{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:31:02Z","timestamp":1785364262747,"version":"3.55.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100005324","name":"Universidad Aut\u00f3noma de San Luis Potos\u00ed","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005324","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-28545-x","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T15:59:08Z","timestamp":1767023948000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of a nano antibiotic paste for lesion sterilization and tissue repair technique in pediatric dentistry"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0890-662X","authenticated-orcid":false,"given":"Daniela","family":"Guzm\u00e1n Uribe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4309-2350","authenticated-orcid":false,"given":"Idania","family":"De Alba Montero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4982-5225","authenticated-orcid":false,"given":"Gabriel Alejandro","family":"Mart\u00ednez Casta\u00f1\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6589-5958","authenticated-orcid":false,"given":"Facundo","family":"Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3240-7169","authenticated-orcid":false,"given":"Nereyda","family":"Ni\u00f1o Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"key":"28545_CR1","doi-asserted-by":"publisher","unstructured":"Achanta, A., Reche, A., Dakhale, R. & Bharate, R. R. A comprehensive review of lesion sterilization and tissue repair: an alternative for pulpectomy in deciduous teeth. Cureus https:\/\/doi.org\/10.7759\/cureus.48218 (2023).","DOI":"10.7759\/cureus.48218"},{"key":"28545_CR2","doi-asserted-by":"crossref","unstructured":"Portes Zeno, A. P., Mara\u00f1on-V\u00e1squez, G. A., Guimar\u00e3es Primo, L. & Pintor, B. A. V. & De Castro Costa, M. Pasta CTZ Para abordaje endod\u00f3ncico de Dientes primarios: Una revisi\u00f3n narrativa de La literatura. Rev Odontopediatr\u00eda Latinoam 12, (2022).","DOI":"10.47990\/alop.v12i1.218"},{"key":"28545_CR3","doi-asserted-by":"publisher","first-page":"446","DOI":"10.5005\/jp-journals-10005-1555","volume":"11","author":"S Sain","year":"2018","unstructured":"Sain, S. et al. Lesion sterilization and tissue repair\u2013current concepts and practices. Int. J. Clin. Pediatr. Dent. 11, 446\u2013450 (2018).","journal-title":"Int. J. Clin. Pediatr. Dent."},{"key":"28545_CR4","doi-asserted-by":"publisher","first-page":"369","DOI":"10.17796\/1053-4625-45.6.1","volume":"45","author":"A Garrocho-Rangel","year":"2021","unstructured":"Garrocho-Rangel, A., Jalomo-\u00c1vila, C., Rosales-Berber, M. \u00c1. & Pozos-Guill\u00e9n, A. Lesion sterilization tissue repair (LSTR) approach of non-vital primary molars with a chloramphenicol-tetracycline-ZOE antibiotic paste: A scoping review. J. Clin. Pediatr. Dent. 45, 369\u2013375 (2021).","journal-title":"J. Clin. Pediatr. Dent."},{"key":"28545_CR5","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1002\/cre2.434","volume":"7","author":"OT Sijini","year":"2021","unstructured":"Sijini, O. T. et al. Clinical and radiographic evaluation of triple antibiotic paste pulp therapy compared to Vitapex pulpectomy in non-vital primary molars. Clin. Exp. Dent. Res. 7, 819\u2013828 (2021).","journal-title":"Clin. Exp. Dent. Res."},{"key":"28545_CR6","doi-asserted-by":"crossref","unstructured":"Chouchene, F., Masmoudi, F., Baaziz, A., Maatouk, F. & Ghedira, H. Antibiotic mixtures in noninstrumental endodontic treatment of primary teeth with necrotic pulps: a systematic review. Int. J. Dent. 2021, 1\u201312 (2021).","DOI":"10.1155\/2021\/5518599"},{"key":"28545_CR7","doi-asserted-by":"publisher","first-page":"388","DOI":"10.5005\/jp-journals-10005-1951","volume":"14","author":"S Dhakshinamoorthy","year":"2021","unstructured":"Dhakshinamoorthy, S., Jayakaran, T. G. & Bommareddy, C. S. Comparison of modified triple antibiotic paste in two concentrations for lesion sterilization and tissue repair in primary molars: an in vivo interventional randomized clinical trial. Int. J. Clin. Pediatr. Dent. 14, 388\u2013392 (2021).","journal-title":"Int. J. Clin. Pediatr. Dent."