{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:54:45Z","timestamp":1791482085789,"version":"4.3.4"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the Health Commission of Shaoxing, China","award":["2022KY040"],"award-info":[{"award-number":["2022KY040"]}]},{"name":"the Health Commission of Shaoxing, China","award":["2023SKY047"],"award-info":[{"award-number":["2023SKY047"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071729"],"award-info":[{"award-number":["82071729"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Technology Department of Zhejiang Province, China","award":["LTGY24H040006"],"award-info":[{"award-number":["LTGY24H040006"]}]},{"name":"Health Commission of Zhejiang Province, China","award":["2022KY1306"],"award-info":[{"award-number":["2022KY1306"]}]},{"name":"Science technology department of shaoxing, china","award":["2024A14004"],"award-info":[{"award-number":["2024A14004"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-87791-1","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T12:43:03Z","timestamp":1738240983000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Comprehensive profiling of serum microRNAs in normal and non-alcoholic fatty liver disease (NAFLD) patients"],"prefix":"10.1038","volume":"15","author":[{"given":"Jian-Wei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamran","family":"Ullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nauman","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Tao","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"87791_CR1","doi-asserted-by":"publisher","unstructured":"C, Y. et al. Comparison of serum exosome isolation methods on co-precipitated free microRNAs. PeerJ 8 https:\/\/doi.org\/10.7717\/peerj.9434 (2020).","DOI":"10.7717\/peerj.9434"},{"key":"87791_CR2","doi-asserted-by":"publisher","unstructured":"Zm, Z. Regulation of cellular miRNA expression by human papillomaviruses. Biochim. Biophys. Acta. 1809 https:\/\/doi.org\/10.1016\/j.bbagrm.2011.05.005 (2011).","DOI":"10.1016\/j.bbagrm.2011.05.005"},{"key":"87791_CR3","doi-asserted-by":"publisher","unstructured":"M S, B S, N T, et al. Widespread changes in protein synthesis induced by microRNAs. Nature ; 455. (2008). https:\/\/doi.org\/10.1038\/nature07228","DOI":"10.1038\/nature07228"},{"key":"87791_CR4","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1002\/1873-3468.14274","volume":"596","author":"JP Bischoff","year":"2022","unstructured":"Bischoff, J. P., Schulz, A. & Morrison, H. The role of exosomes in intercellular and inter-organ communication of the peripheral nervous system. FEBS Lett. 596, 655\u2013664. https:\/\/doi.org\/10.1002\/1873-3468.14274 (2022).","journal-title":"FEBS Lett."},{"key":"87791_CR5","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1007\/s13258-021-01150-8","volume":"44","author":"J-W Zhang","year":"2022","unstructured":"Zhang, J-W. & Pan, H-T. microRNA profiles of serum exosomes derived from children with nonalcoholic fatty liver. Genes Genomics. 44, 879\u2013888. https:\/\/doi.org\/10.1007\/s13258-021-01150-8 (2022).","journal-title":"Genes Genomics"},{"key":"87791_CR6","doi-asserted-by":"publisher","first-page":"1691","DOI":"10.1136\/gutjnl-2020-320622","volume":"69","author":"G Targher","year":"2020","unstructured":"Targher, G., Byrne, C. D. & Tilg, H. NAFLD and increased risk of cardiovascular disease: clinical associations, pathophysiological mechanisms and pharmacological implications. Gut 69, 1691\u20131705. https:\/\/doi.org\/10.1136\/gutjnl-2020-320622 (2020).","journal-title":"Gut"},{"key":"87791_CR7","doi-asserted-by":"publisher","unstructured":"Hk, M. & A, K. Increased hepatic synthesis and dysregulation of cholesterol metabolism is associated with the severity of nonalcoholic fatty liver disease. Cell Metabol. 