{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T15:34:53Z","timestamp":1761752093867,"version":"build-2065373602"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100005031","name":"Bhabha Atomic Research Centre","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005031","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-21739-3","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T15:31:58Z","timestamp":1761751918000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cardio-renal protection and mechanisms of the antihypertensive activity of Alcalase-derived moth bean hydrolysate in DOCA-salt induced hypertensive Wistar rats"],"prefix":"10.1038","volume":"15","author":[{"given":"Nancy","family":"Goyal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitesh Singh","family":"Rathee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachin N.","family":"Hajare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Gautam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"21739_CR1","doi-asserted-by":"publisher","first-page":"3021","DOI":"10.1093\/eurheartj\/ehy339","volume":"39","author":"B Williams","year":"2018","unstructured":"Williams, B. et al. 2018 ESC\/ESH guidelines for the management of arterial hypertension. Eur. Heart J. 39, 3021\u20133104 (2018).","journal-title":"Eur. Heart J."},{"key":"21739_CR2","first-page":"1153","volume":"9","author":"A Kaleem","year":"2020","unstructured":"Kaleem, A., Ahmed, S. & Hassan, M. M. Vigna aconitifolia (Jacq.) Marechal. (Papilionaceae): A review of medicinal uses, phytochemistry and Pharmacology. J. Pharmacognosy Phytochem. 9, 1153\u20131155 (2020).","journal-title":"J. Pharmacognosy Phytochemistry"},{"key":"21739_CR3","doi-asserted-by":"publisher","first-page":"101088","DOI":"10.1016\/j.fbio.2021.101088","volume":"42","author":"A Bhadkaria","year":"2021","unstructured":"Bhadkaria, A., Srivastava, N. & Bhagyawant, S. S. A prospective of underutilized legume moth bean (Vigna aconitifolia (Jacq.) Marech\u00e0l): phytochemical profiling, bioactive compounds and in vitro\u00a0pharmacological studies. Food Bioscience. 42, 101088 (2021).","journal-title":"Food Bioscience"},{"key":"21739_CR4","doi-asserted-by":"publisher","first-page":"1167259","DOI":"10.3389\/fnut.2023.1167259","volume":"10","author":"N Goyal","year":"2023","unstructured":"Goyal, N., Hajare, S. N. & Gautam, S. Release of an encrypted, highly potent ACE-inhibitory peptide by enzymatic hydrolysis of moth bean (Vigna aconitifolia) protein. Front. Nutr. 10, 1167259 (2023).","journal-title":"Front. Nutr."},{"key":"21739_CR5","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1021\/acsfoodscitech.4c00784","volume":"5","author":"N Goyal","year":"2025","unstructured":"Goyal, N., Adiani, V., Hajare, S. N. & Gautam, S. Characterization of phenolic compounds from moth bean (Vigna aconitifolia) seeds and assessment of prophylactic potential in terms of antioxidant and antihypertensive activity. ACS Food Sci. Technol. 5, 359\u2013367 (2025).","journal-title":"ACS Food Sci. Technol."},{"key":"21739_CR6","doi-asserted-by":"publisher","first-page":"111","DOI":"10.3109\/10715762.2015.1112005","volume":"50","author":"JS Rathee","year":"2016","unstructured":"Rathee, J. S., Patro, B. S., Brown, L. & Chattopadhyay, S. Mechanism of the anti-hypertensive property of the naturally occurring phenolic, Malabaricone C in DOCA-salt rats. Free Radic. Res. 50, 111\u2013121 (2016).","journal-title":"Free Radic. Res."},{"key":"21739_CR7","doi-asserted-by":"publisher","first-page":"651246","DOI":"10.3389\/fphys.2021.651246","volume":"12","author":"CS Cerrudo","year":"2021","unstructured":"Cerrudo, C. S. et al. Cardiac natriuretic peptide profiles in chronic hypertension by single or sequentially combined renovascular and DOCA-salt treatments. Front. Physiol. 12, 651246 (2021).","journal-title":"Front. Physiol."},{"key":"21739_CR8","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1146\/annurev-food-022814-015520","volume":"6","author":"RE Aluko","year":"2015","unstructured":"Aluko, R. E. Antihypertensive peptides from food proteins. Annu. Rev. Food Sci. Technol. 6, 235\u2013262 (2015).","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"21739_CR9","first-page":"1","volume":"27","author":"O Erbas","year":"2012","unstructured":"Erbas, O. P-1081 - The effects of Metoprolol and diltiazem in prolonged QTc interval caused by Ziprasidone injection in rats. Eur. Psychiatry. 