{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T10:56:14Z","timestamp":1763636174933,"version":"3.45.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K07500","22H03138","23K08038","22H03138","23K08038","19K07500"],"award-info":[{"award-number":["19K07500","22H03138","23K08038","22H03138","23K08038","19K07500"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["22hma322002h0001","22hma322002h0001","22hma322002h0001","22hma322002h0001"],"award-info":[{"award-number":["22hma322002h0001","22hma322002h0001","22hma322002h0001","22hma322002h0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-24998-2","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T10:51:20Z","timestamp":1763635880000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ethylenediaminetetraacetic acid conjugated with polyethylene glycol is a safe chelator for colonic epithelial denudation and intestinal epithelial replacement therapy"],"prefix":"10.1038","volume":"15","author":[{"given":"Yuka","family":"Matsumoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuto","family":"Suda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirei","family":"Takahasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichiro","family":"Nakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nozomi","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shihei","family":"Motofuji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Go","family":"Miyano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Koga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobutaka","family":"Hattori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiichiro","family":"Tsuchiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuyuki","family":"Yamataka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"24998_CR1","doi-asserted-by":"publisher","first-page":"14S","DOI":"10.1177\/0148607113520005","volume":"38","author":"KA Tappenden","year":"2014","unstructured":"Tappenden, K. A. Pathophysiology of short bowel syndrome: considerations of resected and residual anatomy. JPEN J. Parenter. Enter. Nutr. 38, 14S\u201322S. https:\/\/doi.org\/10.1177\/0148607113520005 (2014).","journal-title":"JPEN J. Parenter. Enter. Nutr."},{"key":"24998_CR2","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.bpg.2016.02.011","volume":"30","author":"L Pironi","year":"2016","unstructured":"Pironi, L. Definitions of intestinal failure and the short bowel syndrome. Best Pract. Res. Clin. Gastroenterol. 30, 173\u2013185. https:\/\/doi.org\/10.1016\/j.bpg.2016.02.011 (2016).","journal-title":"Best Pract. Res. Clin. Gastroenterol."},{"key":"24998_CR3","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/s0261-5614(03)00042-6","volume":"22","author":"E Carlsson","year":"2003","unstructured":"Carlsson, E., Bosaeus, I. & Nordgren, S. Quality of life and concerns in patients with short bowel syndrome. Clin. Nutr. 22, 445\u2013452. https:\/\/doi.org\/10.1016\/s0261-5614(03)00042-6 (2003).","journal-title":"Clin. Nutr."},{"key":"24998_CR4","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1080\/17474124.2019.1569514","volume":"13","author":"L Billiauws","year":"2019","unstructured":"Billiauws, L. & Joly, F. Emerging treatments for short bowel syndrome in adult patients. Expert Rev. Gastroenterol. Hepatol. 13, 241\u2013246. https:\/\/doi.org\/10.1080\/17474124.2019.1569514 (2019).","journal-title":"Expert Rev. Gastroenterol. Hepatol."},{"key":"24998_CR5","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1038\/nature07935","volume":"459","author":"T Sato","year":"2009","unstructured":"Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262\u2013265. https:\/\/doi.org\/10.1038\/nature07935 (2009).","journal-title":"Nature"},{"key":"24998_CR6","doi-asserted-by":"publisher","first-page":"G1","DOI":"10.1152\/ajpgi.00425.2020","volume":"321","author":"M Kasendra","year":"2021","unstructured":"Kasendra, M. et al. Intestinal organoids: roadmap to the clinic. Am. J. Physiol. Gastrointest. Liver Physiol. 