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Here we report on post-mortem imaging of excised non-human primate and rodent cochleae at different <jats:inline-formula>\n              <jats:tex-math>$${\\upmu }$$<\/jats:tex-math>\n            <\/jats:inline-formula>-CT and nano-CT synchrotron instruments. We explore different sample embeddings, stainings and imaging regimes. Under optimized conditions of sample preparation, instrumentation, imaging protocol, and phase retrieval, high image quality and detail level can be achieved in 3D reconstructions. The showcased instrumentation and imaging protocols along with the reconstucted volumes can serve as benchmarks and reference for multi-scale microanatomy and 3D histology. 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