{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T16:03:46Z","timestamp":1781625826267,"version":"3.54.5"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T00:00:00Z","timestamp":1765065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":29,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Suzhou Health Commission, Jiangsu Province","award":["Szlcyxzx202108"],"award-info":[{"award-number":["Szlcyxzx202108"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), is a serious infectious disease. Previous studies have shown that endoplasmic reticulum (ER) stress plays an important role in various infectious processes. This study aimed to identify potential ER stress-related genes in TB by analyzing differentially expressed genes (DEGs) and to explore the role of ER stress in MTB infection. Ten ER stress-related DEGs (ERSRDEGs) were identified by standardizing and analyzing differential expression in the dataset GSE114911. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that these ERSRDEGs are significantly involved in neutrophil migration and the\n                    <jats:italic>IL-17<\/jats:italic>\n                    signaling pathway. A protein-protein interaction network identified four hub genes (\n                    <jats:italic>IL-1B<\/jats:italic>\n                    ,\n                    <jats:italic>CCL20<\/jats:italic>\n                    ,\n                    <jats:italic>IL -1\u00a0A<\/jats:italic>\n                    ,\n                    <jats:italic>and TNF<\/jats:italic>\n                    ), among which gene\n                    <jats:italic>IL-1B<\/jats:italic>\n                    showed highly significant differential expression in the independent dataset GSE147964, demonstrating excellent diagnostic performance (AUC\u2009=\u20090.93). This finding was further validated by Enzyme-linked immunosorbent assay (ELISA), which confirmed high expression of\n                    <jats:italic>IL-1B<\/jats:italic>\n                    in the plasma of TB patients. Immune infiltration analysis showed increased infiltration of M1 macrophages in the TB infection group, with gene\n                    <jats:italic>IL-1B<\/jats:italic>\n                    strongly positively correlated with M1 macrophage abundance. Additionally, the study analyzed correlations between genes\n                    <jats:italic>IL-1\u00a0A<\/jats:italic>\n                    and\n                    <jats:italic>IL-1B<\/jats:italic>\n                    and clinical indicators, including inflammatory factors and D-dimer levels. The results indicated that gene\n                    <jats:italic>IL-1B<\/jats:italic>\n                    was positively correlated with\n                    <jats:italic>IL-6<\/jats:italic>\n                    ,\n                    <jats:italic>TNF-\u03b1<\/jats:italic>\n                    , and\n                    <jats:italic>IFN-\u03b3<\/jats:italic>\n                    , while gene\n                    <jats:italic>IL-1\u00a0A<\/jats:italic>\n                    was positively correlated with D-dimer. Overall, these findings emphasize the critical role of ER stress-related genes in the pathophysiology of MTB infection.