{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T05:06:36Z","timestamp":1782968796980,"version":"3.54.5"},"reference-count":70,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Financial Code 001"],"award-info":[{"award-number":["Financial Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Financial Code 001"],"award-info":[{"award-number":["Financial Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Financial Code 001"],"award-info":[{"award-number":["Financial Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/02510-5"],"award-info":[{"award-number":["2022\/02510-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018 \/ 06008-0"],"award-info":[{"award-number":["2018 \/ 06008-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["425694 \/ 2016-0"],"award-info":[{"award-number":["425694 \/ 2016-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303035\/2018-8"],"award-info":[{"award-number":["303035\/2018-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-23507-9","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T22:48:28Z","timestamp":1763074108000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mixed granulocytic airway inflammation impairs aversive learning and alters neuroimmune biomarkers in mice"],"prefix":"10.1038","volume":"15","author":[{"given":"Pamela Maria Araujo","family":"Morera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Ivo Ara\u00fajo","family":"Beserra-Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Soares-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Herling","family":"Lambertucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitoria Caroline","family":"de Queiroz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalia Montouro","family":"Pinheiro-Menegasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Cossi","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitor","family":"Ponci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Aparecida","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wothan","family":"Tavares-de-Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Henrique Ghilardi","family":"Lago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra Mussi","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla Maximo","family":"Prado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"23507_CR1","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1183\/09031936.03.00261903","volume":"22","author":"B Abraham","year":"2003","unstructured":"Abraham, B. et al. The ENFUMOSA cross-sectional European multicentre study of the clinical phenotype of chronic severe asthma. Eur. Respir. J. 22, 470\u2013477 (2003).","journal-title":"Eur. Respir. J."},{"key":"23507_CR2","doi-asserted-by":"crossref","unstructured":"Moore, W. C. et al. Sputum neutrophil counts are associated with more severe asthma phenotypes using cluster analysis. J.  Allergy Clin. Immunol. 133, (2014).","DOI":"10.1016\/j.jaci.2013.10.011"},{"key":"23507_CR3","unstructured":"GINA-Global Strategy for Asthma Management and Prevention. GINA-Global Strategy for Asthma Management and Prevention. www.ginaasthma.org  (2024)."},{"key":"23507_CR4","doi-asserted-by":"publisher","first-page":"256","DOI":"10.2500\/aap.2015.36.3847","volume":"36","author":"M Frieri","year":"2015","unstructured":"Frieri, M., O\u2019Connor, M. & Nassef, M. Asthma, stress, and depression in women. Allergy Asthma Proc. 36, 256\u2013261 (2015).","journal-title":"Allergy Asthma Proc."},{"key":"23507_CR5","doi-asserted-by":"publisher","first-page":"398","DOI":"10.3109\/02770903.2015.1126846","volume":"53","author":"F Sundbom","year":"2016","unstructured":"Sundbom, F., Malinovschi, A., Lindberg, E., Alving, K. & Janson, C. Effects of poor asthma control, insomnia, anxiety and deprebion on quality of life in young asthmatics. J. Asthma. 