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A 28-day trial was conducted using 120 weaned piglets that were randomly allocated to two treatment groups with different iron sources. The Fe-sulfate group received a diet containing 100\u00a0mg Fe\/kg in the form of ferrous sulfate, while the Fe-HMA group received a diet with 50\u00a0mg Fe\/kg in the form of Fe-HMA. Fe-HMA, as an iron source, did not significantly affect growth performance, but improved iron apparent digestibility and tissue deposition compared with the Fe-sulfate group. Additionally, Fe-HMA significantly enhanced erythrocyte-related indicators and regulated the serum iron metabolism markers. It also upregulated the relative expression of the iron metabolism-related genes ferroportin1 (\n                    <jats:italic>FPN1<\/jats:italic>\n                    ) in the duodenum and hepcidin antimicrobial peptide (\n                    <jats:italic>HAMP<\/jats:italic>\n                    ), ferritin light chain (\n                    <jats:italic>FTL<\/jats:italic>\n                    ), and transferrin (\n                    <jats:italic>TF<\/jats:italic>\n                    ) in the liver, while downregulating transferrin receptor (\n                    <jats:italic>TFRC<\/jats:italic>\n                    ) expression in the liver. Replacing dietary inorganic iron with HMA-chelated iron improved iron metabolism in weaned piglets, suggesting its potential as an effective alternative source of iron in porcine nutrition.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-22609-8","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T13:25:26Z","timestamp":1762435526000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of hydroxy methionine analog iron chelate on growth performance, blood parameters, and iron metabolism in weaned piglets"],"prefix":"10.1038","volume":"15","author":[{"given":"Yuemeng","family":"Fu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingzi","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilin","family":"Ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuning","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guohui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuejun","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiren","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"22609_CR1","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/S0140-6736(07)61235-5","volume":"370","author":"MB Zimmermann","year":"2007","unstructured":"Zimmermann, M. 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