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-29599-7","type":"journal-article","created":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T18:13:53Z","timestamp":1765131233000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification and clinical validation of endoplasmic reticulum genes related to pulmonary tuberculosis"],"prefix":"10.1038","volume":"16","author":[{"given":"Min","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiying","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-feng","family":"Min","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,7]]},"reference":[{"key":"29599_CR1","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1007\/s10096-023-04613-w","volume":"42","author":"VAC Castro-Lima","year":"2023","unstructured":"Castro-Lima, V. A. C. et al. Prevalence of latent Mycobacterium tuberculosis infection in hematopoietic stem cell transplantation comparing tuberculin skin test and interferon-gamma release assay. Eur. J. Clin. Microbiol. Infect. Diseases: Official Publication Eur. Soc. Clin. Microbiol. 42, 899\u2013902. https:\/\/doi.org\/10.1007\/s10096-023-04613-w (2023).","journal-title":"Eur. J. Clin. Microbiol. Infect. Diseases: Official Publication Eur. Soc. Clin. Microbiol."},{"key":"29599_CR2","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1146\/annurev-pathol-012513-104649","volume":"10","author":"SA Oakes","year":"2015","unstructured":"Oakes, S. A. & Papa, F. R. The role of Endoplasmic reticulum stress in human pathology. Annu. Rev. Pathol. 10, 173\u2013194. https:\/\/doi.org\/10.1146\/annurev-pathol-012513-104649 (2015).","journal-title":"Annu. Rev. Pathol."},{"key":"29599_CR3","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1007\/978-1-4614-1680-7_10","volume":"738","author":"VM Parmar","year":"2012","unstructured":"Parmar, V. M. & Schr\u00f6der, M. Sensing Endoplasmic reticulum stress. Adv. Exp. Med. Biol. 738, 153\u2013168. https:\/\/doi.org\/10.1007\/978-1-4614-1680-7_10 (2012).","journal-title":"Adv. Exp. Med. Biol."},{"key":"29599_CR4","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1089\/dna.2022.0353","volume":"41","author":"Y Tang","year":"2022","unstructured":"Tang, Y. et al. Endoplasmic reticulum stress and oxidative stress in inflammatory diseases. DNA Cell Biol. 41, 924\u2013934. https:\/\/doi.org\/10.1089\/dna.2022.0353 (2022).","journal-title":"DNA Cell Biol."},{"key":"29599_CR5","doi-asserted-by":"publisher","first-page":"e12772","DOI":"10.1371\/journal.pone.0012772","volume":"5","author":"TA Seimon","year":"2010","unstructured":"Seimon, T. A. et al. Induction of ER stress in macrophages of tuberculosis granulomas. PloS One. 5, e12772. https:\/\/doi.org\/10.1371\/journal.pone.0012772 (2010).","journal-title":"PloS One"},{"key":"29599_CR6","doi-asserted-by":"publisher","unstructured":"Gebremariam, G. et al. Trend of pulmonary tuberculosis and rifampicin-resistance among tuberculosis presumptive patients in central Tigray, Ethiopia; 2018\u20132023: a six-year retrospective study. Trop. Dis. Travel Med. Vaccines. 10 (15). https:\/\/doi.org\/10.1186\/s40794-024-00224-1 (2024).","DOI":"10.1186\/s40794-024-00224-1"},{"key":"29599_CR7","doi-asserted-by":"publisher","first-page":"105147","DOI":"10.1016\/j.micpath.2021.105147","volume":"159","author":"MA Abo-Kadoum","year":"2021","unstructured":"Abo-Kadoum, M. A. et al. Mycobacterium tuberculosis PE17 (Rv1646) promotes host cell apoptosis via host chromatin remodeling mediated by reduced H3K9me3 occupancy. Microb. Pathog. 159, 105147. https:\/\/doi.org\/10.1016\/j.micpath.2021.105147 (2021).","journal-title":"Microb. Pathog."},{"key":"29599_CR8","doi-asserted-by":"publisher","unstructured":"Maertzdorf, J. et al. Mycobacterium tuberculosis invasion of the human lung: first contact. Front. Immunol. 