53, 398\u2013403 (2016).","journal-title":"J. Asthma"},{"key":"23507_CR6","doi-asserted-by":"publisher","first-page":"1594","DOI":"10.1016\/j.rmed.2014.09.007","volume":"108","author":"M Leander","year":"2014","unstructured":"Leander, M. et al. Impact of anxiety and depression on respiratory symptoms. Respir Med. 108, 1594\u20131600 (2014).","journal-title":"Respir Med."},{"key":"23507_CR7","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1196\/annals.1366.028","volume":"1088","author":"FA Costa-Pinto","year":"2006","unstructured":"Costa-Pinto, F. A. et al. Neural correlates of IgE-mediated allergy. Ann. N Y Acad. Sci. 1088, 116\u2013131 (2006).","journal-title":"Ann. N Y Acad. Sci."},{"key":"23507_CR8","doi-asserted-by":"publisher","first-page":"19586","DOI":"10.1038\/s41598-019-55539-3","volume":"9","author":"K Dehdar","year":"2019","unstructured":"Dehdar, K. et al. Allergen-induced anxiety-like behavior is associated with disruption of medial prefrontal cortex - amygdala circuit. Sci. Rep. 9, 19586 (2019).","journal-title":"Sci. Rep."},{"key":"23507_CR9","doi-asserted-by":"publisher","first-page":"1070","DOI":"10.1002\/jat.2835","volume":"33","author":"TT Win-Shwe","year":"2013","unstructured":"Win-Shwe, T. T., Yanagisawa, R., Koike, E., Nitta, H. & Takano, H. Expression levels of neuroimmune biomarkers in hypothalamus of allergic mice after phthalate exposure. J. Appl. Toxicol. 33, 1070\u20131078 (2013).","journal-title":"J. Appl. Toxicol."},{"key":"23507_CR10","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1016\/j.ecoenv.2018.07.089","volume":"163","author":"J Duan","year":"2018","unstructured":"Duan, J. et al. Exposure to formaldehyde and diisononyl phthalate exacerbate neuroinflammation through NF-$\u03ba$B activation in a mouse asthma model. Ecotoxicol. Environ. Saf. 163, 356\u2013364 (2018).","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"23507_CR11","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.jneuroim.2014.06.005","volume":"273","author":"MX Xia","year":"2014","unstructured":"Xia, M. X. et al. Inhaled Budesonide protects against chronic asthma-induced neuroinflammation in mouse brain. J. Neuroimmunol. 273, 53\u201357 (2014).","journal-title":"J. Neuroimmunol."},{"key":"23507_CR12","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.jaci.2011.11.037","volume":"129","author":"SD Brown","year":"2012","unstructured":"Brown, S. D. et al. Airway TGF-\u03b21 and oxidant stress in children with severe asthma: association with airflow limitation. J. Allergy Clin. Immunol. 129, 388\u2013396 (2012).","journal-title":"J. Allergy Clin. Immunol."},{"key":"23507_CR13","doi-asserted-by":"publisher","first-page":"E32","DOI":"10.21037\/jtd.2017.01.05","volume":"9","author":"J Qu","year":"2017","unstructured":"Qu, J., Li, Y., Zhong, W., Gao, P. & Hu, C. Recent developments in the role of reactive oxygen species in allergic asthma. J. Thorac. Dis. 9, E32\u2013E43 (2017).","journal-title":"J. Thorac. Dis."},{"key":"23507_CR14","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1007\/s11011-020-00558-7","volume":"35","author":"GL Antunes","year":"2020","unstructured":"Antunes, G. L. et al. Neostigmine treatment induces neuroprotection against oxidative stress in cerebral cortex of asthmatic mice. Metab. Brain Dis. 35, 765\u2013774 (2020).","journal-title":"Metab. Brain Dis."},{"key":"23507_CR15","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1016\/j.it.2017.07.003","volume":"38","author":"A Ray","year":"2017","unstructured":"Ray, A. & Kolls, J. K. Neutrophilic inflammation in asthma and association with disease severity. Trends Immunol. 38, 942\u2013954 (2017).","journal-title":"Trends Immunol."},{"key":"23507_CR16","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-021-08894-x","author":"LO Louren\u00e7o","year":"2021","unstructured":"Louren\u00e7o, L. O. et al. Different phenotypes in asthma: clinical findings and experimental animal models. Clin. Rev. Allergy Immunol. https:\/\/doi.org\/10.1007\/s12016-021-08894-x (2021).","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"23507_CR17","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1248\/bpb.b19-00095","volume":"43","author":"T Nabe","year":"2020","unstructured":"Nabe, T. Steroid-Resistant asthma and neutrophils. Biol. Pharm. Bull. 43, 31\u201335 (2020).","journal-title":"Biol. Pharm. Bull."