{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T21:54:09Z","timestamp":1787003649589,"version":"build-2736575974"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Petchra Pra Jom Klao Ph.D. Research Scholarship (KMUTT \u2013 NSTDA) from King Mongkut\u2019s University of Technology Thonburi"},{"DOI":"10.13039\/501100004705","name":"Science Faculty, King Mongkut\u2019s University of Technology Thonburi","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004705","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Phramongkutklao College of Medicine"},{"name":"Anandamahidol Foundation"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-21371-1","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T13:48:19Z","timestamp":1761572899000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["LAMP using distance based paper device for quantitative detection of ESBL producing Escherichia coli in UTI patients"],"prefix":"10.1038","volume":"15","author":[{"given":"Natkrittaya","family":"Saengsawang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panthita","family":"Ruang-areerate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saovanee","family":"Leelayoova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathirut","family":"Mungthin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piraporn","family":"Juntanawiwat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patomroek","family":"Hanyanunt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patsanun","family":"Potisuwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Worarachanee","family":"Imjaijitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piyanate","family":"Kesakomol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wijitar","family":"Dungchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toon","family":"Ruang-areerate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"21371_CR1","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1038\/nrurol.2010.190","volume":"7","author":"B Foxman","year":"2010","unstructured":"Foxman, B. The epidemiology of urinary tract infection. Nat. Rev. Urol. 7, 653\u2013660. https:\/\/doi.org\/10.1038\/nrurol.2010.190 (2010).","journal-title":"Nat. Rev. Urol."},{"issue":"Suppl 1","key":"21371_CR2","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/jac\/46.suppl_1.15","volume":"46","author":"G Kahlmeter","year":"2000","unstructured":"Kahlmeter, G. The ECO.SENS project: a prospective, multinational, multicentre epidemiological survey of the prevalence and antimicrobial susceptibility of urinary tract pathogens\u2013interim report. J. Antimicrob. Chemother. 46 (Suppl 1), 15\u201322 (2000). discussion 63\u2009\u2013\u200915.","journal-title":"J. Antimicrob. Chemother."},{"key":"21371_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.idc.2013.09.003","volume":"28","author":"B Foxman","year":"2014","unstructured":"Foxman, B. Urinary tract infection syndromes: occurrence, recurrence, bacteriology, risk factors, and disease burden. Infect. Dis. Clin. North. Am. 28, 1\u201313. https:\/\/doi.org\/10.1016\/j.idc.2013.09.003 (2014).","journal-title":"Infect. Dis. Clin. North. Am."},{"key":"21371_CR4","doi-asserted-by":"publisher","first-page":"138","DOI":"10.7326\/0003-4819-110-2-138","volume":"110","author":"BA Lipsky","year":"1989","unstructured":"Lipsky, B. A. Urinary tract infections in men. Ann. Intern. Med. 110, 138\u2013150. https:\/\/doi.org\/10.7326\/0003-4819-110-2-138 (1989).","journal-title":"Ann. Intern. Med."},{"key":"21371_CR5","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1086\/315827","volume":"182","author":"D Scholes","year":"2000","unstructured":"Scholes, D. et al. Risk factors for recurrent urinary tract infection in young women. J. Infect. Dis. 182, 1177\u20131182. https:\/\/doi.org\/10.1086\/315827 (2000).","journal-title":"J. Infect. Dis."