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This study was performed to explore the role of ALKBH1 in hypoxia treated human extravasated trophoblast cells. Hypoxia treated human extravasated trophoblast cells (HTR-8\/SVneo) was used to simulate the occurrence of PE in vitro. The cells phenotype was detected by CCK-8 and Transwell assays. The m5c levels and m5C levels of PSMD14 were analyzed by m5C dot blot and M5C Me-RIP assays. Then, the interaction between ALKBH1 and PSMD14 were confirmed by RIP and dual-luciferase reporter assays. ALKBH1 was up-regulated in hypoxia treated HTR-8\/SVneo cells. Additionally, ALKBH1 knockdown increased the m5C contents, cell viability, migration and invasion abilities of hypoxia treated HTR-8\/SVneo cells. Furthermore, ALKBH1 knockdown increased the m5C and mRNA levels, and mRNA stability of PSMD14. RIP and dual-luciferase reporter assays demonstrated that ALKBH1 interacted with PSMD14. Besides, PSMD14 knockdown reversed the effects of ALKBH1 silencing on cell viability, migration and invasion abilities of hypoxia treated HTR-8\/SVneo cells. ALKBH1 mediated m5C levels were decreased in the hypoxia treated HTR-8\/SVneo cells, which further decreased the cell viability, migration and invasion abilities through targeting the PSMD14 levels.<\/jats:p>","DOI":"10.1038\/s41598-025-91233-3","type":"journal-article","created":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T15:20:36Z","timestamp":1740842436000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["ALKBH1 knockdown promotes the growth, migration and invasion of HTR-8\/SVneo cells through regulating the m5C modification PSMD14"],"prefix":"10.1038","volume":"15","author":[{"given":"Caili","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peifeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,1]]},"reference":[{"key":"91233_CR1","doi-asserted-by":"publisher","first-page":"S1211","DOI":"10.1016\/j.ajog.2020.10.027","volume":"226","author":"A Hauspurg","year":"2022","unstructured":"Hauspurg, A. & Jeyabalan, A. 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