},{"key":"23507_CR18","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1016\/S1081-1206(10)60270-0","volume":"103","author":"S Zhang","year":"2009","unstructured":"Zhang, S., Shen, Z., Hu, G., Liu, R. & Zhang, X. Effects of endogenous glucocorticoids on allergic inflammation and TH1\/TH2 balance in airway allergic disease. Ann. Allergy Asthma Immunol. 103, 525\u2013534 (2009).","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"23507_CR19","doi-asserted-by":"publisher","first-page":"5492","DOI":"10.1523\/JNEUROSCI.0086-04.2004","volume":"24","author":"K Mizoguchi","year":"2004","unstructured":"Mizoguchi, K., Ishige, A., Takeda, S., Aburada, M. & Tabira, T. Endogenous glucocorticoids are essential for maintaining prefrontal cortical cognitive function. J. Neurosci. 24, 5492\u20135499 (2004).","journal-title":"J. Neurosci."},{"key":"23507_CR20","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.bbr.2017.02.046","volume":"326","author":"JI Caulfield","year":"2017","unstructured":"Caulfield, J. I. et al. Peri-adolescent asthma symptoms cause adult anxiety-related behavior and Neurobiological processes in mice. Behav. Brain. Res. 326, 244\u2013255 (2017).","journal-title":"Behav. Brain. Res."},{"key":"23507_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fnbeh.2018.00188","volume":"12","author":"JI Caulfield","year":"2018","unstructured":"Caulfield, J. I. et al. Asthma induction during development and adult lung function, behavior and brain gene expression. Front. Behav. Neurosci. 12, 1\u201316 (2018).","journal-title":"Front. Behav. Neurosci."},{"key":"23507_CR22","doi-asserted-by":"crossref","unstructured":"Cheng, C. et al. Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice. Biosci. Rep. 39, (2019).","DOI":"10.1042\/BSR20181117"},{"key":"23507_CR23","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1111\/j.1365-2222.2007.02670.x","volume":"37","author":"Y Riffo-Vasquez","year":"2007","unstructured":"Riffo-Vasquez, Y., Ligeiro de Oliveira, A. P., Page, C. P. & Spina, D. Tavares-de-Lima, W. Role of sex hormones in allergic inflammation in mice. Clin. Exp. Allergy. 37, 459\u2013470 (2007).","journal-title":"Clin. Exp. Allergy"},{"key":"23507_CR24","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/S0165-0270(00)00289-2","volume":"102","author":"RH Silva","year":"2000","unstructured":"Silva, R. H. & Frussa-Filho, R. The plus-maze discriminative avoidance task: A new model to study memory-anxiety interactions. Effects of chlordiazepoxide and caffeine. J. Neurosci. Methods. 102, 117\u2013125 (2000).","journal-title":"J. Neurosci. Methods"},{"key":"23507_CR25","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.pbb.2015.11.012","volume":"141","author":"AHFF Le\u00e3o","year":"2016","unstructured":"Le\u00e3o, A. H. F. F., Cabral, A., Iz\u00eddio, G. S., Ribeiro, A. M. & Silva, R. H. Diazepam effects on aversive memory retrieval and extinction: role of anxiety levels. Pharmacol. Biochem. Behav. 141, 42\u201349 (2016).","journal-title":"Pharmacol. Biochem. Behav."},{"key":"23507_CR26","doi-asserted-by":"crossref","unstructured":"Toledo, A. C. et al. Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model. Br J. Pharmacol. 168, (2013).","DOI":"10.1111\/bph.12062"},{"key":"23507_CR27","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/S0076-6879(84)05013-8","volume":"105","author":"L Floh\u00e9","year":"1984","unstructured":"Floh\u00e9, L. & \u00f6tting, F. Superoxide dismutase assays. Oxygen radicals in biological systems. Methods Enzymol. 105, 93\u2013104 (1984).","journal-title":"Methods Enzymol."},{"key":"23507_CR28","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/S0076-6879(84)05015-1","volume":"105","author":"L Floh\u00e9","year":"1984","unstructured":"Floh\u00e9, L. & G\u00fcnzler, W. A. Assays of glutathione peroxidase. Oxygen radicals in biological systems. Methods Enzymol. 