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However, severe infestations can cause intestinal impaction, colic, unthriftiness, peritonitis, and small intestine rupture, resulting in death. Parasite resistance to conventional drugs is mainly caused by decreased drug absorption and increased metabolism. Nanoparticle-based drugs can be viable alternatives. The study studied the pharmacological effects of zinc oxide nanoparticles( ZnO NPs) on\n                    <jats:italic>P. equorum<\/jats:italic>\n                    , infected rats\u2019 lungs and spleens. Thirty-six male rats were separated into two groups of 18 rats each: infected and uninfected. The two groups were divided into three subgroups that received orally distilled water, 30\u00a0mg\/kg ZnO NPs, or 60\u00a0mg\/kg for 10 days. Depending on dosage, ZnO NPs (30, 60\u00a0mg\/kg) drastically reduced larvae in the lung and spleen. ZnO nanoparticles reduced malondialdehyde and nitric oxide and improved the antioxidant system in sick rats\u2019 spleens and lungs. The infected lung and spleen showed fewer histological changes after ZnO NPs (30, 60\u00a0mg\/kg) therapy. Compared to rats infected but untreated, ZnO NPs at 30 and 60\u00a0mg\/kg reduced nuclear factor-kappa B levels in immunohistochemistry. ZnO NPs do not harm animal cells and are harmless to non-infected rats. They can treat\n                    <jats:italic>P. equorum<\/jats:italic>\n                    infection due to their anthelmintic, antioxidant, healing, and anti-inflammatory properties.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-26546-4","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T10:17:01Z","timestamp":1764152221000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Nematocidal activity of zinc oxide nanoparticles synthesized using chicken egg albumin in lung and spleen of Parascaris equorum experimentally infected rats"],"prefix":"10.1038","volume":"15","author":[{"given":"Sara Bayoumi","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman Saber","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohair R.","family":"Fahmy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal","family":"El\u2013Garhy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed R.","family":"Mousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathy","family":"Abdel-Ghaffar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"26546_CR1","doi-asserted-by":"crossref","unstructured":"Morsy, K. et al. 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The protocol used in this study was accepted by the Institutional Animal Care and Use Committee (IACUC) at the Faculty of Science, Cairo University, Egypt (CUI\/F\/85\/20). The experimental procedures followed the international guidelines for the care and use of laboratory animals. Male Wistar rats, ranging in weight from 150 to 160\u00a0g, were purchased from the Dokki branch of the National Research Center (NRC) in Giza. I confirm informed consent was obtained from owners to use the animals in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}}],"article-number":"42518"}}