9 https:\/\/doi.org\/10.3389\/\u200bf\u200bimmu.2018.01346 (2018).","DOI":"10.3389\/\u200bf\u200bimmu.2018.01346"},{"key":"29599_CR9","doi-asserted-by":"publisher","unstructured":"Barrett, T. et al. NCBI GEO: archive for functional genomics data sets\u2013update. Nucleic Acids Res. 41 (D991-995). https:\/\/doi.org\/10.1093\/nar\/gks1193 (2013).","DOI":"10.1093\/nar\/gks1193"},{"key":"29599_CR10","doi-asserted-by":"publisher","unstructured":"Stelzer, G. et al. The genecards suite: from gene data mining to disease genome sequence analyses. Curr. Protocols Bioinf. 54 https:\/\/doi.org\/10.1002\/cpbi.5 (2016). 1.30.31\u201331.30.33.","DOI":"10.1002\/cpbi.5"},{"key":"29599_CR11","doi-asserted-by":"publisher","first-page":"1739","DOI":"10.1093\/bioinformatics\/btr260","volume":"27","author":"A Liberzon","year":"2011","unstructured":"Liberzon, A. et al. Molecular signatures database (MSigDB) 3.0. Bioinf. (Oxford England). 27, 1739\u20131740. https:\/\/doi.org\/10.1093\/bioinformatics\/btr260 (2011).","journal-title":"Bioinf. (Oxford England)"},{"key":"29599_CR12","doi-asserted-by":"publisher","unstructured":"Xu, X., Tang, Y., Zhu, J. & Luo, J. Endoplasmic reticulum Stress-Related Four-Biomarker risk classifier for survival evaluation in esophageal cancer. J. Oncol. 2022 (5860671). https:\/\/doi.org\/10.1155\/2022\/5860671 (2022).","DOI":"10.1155\/2022\/5860671"},{"key":"29599_CR13","doi-asserted-by":"publisher","first-page":"928006","DOI":"10.3389\/fmolb.2022.928006","volume":"9","author":"Y Shen","year":"2022","unstructured":"Shen, Y., Cao, Y., Zhou, L., Wu, J. & Mao, M. Construction of an Endoplasmic reticulum stress-related gene model for predicting prognosis and immune features in kidney renal clear cell carcinoma. Front. Mol. Biosci. 9, 928006. https:\/\/doi.org\/10.3389\/fmolb.2022.928006 (2022).","journal-title":"Front. Mol. Biosci."},{"key":"29599_CR14","doi-asserted-by":"publisher","first-page":"975774","DOI":"10.3389\/fphar.2022.975774","volume":"13","author":"Y Lai","year":"2022","unstructured":"Lai, Y. et al. Identification of Endoplasmic reticulum stress-associated genes and subtypes for prediction of alzheimer\u2019s disease based on interpretable machine learning. Front. Pharmacol. 13, 975774. https:\/\/doi.org\/10.3389\/fphar.2022.975774 (2022).","journal-title":"Front. Pharmacol."},{"key":"29599_CR15","doi-asserted-by":"publisher","unstructured":"Ritchie, M. E. et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic acids research 43, e47, (2015). https:\/\/doi.org\/10.1093\/nar\/gkv007","DOI":"10.1093\/nar\/gkv007"},{"key":"29599_CR16","first-page":"383","volume":"99","author":"K Ben Salem","year":"2021","unstructured":"Ben Salem, K. Ben Abdelaziz, A. Principal component analysis (PCA). Tunis. Med. 99, 383\u2013389 (2021).","journal-title":"Tunis. Med."},{"key":"29599_CR17","doi-asserted-by":"publisher","unstructured":"Zhang, H., Meltzer, P. & Davis, S. RCircos: an R package for circos 2D track plots. BMC Bioinform. 14 https:\/\/doi.org\/10.1186\/1471-2105-14-244 (2013).","DOI":"10.1186\/1471-2105-14-244"},{"key":"29599_CR18","doi-asserted-by":"publisher","first-page":"D419","DOI":"10.1093\/nar\/gky1038","volume":"47","author":"H Mi","year":"2019","unstructured":"Mi, H., Muruganujan, A., Ebert, D., Huang, X. & Thomas, P. D. PANTHER version 14: more genomes, a new PANTHER GO-slim and improvements in enrichment analysis tools. Nucleic Acids Res. 47, D419\u2013d426. https:\/\/doi.org\/10.1093\/nar\/gky1038 (2019).","journal-title":"Nucleic Acids Res."