27, 1 (2012).","journal-title":"Eur. Psychiatry"},{"key":"21739_CR10","doi-asserted-by":"publisher","first-page":"2556","DOI":"10.1038\/s41598-023-28325-5","volume":"13","author":"CW Liu","year":"2023","unstructured":"Liu, C. W. et al. Left ventricular hypertrophy detection using electrocardiographic signal. Sci. Rep. 13, 2556 (2023).","journal-title":"Sci. Rep."},{"key":"21739_CR11","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1007\/s00467-021-05144-2","volume":"37","author":"S Genovesi","year":"2022","unstructured":"Genovesi, S. et al. Relationship between endothelin and nitric oxide pathways in the onset and maintenance of hypertension in children and adolescents. Pediatr. Nephrol. 37, 537\u2013545 (2022).","journal-title":"Pediatr. Nephrol."},{"key":"21739_CR12","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1161\/01.HYP.30.1.29","volume":"30","author":"S Rajagopalan","year":"1997","unstructured":"Rajagopalan, S. et al. Role for Endothelin-1 in angiotensin II\u2013 mediated hypertension. Hypertension 30, 29\u201334 (1997).","journal-title":"Hypertension"},{"key":"21739_CR13","doi-asserted-by":"publisher","first-page":"2322","DOI":"10.2337\/dc18-1307","volume":"41","author":"P Muntner","year":"2018","unstructured":"Muntner, P., Whelton, P. K., Woodward, M. & Carey, R. M. A comparison of the 2017 American college of Cardiology\/American heart association blood pressure guideline and the 2017 American diabetes association diabetes and hypertension position statement for US adults with diabetes. Diabetes Care. 41, 2322\u20132329 (2018).","journal-title":"Diabetes Care"},{"key":"21739_CR14","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.tifs.2017.07.011","volume":"69","author":"J Wu","year":"2017","unstructured":"Wu, J., Liao, W. & Udenigwe, C. C. Revisiting the mechanisms of ACE inhibitory peptides from food proteins. Trends Food Sci. Technol. 69, 214\u2013219 (2017).","journal-title":"Trends Food Sci. Technol."},{"key":"21739_CR15","doi-asserted-by":"publisher","first-page":"H393","DOI":"10.1152\/ajpheart.00075.2012","volume":"303","author":"PS Guimaraes","year":"2012","unstructured":"Guimaraes, P. S. et al. Chronic infusion of angiotensin-(1\u20137) into the lateral ventricle of the brain attenuates hypertension in DOCA-salt rats. Am. J. Physiol. Heart Circ. Physiol. 303, H393\u2013H400 (2012).","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"21739_CR16","first-page":"27","volume":"4","author":"KA Connelly","year":"2019","unstructured":"Connelly, K. A. et al. Empagliflozin improves diastolic function in a nondiabetic rodent model of heart failure with preserved ejection fraction. JACC: Basic. Translational Sci. 4, 27\u201337 (2019).","journal-title":"JACC: Basic. Translational Sci."},{"key":"21739_CR17","doi-asserted-by":"publisher","first-page":"18623","DOI":"10.1038\/s41598-020-75607-3","volume":"10","author":"CR Nwokocha","year":"2020","unstructured":"Nwokocha, C. R. et al. Hypotensive and antihypertensive effects of an aqueous extract from guinep fruit (Melicoccus bijugatus Jacq) in rats. Sci. Rep. 10, 18623 (2020).","journal-title":"Sci. Rep."},{"key":"21739_CR18","doi-asserted-by":"publisher","DOI":"10.36660\/ijcs.20190116","author":"AG Concei\u00e7\u00e3o-Vertamatti","year":"2019","unstructured":"Concei\u00e7\u00e3o-Vertamatti, A. G. et al. Electrocardiographic abnormalities in hypertension models: ECG in hypertension models. IJCS https:\/\/doi.org\/10.36660\/ijcs.20190116 (2019).","journal-title":"IJCS"},{"key":"21739_CR19","doi-asserted-by":"crossref","unstructured":"Cirillo, R. et al. Comparison of Doxorubicin- and MEN 10755-induced long-term progressive cardiotoxicity in the rat. J. Cardiovasc. Pharmacol. 35, (2000).","DOI":"10.1097\/00005344-200001000-00013"},{"key":"21739_CR20","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1002\/ptr.1739","volume":"19","author":"I Obiefuna","year":"2005","unstructured":"Obiefuna, I. & Young, R. Concurrent administration of aqueous Azadirachta indica (neem) leaf extract with DOCA-salt prevents the development of hypertension and accompanying electrocardiogram changes in the rat. Phytother Res. 19, 792\u2013795 (2005).","journal-title":"Phytother Res."},{"key":"21739_CR21","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1093\/eurheartj\/ehr304","volume":"33","author":"U F\u00f6rstermann","year":"2012","unstructured":"F\u00f6rstermann, U. & Sessa, W. C. Nitric oxide synthases: regulation and function. Eur. Heart J. 33, 829\u2013837 (2012).","journal-title":"Eur. Heart J."