15 https:\/\/doi.org\/10.1016\/j.cmet.2012.04.004 (2012).","DOI":"10.1016\/j.cmet.2012.04.004"},{"key":"87791_CR8","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1002\/hep.31050","volume":"72","author":"X-L Liu","year":"2020","unstructured":"Liu, X-L. et al. Lipotoxic hepatocyte-derived exosomal MicroRNA 192-5p activates macrophages through Rictor\/Akt\/Forkhead box transcription factor O1 signaling in nonalcoholic fatty liver disease. Hepatology 72, 454\u2013469. https:\/\/doi.org\/10.1002\/hep.31050 (2020).","journal-title":"Hepatology"},{"key":"87791_CR9","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.cca.2013.05.021","volume":"424","author":"H Yamada","year":"2013","unstructured":"Yamada, H. et al. Associations between circulating microRNAs (miR-21, miR-34a, miR-122 and miR-451) and non-alcoholic fatty liver. Clin. Chim. Acta. 424, 99\u2013103. https:\/\/doi.org\/10.1016\/j.cca.2013.05.021 (2013).","journal-title":"Clin. Chim. Acta"},{"key":"87791_CR10","doi-asserted-by":"publisher","first-page":"e0153497","DOI":"10.1371\/journal.pone.0153497","volume":"11","author":"NC Salvoza","year":"2016","unstructured":"Salvoza, N. C. et al. Association of Circulating Serum miR-34a and miR-122 with dyslipidemia among patients with non-alcoholic fatty liver disease. PLoS One. 11, e0153497. https:\/\/doi.org\/10.1371\/journal.pone.0153497 (2016).","journal-title":"PLoS One"},{"key":"87791_CR11","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1055\/a-2043-1044","volume":"55","author":"J Zhang","year":"2023","unstructured":"Zhang, J. et al. Prolactin is a key factor for nonalcoholic fatty liver disease in obese children. Horm. Metab. Res. 55, 251\u2013255. https:\/\/doi.org\/10.1055\/a-2043-1044 (2023).","journal-title":"Horm. Metab. Res."},{"key":"87791_CR12","doi-asserted-by":"publisher","first-page":"100685","DOI":"10.1016\/j.jhepr.2023.100685","volume":"5","author":"L Draijer","year":"2023","unstructured":"Draijer, L. et al. A natural history study of paediatric non-alcoholic fatty liver disease over 10 years. JHEP Rep. 5, 100685. https:\/\/doi.org\/10.1016\/j.jhepr.2023.100685 (2023).","journal-title":"JHEP Rep."},{"key":"87791_CR13","doi-asserted-by":"publisher","first-page":"4104","DOI":"10.3748\/wjg.v24.i36.4104","volume":"24","author":"J-L Torres","year":"2018","unstructured":"Torres, J-L. et al. Role of microRNAs in alcohol-induced liver disorders and non-alcoholic fatty liver disease. World J. Gastroenterol. 24, 4104\u20134118. https:\/\/doi.org\/10.3748\/wjg.v24.i36.4104 (2018).","journal-title":"World J. Gastroenterol."},{"key":"87791_CR14","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.jhep.2018.08.026","volume":"70","author":"T Zhang","year":"2019","unstructured":"Zhang, T. et al. MicroRNA-378 promotes hepatic inflammation and fibrosis via modulation of the NF-\u03baB-TNF\u03b1 pathway. J. Hepatol. 70, 87\u201396. https:\/\/doi.org\/10.1016\/j.jhep.2018.08.026 (2019).","journal-title":"J. Hepatol."},{"key":"87791_CR15","doi-asserted-by":"publisher","first-page":"803445","DOI":"10.3389\/fphys.2022.803445","volume":"13","author":"Y Hu","year":"2022","unstructured":"Hu, Y. et al. MicroRNA-122-5p inhibition improves inflammation and oxidative stress damage in Dietary-Induced non-alcoholic fatty liver Disease through Targeting FOXO3. Front. Physiol. 13, 803445. https:\/\/doi.org\/10.3389\/fphys.2022.803445 (2022).","journal-title":"Front. Physiol."},{"key":"87791_CR16","doi-asserted-by":"publisher","first-page":"38262","DOI":"10.1038\/srep38262","volume":"6","author":"E Raitoharju","year":"2016","unstructured":"Raitoharju, E. et al. Blood hsa-mir-122-5p and hsa-mir-885-5p levels associate with fatty liver and related lipoprotein metabolism-the Young finns Study. Sci. Rep. 6, 38262. https:\/\/doi.org\/10.1038\/srep38262 (2016).","journal-title":"Sci. Rep."},{"key":"87791_CR17","doi-asserted-by":"publisher","unstructured":"Gn, L-S. et al. Hepatic mir-122-3p, mir-140-5p and mir-148b-5p expressions are correlated with cytokeratin-18 serum levels in MAFLD. Ann. Hepatol. 27. https:\/\/doi.org\/10.1016\/j.aohep.2022.100756 (2022).","DOI":"10.1016\/j.aohep.2022.100756"},{"key":"87791_CR18","doi-asserted-by":"publisher","unstructured":"K, M. Toll-like receptor 9 promotes steatohepatitis by induction of interleukin-1beta in mice. Gastroenterology 139. https:\/\/doi.org\/10.1053\/j.gastro.2010.03.052 (2010).","DOI":"10.1053\/j.gastro.2010.03.052"},{"key":"87791_CR19","doi-asserted-by":"publisher","unstructured":"N D, L W, B K, et al. A single nucleotide polymorphism of toll-like receptor 4 identifies the risk of developing graft failure after liver transplantation. J. Hepatol. ; 53. (2010). https:\/\/doi.org\/10.1016\/j.jhep.2009.12.044","DOI":"10.1016\/j.jhep.2009.12.044"},{"key":"87791_CR20","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1080\/10409238.2018.1448752","volume":"53","author":"MV Machado","year":"2018","unstructured":"Machado, M. V. & Diehl, A. M. The hedgehog pathway in nonalcoholic fatty liver disease. Crit. Rev. Biochem. Mol. Biol. 53, 264\u2013278. https:\/\/doi.org\/10.1080\/10409238.2018.1448752 (2018).","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"87791_CR21","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1016\/j.jhep.2017.10.017","volume":"68","author":"MV Machado","year":"2018","unstructured":"Machado, M. V. & Diehl, A. M. Hedgehog signaling in liver pathophysiology. J. Hepatol. 