},{"key":"29599_CR19","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1093\/nar\/28.1.27","volume":"28","author":"M Kanehisa","year":"2000","unstructured":"Kanehisa, M. & Goto, S. KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28, 27\u201330. https:\/\/doi.org\/10.1093\/nar\/28.1.27 (2000).","journal-title":"Nucleic Acids Res."},{"key":"29599_CR20","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1089\/omi.2011.0118","volume":"16","author":"G Yu","year":"2012","unstructured":"Yu, G., Wang, L. G., Han, Y. & He, Q. Y. ClusterProfiler: an R package for comparing biological themes among gene clusters. Omics: J. Integr. Biology. 16, 284\u2013287. https:\/\/doi.org\/10.1089\/omi.2011.0118 (2012).","journal-title":"Omics: J. Integr. Biology"},{"key":"29599_CR21","doi-asserted-by":"publisher","first-page":"15545","DOI":"10.1073\/pnas.0506580102","volume":"102","author":"A Subramanian","year":"2005","unstructured":"Subramanian, A. et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc. Natl. Acad. Sci. U.S.A. 102, 15545\u201315550. https:\/\/doi.org\/10.1073\/pnas.0506580102 (2005).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"29599_CR22","doi-asserted-by":"publisher","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","volume":"51","author":"D Szklarczyk","year":"2023","unstructured":"Szklarczyk, D. et al. The STRING database in 2023: protein-protein association networks and functional enrichment analyses for any sequenced genome of interest. Nucleic Acids Res. 51, D638\u2013d646. https:\/\/doi.org\/10.1093\/nar\/gkac1000 (2023).","journal-title":"Nucleic Acids Res."},{"key":"29599_CR23","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","volume":"13","author":"P Shannon","year":"2003","unstructured":"Shannon, P. et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 13, 2498\u20132504. https:\/\/doi.org\/10.1101\/gr.1239303 (2003).","journal-title":"Genome Res."},{"key":"29599_CR24","doi-asserted-by":"publisher","unstructured":"Chin, C. H. et al. CytoHubba: identifying hub objects and sub-networks from complex interactome. BMC Syst. Biol. 8 (Suppl 4). https:\/\/doi.org\/10.1186\/1752-0509-8-s4-s11 (2014).","DOI":"10.1186\/1752-0509-8-s4-s11"},{"key":"29599_CR25","doi-asserted-by":"publisher","first-page":"W214","DOI":"10.1093\/nar\/gkq537","volume":"38","author":"D Warde-Farley","year":"2010","unstructured":"Warde-Farley, D. et al. The genemania prediction server: biological network integration for gene prioritization and predicting gene function. Nucleic Acids Res. 38, W214\u2013220. https:\/\/doi.org\/10.1093\/nar\/gkq537 (2010).","journal-title":"Nucleic Acids Res."},{"key":"29599_CR26","doi-asserted-by":"publisher","first-page":"D43","DOI":"10.1093\/nar\/gkw965","volume":"45","author":"KR Zhou","year":"2017","unstructured":"Zhou, K. R. et al. ChIPBase v2.0: decoding transcriptional regulatory networks of non-coding RNAs and protein-coding genes from ChIP-seq data. Nucleic Acids Res. 45, D43\u2013d50. https:\/\/doi.org\/10.1093\/nar\/gkw965 (2017).","journal-title":"Nucleic Acids Res."},{"key":"29599_CR27","doi-asserted-by":"publisher","first-page":"D92","DOI":"10.1093\/nar\/gkt1248","volume":"42","author":"JH Li","year":"2014","unstructured":"Li, J. H., Liu, S., Zhou, H., Qu, L. H. & Yang, J. H. StarBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data. Nucleic Acids Res. 42, D92\u201397. https:\/\/doi.org\/10.1093\/nar\/gkt1248 (2014).","journal-title":"Nucleic Acids Res."