},{"key":"21371_CR6","doi-asserted-by":"publisher","first-page":"46","DOI":"10.7326\/0003-4819-56-1-46","volume":"56","author":"EH Kass","year":"1962","unstructured":"Kass, E. H. Pyelonephritis and bacteriuria. A major problem in preventive medicine. Ann. Intern. Med. 56, 46\u201353. https:\/\/doi.org\/10.7326\/0003-4819-56-1-46 (1962).","journal-title":"Ann. Intern. Med."},{"key":"21371_CR7","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.1086\/383029","volume":"38","author":"ML Wilson","year":"2004","unstructured":"Wilson, M. L. & Gaido, L. Laboratory diagnosis of urinary tract infections in adult patients. Clin. Infect. Dis. 38, 1150\u20131158. https:\/\/doi.org\/10.1086\/383029 (2004).","journal-title":"Clin. Infect. Dis."},{"key":"21371_CR8","doi-asserted-by":"publisher","first-page":"3183","DOI":"10.2147\/idr.S325770","volume":"14","author":"R El-Mahdy","year":"2021","unstructured":"El-Mahdy, R., Mahmoud, R. & Shrief, R. Characterization of E. coli phylogroups causing catheter-associated urinary tract infection. Infect. Drug Resist. 14, 3183\u20133193. https:\/\/doi.org\/10.2147\/idr.S325770 (2021).","journal-title":"Infect. Drug Resist."},{"key":"21371_CR9","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1046\/j.1469-0691.2000.00057.x","volume":"6","author":"E Draghijeva","year":"2000","unstructured":"Draghijeva, E. Virulence factors in Escherichia coli from children with pyelonephritis. Clin. Microbiol. Infect. 6, 328\u2013330. https:\/\/doi.org\/10.1046\/j.1469-0691.2000.00057.x (2000).","journal-title":"Clin. Microbiol. Infect."},{"key":"21371_CR10","doi-asserted-by":"publisher","first-page":"1365","DOI":"10.1007\/s00467-010-1461-x","volume":"25","author":"S Leroy","year":"2010","unstructured":"Leroy, S. et al. Impressive renal damage after acute pyelonephritis in a child. Pediatr. Nephrol. 25, 1365\u20131368. https:\/\/doi.org\/10.1007\/s00467-010-1461-x (2010).","journal-title":"Pediatr. Nephrol."},{"key":"21371_CR11","doi-asserted-by":"publisher","first-page":"57","DOI":"10.4103\/0974-777X.52984","volume":"1","author":"OP Kalra","year":"2009","unstructured":"Kalra, O. P. & Raizada, A. Approach to a patient with Urosepsis. J. Glob Infect. Dis. 1, 57\u201363. https:\/\/doi.org\/10.4103\/0974-777X.52984 (2009).","journal-title":"J. Glob Infect. Dis."},{"key":"21371_CR12","unstructured":"World, H. & Organization WHO publishes list of bacteria for which new antibiotics are urgently needed, (2017). https:\/\/www.who.int\/news\/item\/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed"},{"key":"21371_CR13","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1016\/j.jiph.2021.11.008","volume":"14","author":"MR Rameshkumar","year":"2021","unstructured":"Rameshkumar, M. R. et al. Occurrence of extended-spectrum \u03b2-lactamase, AmpC, and carbapenemase-producing genes in gram-negative bacterial isolates from human immunodeficiency virus infected patients. J. Infect. Public. Health. 14, 1881\u20131886. https:\/\/doi.org\/10.1016\/j.jiph.2021.11.008 (2021).","journal-title":"J. Infect. Public. Health"},{"key":"21371_CR14","first-page":"11","volume":"17","author":"S Ghafourian","year":"2015","unstructured":"Ghafourian, S., Sadeghifard, N., Soheili, S. & Sekawi, Z. Extended spectrum beta-lactamases: definition, classification and epidemiology. Curr. Issues Mol. Biol. 17, 11\u201322 (2015).","journal-title":"Curr. Issues Mol. Biol."},{"key":"21371_CR15","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/S1473-3099(08)70041-0","volume":"8","author":"JDD Pitout","year":"2008","unstructured":"Pitout, J. D. D. & Laupland, K. B. Extended-spectrum \u03b2-lactamase-producing enterobacteriaceae: an emerging public-health concern. Lancet Infect. Dis. 8, 159\u2013166. https:\/\/doi.org\/10.1016\/S1473-3099(08)70041-0 (2008).","journal-title":"Lancet Infect. Dis."