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This study establishes a spectrofluorimetric method for quantifying CYP in ophthalmic solutions based on Resonance Rayleigh Scattering (RRS) signal modulation. The approach exploits the formation of an ion-associate complex between CYP and the dye erythrosine, which induces a measurable RRS enhancement. Critical experimental parameters governing the complexation and subsequent RRS response were systematically investigated and optimized. Under these established optimal conditions, a linear correlation existed between the enhanced RRS intensity and CYP concentration across the range of 40 to 1500\u00a0ng\/mL. The method demonstrated a detection limit (LOD) of 13\u00a0ng\/mL and a quantification limit (LOQ) of 39.5\u00a0ng\/mL. Successful application to the assay of cyclopentolate in commercial eye drop formulations confirmed the method\u2019s accuracy and precision. This RRS-based methodology utilizing erythrosine presents a straightforward, rapid, cost-effective, and environmentally favorable option for routine quality control and monitoring of CYP in pharmaceutical preparations.<\/jats:p>","DOI":"10.1038\/s41598-025-21762-4","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T09:42:27Z","timestamp":1761558147000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Spectroscopic quantification of cyclopentolate using an erythrosine-based resonance rayleigh scattering strategy: application to ophthalmic formulations"],"prefix":"10.1038","volume":"15","author":[{"given":"Ahmed A.","family":"Abu-hassan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"21762_CR1","doi-asserted-by":"publisher","first-page":"3753","DOI":"10.2147\/OPTH.S388982","volume":"16","author":"H Contreras-Salinas","year":"2022","unstructured":"Contreras-Salinas, H. et al. Ocular cyclopentolate: A mini review concerning its benefits and risks. Clin. Ophthalmol. 16, 3753 (2022).","journal-title":"Clin. Ophthalmol."},{"key":"21762_CR2","first-page":"223","volume":"5","author":"D Ihekaire","year":"2012","unstructured":"Ihekaire, D. The comparative efficacy of cyclopegic drugs\u2013tropicamide and cyclopentolate on school children. Int. J. Sci. Res. Educ. 5, 223\u2013246 (2012).","journal-title":"Int. J. Sci. Res. Educ."},{"key":"21762_CR3","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.optom.2017.09.001","volume":"11","author":"N Yazdani","year":"2018","unstructured":"Yazdani, N., Sadeghi, R., Momeni-Moghaddam, H., Zarifmahmoudi, L. & Ehsaei, A. Comparison of cyclopentolate versus tropicamide cycloplegia: A systematic review and meta-analysis. J. Optom. 11, 135\u2013143 (2018).","journal-title":"J. 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