321, G1\u2013G10. https:\/\/doi.org\/10.1152\/ajpgi.00425.2020 (2021).","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"24998_CR7","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1016\/S2352-4642(22)00193-6","volume":"6","author":"PKH Tam","year":"2022","unstructured":"Tam, P. K. H. et al. Regenerative medicine: postnatal approaches. Lancet Child. Adolesc. Health. 6, 654\u2013666. https:\/\/doi.org\/10.1016\/S2352-4642(22)00193-6 (2022).","journal-title":"Lancet Child. Adolesc. Health"},{"key":"24998_CR8","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.jcmgh.2017.10.006","volume":"5","author":"T Nakamura","year":"2018","unstructured":"Nakamura, T. & Sato, T. Advancing intestinal organoid technology toward regenerative medicine. Cell. Mol. Gastroenterol. Hepatol. 5, 51\u201360. https:\/\/doi.org\/10.1016\/j.jcmgh.2017.10.006 (2018).","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"24998_CR9","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/s41575-022-00586-x","volume":"19","author":"L Tullie","year":"2022","unstructured":"Tullie, L., Jones, B. C., De Coppi, P. & Li, V. S. W. Building gut from scratch - progress and update of intestinal tissue engineering. Nat. Rev. Gastroenterol. Hepatol. 19, 417\u2013431. https:\/\/doi.org\/10.1038\/s41575-022-00586-x (2022).","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"24998_CR10","doi-asserted-by":"publisher","first-page":"1752","DOI":"10.1101\/gad.245233.114","volume":"28","author":"M Fukuda","year":"2014","unstructured":"Fukuda, M. et al. Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon. Genes Dev. 28, 1752\u20131757. https:\/\/doi.org\/10.1101\/gad.245233.114 (2014).","journal-title":"Genes Dev."},{"key":"24998_CR11","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1038\/s41586-021-03247-2","volume":"592","author":"S Sugimoto","year":"2021","unstructured":"Sugimoto, S. et al. Anorganoid-based organ-repurposing approach to treat short bowel syndrome. Nature 592, 99\u2013104. https:\/\/doi.org\/10.1038\/s41586-021-03247-2 (2021).","journal-title":"Nature"},{"key":"24998_CR12","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/j.tcb.2021.05.002","volume":"31","author":"J Huang","year":"2021","unstructured":"Huang, J., Xu, Z. & Ren, J. Small intestinalization of colon using ileum organoids. Trends Cell. Biol. 31, 517\u2013519. https:\/\/doi.org\/10.1016\/j.tcb.2021.05.002 (2021).","journal-title":"Trends Cell. Biol."},{"key":"24998_CR13","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/0092-8674(87)90233-9","volume":"48","author":"RO Hynes","year":"1987","unstructured":"Hynes, R. O. Integrins: a family of cell surface receptors. Cell 48, 549\u2013554. https:\/\/doi.org\/10.1016\/0092-8674(87)90233-9 (1987).","journal-title":"Cell"},{"key":"24998_CR14","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1126\/science.2821619","volume":"238","author":"E Ruoslahti","year":"1987","unstructured":"Ruoslahti, E. & Pierschbacher, M. D. New perspectives in cell adhesion: RGD and integrins. Science 238, 491\u2013497. https:\/\/doi.org\/10.1126\/science.2821619 (1987).","journal-title":"Science"},{"key":"24998_CR15","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1126\/science.2392682","volume":"249","author":"JC Loftus","year":"1990","unstructured":"Loftus, J. C. et al. A beta 3 integrin mutation abolishes ligand binding and alters divalent cation-dependent conformation. Science 249, 915\u2013918. https:\/\/doi.org\/10.1126\/science.2392682 (1990).","journal-title":"Science"},{"key":"24998_CR16","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1080\/10915810290096522","volume":"21 Suppl 2","author":"RS Lanigan","year":"2002","unstructured":"Lanigan, R. S. & Yamarik, T. A. Final report on the safety assessment of EDTA, calcium disodium EDTA, diammonium EDTA, dipotassium EDTA, disodium EDTA, TEA-EDTA, tetrasodium EDTA, tripotassium EDTA, trisodium EDTA, HEDTA, and trisodium HEDTA. Int. J. Toxicol. 