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Knowledge about its causes helps the clinician in proper instrument and technique selection to decrease its incidence. Therefore, the aim of this randomized clinical trial was to compare the post-operative pain occurrence after single visit root canal preparation using ProTaper Universal rotary system or M-Pro rotary system. Eighty patients with symptomatic irreversible pulpitis in mandibular first molars were allocated into two groups. In group A (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200940): root canal preparation was performed using ProTaper Universal system and in group B (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200940): root canal preparation was performed using M-Pro rotary system. Pain level was assessed by the patient using the numerical rating scale (NRS) at 24\u00a0h and 7 days postoperatively. The patients were advised to take the prescribed analgesics in case of emergency need. Data and statistical analysis showed that there was significant decrease in pain in both groups after 24\u00a0h and after 7 days. Insignificant difference was found between ProTaper Universal group and M-Pro group after 24\u00a0h and after 7 days. Insignificant difference was found in analgesics intake between both groups after 24\u00a0h and after 7 days.\n                  <\/jats:p>\n                  <jats:p>\n                    Trial registration: The trial protocol was registered at\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/ClinicalTrials.gov\" ext-link-type=\"uri\">https:\/\/ClinicalTrials.gov<\/jats:ext-link>\n                    (NCT06777381), registered January 15, 2024.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-29029-8","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T17:16:57Z","timestamp":1766078217000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of post-operative pain prevalence after single visit endodontic treatment with two NiTi rotary files - a randomized clinical trial"],"prefix":"10.1038","volume":"15","author":[{"given":"M. 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