{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:53:51Z","timestamp":1785164031772,"version":"3.55.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":21,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Accurate biodosimetry is critical for assessing radiation exposure in radiological emergencies, occupational monitoring, and clinical management, where precise dose estimation informs life-saving decisions and regulatory compliance. Current gold-standard cytogenetic methods face limitations in sensitivity and reproducibility, especially at low doses (&lt;\u20090.5\u00a0Gy) (practical limitations at low doses, including very low dicentric yields, higher statistical noise, and greater scoring uncertainty as aberration frequencies near background levels). This study presents a systematic comparison of pan-centromeric fluorescence in situ hybridization (pan-cent-FISH) and Giemsa staining for detecting dicentric (DC) and ring (R) chromosomes following\n                    <jats:sup>60<\/jats:sup>\n                    Co-\u03b3 irradiation (0\u20133\u00a0Gy). Analysis of more than 15,000 metaphases per technique revealed enhanced sensitivity of pan-cent-FISH technique, demonstrating a 1.72-fold higher linear coefficient and enhanced (1.13-fold) quadratic coefficient (\u03b2), indicating improved sensitivity across both low and high dose ranges. Blind validation with eight samples showed pan-cent-FISH achieved\u2009~\u20092-fold greater accuracy, with mean absolute differences of 0.0538\u00a0Gy (vs. 0.1105\u00a0Gy for Giemsa) and average relative errors of 7.13% (vs. 15.35% for Giemsa). At low doses (0.1\u00a0Gy), pan-cent-FISH maintained 9.0% error, while Giemsa exceeded acceptable limits (21.0% error). The standardized fluorescence detection used for the technique eliminated morphological ambiguities, reducing false negatives by ~\u200940% and improving first-pass accuracy.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-34407-3","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T21:53:07Z","timestamp":1770760387000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pan centromeric FISH enhances precision in radiation biodosimetry"],"prefix":"10.1038","volume":"16","author":[{"given":"Rajesh Kumar","family":"Chaurasia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aarti","family":"Notnani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devina Fenilon","family":"Vaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kapil B.","family":"Shirsath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheeri","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagesh N.","family":"Bhat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshad","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhruv","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balvinder K.","family":"Sapra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"34407_CR1","unstructured":"IAEA. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies (IAEA, 2011)."},{"key":"34407_CR2","unstructured":"ISO. ISO 19238:2023 Radiological Protection\u2014Performance Criteria for Service Laboratories Performing Biological Dosimetry by Cytogenetics - Dicentric Assay (International Organization for Standardization, 2023)."},{"key":"34407_CR3","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1007\/s00411-010-0311-3","volume":"49","author":"MMP de Lemos Pinto","year":"2010","unstructured":"de Lemos Pinto, M. M. P., Santos, N. F. G. & Amaral, A. Current status of biodosimetry based on standard cytogenetic methods. Radiat. Environ. Biophys. 49, 567\u2013581 (2010).","journal-title":"Radiat. Environ. Biophys."},{"issue":"12","key":"34407_CR4","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1080\/09553000600979092","volume":"82","author":"L Benkhaled","year":"2006","unstructured":"Benkhaled, L. et al. Analysis of \u03b3-rays induced chromosome aberrations: A fingerprint evaluation with a combination of pan-centromeric and pan-telomeric probes. Int. J. Radiat. Biol. 82 (12), 869\u2013875 (2006).","journal-title":"Int. J. Radiat. Biol."