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This study aimed to investigate the cytotoxic potential of flavonoids isolated from\n                    <jats:italic>Erythrina crista-galli<\/jats:italic>\n                    in MCF-7 breast cancer cells using in vitro and in silico analyses. Three flavonoids were isolated from the twigs of\n                    <jats:italic>E. crista-galli<\/jats:italic>\n                    using multiple column chromatography. Cytotoxic activity was evaluated against the MCF-7 breast cancer cell line using the MTT assay, whereas the in silico study was evaluated using molecular docking and molecular dynamics against the epidermal growth factor receptor (EGFR). Three flavonoids, naringenin (1), daidzein (2), and isoliquiritigenin (3), were isolated from the ethanol extract of\n                    <jats:italic>E. crista-galli<\/jats:italic>\n                    twigs. Their structures were confirmed using multiple spectroscopic methods. In vitro cytotoxicity testing against MCF-7 breast cancer cells showed that naringenin (1) and isoliquiritigenin (3) exhibited moderate activity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 105.60 and 84.21\u00a0\u00b5g\/mL, respectively, whereas daidzein (2) displayed weak activity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    of 239.77\u00a0\u00b5g\/mL. Network pharmacology analysis identified multiple targets of isoliquiritigenin related to breast cancer, with epidermal growth factor receptor (EGFR) as one of the key hub.\u00a0Molecular docking indicated a stronger binding affinity of isoliquiritigenin compared to ATP, while molecular dynamics simulations confirmed the stability of the EGFR\u2013isoliquiritigenin complex with binding free energy estimated at \u2212\u200930.002\u2009\u00b1\u20093.879\u00a0kcal\/mol. Collectively, these results highlight isoliquiritigenin as a promising EGFR-targeted cytotoxic compound and\n                    <jats:italic>E. crista-galli<\/jats:italic>\n                    as a valuable source of bioactive flavonoids for anticancer drug development, while emphasizing the need for further in vivo validation and toxicity assessment.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-32400-4","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T16:25:54Z","timestamp":1766075154000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In vitro and in silico evaluation of flavonoids from Erythrina crista-galli with cytotoxic potential against MCF-7 breast cancer cell"],"prefix":"10.1038","volume":"16","author":[{"given":"Tati","family":"Herlina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melati","family":"Sukma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abd. 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