},{"key":"21371_CR16","first-page":"172","volume":"10","author":"JR Zahar","year":"2009","unstructured":"Zahar, J. R. et al. Addressing the challenge of extended-spectrum beta-lactamases. Curr. Opin. Invest. Drugs (London England: 2000). 10, 172\u2013180 (2009).","journal-title":"Curr. Opin. Invest. Drugs (London England: 2000)"},{"key":"21371_CR17","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.1128\/aac.01707-08","volume":"53","author":"A Carattoli","year":"2009","unstructured":"Carattoli, A. Resistance plasmid families in Enterobacteriaceae. Antimicrob. Agents Chemother. 53, 2227\u20132238. https:\/\/doi.org\/10.1128\/aac.01707-08 (2009).","journal-title":"Antimicrob. Agents Chemother."},{"key":"21371_CR18","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.sjbs.2014.08.002","volume":"22","author":"S Shaikh","year":"2015","unstructured":"Shaikh, S., Fatima, J., Shakil, S., Rizvi, S. M. D. & Kamal, M. A. Antibiotic resistance and extended spectrum beta-lactamases: types, epidemiology and treatment. Saudi J. Biol. Sci. 22, 90\u2013101. https:\/\/doi.org\/10.1016\/j.sjbs.2014.08.002 (2015).","journal-title":"Saudi J. Biol. Sci."},{"key":"21371_CR19","first-page":"749","volume":"4","author":"A Shrestha","year":"2016","unstructured":"Shrestha, A., Manandhar, S., Pokharel, P., Panthi, P. & Chaudhary, D. Prevalence of extended spectrum beta-lactamase (ESBL) producing multidrug resistance gram-negative isolates causing urinary tract infection. EC Microbiol. 4, 749\u2013755 (2016).","journal-title":"EC Microbiol."},{"key":"21371_CR20","doi-asserted-by":"publisher","first-page":"1476","DOI":"10.1186\/1476-0711-12-7","volume":"12","author":"SMG Al-Mayahie","year":"2013","unstructured":"Al-Mayahie, S. M. G. Phenotypic and genotypic comparison of ESBL production by vaginal Escherichia coli isolates from pregnant and non-pregnant women. Ann. Clin. Microbiol. Antimicrob. 12, 1476\u20130711. https:\/\/doi.org\/10.1186\/1476-0711-12-7 (2013).","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"21371_CR21","doi-asserted-by":"publisher","first-page":"e01687","DOI":"10.1128\/jcm.01687-23","volume":"62","author":"G Peirano","year":"2024","unstructured":"Peirano, G. et al. Clinical validation of loop-mediated isothermal amplification for the detection of Escherichia coli sequence type complex 131. J. Clin. Microbiol. 62, e01687\u2013e01623. https:\/\/doi.org\/10.1128\/jcm.01687-23 (2024).","journal-title":"J. Clin. Microbiol."},{"key":"21371_CR22","doi-asserted-by":"publisher","unstructured":"Adekanmbi, A. O., Oluwaseyi, T. A. & Oyelade, A. A. Dumpsite leachate as a hotspot of multidrug resistant Enterobacteriaceae harbouring extended spectrum and AmpC \u03b2-lactamase genes; a case study of Awotan municipal solid waste dumpsite in Southwest Nigeria. Meta Gene. 28 https:\/\/doi.org\/10.1016\/j.mgene.2021.100853 (2021).","DOI":"10.1016\/j.mgene.2021.100853"},{"key":"21371_CR23","doi-asserted-by":"crossref","unstructured":"Silago, V. et al. Existence of multiple ESBL genes among phenotypically confirmed ESBL producing Klebsiella pneumoniae and Escherichia coli concurrently isolated from clinical, colonization and contamination samples from neonatal units at Bugando Medical Center, Mwanza, Tanzania. Antibiotics 10, 476 (2021).","DOI":"10.3390\/antibiotics10050476"},{"key":"21371_CR24","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1093\/cid\/ciw283","volume":"63","author":"S Karanika","year":"2016","unstructured":"Karanika, S., Karantanos, T., Arvanitis, M., Grigoras, C. & Mylonakis, E. Fecal colonization with extended-spectrum beta-lactamase\u2013producing Enterobacteriaceae and risk factors among healthy individuals: a systematic review and metaanalysis. Clin. Infect. Dis. 63, 310\u2013318. https:\/\/doi.org\/10.1093\/cid\/ciw283 (2016).","journal-title":"Clin. Infect. Dis."