105, 114\u2013120 (1984).","journal-title":"Methods Enzymol."},{"key":"23507_CR29","doi-asserted-by":"publisher","first-page":"2407","DOI":"10.4049\/jimmunol.141.7.2407","volume":"141","author":"AH Ding","year":"1988","unstructured":"Ding, A. H., Nathan, C. F. & Stuehr, D. J. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 141, 2407\u20132412 (1988).","journal-title":"J. Immunol."},{"key":"23507_CR30","doi-asserted-by":"crossref","unstructured":"Ponci, V. et al. Biseugenol exhibited anti-inflammatory and anti-asthmatic effects in an asthma mouse model of mixed-granulocytic asthma. Molecules 25, (2020).","DOI":"10.3390\/molecules25225384"},{"key":"23507_CR31","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.fct.2018.11.056","volume":"124","author":"JIA Beserra-Filho","year":"2019","unstructured":"Beserra-Filho, J. I. A. et al. Eplingiella fruticosa leaf essential oil complexed with \u03b2-cyclodextrin produces a superior neuroprotective and behavioral profile in a mice model of parkinson\u2019s disease. Food Chem. Toxicol. 124, 17\u201329 (2019).","journal-title":"Food Chem. Toxicol."},{"key":"23507_CR32","doi-asserted-by":"publisher","first-page":"1488","DOI":"10.1111\/1440-1681.13563","volume":"48","author":"S Silva-Martins","year":"2021","unstructured":"Silva-Martins, S. et al. Myrtenol complexed with \u03b2\u2010cyclodextrin ameliorates behavioural deficits and reduces oxidative stress in the reserpine\u2010induced animal model of parkinsonism. Clin. Exp. Pharmacol. Physiol. 48, 1488\u20131499 (2021).","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"23507_CR33","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.coi.2018.05.006","volume":"54","author":"C Radermecker","year":"2018","unstructured":"Radermecker, C., Louis, R., Bureau, F. & Marichal, T. Role of neutrophils in allergic asthma. Curr. Opin. Immunol. 54, 28\u201334 (2018).","journal-title":"Curr. Opin. Immunol."},{"key":"23507_CR34","doi-asserted-by":"publisher","first-page":"1725","DOI":"10.1152\/japplphysiol.01018.2018","volume":"126","author":"T Ritz","year":"2019","unstructured":"Ritz, T. et al. Central nervous system signatures of affect in asthma: associations with emotion-induced bronchoconstriction, airway inflammation, and asthma control. J. Appl. Physiol. (1985). 126, 1725\u20131736 (2019).","journal-title":"J. Appl. Physiol. (1985)"},{"key":"23507_CR35","doi-asserted-by":"crossref","unstructured":"Sakoda, C. P. P. et al. Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation. Acta Histochem. 118, (2016).","DOI":"10.1016\/j.acthis.2016.07.001"},{"key":"23507_CR36","doi-asserted-by":"publisher","first-page":"173239","DOI":"10.1016\/j.ejphar.2020.173239","volume":"882","author":"NM Pinheiro","year":"2020","unstructured":"Pinheiro, N. M. et al. Effects of VAChT reduction and \u03b17nAChR stimulation by PNU-282987 in lung inflammation in a model of chronic allergic airway inflammation. Eur. J. Pharmacol. 882, 173239 (2020).","journal-title":"Eur. J. Pharmacol."},{"key":"23507_CR37","doi-asserted-by":"publisher","unstructured":"Holgate, S. T. et al. Asthma. Nat. Publishing Group. 1\u201322. https:\/\/doi.org\/10.1038\/nrdp.2015.25 (2015).","DOI":"10.1038\/nrdp.2015.25"},{"key":"23507_CR38","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/s12016-019-08736-x","volume":"57","author":"M Barnthouse","year":"2019","unstructured":"Barnthouse, M. & Jones, B. L. The impact of environmental chronic and toxic stress on asthma. Clin. Rev. Allergy Immunol. 57, 427\u2013438 (2019).","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"23507_CR39","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1007\/s00011-009-0054-2","volume":"58","author":"MM Gueders","year":"2009","unstructured":"Gueders, M. M. et al. Mouse models of asthma: a comparison between C57BL\/6 and BALB\/c strains regarding bronchial responsiveness, inflammation, and cytokine production. Inflamm. Res. 58, 845\u2013854 (2009).","journal-title":"Inflamm. Res."