{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:35:03Z","timestamp":1790613303910,"version":"4.1.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100003725","name":"The National Research Foundation of Korea","doi-asserted-by":"crossref","award":["2022R1I1A1A01063976"],"award-info":[{"award-number":["2022R1I1A1A01063976"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"The National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2023-00207833"],"award-info":[{"award-number":["RS-2023-00207833"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Korea Health Industry Development Institute","award":["HR20C0021"],"award-info":[{"award-number":["HR20C0021"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-92935-4","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T08:19:51Z","timestamp":1741767591000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of a rapid malaria LAMP-MS assay for diagnosis of malaria infections"],"prefix":"10.1038","volume":"15","author":[{"given":"Woong Sik","family":"Jang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Kyeong","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Lan","family":"Choe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7765-0840","authenticated-orcid":false,"given":"Chae Seung","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"key":"92935_CR1","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1038\/s41586-019-0973-1","volume":"567","author":"DG Paton","year":"2019","unstructured":"Paton, D. G. et al. Exposing Anopheles mosquitoes to antimalarials blocks plasmodium parasite transmission. Nature 567, 239\u2013243 (2019).","journal-title":"Nature"},{"key":"92935_CR2","doi-asserted-by":"publisher","first-page":"e3071","DOI":"10.1371\/journal.pntd.0003071","volume":"8","author":"C Naing","year":"2014","unstructured":"Naing, C., Whittaker, M. A., Nyunt Wai, V. & Mak, J. W. Is plasmodium Vivax malaria a severe malaria? A systematic review and Meta-Analysis. PLoS Negl. Trop. Dis. 8, e3071 (2014).","journal-title":"PLoS Negl. Trop. Dis."},{"key":"92935_CR3","doi-asserted-by":"publisher","first-page":"e0001061","DOI":"10.1371\/journal.pgph.0001061","volume":"2","author":"GR Gore-Langton","year":"2022","unstructured":"Gore-Langton, G. R. et al. Global estimates of pregnancies at risk of plasmodium falciparum and plasmodium Vivax infection in 2020 and changes in risk patterns since 2000. PLOS Global Public. Health. 2, e0001061 (2022).","journal-title":"PLOS Global Public. Health"},{"key":"92935_CR4","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.4269\/ajtmh.22-0128","volume":"106","author":"PJ Rosenthal","year":"2022","unstructured":"Rosenthal, P. J. Malaria in 2022: challenges and progress. Am. J. Trop. Med. Hyg. 106, 1565\u20131567 (2022).","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"92935_CR5","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1186\/s12936-016-1290-x","volume":"15","author":"D Roberts","year":"2016","unstructured":"Roberts, D. & Matthews, G. Risk factors of malaria in children under the age of five years old in Uganda. Malar. J. 15, 246 (2016).","journal-title":"Malar. J."},{"key":"92935_CR6","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1016\/j.socscimed.2008.05.013","volume":"67","author":"L Stratton","year":"2008","unstructured":"Stratton, L., O\u2019Neill, M. S., Kruk, M. E. & Bell, M. L. The persistent problem of malaria: addressing the fundamental causes of a global killer. Soc. Sci. Med. 67, 854\u2013862 (2008).","journal-title":"Soc. Sci. Med."},{"key":"92935_CR7","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1186\/s12936-016-1399-y","volume":"15","author":"J Landier","year":"2016","unstructured":"Landier, J. et al. The role of early detection and treatment in malaria elimination. Malar. J. 15, 363 (2016).","journal-title":"Malar. J."},{"key":"92935_CR8","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1186\/cc2183","volume":"7","author":"A Trampuz","year":"2003","unstructured":"Trampuz, A., Jereb, M., Muzlovic, I. & Prabhu, R. M. Clinical review: severe malaria. Crit. Care. 7, 315 (2003).","journal-title":"Crit. Care"},{"key":"92935_CR9","doi-asserted-by":"publisher","first-page":"78663","DOI":"10.1109\/ACCESS.2020.2990497","volume":"8","author":"W Kudisthalert","year":"2020","unstructured":"Kudisthalert, W., Pasupa, K. & Tongsima, S. Counting and classification of malarial parasite from Giemsa-Stained thin film images. IEEE Access. 8, 78663\u201378682 (2020).","journal-title":"IEEE Access."