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Metal-based nanoparticles are emerging as a promising strategy to address this problem, which is the focus of the present work. Cu\n                    <jats:sub>0.15<\/jats:sub>\n                    Zn\n                    <jats:sub>0.2<\/jats:sub>\n                    Ni\n                    <jats:sub>0.65<\/jats:sub>\n                    Fe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    nano-ferrite was synthesized via the co-precipitation method. The chosen cation ratio preserves the spinel phase while Ni improves magnetic response, and Zn enhances magnetic softness and site stability. For comparison, single-cation ferrites NiFe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    , ZnFe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    , and CuFe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    were synthesized using the same procedure to enable a consistent evaluation of antibacterial activity. All ferrites were characterized using XRD and FTIR. Additional analyses including UV\u2013Vis, SEM, EDX, XPS, TEM, VSM, and Atomic Absorption Spectroscopy (AAS) were performed for Cu\n                    <jats:sub>0.15<\/jats:sub>\n                    Zn\n                    <jats:sub>0.2<\/jats:sub>\n                    Ni\n                    <jats:sub>0.65<\/jats:sub>\n                    Fe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    sample. XRD confirmed a cubic spinel phase for all ferrites. FTIR provided further evidence of cation redistribution of tetrahedral and octahedral sites. AAS verified the availability of Cu\n                    <jats:sup>2+<\/jats:sup>\n                    , Zn\n                    <jats:sup>2+<\/jats:sup>\n                    , and Ni\n                    <jats:sup>2+<\/jats:sup>\n                    ions, supporting their contribution to antibacterial activity. VSM showed soft magnetic behavior with\u2009~\u200954.3\u00a0emu\/g saturation magnetization. Antibacterial tests demonstrated that Cu\n                    <jats:sub>0.15<\/jats:sub>\n                    Zn\n                    <jats:sub>0.2<\/jats:sub>\n                    Ni\n                    <jats:sub>0.65<\/jats:sub>\n                    Fe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    exhibits stronger inhibitory activity against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>E. coli<\/jats:italic>\n                    at both low and high concentrations. At 500\u00a0\u03bcg\/mL, the inhibition zone reached\u2009~\u200920\u00a0mm for\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\u2009~\u200917\u00a0mm for\n                    <jats:italic>E. coli<\/jats:italic>\n                    , The MIC values were found to be 40\u00a0\u03bcg\/mL for\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and 80\u00a0\u03bcg\/mL for\n                    <jats:italic>E. coli<\/jats:italic>\n                    , indicating stronger sensitivity of Gram-positive bacteria. After establishing its individual performance, comparison has been obtained with single-cation ferrites. Across all trials, Cu\n                    <jats:sub>0.15<\/jats:sub>\n                    Zn\n                    <jats:sub>0.2<\/jats:sub>\n                    Ni\n                    <jats:sub>0.65<\/jats:sub>\n                    Fe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    consistently produced larger inhibition zones, showing clear superiority. The superior antibacterial activity is attributed to the synergistic incorporation of Cu\n                    <jats:sup>2+<\/jats:sup>\n                    , Zn\n                    <jats:sup>2+<\/jats:sup>\n                    , and Ni\n                    <jats:sup>2+<\/jats:sup>\n                    within a single spinel lattice, giving Cu\n                    <jats:sub>0.15<\/jats:sub>\n                    Zn\n                    <jats:sub>0.2<\/jats:sub>\n                    Ni\n                    <jats:sub>0.65<\/jats:sub>\n                    Fe\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    strong intrinsic antibacterial activity and improving performance over single-cation ferrites, confirming its novelty and potential for biomedical applications.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-34792-9","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T20:18:44Z","timestamp":1769113124000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Structural, optical, and magnetic characterization of Cu\u2013Zn\u2013Ni spinel ferrite nanoparticles with antibacterial potential"],"prefix":"10.1038","volume":"16","author":[{"given":"Samaa","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. 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