21 Suppl 2, 95\u2013142. https:\/\/doi.org\/10.1080\/10915810290096522 (2002).","journal-title":"Int. J. Toxicol."},{"key":"24998_CR17","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/0300-9629(94)00156-n","volume":"110","author":"DJ Desmecht","year":"1995","unstructured":"Desmecht, D. J., Linden, A. S., Godeau, J. M. & Lekeux, P. M. Experimental production of hypocalcemia by EDTA infusion in calves: a critical appraisal assessed from the profile of blood chemicals and enzymes. Comp. Biochem. Physiol. Physiol. 110, 115\u2013130. https:\/\/doi.org\/10.1016\/0300-9629(94)00156-n (1995).","journal-title":"Comp. Biochem. Physiol. Physiol."},{"key":"24998_CR18","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1113\/jphysiol.1964.sp007353","volume":"170","author":"JM PAYNE","year":"1964","unstructured":"Payne, J. M., Sansom, B. F., The relative toxicity in & rats of disodium ethylene diamine tetra-acetate, sodium oxalate and sodium citrate. J. Physiol. 170, 613\u2013620, doi:https:\/\/doi.org\/10.1113\/jphysiol.1964.sp007353 (1964).","journal-title":"J. Physiol."},{"key":"24998_CR19","doi-asserted-by":"publisher","first-page":"908","DOI":"10.1002\/jps.2600810914","volume":"81","author":"R Mehvar","year":"1992","unstructured":"Mehvar, R. & Shepard, T. L. Molecular-weight-dependent pharmacokinetics of fluorescein-labeled dextrans in rats. J. Pharm. Sci. 81, 908\u2013912. https:\/\/doi.org\/10.1002\/jps.2600810914 (1992).","journal-title":"J. Pharm. Sci."},{"key":"24998_CR20","doi-asserted-by":"publisher","first-page":"R597","DOI":"10.1152\/ajpregu.1984.246.4.R597","volume":"246","author":"MF Flessner","year":"1984","unstructured":"Flessner, M. F., Dedrick, R. L. & Schultz, J. S. A distributed model of peritoneal-plasma transport: theoretical considerations. Am. J. Physiol. 246, R597\u2013607. https:\/\/doi.org\/10.1152\/ajpregu.1984.246.4.R597 (1984).","journal-title":"Am. J. Physiol."},{"key":"24998_CR21","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/s0169-409x(00)00129-0","volume":"46","author":"CA Lipinski","year":"2001","unstructured":"Lipinski, C. A., Lombardo, F., Dominy, B. W. & Feeney, P. J. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Adv. Drug Deliv Rev. 46, 3\u201326. https:\/\/doi.org\/10.1016\/s0169-409x(00)00129-0 (2001).","journal-title":"Adv. Drug Deliv Rev."},{"key":"24998_CR22","doi-asserted-by":"publisher","first-page":"776","DOI":"10.2174\/1568026611313070002","volume":"13","author":"KJ Filipski","year":"2013","unstructured":"Filipski, K. J. et al. Intestinal targeting of drugs: rational design approaches and challenges. Curr. Top. Med. Chem. 13, 776\u2013802. https:\/\/doi.org\/10.2174\/1568026611313070002 (2013).","journal-title":"Curr. Top. Med. Chem."},{"key":"24998_CR23","doi-asserted-by":"publisher","first-page":"1434","DOI":"10.2174\/138161212799504858","volume":"18","author":"D Charmot","year":"2012","unstructured":"Charmot, D. Non-systemic drugs: a critical review. Curr. Pharm. Des. 18, 1434\u20131445. https:\/\/doi.org\/10.2174\/138161212799504858 (2012).","journal-title":"Curr. Pharm. Des."},{"key":"24998_CR24","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/0016-5085(80)90071-2","volume":"79","author":"GR Davis","year":"1980","unstructured":"Davis, G. R., Ana, S., Morawski, C. A., Fordtran, J. S. & S. G. & Inhibition of water and electrolyte absorption by polyethylene glycol (PEG). Gastroenterology 79, 35\u201339 (1980).","journal-title":"Gastroenterology"},{"key":"24998_CR25","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1002\/jps.3030360507","volume":"36","author":"CB SHAFFER","year":"1947","unstructured":"Shaffer, C. B. & Critchfield, F. H. The absorption and excretion of the solid polyethylene glycols; (carbowax compounds). J. Am. Pharm. Assoc. Am. Pharm. Assoc. 36, 152\u2013157. https:\/\/doi.org\/10.1002\/jps.3030360507 (1947).","journal-title":"J. Am. Pharm. Assoc. Am. Pharm. Assoc."