},{"key":"28545_CR8","first-page":"57","volume":"22","author":"K Parakh","year":"2019","unstructured":"Parakh, K. & Shetty, R. M. Evaluation of paste containing gentamicin, amoxicillin and metronidazole in endodontic treatment of primary molars in vivo. Chin. J. Dent. Res. 22, 57\u201364 (2019).","journal-title":"Chin. J. Dent. Res."},{"key":"28545_CR9","doi-asserted-by":"publisher","first-page":"425","DOI":"10.4067\/S0718-381X2016000300008","volume":"10","author":"J Luengo Fereira","year":"2016","unstructured":"Luengo Fereira, J. et al. Efectividad Cl\u00ednica y Radiogr\u00e1fica de La pasta Antibi\u00f3tica CTZ En Pulpotom\u00edas de molares primarios: Ensayo Cl\u00ednico aleatorio controlado. Int. J. Odontostomatol. 10, 425\u2013431 (2016).","journal-title":"Int. J. Odontostomatol"},{"key":"28545_CR10","doi-asserted-by":"publisher","first-page":"e111111637140","DOI":"10.33448\/rsd-v11i16.37140","volume":"11","author":"PS Santos","year":"2022","unstructured":"Santos, P. S. et al. Efficacy of the non-instrumentation endodontic treatment with CTZ paste in primary molars: protocol of a multicenter randomized clinical trial with two years of follow-up. Res. Soc. Dev. 11, e111111637140 (2022).","journal-title":"Res. Soc. Dev."},{"key":"28545_CR11","doi-asserted-by":"publisher","first-page":"e860","DOI":"10.1002\/cre2.860","volume":"10","author":"W Almarji","year":"2024","unstructured":"Almarji, W., Laflouf, M. & Tolibah, Y. A. Evaluation of the modified 3Mix-Simvastatin combination in non\u2010instrumental endodontic therapy of necrotic primary molars: A two\u2010arm randomized controlled trial. Clin. Exp. Dent. Res. 10, e860 (2024).","journal-title":"Clin. Exp. Dent. Res."},{"key":"28545_CR12","unstructured":"L\u00f6vestam, G. et al. Considerations on a Definition of Nanomaterial for Regulatory Purposes (Publications Office, 2010)."},{"key":"28545_CR13","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.3390\/molecules24061033","volume":"24","author":"W Song","year":"2019","unstructured":"Song, W. & Ge, S. Application of antimicrobial nanoparticles in dentistry. Molecules 24, 1033 (2019).","journal-title":"Molecules"},{"key":"28545_CR14","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.3390\/jcm10061152","volume":"10","author":"S Bhandi","year":"2021","unstructured":"Bhandi, S. et al. Antimicrobial efficacy of silver nanoparticles as root canal irrigant\u2019s: A systematic review. J. Clin. Med. 10, 1152 (2021).","journal-title":"J. Clin. Med."},{"key":"28545_CR15","doi-asserted-by":"publisher","first-page":"347","DOI":"10.4103\/ijdr.IJDR_634_15","volume":"29","author":"J De Almeida","year":"2018","unstructured":"De Almeida, J., Cechella, B., Bernardi, A., De Lima Pimenta, A. & Felippe, W. Effectiveness of nanoparticles solutions and conventional endodontic irrigants against Enterococcus faecalis biofilm. Indian J. Dent. Res. 29, 347 (2018).","journal-title":"Indian J. Dent. Res."},{"key":"28545_CR16","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/s40824-020-00198-z","volume":"24","author":"N Raura","year":"2020","unstructured":"Raura, N., Garg, A., Arora, A. & Roma, M. Nanoparticle technology and its implications in endodontics: a review. Biomater. Res. 24, 21 (2020).","journal-title":"Biomater. Res."},{"key":"28545_CR17","doi-asserted-by":"publisher","first-page":"140","DOI":"10.3390\/nano10010140","volume":"10","author":"F Carrouel","year":"2020","unstructured":"Carrouel, F., Viennot, S., Ottolenghi, L., Gaillard, C. & Bourgeois, D. Nanoparticles as anti-microbial, anti-inflammatory, and remineralizing agents in oral care cosmetics: A review of the current situation. Nanomaterials 10, 140 (2020).","journal-title":"Nanomaterials"},{"key":"28545_CR18","doi-asserted-by":"publisher","first-page":"952","DOI":"10.3390\/microorganisms13040952","volume":"13","author":"E Casals","year":"2025","unstructured":"Casals, E., Gusta, M. F., Bastus, N., Rello, J. & Puntes, V. Silver nanoparticles and antibiotics: A promising synergistic approach to multidrug-resistant infections. Microorganisms 13, 952 (2025).","journal-title":"Microorganisms"},{"key":"28545_CR19","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1016\/j.saa.2014.09.120","volume":"136","author":"AL Filgueiras","year":"2015","unstructured":"Filgueiras, A. L., Paschoal, D., Dos Santos, H. F. & Sant\u2019Ana, A. C. Adsorption study of antibiotics on silver nanoparticle surfaces by surface-enhanced Raman scattering spectroscopy. Spectrochim Acta Mol. Biomol. Spectrosc. 