68, 550\u2013562. https:\/\/doi.org\/10.1016\/j.jhep.2017.10.017 (2018).","journal-title":"J. Hepatol."},{"key":"87791_CR22","doi-asserted-by":"publisher","unstructured":"Ar, L-P. et al. Concerted regulation of non-alcoholic fatty liver disease progression by microRNAs in apolipoprotein E-deficient mice. Dis. Models Mech. 14 https:\/\/doi.org\/10.1242\/dmm.049173 (2021).","DOI":"10.1242\/dmm.049173"},{"key":"87791_CR23","doi-asserted-by":"publisher","first-page":"1675","DOI":"10.2147\/DMSO.S448165","volume":"17","author":"K Yang","year":"2024","unstructured":"Yang, K. et al. Would Combination be Better: Swimming Exercise and Intermittent Fasting Improve High-Fat Diet-Induced nonalcoholic fatty liver disease in obese rats via the miR-122-5p\/SREBP-1c\/CPT1A pathway. Diabetes Metab. Syndr. Obes. 17, 1675\u20131686. https:\/\/doi.org\/10.2147\/DMSO.S448165 (2024).","journal-title":"Diabetes Metab. Syndr. Obes."},{"key":"87791_CR24","doi-asserted-by":"publisher","first-page":"118816","DOI":"10.1016\/j.lfs.2020.118816","volume":"265","author":"X Wu","year":"2021","unstructured":"Wu, X. et al. Inhibition of miR-122 reduced atherosclerotic lesion formation by regulating NPAS3-mediated endothelial to mesenchymal transition. Life Sci. 265, 118816. https:\/\/doi.org\/10.1016\/j.lfs.2020.118816 (2021).","journal-title":"Life Sci."},{"key":"87791_CR25","doi-asserted-by":"publisher","first-page":"e22922","DOI":"10.1016\/j.heliyon.2023.e22922","volume":"9","author":"H Zhao","year":"2023","unstructured":"Zhao, H. et al. MiR-122-5p as a potential regulator of pulmonary vascular wall cell in idiopathic pulmonary arterial hypertension. Heliyon 9, e22922. https:\/\/doi.org\/10.1016\/j.heliyon.2023.e22922 (2023).","journal-title":"Heliyon"},{"key":"87791_CR26","doi-asserted-by":"publisher","first-page":"110276","DOI":"10.1016\/j.cbi.2022.110276","volume":"369","author":"ZM Abdel-Nasser","year":"2023","unstructured":"Abdel-Nasser, Z. M., Zaafan, M. A. & Abdelhamid, A. M. Modulation of the miR-122\/Sirt-6\/ACE2 axis on experimentally-induced myocardial infarction. Chem. Biol. Interact. 369, 110276. https:\/\/doi.org\/10.1016\/j.cbi.2022.110276 (2023).","journal-title":"Chem. Biol. Interact."},{"key":"87791_CR27","doi-asserted-by":"publisher","first-page":"3332","DOI":"10.1021\/acs.jmedchem.1c01831","volume":"65","author":"RR Gaddam","year":"2022","unstructured":"Gaddam, R. R. et al. \u03b3 peptide nucleic acid-based miR-122 inhibition rescues vascular endothelial dysfunction in mice Fed a High-Fat Diet. J. Med. Chem. 65, 3332\u20133342. https:\/\/doi.org\/10.1021\/acs.jmedchem.1c01831 (2022).","journal-title":"J. Med. Chem."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-87791-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-87791-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-87791-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T12:43:14Z","timestamp":1738240994000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-87791-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,30]]},"references-count":27,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["87791"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-87791-1","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,30]]},"assertion":[{"value":"17 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 January 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"For research involving human participants who are minors, including tissue sample donors, informed consent was obtained from a parent and\/or legal guardian.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"This study received approval from the Shaoxing Maternal and Child Health Care Hospital\u2019s Ethics Committee. Everyone who participated in the study gave informed consent.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}],"article-number":"3766"}}