},{"key":"92935_CR10","doi-asserted-by":"publisher","first-page":"45","DOI":"10.4269\/ajtmh.1995.52.45","volume":"52","author":"R Dietze","year":"1995","unstructured":"Dietze, R. et al. The diagnosis of plasmodium falciparum infection using a new antigen detection system. Am. J. Trop. Med. Hyg. 52, 45\u201349 (1995).","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"92935_CR11","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.tmaid.2007.10.001","volume":"6","author":"LK Erdman","year":"2008","unstructured":"Erdman, L. K. & Kain, K. C. Molecular diagnostic and surveillance tools for global malaria control. Travel Med. Infect. Dis. 6, 82\u201399 (2008).","journal-title":"Travel Med. Infect. Dis."},{"key":"92935_CR12","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1179\/136485910X12607012373993","volume":"104","author":"DRS Ishengoma","year":"2010","unstructured":"Ishengoma, D. R. S. et al. The performance of health laboratories and the quality of malaria diagnosis in six districts of Tanzania. Ann. Trop. Med. Parasitol. 104, 123\u2013135 (2010).","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"92935_CR13","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1159\/000070751","volume":"12","author":"J Iqbal","year":"2003","unstructured":"Iqbal, J., Hira, P. R., Al-Ali, F., Khalid, N. & Sher, A. Modified Giemsa staining for rapid diagnosis of malaria infection. Med. Principles Pract. 12, 156\u2013159 (2003).","journal-title":"Med. Principles Pract."},{"key":"92935_CR14","doi-asserted-by":"publisher","first-page":"012090","DOI":"10.1088\/1755-1315\/125\/1\/012090","volume":"125","author":"L Siahaan","year":"2018","unstructured":"Siahaan, L. Laboratory diagnostics of malaria. IOP Conf. Ser. Earth Environ. Sci. 125, 012090 (2018).","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"92935_CR15","doi-asserted-by":"publisher","first-page":"527","DOI":"10.3390\/diagnostics11030527","volume":"11","author":"J Yoon","year":"2021","unstructured":"Yoon, J., Jang, W. S., Nam, J., Mihn, D. C. & Lim, C. S. An automated microscopic malaria parasite detection system using digital image analysis. Diagnostics 11, 527 (2021).","journal-title":"Diagnostics"},{"key":"92935_CR16","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1186\/1475-2875-10-176","volume":"10","author":"DS Ishengoma","year":"2011","unstructured":"Ishengoma, D. S. et al. Accuracy of malaria rapid diagnostic tests in community studies and their impact on treatment of malaria in an area with declining malaria burden in north-eastern Tanzania. Malar. J. 10, 176 (2011).","journal-title":"Malar. J."},{"key":"92935_CR17","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1128\/CMR.15.1.66-78.2002","volume":"15","author":"A Moody","year":"2002","unstructured":"Moody, A. Rapid diagnostic tests for malaria parasites. Clin. Microbiol. Rev. 15, 66\u201378 (2002).","journal-title":"Clin. Microbiol. Rev."},{"key":"92935_CR18","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1186\/1471-2458-12-291","volume":"12","author":"DJ Kyabayinze","year":"2012","unstructured":"Kyabayinze, D. J. et al. Programme level implementation of malaria rapid diagnostic tests (RDTs) use: outcomes and cost of training health workers at lower level health care facilities in Uganda. BMC Public. Health. 12, 291 (2012).","journal-title":"BMC Public. Health"},{"key":"92935_CR19","doi-asserted-by":"publisher","first-page":"1624","DOI":"10.1111\/j.1469-0691.2011.03639.x","volume":"17","author":"ML McMorrow","year":"2011","unstructured":"McMorrow, M. L., Aidoo, M. & Kachur, S. P. Malaria rapid diagnostic tests in elimination settings\u2014can they find the last parasite? Clin. Microbiol. Infect. 17, 1624\u20131631 (2011).","journal-title":"Clin. Microbiol. Infect."},{"key":"92935_CR20","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.actatropica.2009.02.003","volume":"111","author":"MG Cunha","year":"2009","unstructured":"Cunha, M. G. et al. Development of a polymerase chain reaction (PCR) method based on amplification of mitochondrial DNA to detect plasmodium falciparum and plasmodium Vivax. Acta Trop. 111, 35\u201338 (2009).","journal-title":"Acta Trop."},{"key":"92935_CR21","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S1383-5769(97)00013-5","volume":"46","author":"M Kimura","year":"1997","unstructured":"Kimura, M. et al. Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene. Parasitol. Int. 46, 91\u201395 (1997).","journal-title":"Parasitol. Int."},{"key":"92935_CR22","doi-asserted-by":"publisher","first-page":"1491","DOI":"10.1099\/jmm.0.052506-0","volume":"62","author":"JC Mouatcho","year":"2013","unstructured":"Mouatcho, J. C. & Goldring, J. P. D. Malaria rapid diagnostic tests: challenges and prospects. J. Med. Microbiol. 