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This study evaluated the response of <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> to HFEMWs across a frequency range of 900\u00a0MHz to 73\u00a0GHz. The Bacterial sensitivity to antibiotics, including ceftazidime, ceftaroline, gentamycin, doxycycline, and ciprofloxacin, was assessed. The growth rate was evaluated by measuring the optical density (OD) and the number of colony-forming units (CFUs). Our results revealed significant electromagnetic interference (EMI) effects at frequencies of 51.8\u00a0GHz and 53\u00a0GHz, with 53\u00a0GHz showing the most pronounced impact. These frequencies enhanced bacterial susceptibility, with previously resistant <jats:italic>E. coli<\/jats:italic> and <jats:italic>S. aureus<\/jats:italic> strains becoming sensitive to tested antibiotics. Conversely, 70.6\u00a0GHz and 73\u00a0GHz frequencies showed limited effects, while exposure to 900\u00a0MHz and 1800\u00a0MHz caused no notable changes. These findings highlight the frequency-dependent effects of HFEMWs on bacterial viability and antibiotic sensitivity. 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