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While microscopy is still the gold standard in malaria diagnosis, it relies heavily on trained personnel. Artificial intelligence (AI) advances, particularly convolutional neural networks (CNNs), have significantly improved diagnostic capabilities and accuracy by enabling the automated analysis of medical images. Previous models efficiently detected malaria parasites in red blood cells but had difficulty differentiating between species. We propose a CNN-based model for classifying cells infected by\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    ,\n                    <jats:italic>P. vivax<\/jats:italic>\n                    , and uninfected white blood cells from thick blood smears. Our best-performing model utilizes a seven-channel input and correctly predicted 12,876 out of 12,954 cases. We also generated a cross-validation confusion matrix that showed the results of five iterations, achieving 63,654 out of 64,126 true predictions. The model\u2019s accuracy reached 99.51%, a precision of 99.26%, a recall of 99.26%, a specificity of 99.63%, an F1 score of 99.26%, and a loss of 2.3%. We are now developing a system based on real-world quality images to create a comprehensive detection tool for remote regions where trained microscopists are unavailable.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-87979-5","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T04:23:16Z","timestamp":1738210996000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Deep learning-based malaria parasite detection: convolutional neural networks model for accurate species identification of Plasmodium falciparum and Plasmodium vivax"],"prefix":"10.1038","volume":"15","author":[{"given":"Diego A.","family":"Ramos-Brice\u00f1o","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Flammia-D\u2019Aleo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"Fern\u00e1ndez-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fhabi\u00e1n S.","family":"Carri\u00f3n-Nessi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Forero-Pe\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"87979_CR1","doi-asserted-by":"publisher","first-page":"e002023","DOI":"10.1136\/bmjgh-2019-002023","volume":"5","author":"E Macarayan","year":"2020","unstructured":"Macarayan, E., Papanicolas, I. & Jha, A. 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