},{"key":"21739_CR22","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1038\/s41569-019-0260-8","volume":"17","author":"Y Zhang","year":"2020","unstructured":"Zhang, Y., Murugesan, P., Huang, K. & Cai, H. NADPH oxidases and oxidase crosstalk in cardiovascular diseases: novel therapeutic targets. Nat. Rev. Cardiol. 17, 170\u2013194 (2020).","journal-title":"Nat. Rev. Cardiol."},{"key":"21739_CR23","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1161\/CIRCRESAHA.121.318063","volume":"128","author":"KK Griendling","year":"2021","unstructured":"Griendling, K. K. et al. Oxidative stress and hypertension. Circul. Res. 128, 993\u20131020 (2021).","journal-title":"Circul. Res."},{"key":"21739_CR24","doi-asserted-by":"publisher","first-page":"3579","DOI":"10.2174\/13816128113196660748","volume":"20","author":"S Karbach","year":"2014","unstructured":"Karbach, S., Wenzel, P., Waisman, A., Munzel, T. & Daiber, A. eNOS uncoupling in cardiovascular diseases - the role of oxidative stress and inflammation. CPD 20, 3579\u20133594 (2014).","journal-title":"CPD"},{"key":"21739_CR25","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s11906-012-0320-5","volume":"15","author":"R Thoonen","year":"2013","unstructured":"Thoonen, R., Sips, P. Y., Bloch, K. D. & Buys, E. S. Pathophysiology of hypertension in the absence of nitric oxide\/Cyclic GMP signaling. Curr. Hypertens. Rep. 15, 47\u201358 (2013).","journal-title":"Curr. Hypertens. Rep."},{"key":"21739_CR26","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1016\/j.jep.2016.10.008","volume":"193","author":"AA Syed","year":"2016","unstructured":"Syed, A. A. et al. Evaluation of anti-hypertensive activity of Ulmus Wallichiana extract and fraction in SHR, DOCA-salt- and L-NAME-induced hypertensive rats. J. Ethnopharmacol. 193, 555\u2013565 (2016).","journal-title":"J. Ethnopharmacol."},{"key":"21739_CR27","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1016\/j.metabol.2011.12.012","volume":"61","author":"P Prahalathan","year":"2012","unstructured":"Prahalathan, P., Kumar, S. & Raja, B. Morin attenuates blood pressure and oxidative stress in deoxycorticosterone acetate-salt hypertensive rats: A biochemical and histopathological evaluation. Metabolism 61, 1087\u20131099 (2012).","journal-title":"Metabolism"},{"key":"21739_CR28","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1111\/anec.12223","volume":"19","author":"L Bacharova","year":"2014","unstructured":"Bacharova, L. & Ugander, M. Left ventricular hypertrophy: the relationship between the electrocardiogram and cardiovascular magnetic resonance imaging. Noninvasive Electrocardiol. 19, 524\u2013533 (2014).","journal-title":"Noninvasive Electrocardiol."},{"key":"21739_CR29","doi-asserted-by":"publisher","unstructured":"Ogawa, M. et al. Evaluation of the association between electrocardiogram parameters and left cardiac remodeling in dogs with myxomatous mitral valve disease. Vet. World. 2072\u20132083. https:\/\/doi.org\/10.14202\/vetworld.2022.2072-2083 (2022).","DOI":"10.14202\/vetworld.2022.2072-2083"},{"key":"21739_CR30","doi-asserted-by":"publisher","first-page":"291","DOI":"10.2174\/157340310793566109","volume":"6","author":"A Iyer","year":"2010","unstructured":"Iyer, A., Chan, V. & Brown, L. The DOCA-Salt hypertensive rat as a model of cardiovascular oxidative and inflammatory stress. CCR 6, 291\u2013297 (2010).","journal-title":"CCR"},{"key":"21739_CR31","doi-asserted-by":"publisher","first-page":"2204","DOI":"10.1016\/j.jacc.2020.03.019","volume":"75","author":"J D\u00edez","year":"2020","unstructured":"D\u00edez, J., Gonz\u00e1lez, A. & Kovacic, J. C. Myocardial interstitial fibrosis in nonischemic heart disease, part 3\/4. J. Am. Coll. Cardiol. 75, 2204\u20132218 (2020).","journal-title":"J. Am. Coll. Cardiol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21739-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21739-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21739-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T15:32:02Z","timestamp":1761751922000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-21739-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["21739"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-21739-3","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]},"assertion":[{"value":"6 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 September 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 October 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The animal study was approved by the BARC Animal Ethics Committee (BAEC\/33\/21 dt. 01\/12\/2021).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}],"article-number":"37821"}}