},{"key":"29599_CR28","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/1471-2105-12-77","volume":"12","author":"X Robin","year":"2011","unstructured":"Robin, X. et al. pROC: an open-source package for R and S\u2009+\u2009to analyze and compare ROC curves. BMC Bioinform. 12, 77. https:\/\/doi.org\/10.1186\/1471-2105-12-77 (2011).","journal-title":"BMC Bioinform."},{"key":"29599_CR29","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1038\/nmeth.3337","volume":"12","author":"AM Newman","year":"2015","unstructured":"Newman, A. M. et al. Robust enumeration of cell subsets from tissue expression profiles. Nat. Methods. 12, 453\u2013457. https:\/\/doi.org\/10.1038\/nmeth.3337 (2015).","journal-title":"Nat. Methods"},{"key":"29599_CR30","doi-asserted-by":"publisher","first-page":"01","DOI":"10.7860\/jcdr\/2016\/19052.8286","volume":"10","author":"S Yadav","year":"2016","unstructured":"Yadav, S., Rawal, G., Baxi, M. & Bedaquiline A novel antitubercular agent for the treatment of Multidrug-Resistant tuberculosis. J. Clin. Diagn. Research: JCDR. 10, 01\u201302. https:\/\/doi.org\/10.7860\/jcdr\/2016\/19052.8286 (2016).","journal-title":"J. Clin. Diagn. Research: JCDR"},{"key":"29599_CR31","doi-asserted-by":"publisher","first-page":"471","DOI":"10.2471\/blt.18.208959","volume":"96","author":"A Richterman","year":"2018","unstructured":"Richterman, A. et al. Cash interventions to improve clinical outcomes for pulmonary tuberculosis: systematic review and meta-analysis. Bull. World Health Organ. 96, 471\u2013483. https:\/\/doi.org\/10.2471\/blt.18.208959 (2018).","journal-title":"Bull. World Health Organ."},{"key":"29599_CR32","doi-asserted-by":"publisher","unstructured":"Dheda, K. et al. Multidrug-resistant tuberculosis. Nat. Reviews Disease Primers. 10 https:\/\/doi.org\/10.1038\/s41572-024-00504-2 (2024).","DOI":"10.1038\/s41572-024-00504-2"},{"key":"29599_CR33","doi-asserted-by":"publisher","first-page":"109249","DOI":"10.1016\/j.biopha.2019.109249","volume":"118","author":"Y Lin","year":"2019","unstructured":"Lin, Y., Jiang, M., Chen, W., Zhao, T. & Wei, Y. Cancer and ER stress: mutual crosstalk between autophagy, oxidative stress and inflammatory response. Biomed. pharmacotherapy = Biomedecine Pharmacotherapie. 118, 109249. https:\/\/doi.org\/10.1016\/j.biopha.2019.109249 (2019).","journal-title":"Biomed. pharmacotherapy = Biomedecine Pharmacotherapie"},{"key":"29599_CR34","doi-asserted-by":"publisher","first-page":"111058","DOI":"10.1016\/j.intimp.2023.111058","volume":"124","author":"J Chen","year":"2023","unstructured":"Chen, J. et al. Mycobacterium tuberculosis suppresses APLP2 expression to enhance its survival in macrophage. Int. Immunopharmacol. 124, 111058. https:\/\/doi.org\/10.1016\/j.intimp.2023.111058 (2023).","journal-title":"Int. Immunopharmacol."},{"key":"29599_CR35","doi-asserted-by":"publisher","unstructured":"Alcantara, C. A., Glassman, I., Nguyen, K. H. & Parthasarathy, A. Venketaraman, V. Neutrophils in Mycobacterium tuberculosis. Vaccines 11 https:\/\/doi.org\/10.3390\/vaccines11030631 (2023).","DOI":"10.3390\/vaccines11030631"},{"key":"29599_CR36","doi-asserted-by":"publisher","first-page":"1255905","DOI":"10.3389\/fcimb.2023.1255905","volume":"13","author":"J Wei","year":"2023","unstructured":"Wei, J. et al. Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen. Front. Cell. Infect. Microbiol. 