},{"key":"24998_CR26","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1002\/jps.3030390613","volume":"39","author":"CB SHAFFER","year":"1950","unstructured":"Shaffer, C. B., Nair, J. H. & Critchfield, F. H. & The absorption and excretion of a liquid polyethylene glycol. J. Am. Pharm. Assoc. Am. Pharm. Assoc. 39, 340\u2013344. https:\/\/doi.org\/10.1002\/jps.3030390613 (1950).","journal-title":"J. Am. Pharm. Assoc. Am. Pharm. Assoc."},{"key":"24998_CR27","doi-asserted-by":"publisher","first-page":"1593","DOI":"10.1021\/acs.jmedchem.0c01774","volume":"64","author":"V Hoguet","year":"2021","unstructured":"Hoguet, V. et al. Beyond the rule of 5: impact of pegylation with various polymer sizes on Pharmacokinetic Properties, Structure-Properties relationships of mPEGylated small agonists of TGR5 receptor. J. Med. Chem. 64, 1593\u20131610. https:\/\/doi.org\/10.1021\/acs.jmedchem.0c01774 (2021).","journal-title":"J. Med. Chem."},{"key":"24998_CR28","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.aca.2016.04.019","volume":"924","author":"S Karita","year":"2016","unstructured":"Karita, S. & Kaneta, T. Chelate titrations of Ca(2+) and Mg(2+) using microfluidic paper-based analytical devices. Anal. Chim. Acta. 924, 60\u201367. https:\/\/doi.org\/10.1016\/j.aca.2016.04.019 (2016).","journal-title":"Anal. Chim. Acta"},{"key":"24998_CR29","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1007\/s00383-021-04957-4","volume":"37","author":"Y Matsumoto","year":"2021","unstructured":"Matsumoto, Y. et al. Defined serum-free culture of human infant small intestinal organoids with predetermined doses of Wnt3a and R-spondin1 from surgical specimens. Pediatr. Surg. Int. 37, 1543\u20131554. https:\/\/doi.org\/10.1007\/s00383-021-04957-4 (2021).","journal-title":"Pediatr. Surg. Int."},{"key":"24998_CR30","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1007\/s00535-018-1437-3","volume":"53","author":"K Suzuki","year":"2018","unstructured":"Suzuki, K. et al. Single cell analysis of crohn\u2019s disease patient-derived small intestinal organoids reveals disease activity-dependent modification of stem cell properties. J. Gastroenterol. 53, 1035\u20131047. https:\/\/doi.org\/10.1007\/s00535-018-1437-3 (2018).","journal-title":"J. Gastroenterol."},{"key":"24998_CR31","doi-asserted-by":"publisher","unstructured":"Colombo, F. et al. Fluctuations of dry and total mass of cells exposed to different molecular weights of polyethylene glycol. Adv. NanoBiomed Res. 3 https:\/\/doi.org\/10.1002\/anbr.202200156 (2023).","DOI":"10.1002\/anbr.202200156"},{"key":"24998_CR32","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1016\/S0021-9258(19)52375-4","volume":"203","author":"H FOREMAN","year":"1953","unstructured":"Foreman, H., Vier, M. & Magee, M. The metabolism of C14-labeled Ethylenediaminetetraacetic acid in the rat. J. Biol. Chem. 203, 1045\u20131053 (1953).","journal-title":"J. Biol. Chem."},{"key":"24998_CR33","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1248\/bpb.b13-00730","volume":"37","author":"H Hirata","year":"2014","unstructured":"Hirata, H. et al. Novel diagnostic method of peritoneal injury using dual macromolecular markers. Biol. Pharm. Bull. 37, 262\u2013267. https:\/\/doi.org\/10.1248\/bpb.b13-00730 (2014).","journal-title":"Biol. Pharm. Bull."},{"key":"24998_CR34","doi-asserted-by":"publisher","first-page":"1914","DOI":"10.1016\/0016-5085(86)90261-1","volume":"90","author":"CE Brady","year":"1986","unstructured":"Brady, C. E., DiPalma, J. A., Morawski, S. G., Ana, S., Fordtran, J. S. & C. A. & Urinary excretion of polyethylene glycol 3350 and sulfate after gut lavage with a polyethylene glycol electrolyte lavage solution. Gastroenterology 90, 1914\u20131918. https:\/\/doi.org\/10.1016\/0016-5085(86)90261-1 (1986).","journal-title":"Gastroenterology"},{"key":"24998_CR35","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.stem.2017.11.012","volume":"22","author":"S Sugimoto","year":"2018","unstructured":"Sugimoto, S. et al. Reconstruction of the human colon epithelium in vivo. Cell. Stem Cell. 22, 171\u2013176e175. https:\/\/doi.org\/10.1016\/j.stem.2017.11.012 (2018).","journal-title":"Cell. Stem Cell."