},{"key":"21371_CR25","doi-asserted-by":"publisher","first-page":"122","DOI":"10.3201\/eid0802.010141","volume":"8","author":"P Houpikian","year":"2002","unstructured":"Houpikian, P. & Raoult, D. Traditional and molecular techniques for the study of emerging bacterial diseases: one laboratory\u2019s perspective. Emerg. Infect. Dis. 8, 122. https:\/\/doi.org\/10.3201\/eid0802.010141 (2002).","journal-title":"Emerg. Infect. Dis."},{"key":"21371_CR26","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1128\/jcm.38.2.635-638.2000","volume":"38","author":"E Aguero-Rosenfeld Maria","year":"2000","unstructured":"Aguero-Rosenfeld Maria, E. et al. Serology of culture-confirmed cases of human granulocytic ehrlichiosis. J. Clin. Microbiol. 38, 635\u2013638. https:\/\/doi.org\/10.1128\/jcm.38.2.635-638.2000 (2000).","journal-title":"J. Clin. Microbiol."},{"key":"21371_CR27","doi-asserted-by":"publisher","unstructured":"Alanazi, M., Alqahtani, F. & Aleanizy, F. An evaluation of E. coli in urinary tract infection in emergency department at KAMC in Riyadh, Saudi arabia: retrospective study. Ann. Clin. Microbiol. Antimicrob. 17 https:\/\/doi.org\/10.1186\/s12941-018-0255-z (2018).","DOI":"10.1186\/s12941-018-0255-z"},{"key":"21371_CR28","doi-asserted-by":"publisher","first-page":"72","DOI":"10.3923\/ajbmb.2016.72.83","volume":"6","author":"A Elsayed","year":"2016","unstructured":"Elsayed, A., Mohamedin, A., Ata, T. & Ghazala, N. Molecular characterization of multidrug resistant clinical Escherichia coli isolates. Am. J. Biochem. Mol. Biol. 6, 72\u201383. https:\/\/doi.org\/10.3923\/ajbmb.2016.72.83 (2016).","journal-title":"Am. J. Biochem. Mol. Biol."},{"key":"21371_CR29","doi-asserted-by":"publisher","unstructured":"Liu, Y. et al. Detection of 12 common food-borne bacterial pathogens by TaqMan real-time PCR using a single set of reaction conditions. Front. Microbiol. 10 https:\/\/doi.org\/10.3389\/fmicb.2019.00222 (2019).","DOI":"10.3389\/fmicb.2019.00222"},{"key":"21371_CR30","doi-asserted-by":"publisher","first-page":"14558","DOI":"10.1038\/s41598-022-18765-w","volume":"12","author":"T Ruang-areerate","year":"2022","unstructured":"Ruang-areerate, T. et al. Distance-based paper device using combined SYBR safe and gold nanoparticle probe LAMP assay to detect Leishmania among patients with HIV. Sci. Rep. 12, 14558. https:\/\/doi.org\/10.1038\/s41598-022-18765-w (2022).","journal-title":"Sci. Rep."},{"key":"21371_CR31","doi-asserted-by":"publisher","first-page":"e63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, e63\u2013e63. https:\/\/doi.org\/10.1093\/nar\/28.12.e63 (2000).","journal-title":"Nucleic Acids Res."},{"key":"21371_CR32","doi-asserted-by":"crossref","unstructured":"Itou, T., Markotter, W. & Nel, L. H. in Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention Vol. 1 (eds Charles Rupprecht & Thirumeni Nagarajan) 85\u201395Academic Press, (2014).","DOI":"10.1016\/B978-0-12-800014-4.00008-1"},{"key":"21371_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s12275-015-4656-9","volume":"53","author":"T Notomi","year":"2015","unstructured":"Notomi, T., Mori, Y., Tomita, N. & Kanda, H. Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J. Microbiol. 53, 1\u20135. https:\/\/doi.org\/10.1007\/s12275-015-4656-9 (2015).","journal-title":"J. Microbiol."