13, 1255905. https:\/\/doi.org\/10.3389\/fcimb.2023.1255905 (2023).","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"29599_CR37","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1111\/jdv.16866","volume":"35","author":"M Nogueira","year":"2021","unstructured":"Nogueira, M., Warren, R. B. & Torres, T. Risk of tuberculosis reactivation with Interleukin (IL)-17 and IL-23 inhibitors in psoriasis - time for a paradigm change. J. Eur. Acad. Dermatology Venereology: JEADV. 35, 824\u2013834. https:\/\/doi.org\/10.1111\/jdv.16866 (2021).","journal-title":"J. Eur. Acad. Dermatology Venereology: JEADV"},{"key":"29599_CR38","doi-asserted-by":"publisher","unstructured":"Abhimanyu, Bose, M., Giri, A. & Varma-Basil, M. Comparative genetic association analysis of human genetic susceptibility to pulmonary and lymph node tuberculosis. Genes 14 https:\/\/doi.org\/10.3390\/genes14010207 (2023).","DOI":"10.3390\/genes14010207"},{"key":"29599_CR39","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1093\/cid\/ciab729","volume":"74","author":"C Nwongbouwoh Muefong","year":"2022","unstructured":"Nwongbouwoh Muefong, C. et al. Neutrophils contribute to severity of tuberculosis pathology and recovery from lung damage Pre- and posttreatment. Clin. Infect. Diseases: Official Publication Infect. Dis. Soc. Am. 74, 1757\u20131766. https:\/\/doi.org\/10.1093\/cid\/ciab729 (2022).","journal-title":"Clin. Infect. Diseases: Official Publication Infect. Dis. Soc. Am."},{"key":"29599_CR40","doi-asserted-by":"publisher","first-page":"2239940","DOI":"10.1080\/22221751.2023.2239940","volume":"12","author":"Q Yang","year":"2023","unstructured":"Yang, Q. et al. The interaction of macrophages and CD8 T cells in Bronchoalveolar lavage fluid is associated with latent tuberculosis infection. Emerg. Microbes Infections. 12, 2239940. https:\/\/doi.org\/10.1080\/22221751.2023.2239940 (2023).","journal-title":"Emerg. Microbes Infections"},{"key":"29599_CR41","doi-asserted-by":"publisher","first-page":"10799","DOI":"10.1080\/21655979.2021.2003931","volume":"12","author":"Y Li","year":"2021","unstructured":"Li, Y., Deng, Y. & He, J. Monocyte-related gene biomarkers for latent and active tuberculosis. Bioengineered 12, 10799\u201310811. https:\/\/doi.org\/10.1080\/21655979.2021.2003931 (2021).","journal-title":"Bioengineered"},{"key":"29599_CR42","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/s11882-024-01128-y","volume":"24","author":"D Voelker","year":"2024","unstructured":"Voelker, D. & Pongdee, T. Urine mast cell mediators in the evaluation and diagnosis of mast cell activation syndrome. Curr. Allergy Asthma Rep. 24, 33\u201338. https:\/\/doi.org\/10.1007\/s11882-024-01128-y (2024).","journal-title":"Curr. Allergy Asthma Rep."},{"key":"29599_CR43","doi-asserted-by":"publisher","first-page":"9253","DOI":"10.1038\/s41598-021-88545-5","volume":"11","author":"M Mburu","year":"2021","unstructured":"Mburu, M., Paquet-Durand, O., Hitzmann, B. & Zettel, V. Spectroscopic analysis of Chia seeds. Sci. Rep. 11, 9253. https:\/\/doi.org\/10.1038\/s41598-021-88545-5 (2021).","journal-title":"Sci. Rep."},{"key":"29599_CR44","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1038\/s41598-023-28135-9","volume":"13","author":"\u00d8 Midttun","year":"2023","unstructured":"Midttun, \u00d8. et al. A cross-sectional study of inflammatory markers as determinants of Circulating kynurenines in the lung cancer cohort consortium. Sci. Rep. 13, 1011. https:\/\/doi.org\/10.1038\/s41598-023-28135-9 (2023).","journal-title":"Sci. Rep."