},{"key":"24998_CR36","doi-asserted-by":"publisher","first-page":"G63","DOI":"10.1152\/ajpgi.00112.2013","volume":"308","author":"LM Gonzalez","year":"2015","unstructured":"Gonzalez, L. M., Moeser, A. J. & Blikslager, A. T. Animal models of ischemia-reperfusion-induced intestinal injury: progress and promise for translational research. Am. J. Physiol. Gastrointest. Liver Physiol. 308, G63\u201375. https:\/\/doi.org\/10.1152\/ajpgi.00112.2013 (2015).","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"24998_CR37","doi-asserted-by":"publisher","first-page":"750731","DOI":"10.3389\/fmed.2021.750731","volume":"8","author":"G Li","year":"2021","unstructured":"Li, G., Wang, S. & Fan, Z. Oxidative stress in intestinal Ischemia-Reperfusion. Front. Med. (Lausanne). 8, 750731. https:\/\/doi.org\/10.3389\/fmed.2021.750731 (2021).","journal-title":"Front. Med. (Lausanne)"},{"key":"24998_CR38","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1248\/bpb.b13-00107","volume":"36","author":"T Ishida","year":"2013","unstructured":"Ishida, T. & Kiwada, H. Anti-polyethyleneglycol antibody response to pegylated substances. Biol. Pharm. Bull. 36, 889\u2013891. https:\/\/doi.org\/10.1248\/bpb.b13-00107 (2013).","journal-title":"Biol. Pharm. Bull."},{"key":"24998_CR39","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/s0014-5793(97)00313-x","volume":"407","author":"M Okabe","year":"1997","unstructured":"Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T. & Nishimune, Y. Green mice\u2019 as a source of ubiquitous green cells. FEBS Lett. 407, 313\u2013319. https:\/\/doi.org\/10.1016\/s0014-5793(97)00313-x (1997).","journal-title":"FEBS Lett."},{"key":"24998_CR40","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/S0006-3495(79)85322-9","volume":"25","author":"MV Thomas","year":"1979","unstructured":"Thomas, M. V. & ArsenazoIII Forms 2:1 complexes with Ca and 1:1 complexes with Mg under physiological conditions. Estimates of the apparent dissociation constants. Biophys. J. 25, 541\u2013548. https:\/\/doi.org\/10.1016\/S0006-3495(79)85322-9 (1979).","journal-title":"Biophys. J."},{"key":"24998_CR41","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1007\/s12020-020-02324-2","volume":"69","author":"J Pepe","year":"2020","unstructured":"Pepe, J. et al. Diagnosis and management of hypocalcemia. Endocrine 69, 485\u2013495. https:\/\/doi.org\/10.1007\/s12020-020-02324-2 (2020).","journal-title":"Endocrine"},{"key":"24998_CR42","doi-asserted-by":"publisher","first-page":"h2723","DOI":"10.1136\/bmj.h2723","volume":"350","author":"S Minisola","year":"2015","unstructured":"Minisola, S., Pepe, J., Piemonte, S. & Cipriani, C. The diagnosis and management of hypercalcaemia. BMJ 350, h2723. https:\/\/doi.org\/10.1136\/bmj.h2723 (2015).","journal-title":"BMJ"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-24998-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-24998-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-24998-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T10:51:21Z","timestamp":1763635881000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-24998-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,20]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["24998"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-24998-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,20]]},"assertion":[{"value":"26 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 October 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Y. Matsumoto, TN, Y. Murakami K.N., N.O., and S.M. are inventors of a product whose patent is under application (Patent Application No: PCT\/JP2021\/047282). K.S., M.T., G.M., H.K., N.H., K.T., and A.Y. declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"41025"}}