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Macroalgae may be a good alternative because of their biologically active metabolites with distinctive chemical structures that have been reported for their insecticidal properties. The study aimed to investigate the potential of different extracts from\n Jania rubens<\/jats:italic>\n (Linnaeus) J.V.Lamouroux and\n Colpomenia sinuosa<\/jats:italic>\n (Mertens ex Roth) Derb\u00e8s & Solier as genotoxic and larvicidal agents against\n Culex pipiens<\/jats:italic>\n Linnaeus larvae. The algae thallus was subjected to extraction using methanol, acetone, and methylene chloride. The phytochemical composition was quantified. The larvicidal activity of the different algae extracts was assessed against the third instar larvae of\n Culex pipiens<\/jats:italic>\n . Genotoxic evaluation through comet assays and compound characterization by GC\/MS was done. The results demonstrated that\n J. rubens<\/jats:italic>\n methylene chloride extract exhibited the highest contents of phenolics, alkaloids, flavonoids, terpenoids, and tannins while\n C. sinuosa<\/jats:italic>\n methanol extract demonstrated the highest levels of flavonoids, alkaloids, phenolics, and saponins. The larvicidal activity results revealed that\n J. rubens<\/jats:italic>\n methylene chloride extract was the most toxic for\n C. pipiens<\/jats:italic>\n larvae with LC\n 50<\/jats:sub>\n \u2009=\u20099.30 ppm followed by\n C. sinuosa<\/jats:italic>\n acetone extract with LC\n 50<\/jats:sub>\n \u2009=\u200982.58 ppm after 72\u00a0h of exposure. At 50 and 250 ppm,\n J. rubens<\/jats:italic>\n methylene chloride and\n C. sinuosa<\/jats:italic>\n acetone extracts maintained 100 and 93.33% larval mortality rates, respectively. Comet assay results demonstrated the genotoxicity of both algae extracts in\n C. pipiens<\/jats:italic>\n larvae. This study would be a beginning towards replacing chemical insecticides with algae-based bioinsecticides against\n C. pipiens<\/jats:italic>\n mosquitos.\n <\/jats:p>","DOI":"10.1038\/s41598-025-86347-7","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T06:40:59Z","timestamp":1737441659000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of some selected macroalgae extracts and assessment of their insecticidal and genotoxicity in Culex pipiens L. mosquito larvae"],"prefix":"10.1038","volume":"15","author":[{"given":"Dina A.","family":"Refaay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa M.","family":"El-Sheekh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin M.","family":"Heikal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Rashed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"86347_CR1","unstructured":"WHO. 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