},{"key":"21371_CR34","doi-asserted-by":"publisher","first-page":"114178","DOI":"10.1016\/j.jpba.2021.114178","volume":"203","author":"P Suwannin","year":"2021","unstructured":"Suwannin, P. et al. Heat-enhancing aggregation of gold nanoparticles combined with loop-mediated isothermal amplification (HAG-LAMP) for Plasmodium falciparum detection. J. Pharm. Biomed. Anal. 203, 114178. https:\/\/doi.org\/10.1016\/j.jpba.2021.114178 (2021).","journal-title":"J. Pharm. Biomed. Anal."},{"key":"21371_CR35","doi-asserted-by":"publisher","first-page":"12152","DOI":"10.1038\/s41598-021-91540-5","volume":"11","author":"T Ruang-areerate","year":"2021","unstructured":"Ruang-areerate, T. et al. Development of loop-mediated isothermal amplification (LAMP) assay using SYBR safe and gold-nanoparticle probe for detection of Leishmania in HIV patients. Sci. Rep. 11, 12152. https:\/\/doi.org\/10.1038\/s41598-021-91540-5 (2021).","journal-title":"Sci. Rep."},{"key":"21371_CR36","doi-asserted-by":"publisher","unstructured":"Sharif, S. et al. A novel impedimetric sensor for detecting LAMP amplicons of pathogenic DNA based on magnetic separation. Sens. Actuators B: Chem. 301 https:\/\/doi.org\/10.1016\/j.snb.2019.127051 (2019).","DOI":"10.1016\/j.snb.2019.127051"},{"key":"21371_CR37","doi-asserted-by":"publisher","unstructured":"Zhang, M. et al. A newly developed paper embedded microchip based on LAMP for rapid multiple detections of foodborne pathogens. BMC Microbiol. 21 https:\/\/doi.org\/10.1186\/s12866-021-02223-0 (2021).","DOI":"10.1186\/s12866-021-02223-0"},{"key":"21371_CR38","doi-asserted-by":"publisher","first-page":"8077","DOI":"10.1039\/D0AN01306D","volume":"145","author":"N Saengsawang","year":"2020","unstructured":"Saengsawang, N. et al. Development of a fluorescent distance-based paper device using loop-mediated isothermal amplification to detect Escherichia coli in urine. Analyst 145, 8077\u20138086. https:\/\/doi.org\/10.1039\/D0AN01306D (2020).","journal-title":"Analyst"},{"key":"21371_CR39","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.talanta.2017.07.019","volume":"175","author":"I Hongwarittorrn","year":"2017","unstructured":"Hongwarittorrn, I., Chaichanawongsaroj, N. & Laiwattanapaisal, W. Semi-quantitative visual detection of loop mediated isothermal amplification (LAMP)-generated DNA by distance-based measurement on a paper device. Talanta 175, 135\u2013142. https:\/\/doi.org\/10.1016\/j.talanta.2017.07.019 (2017).","journal-title":"Talanta"},{"key":"21371_CR40","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.jgar.2017.01.011","volume":"9","author":"A Thuengern","year":"2017","unstructured":"Thuengern, A., Nathisuwan, S., Chomnawang, M. T., Wiwat, C. & Thirapanmethee, K. Genotypic detection of the blaCTX-M-1 gene among extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae. J. Glob Antimicrob. Resist. 9, 87\u201393. https:\/\/doi.org\/10.1016\/j.jgar.2017.01.011 (2017).","journal-title":"J. Glob Antimicrob. Resist."},{"key":"21371_CR41","doi-asserted-by":"publisher","first-page":"eaal3693","DOI":"10.1126\/scitranslmed.aal3693","volume":"9","author":"NG Schoepp","year":"2017","unstructured":"Schoepp, N. G. et al. Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples. Sci. Transl Med. 9, eaal3693. https:\/\/doi.org\/10.1126\/scitranslmed.aal3693 (2017).","journal-title":"Sci. Transl Med."},{"key":"21371_CR42","doi-asserted-by":"publisher","first-page":"16426","DOI":"10.1021\/acs.analchem.3c04316","volume":"95","author":"W Wu","year":"2023","unstructured":"Wu, W. et al. Enzymatic antimicrobial susceptibility testing with bacteria identification in 30 min. Anal. Chem. 95, 16426\u201316432. https:\/\/doi.org\/10.1021\/acs.analchem.3c04316 (2023).","journal-title":"Anal. Chem."