},{"key":"23507_CR40","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1016\/j.jaci.2018.09.011","volume":"142","author":"C Nassenstein","year":"2018","unstructured":"Nassenstein, C., Krasteva-Christ, G. & Renz, H. New aspects of neuroinflammation and neuroimmune crosstalk in the airways. J. Allergy Clin. Immunol. 142, 1415\u20131422 (2018).","journal-title":"J. Allergy Clin. Immunol."},{"key":"23507_CR41","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1038\/s41577-018-0006-6","volume":"18","author":"PJ Barnes","year":"2018","unstructured":"Barnes, P. J. Targeting cytokines to treat asthma and chronic obstructive pulmonary disease. Nat. Rev. Immunol. 18, 454\u2013466 (2018).","journal-title":"Nat. Rev. Immunol."},{"key":"23507_CR42","doi-asserted-by":"publisher","first-page":"108606","DOI":"10.1016\/j.intimp.2022.108606","volume":"106","author":"X Wen","year":"2022","unstructured":"Wen, X. et al. Changes in the phenotype and function of mucosal-associated invariant T cells in neutrophilic asthma. Int. Immunopharmacol. 106, 108606 (2022).","journal-title":"Int. Immunopharmacol."},{"key":"23507_CR43","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1038\/nm.2731","volume":"18","author":"ST Holgate","year":"2012","unstructured":"Holgate, S. T. Innate and adaptive immune responses in asthma. Nat. Med. 18, 673\u2013683 (2012).","journal-title":"Nat. Med."},{"key":"23507_CR44","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1016\/j.jaci.2017.02.045","volume":"141","author":"C Rossios","year":"2018","unstructured":"Rossios, C. et al. Sputum transcriptomics reveal upregulation of IL-1 receptor family members in patients with severe asthma. J. Allergy Clin. Immunol. 141, 560\u2013570 (2018).","journal-title":"J. Allergy Clin. Immunol."},{"key":"23507_CR45","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1007\/s12026-015-8718-1","volume":"63","author":"VA Pavlov","year":"2015","unstructured":"Pavlov, V. A. & Tracey, K. J. Neural circuitry and immunity. Immunol. Res. 63, 38\u201357 (2015).","journal-title":"Immunol. Res."},{"key":"23507_CR46","doi-asserted-by":"publisher","first-page":"601","DOI":"10.2119\/molmed.2014.00147","volume":"20","author":"HA Silverman","year":"2015","unstructured":"Silverman, H. A. et al. Brain region-specific alterations in the gene expression of cytokines, immune cell markers and cholinergic system components during peripheral endotoxin-induced inflammation. Mol. Med. 20, 601\u2013611 (2015).","journal-title":"Mol. Med."},{"key":"23507_CR47","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1002\/JLB.1RU1117-428R","volume":"103","author":"SA Sonar","year":"2018","unstructured":"Sonar, S. A. & Lal, G. Blood\u2013brain barrier and its function during inflammation and autoimmunity. J. Leukoc. Biol. 103, 839\u2013853 (2018).","journal-title":"J. Leukoc. Biol."},{"key":"23507_CR48","doi-asserted-by":"publisher","first-page":"482","DOI":"10.2500\/aap.2009.30.3264","volume":"30","author":"P Lieberman","year":"2009","unstructured":"Lieberman, P. Histamine, antihistamines, and the central nervous system. Allergy Asthma Proc. 30, 482\u2013486 (2009).","journal-title":"Allergy Asthma Proc."},{"key":"23507_CR49","first-page":"191","volume":"1","author":"D Paredes","year":"2010","unstructured":"Paredes, D., Acosta, S., Gemma, C. & Bickford, P. C. Role of TNF$\u03b1$ induced inflammation in delay eyeblink conditioning in young and aged rats. Aging Dis. 1, 191\u2013198 (2010).","journal-title":"Aging Dis."},{"key":"23507_CR50","doi-asserted-by":"publisher","unstructured":"Wang, Z., Pu, Q., Huang, C. & Wu, M. 333\u2013350.  https:\/\/doi.org\/10.1007\/978-3-030-63046-1_18 (2021).","DOI":"10.1007\/978-3-030-63046-1_18"},{"key":"23507_CR51","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1007\/s11011-022-00907-8","volume":"37","author":"GL Antunes","year":"2022","unstructured":"Antunes, G. L. et al. Airway inflammation induces anxiety-like behavior through neuroinflammatory, neurochemical, and neurometabolic changes in an allergic asthma model. Metab. Brain Dis. 37, 911\u2013926 (2022).","journal-title":"Metab. Brain Dis."