifies novel fibroblast subpopulation and enrichment of immune subsets in atopic dermatitis. J. Allergy Clin. Immunol. 145, 1615\u20131628 (2020).","journal-title":"J. Allergy Clin. Immunol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-20037-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-20037-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-20037-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:04:35Z","timestamp":1760529875000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-20037-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,15]]},"references-count":84,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["20037"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-20037-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,15]]},"assertion":[{"value":"7 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 September 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 October 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The project has been reviewed and approved by the Ethics Review Committee of the First Affiliated Hospital of Shandong First Medical University (2021S1039). The study was conducted in accordance with the principles outlined in the Declaration of Helsinki, ensuring the protection of participants\u2019 rights and well-being throughout the research process.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}],"article-number":"36098"}}