},{"key":"21371_CR43","doi-asserted-by":"publisher","first-page":"17853","DOI":"10.1021\/acs.analchem.2c03604","volume":"94","author":"W Wu","year":"2022","unstructured":"Wu, W. et al. All-In-One Escherichia coli viability assay for multi-dimensional detection of uncomplicated urinary tract infections. Anal. Chem. 94, 17853\u201317860. https:\/\/doi.org\/10.1021\/acs.analchem.2c03604 (2022).","journal-title":"Anal. Chem."},{"key":"21371_CR44","doi-asserted-by":"publisher","first-page":"2399","DOI":"10.1039\/D2LC01095J","volume":"23","author":"W Wu","year":"2023","unstructured":"Wu, W. et al. Direct single-cell antimicrobial susceptibility testing of Escherichia coli in urine using a ready-to-use 3D microwell array chip. Lab. Chip. 23, 2399\u20132410. https:\/\/doi.org\/10.1039\/D2LC01095J (2023).","journal-title":"Lab. Chip"},{"key":"21371_CR45","first-page":"495","volume":"36","author":"T Thita","year":"2019","unstructured":"Thita, T., Manomat, J., Leelayoova, S., Mungthin, M. & Ruang-areerate, T. Reliable interpretation and long-term stability using SYBR\u2122 safe fluorescent assay for loop-mediated isothermal amplification (LAMP) detection of Leishmania spp. Trop. Biomed. 36, 495\u2013504 (2019).","journal-title":"Trop. Biomed."},{"key":"21371_CR46","doi-asserted-by":"publisher","first-page":"18781","DOI":"10.1038\/s41598-023-46001-6","volume":"13","author":"N Saengsawang","year":"2023","unstructured":"Saengsawang, N. et al. Validation of quantitative loop-mediated isothermal amplification assay using a fluorescent distance-based paper device for detection of Escherichia coli in urine. Sci. Rep. 13, 18781. https:\/\/doi.org\/10.1038\/s41598-023-46001-6 (2023).","journal-title":"Sci. Rep."},{"key":"21371_CR47","doi-asserted-by":"publisher","first-page":"2151","DOI":"10.2147\/IDR.S174506","volume":"11","author":"J Bubpamala","year":"2018","unstructured":"Bubpamala, J. et al. Phenotypic and genotypic characterizations of extended-spectrum beta-lactamase-producing Escherichia coli in Thailand. Infect. Drug Resist. 11, 2151\u20132157. https:\/\/doi.org\/10.2147\/IDR.S174506 (2018).","journal-title":"Infect. Drug Resist."},{"key":"21371_CR48","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1093\/jac\/dkad031","volume":"78","author":"A Moreno-Mingorance","year":"2023","unstructured":"Moreno-Mingorance, A. et al. Increasing trend of antimicrobial resistance in Shigella associated with MSM transmission in Barcelona, 2020\u201321: outbreak of XRD Shigella sonnei and dissemination of ESBL-producing Shigella flexneri. J. Antimicrob. Chemother. 78, 975\u2013982. https:\/\/doi.org\/10.1093\/jac\/dkad031 (2023).","journal-title":"J. Antimicrob. Chemother."},{"key":"21371_CR49","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1089\/mdr.2019.0235","volume":"26","author":"K Shahin","year":"2020","unstructured":"Shahin, K., Bouzari, M., Komijani, M. & Wang, R. A new phage cocktail against multidrug, ESBL-producer isolates of Shigella sonnei and Shigella flexneri with highly efficient bacteriolytic activity. Microb. Drug Resist. 26, 831\u2013841. https:\/\/doi.org\/10.1089\/mdr.2019.0235 (2020).","journal-title":"Microb. Drug Resist."},{"key":"21371_CR50","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1016\/j.cmi.2017.04.004","volume":"23","author":"S Heytens","year":"2017","unstructured":"Heytens, S. et al. Women with symptoms of a urinary tract infection but a negative urine culture: PCR-based quantification of Escherichia coli suggests infection in most cases. Clin. Microbiol. Infect. 23, 647\u2013652. https:\/\/doi.org\/10.1016\/j.cmi.2017.04.004 (2017).","journal-title":"Clin. Microbiol. Infect."},{"key":"21371_CR51","doi-asserted-by":"publisher","first-page":"2800","DOI":"10.1128\/jcm.00152-08","volume":"46","author":"J Hill","year":"2008","unstructured":"Hill, J. et al. Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli. J. Clin. Microbiol. 46, 2800\u20132804. https:\/\/doi.org\/10.1128\/jcm.00152-08 (2008).","journal-title":"J. Clin. Microbiol."},{"key":"21371_CR52","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1111\/j.1574-695X.2010.00676.x","volume":"59","author":"B Hu","year":"2010","unstructured":"Hu, B. et al. Structural and genetic evidence for the close relationship between Escherichia coli O71 and Salmonella enterica O28 O-antigens. FEMS Immunol. Med. Microbiol. 59, 161\u2013169. https:\/\/doi.org\/10.1111\/j.1574-695X.2010.00676.x (2010).","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"21371_CR53","unstructured":"CLSI. Vol. 34 (ed MA Megan L. Tertel). Clinical and Laboratory Standards Institute, Wayne, PA, (2014)."},{"key":"21371_CR54","doi-asserted-by":"publisher","first-page":"8682","DOI":"10.1038\/s41598-018-26982-5","volume":"8","author":"D Yuan","year":"2018","unstructured":"Yuan, D., Kong, J., Li, X., Fang, X. & Chen, Q. Colorimetric LAMP microfluidic chip for detecting three allergens: peanut, Sesame and soybean. Sci. Rep. 8, 8682. https:\/\/doi.org\/10.1038\/s41598-018-26982-5 (2018).","journal-title":"Sci. Rep."},{"key":"21371_CR55","doi-asserted-by":"publisher","first-page":"5548","DOI":"10.1038\/s41598-018-23930-1","volume":"8","author":"VJ Gadkar","year":"2018","unstructured":"Gadkar, V. J., Goldfarb, D. M., Gantt, S. & Tilley, P. A. G. Real-time detection and monitoring of loop mediated amplification (LAMP) reaction using self-quenching and de-quenching fluorogenic probes. Sci. Rep. 8, 5548. https:\/\/doi.org\/10.1038\/s41598-018-23930-1 (2018).","journal-title":"Sci. Rep."},{"key":"21371_CR56","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1186\/s13071-015-1202-x","volume":"8","author":"C Sriworarat","year":"2015","unstructured":"Sriworarat, C., Phumee, A., Mungthin, M., Leelayoova, S. & Siriyasatien, P. Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection. Parasit. Vectors. 8, 591. https:\/\/doi.org\/10.1186\/s13071-015-1202-x (2015).","journal-title":"Parasit. Vectors"},{"key":"21371_CR57","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1038\/s41598-024-83214-9","volume":"15","author":"A Suwanngam","year":"2025","unstructured":"Suwanngam, A. et al. Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues. Sci. Rep. 15, 160. https:\/\/doi.org\/10.1038\/s41598-024-83214-9 (2025).","journal-title":"Sci. Rep."},{"key":"21371_CR58","doi-asserted-by":"publisher","first-page":"2818","DOI":"10.1128\/aac.00171-08","volume":"52","author":"P Kiratisin","year":"2008","unstructured":"Kiratisin, P., Apisarnthanarak, A., Laesripa, C. & Saifon, P. Molecular characterization and epidemiology of extended-spectrum- \u03b2-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infection in Thailand, where the CTX-M family is endemic. Antimicrob. Agents Chemother. 52, 2818\u20132824. https:\/\/doi.org\/10.1128\/aac.00171-08 (2008).","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21371-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21371-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21371-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T02:03:34Z","timestamp":1761962614000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21371-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,27]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["21371"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-21371-1","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,27]]},"assertion":[{"value":"6 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 September 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 October 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"37429"}}