},{"key":"23507_CR52","doi-asserted-by":"publisher","unstructured":"Antunes, G. L. et al. Cholinergic anti-inflammatory pathway confers airway protection against oxidative damage and attenuates inflammation in an allergic asthma model. J. Cell. Physiol. 1\u201312 https:\/\/doi.org\/10.1002\/jcp.29101 (2019).","DOI":"10.1002\/jcp.29101"},{"key":"23507_CR53","doi-asserted-by":"crossref","unstructured":"Mokhtari-Zaer, A., Hosseini, M. & Boskabady, M. H. The effects of exercise on depressive- and anxiety-like behaviors as well as lung and hippocampus oxidative stress in ovalbumin-sensitized juvenile rats. Respir.  Physiol. Neurobiol. 248, (2018).","DOI":"10.1016\/j.resp.2017.12.001"},{"key":"23507_CR54","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1089\/ars.2007.1937","volume":"10","author":"H Sugiura","year":"2008","unstructured":"Sugiura, H. & Ichinose, M. Oxidative and nitrative stress in bronchial asthma. Antioxid. Redox Signal. 10, 785\u2013798 (2008).","journal-title":"Antioxid. Redox Signal."},{"key":"23507_CR55","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1101\/lm.813608","volume":"15","author":"JJ Quinn","year":"2008","unstructured":"Quinn, J. J., Ma, Q. D., Tinsley, M. R., Koch, C. & Fanselow, M. S. Inverse Temporal contributions of the dorsal hippocampus and medial prefrontal cortex to the expression of long-term fear memories. Learn. Mem. 15, 368\u2013372 (2008).","journal-title":"Learn. Mem."},{"key":"23507_CR56","doi-asserted-by":"publisher","first-page":"17415","DOI":"10.1074\/jbc.M112400200","volume":"277","author":"ML Brennan","year":"2002","unstructured":"Brennan, M. L. et al. A Tale of two controversies: defining both the role of peroxidases in Nitrotyrosine formation in vivo using eosinophil peroxidase and myeloperoxidase-deficient mice, and the nature of peroxidase-generated reactive nitrogen species. J. Biol. Chem. 277, 17415\u201317427 (2002).","journal-title":"J. Biol. Chem."},{"key":"23507_CR57","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1038\/nrn2214","volume":"8","author":"V Calabrese","year":"2007","unstructured":"Calabrese, V. et al. Nitric oxide in the central nervous system: neuroprotection versus neurotoxicity. Nat. Rev. Neurosci. 8, 766\u2013775 (2007).","journal-title":"Nat. Rev. Neurosci."},{"key":"23507_CR58","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1002\/ana.23842","volume":"73","author":"C Charriaut-Marlangue","year":"2013","unstructured":"Charriaut-Marlangue, C. et al. Nitric oxide signaling in the brain: A new target for inhaled nitric oxide? Ann. Neurol. 73, 442\u2013448 (2013).","journal-title":"Ann. Neurol."},{"key":"23507_CR59","doi-asserted-by":"crossref","unstructured":"Prado, C. M. et al. Effects of nitric oxide synthases in chronic allergic airway inflammation and remodeling. Am J. Respir Cell. Mol. Biol. 35, (2006).","DOI":"10.1165\/rcmb.2005-0391OC"},{"key":"23507_CR60","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1016\/j.chest.2021.10.015","volume":"161","author":"H Rupani","year":"2022","unstructured":"Rupani, H. & Kent, B. D. Using fractional exhaled nitric oxide measurement in clinical asthma management. Chest 161, 906\u2013917 (2022).","journal-title":"Chest"},{"key":"23507_CR61","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.brainres.2006.09.008","volume":"1122","author":"RC Carvalho","year":"2006","unstructured":"Carvalho, R. C. et al. Effects of reserpine on the plus-maze discriminative avoidance task: dissociation between memory and motor impairments. Brain Res. 1122, 179\u2013183 (2006).","journal-title":"Brain Res."},{"key":"23507_CR62","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1017\/neu.2015.16","volume":"27","author":"HA Fernandes","year":"2015","unstructured":"Fernandes, H. A. et al. Effects of post-training modafinil administration in a discriminative avoidance task in mice. Acta Neuropsychiatr. 