},{"key":"29599_CR45","doi-asserted-by":"publisher","first-page":"152346","DOI":"10.1016\/j.imbio.2023.152346","volume":"228","author":"CD S\u00e1nchez-Barinas","year":"2023","unstructured":"S\u00e1nchez-Barinas, C. D. et al. Peptide-pulsed dendritic cells\u2019 Immunomodulating effect regarding Mycobacterium tuberculosis growth in macrophages. Immunobiology 228, 152346. https:\/\/doi.org\/10.1016\/j.imbio.2023.152346 (2023).","journal-title":"Immunobiology"},{"key":"29599_CR46","doi-asserted-by":"publisher","unstructured":"Zhang, J. et al. Lead disrupts mitochondrial morphology and function through induction of ER stress in model of neurotoxicity. Int. J. Mol. Sci. 23 https:\/\/doi.org\/10.3390\/ijms231911435 (2022).","DOI":"10.3390\/ijms231911435"},{"key":"29599_CR47","doi-asserted-by":"publisher","first-page":"2204","DOI":"10.1021\/acsomega.3c05589","volume":"9","author":"S Harakeh","year":"2024","unstructured":"Harakeh, S. et al. Integrated network Pharmacology approach to evaluate bioactive phytochemicals of acalypha indica and their mechanistic actions to suppress target genes of tuberculosis. ACS Omega. 9, 2204\u20132219. https:\/\/doi.org\/10.1021\/acsomega.3c05589 (2024).","journal-title":"ACS Omega"},{"key":"29599_CR48","doi-asserted-by":"publisher","first-page":"19509","DOI":"10.1038\/s41598-020-72514-5","volume":"10","author":"R Xiang","year":"2020","unstructured":"Xiang, R. et al. Framework, method and case study for the calculation of end of life for HWL and parameter sensitivity analysis. Sci. Rep. 10, 19509. https:\/\/doi.org\/10.1038\/s41598-020-72514-5 (2020).","journal-title":"Sci. Rep."},{"key":"29599_CR49","doi-asserted-by":"publisher","first-page":"109303","DOI":"10.1016\/j.compbiomed.2024.109303","volume":"183","author":"S Park","year":"2024","unstructured":"Park, S. et al. Plasma protein-based identification of neuroimage-driven subtypes in mild cognitive impairment via protein-protein interaction aware explainable graph propagational network. Comput. Biol. Med. 183, 109303. https:\/\/doi.org\/10.1016\/j.compbiomed.2024.109303 (2024).","journal-title":"Comput. Biol. Med."},{"key":"29599_CR50","doi-asserted-by":"publisher","first-page":"1317","DOI":"10.3855\/jidc.16721","volume":"16","author":"I Buha","year":"2022","unstructured":"Buha, I. et al. Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia. J. Infect. Developing Ctries. 16, 1317\u20131322. https:\/\/doi.org\/10.3855\/jidc.16721 (2022).","journal-title":"J. Infect. Developing Ctries."},{"key":"29599_CR51","doi-asserted-by":"publisher","first-page":"e27869","DOI":"10.1097\/md.0000000000027869","volume":"100","author":"X Li","year":"2021","unstructured":"Li, X., He, J., Wang, G. & Sun, J. Diagnostic value of microRNA-155 in active tuberculosis: A systematic review and meta-analysis. Medicine 100, e27869. https:\/\/doi.org\/10.1097\/md.0000000000027869 (2021).","journal-title":"Medicine"},{"key":"29599_CR52","doi-asserted-by":"publisher","unstructured":"Zheng, J. et al. Expression and clinical significance of LncRNA NEAT1 in patients with spinal tuberculosis. Dis. Markers. 2022 (5748756). https:\/\/doi.org\/10.1155\/2022\/5748756 (2022).","DOI":"10.1155\/2022\/5748756"},{"key":"29599_CR53","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1007\/s13353-023-00808-1","volume":"65","author":"FM Aldakheel","year":"2024","unstructured":"Aldakheel, F. M., Syed, R., Ahmed, M. & Xu, T. Modulation of LncRNA NEAT1 overturns the macrophages based immune response in M. tuberculosis infected patients via miR-373 regulation. J. Appl. Genet. 65, 321\u2013329. https:\/\/doi.org\/10.1007\/s13353-023-00808-1 (2024).","journal-title":"J. Appl. Genet."