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The effect of increasing temperature on the binodal curves was investigated. The experimental binodal data were correlated using two empirical nonlinear three parameter expressions developed by Merchuk, and Zafarani-Moattar et al. and the two-parameter modified equation derived from effective excluded volume theory. Then, the experimental tie-line data were represented with Othmer-Tobias and Setschenow correlations. Finally, in these ATPSs the partition behavior of analgesic drugs, namely acetaminophen, salicylic acid, and ceftriaxone were investigated by measuring of partition coefficient and extraction efficiency. The trend of obtained results showed that the selected drugs were successfully extracted into the top phase of studied ATPSs with a partition coefficient greater than one and in the salt systems extraction efficiency was obtained up to 98% and in the case of polymer systems was obtained up to 8% at different tie-lines and it was found that the hydrophobicity of partitioned drugs is responsible for this phenomenon. <\/jats:p>","DOI":"10.1038\/s41598-025-88326-4","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T14:29:33Z","timestamp":1739456973000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Phase equilibria and drug partitioning ability of betaine based aqueous two-phase systems"],"prefix":"10.1038","volume":"15","author":[{"given":"Mohammed Taghi","family":"Zafarani-Moattar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemayat","family":"Shekaari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soheila","family":"Asadollahi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"88326_CR1","doi-asserted-by":"publisher","first-page":"12349","DOI":"10.1039\/D3RA07066B","volume":"14","author":"F Ghaffari","year":"2024","unstructured":"Ghaffari, F., Zafarani-Moattar, T., Shekaari, H. & M. & Harnessing the power of natural deep eutectic solvents (choline chloride\/sucrose) and polypropylene glycol in the formation of aqueous biphasic systems and the application of these systems in drug extraction. 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