136, 979\u2013985 (2015).","journal-title":"Spectrochim Acta Mol. Biomol. Spectrosc."},{"key":"28545_CR20","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1049\/iet-nbt.2015.0005","volume":"10","author":"C Khurana","year":"2016","unstructured":"Khurana, C., Vala, A. K., Andhariya, N., Pandey, O. P. & Chudasama, B. Influence of antibiotic adsorption on biocidal activities of silver nanoparticles. IET Nanobiotechnol. 10, 69\u201374 (2016).","journal-title":"IET Nanobiotechnol."},{"key":"28545_CR21","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1590\/0103-6440202003897","volume":"31","author":"JFB Bruniera","year":"2020","unstructured":"Bruniera, J. F. B. et al. Green synthesis, characterization and antimicrobial evaluation of silver nanoparticles for an intracanal dressing. Braz Dent. J. 31, 485\u2013492 (2020).","journal-title":"Braz Dent. J."},{"key":"28545_CR22","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1007\/s11051-008-9428-6","volume":"10","author":"GA Mart\u00ednez-Casta\u00f1on","year":"2008","unstructured":"Mart\u00ednez-Casta\u00f1on, G. A., Nino, N., Mart\u00ednez-Guti\u00e9rrez, F., Martinez, J. R. & Ruiz, F. Synthesis and antibacterial activity of silver nanoparticles with different sizes. J. Nanoparticle Res. 10, 1343\u20131348 (2008).","journal-title":"J. Nanoparticle Res."},{"key":"28545_CR23","first-page":"273","volume":"19","author":"K Ranoszek-Soliwoda","year":"2017","unstructured":"Ranoszek-Soliwoda, K. et al. The role of tannic acid and sodium citrate in the synthesis of silver nanoparticles. J. Nanoparticle Res. Interdiscip Forum Nanoscale Sci. Technol. 19, 273 (2017).","journal-title":"J. Nanoparticle Res. Interdiscip Forum Nanoscale Sci. Technol."},{"key":"28545_CR24","unstructured":"M100. Performance Standards for Antimicrobial Susceptibility Testing."},{"key":"28545_CR25","unstructured":"R Core Team. R: A Language and Environment for Statistical Computing (R Foundation for Statistical Computing, 2024)."},{"key":"28545_CR26","doi-asserted-by":"publisher","first-page":"361","DOI":"10.4103\/ccd.ccd_243_18","volume":"9","author":"B Chakraborty","year":"2018","unstructured":"Chakraborty, B., Nayak, A. & Rao, A. Efficacy of lesion sterilization and tissue repair in primary tooth with internal resorption: A case series. Contemp. Clin. Dent. 9, 361 (2018).","journal-title":"Contemp. Clin. Dent."},{"key":"28545_CR27","doi-asserted-by":"publisher","first-page":"e0291133","DOI":"10.1371\/journal.pone.0291133","volume":"18","author":"APT Sobral","year":"2023","unstructured":"Sobral, A. P. T. et al. Efficacy of antibiotic and iodoform pastes in non-instrumental endodontic treatment of anterior primary teeth\u2014Protocol for a randomized controlled clinical. PLOS ONE. 18, e0291133 (2023).","journal-title":"PLOS ONE"},{"key":"28545_CR28","first-page":"110","volume":"47","author":"G Pardi","year":"2009","unstructured":"Pardi, G., Guilarte, C., Cardozo, E. I. & Brice\u00f1o, E. N. Detecci\u00f3n de Enterococcus faecalis En Dientes Con Fracaso En El Tratamiento Endod\u00f3ntico. Acta Odontol\u00f3gica Venez. 47, 110\u2013121 (2009).","journal-title":"Acta Odontol\u00f3gica Venez."},{"key":"28545_CR29","unstructured":"Yelle, S. & Mascarenhas, J. Detection of ESBL Producing Gram-Negative Bacteria in Public Drinking Water Sources from South Mumbai. 288\u2013300 (2020)."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-28545-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-28545-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-28545-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T12:23:17Z","timestamp":1777119797000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-28545-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,29]]},"references-count":29,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["28545"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-28545-x","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,29]]},"assertion":[{"value":"13 August 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"44880"}}