},{"key":"34407_CR5","doi-asserted-by":"crossref","unstructured":"M\u2019kacher, R. et al. New Tool for Biological Dosimetry: Reevaluation and Automation of the Gold Standard Method Following Telomere and Centromere Staining, vol. 770, 45\u201353 (Mutation Research\/Fundamental and Molecular Mechanisms of Mutagenesis, 2014).","DOI":"10.1016\/j.mrfmmm.2014.09.007"},{"issue":"2","key":"34407_CR6","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1667\/RR3231.1","volume":"180","author":"K Rothkamm","year":"2013","unstructured":"Rothkamm, K. et al. Comparison of established and emerging biodosimetry assays. Radiat. Res. 180 (2), 111\u2013119 (2013).","journal-title":"Radiat. Res."},{"issue":"3","key":"34407_CR7","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1080\/09553000010018693","volume":"77","author":"M Bauchinger","year":"2001","unstructured":"Bauchinger, M. et al. Collaborative exercise on the use of FISH chromosome painting for retrospective biodosimetry of Mayak nuclear-industrial personnel. Int. J. Radiat. Biol. 77 (3), 259\u2013267 (2001).","journal-title":"Int. J. Radiat. Biol."},{"key":"34407_CR8","doi-asserted-by":"crossref","unstructured":"M\u2019Kacher, R. et al. High resolution and automatable cytogenetic biodosimetry using in situ telomere and centromere hybridization for the accurate detection of DNA damage: An overview. Int. J. Mol. Sci. 24(6), 5699 (2023).","DOI":"10.3390\/ijms24065699"},{"issue":"3","key":"34407_CR9","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1016\/j.ijrobp.2014.10.048","volume":"91","author":"R M\u2019kacher","year":"2015","unstructured":"M\u2019kacher, R. et al. Detection and automated scoring of dicentric chromosomes in nonstimulated lymphocyte prematurely condensed chromosomes after telomere and centromere staining. Int. J. Radiat. Oncol. Biol. Phys. 91 (3), 640\u2013649 (2015).","journal-title":"Int. J. Radiat. Oncol. Biol. Phys."},{"issue":"8","key":"34407_CR10","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1080\/09553000802241770","volume":"84","author":"TS Fernandes","year":"2008","unstructured":"Fernandes, T. S., Lloyd, D. & Amaral, A. A comparison of different cytological stains for biological dosimetry. Int. J. Radiat. Biol. 84 (8), 703\u2013711 (2008).","journal-title":"Int. J. Radiat. Biol."},{"key":"34407_CR11","unstructured":"Lindholm, C., Salomaa, S. & Tekkel, M. Biodosimetry after accidental radiation exposure by conventional chromosome analysis of FISH. Occup. Health Ind. 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Establishment of ex vivo calibration curve for X-ray induced dicentric\u2009+\u2009ring and micronuclei in human peripheral lymphocytes for biodosimetry during radiological emergencies, and validation with dose blinded samples. Heliyon. 9(6). (2023).","DOI":"10.1016\/j.heliyon.2023.e17068"},{"key":"34407_CR15","doi-asserted-by":"crossref","unstructured":"Chaurasia, R. K. et al. FISH unveils a unified method for multi-marker biodose assessment. Sci. Rep. 15(1), 16994 (2025).","DOI":"10.1038\/s41598-025-87549-9"},{"key":"34407_CR16","doi-asserted-by":"crossref","unstructured":"Chaurasia, R. K. et al. First evidence of coexistence of Pseudo Pelger Huet anomaly and balanced translocation in a two decades retrospectively exposed human subject. Sci. 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Mutat. Res.\/Genet. Toxicol. Environ. Mutagen. 756 (1\u20132), 174\u2013183 (2013).","journal-title":"Mutat. Res.\/Genet. Toxicol. Environ. Mutagen."},{"issue":"1","key":"34407_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/rpd\/ncab106","volume":"195","author":"JW Marsh","year":"2021","unstructured":"Marsh, J. W., Tom\u00e1\u0161ek, L., Laurier, D. & Harrison, J. D. Effective dose coefficients for radon and progeny: a review of ICRP and UNSCEAR values. Radiat. Prot. Dosimetry. 195 (1), 1\u201320 (2021).","journal-title":"Radiat. Prot. Dosimetry"},{"issue":"5","key":"34407_CR21","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1667\/RR1664.1","volume":"171","author":"A Vaurijoux","year":"2009","unstructured":"Vaurijoux, A. et al. Strategy for population triage based on dicentric analysis. Radiat. Res. 171 (5), 541\u2013548 (2009).","journal-title":"Radiat. 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The project was conducted in accordance with the ethical principles outlined in the declaration. Informed ethical consent was obtained from the participating human volunteer following a comprehensive explanation of the project.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All authors have agreed to publish the manuscript in its present form.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"8020"}}