},{"key":"29599_CR54","doi-asserted-by":"publisher","unstructured":"Poladian, N. et al. Role of NF-\u03baB during Mycobacterium tuberculosis infection. Int. J. Mol. Sci. 24 https:\/\/doi.org\/10.3390\/ijms24021772 (2023).","DOI":"10.3390\/ijms24021772"},{"key":"29599_CR55","doi-asserted-by":"publisher","unstructured":"Ma, R. et al. Single-cell transcriptome analysis reveals the dysregulated monocyte state associated with tuberculosis progression. BMC Infect. Dis. 25 https:\/\/doi.org\/10.1186\/s12879-025-10612-3 (2025).","DOI":"10.1186\/s12879-025-10612-3"},{"key":"29599_CR56","doi-asserted-by":"publisher","first-page":"3847","DOI":"10.2147\/idr.S412116","volume":"16","author":"J Liu","year":"2023","unstructured":"Liu, J. et al. Novel biomarker panel of Let-7d-5p and MiR-140-5p can distinguish latent tuberculosis infection from active tuberculosis patients. Infect. Drug Resist. 16, 3847\u20133859. https:\/\/doi.org\/10.2147\/idr.S412116 (2023).","journal-title":"Infect. Drug Resist."},{"key":"29599_CR57","doi-asserted-by":"publisher","unstructured":"Wu, X. et al. Integrated bioinformatics analysis of dendritic cells hub genes reveal potential early tuberculosis diagnostic markers. BMC Med. Genom. 16 https:\/\/doi.org\/10.1186\/s12920-023-01646-0 (2023).","DOI":"10.1186\/s12920-023-01646-0"},{"key":"29599_CR58","doi-asserted-by":"publisher","first-page":"e918906","DOI":"10.12659\/msm.918906","volume":"26","author":"X Liu","year":"2020","unstructured":"Liu, X. et al. Integrative gene expression profiling analysis to investigate potential prognostic biomarkers for colorectal cancer. Med. Sci. Monitor: Int. Med. J. Experimental Clin. Res. 26, e918906. https:\/\/doi.org\/10.12659\/msm.918906 (2020).","journal-title":"Med. Sci. Monitor: Int. Med. J. Experimental Clin. Res."},{"key":"29599_CR59","doi-asserted-by":"publisher","first-page":"135","DOI":"10.7754\/Clin.Lab.2017.170720","volume":"64","author":"W Min","year":"2018","unstructured":"Min, W., Zi-Feng, J., Jian-Lin, X. & Hao-Hui, F. Role of the fibrinogen degradation products and D-Dimer in the differential diagnosis of pulmonary tuberculosis and Community-Acquired pneumonia. Clin. Lab. 64, 135\u2013140. https:\/\/doi.org\/10.7754\/Clin.Lab.2017.170720 (2018).","journal-title":"Clin. Lab."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-29599-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-29599-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-29599-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:07:44Z","timestamp":1781622464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-29599-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,7]]},"references-count":59,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["29599"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-29599-7","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-6582977\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,7]]},"assertion":[{"value":"3 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The clinical study, which included blood samples and information of participants, was reviewed and approved by the Ethics Committee of Fifth People\u2019s Hospital of Suzhou, Department of Pulmonology, Suzhou, Jiangsu, People\u2019s Republic of China (Approval No: ZF-2024-008-01) .All procedures were carried out in accordance with the Declaration of Helsinki. Written informed consent was obtained from all participants prior to study procedures.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and informed consent"}}],"article-number":"228"}}