27, 235\u2013241 (2015).","journal-title":"Acta Neuropsychiatr"},{"key":"23507_CR63","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/s00213-006-0684-9","volume":"192","author":"SR Kameda","year":"2007","unstructured":"Kameda, S. R. et al. Dissociation of the effects of ethanol on memory, anxiety, and motor behavior in mice tested in the plus-maze discriminative avoidance task. Psychopharmacol. (Berl). 192, 39\u201348 (2007).","journal-title":"Psychopharmacol. (Berl)"},{"key":"23507_CR64","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/s00213-001-0948-3","volume":"160","author":"R Silva","year":"2002","unstructured":"Silva, R. et al. Effects of amphetamine on the plus-maze discriminative avoidance task in mice. Psychopharmacol. (Berl). 160, 9\u201318 (2002).","journal-title":"Psychopharmacol. (Berl)"},{"key":"23507_CR65","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.nlm.2014.08.008","volume":"115","author":"EJ Donzis","year":"2014","unstructured":"Donzis, E. J. & Tronson, N. C. Modulation of learning and memory by cytokines: signaling mechanisms and long term consequences. Neurobiol. Learn. Mem. 115, 68\u201377 (2014).","journal-title":"Neurobiol. Learn. Mem."},{"key":"23507_CR66","doi-asserted-by":"publisher","first-page":"1103","DOI":"10.1016\/j.molmed.2017.10.006","volume":"23","author":"L Ulloa","year":"2017","unstructured":"Ulloa, L., Quiroz-Gonzalez, S. & Torres-Rosas, R. Nerve stimulation: Immunomodulation and control of inflammation. Trends Mol. Med. 23, 1103\u20131120 (2017).","journal-title":"Trends Mol. Med."},{"key":"23507_CR67","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.brainresbull.2020.09.016","volume":"165","author":"A Mokhtari-Zaer","year":"2020","unstructured":"Mokhtari-Zaer, A., Hosseini, M., Roshan, N. M. & Boskabady, M. H. Treadmill exercise ameliorates memory deficits and hippocampal inflammation in ovalbumin-sensitized juvenile rats. Brain Res. Bull. 165, 40\u201347 (2020).","journal-title":"Brain Res. Bull."},{"key":"23507_CR68","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s12979-022-00299-4","volume":"19","author":"TE Hodges","year":"2022","unstructured":"Hodges, T. E., Lieblich, S. E., Rechlin, R. K. & Galea, L. A. M. Sex differences in inflammation in the hippocampus and amygdala across the lifespan in rats: associations with cognitive bias. Immun. Ageing. 19, 43 (2022).","journal-title":"Immun. Ageing"},{"key":"23507_CR69","doi-asserted-by":"publisher","first-page":"5288","DOI":"10.3390\/ijms26115288","volume":"26","author":"R Borrelli","year":"2025","unstructured":"Borrelli, R. et al. Sex-Based differences in asthma: Pathophysiology, hormonal Influence, and genetic mechanisms. Int. J. Mol. Sci. 26, 5288 (2025).","journal-title":"Int. J. Mol. Sci."},{"key":"23507_CR70","doi-asserted-by":"publisher","first-page":"210067","DOI":"10.1183\/16000617.0067-2021","volume":"30","author":"NU Chowdhury","year":"2021","unstructured":"Chowdhury, N. U., Guntur, V. P., Newcomb, D. C. & Wechsler, M. E. Sex and gender in asthma. Eur. Respiratory Rev. 30, 210067 (2021).","journal-title":"Eur. Respiratory Rev."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-23507-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-23507-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-23507-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T23:03:19Z","timestamp":1763074999000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-23507-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,13]]},"references-count":70,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["23507"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-23507-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,13]]},"assertion":[{"value":"13 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 October 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"39804"}}