62, 1491\u20131505 (2013).","journal-title":"J. Med. Microbiol."},{"key":"92935_CR23","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1186\/s12936-016-1158-0","volume":"15","author":"S Britton","year":"2016","unstructured":"Britton, S., Cheng, Q. & McCarthy, J. S. Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings. Malar. J. 15, 88 (2016).","journal-title":"Malar. J."},{"key":"92935_CR24","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1080\/14737159.2018.1431529","volume":"18","author":"NW Lucchi","year":"2018","unstructured":"Lucchi, N. W., Ndiaye, D., Britton, S. & Udhayakumar, V. Expanding the malaria molecular diagnostic options: opportunities and challenges for loop-mediated isothermal amplification tests for malaria control and elimination. Expert Rev. Mol. Diagn. 18, 195\u2013203 (2018).","journal-title":"Expert Rev. Mol. Diagn."},{"key":"92935_CR25","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.actatropica.2012.02.004","volume":"122","author":"R Abdul-Ghani","year":"2012","unstructured":"Abdul-Ghani, R., Al-Mekhlafi, A. M. & Karanis, P. Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: would it come to clinical reality as a point-of-care test? Acta Trop. 122, 233\u2013240 (2012).","journal-title":"Acta Trop."},{"key":"92935_CR26","doi-asserted-by":"publisher","first-page":"e0142842","DOI":"10.1371\/journal.pone.0142842","volume":"10","author":"J Cuadros","year":"2015","unstructured":"Cuadros, J. et al. Field evaluation of malaria microscopy, rapid malaria tests and Loop-Mediated isothermal amplification in a rural hospital in South Western Ethiopia. PLoS One. 10, e0142842 (2015).","journal-title":"PLoS One"},{"key":"92935_CR27","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.tmaid.2014.12.016","volume":"13","author":"H Marti","year":"2015","unstructured":"Marti, H., Stalder, C. & Gonz\u00e1lez, I. J. Diagnostic accuracy of a LAMP kit for diagnosis of imported malaria in Switzerland. Travel Med. Infect. Dis. 13, 167\u2013171 (2015).","journal-title":"Travel Med. Infect. Dis."},{"key":"92935_CR28","doi-asserted-by":"publisher","first-page":"108310","DOI":"10.1016\/j.exppara.2022.108310","volume":"239","author":"LPY Zen","year":"2022","unstructured":"Zen, L. P. Y. et al. End-point detection of loop-mediated isothermal amplification (LAMP) on malaria by direct observation with colorimetric dyes. Exp. Parasitol. 239, 108310 (2022).","journal-title":"Exp. Parasitol."},{"key":"92935_CR29","doi-asserted-by":"publisher","unstructured":"Kongkasuriyachai, D., Yongkiettrakul, S., Kiatpathomchai, W. & Arunrut, N. Loop-Mediated isothermal amplification and LFD combination for detection of Plasmodium falciparum and Plasmodium vivax, 431\u2013443 (2017). https:\/\/doi.org\/10.1007\/978-1-4939-6911-1_28","DOI":"10.1007\/978-1-4939-6911-1_28"},{"key":"92935_CR30","doi-asserted-by":"publisher","first-page":"e13733","DOI":"10.1371\/journal.pone.0013733","volume":"5","author":"NW Lucchi","year":"2010","unstructured":"Lucchi, N. W. et al. Real-Time fluorescence loop mediated isothermal amplification for the diagnosis of malaria. PLoS One. 5, e13733 (2010).","journal-title":"PLoS One"},{"key":"92935_CR31","doi-asserted-by":"publisher","first-page":"348","DOI":"10.3390\/bios14070348","volume":"14","author":"M Choi","year":"2024","unstructured":"Choi, M., Lee, E., Park, S., Lim, C. S. & Jang, W. S. Enhanced Point-of-Care SARS-CoV-2 detection: integrating RT-LAMP with microscanning. Biosens. (Basel). 14, 348 (2024).","journal-title":"Biosens. (Basel)"},{"key":"92935_CR32","doi-asserted-by":"publisher","first-page":"1950","DOI":"10.3390\/diagnostics11111950","volume":"11","author":"WS Jang","year":"2021","unstructured":"Jang, W. S. et al. Development of a multiplex Loop-Mediated isothermal amplification assay for diagnosis of plasmodium spp., plasmodium falciparum and plasmodium Vivax. Diagnostics 11, 1950 (2021).","journal-title":"Diagnostics"},{"key":"92935_CR33","doi-asserted-by":"publisher","unstructured":"Patel, P., Bagada, A. & Vadia, N. Epidemiology and current trends in malaria. In Rising Contagious Diseases,  261\u2013282 (Wiley, 2024). https:\/\/doi.org\/10.1002\/9781394188741.ch20.","DOI":"10.1002\/9781394188741.ch20"},{"key":"92935_CR34","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1186\/s12936-017-1925-6","volume":"16","author":"J Recht","year":"2017","unstructured":"Recht, J. et al. Malaria in Brazil, Colombia, Peru and Venezuela: current challenges in malaria control and elimination. Malar. J. 16, 273 (2017).","journal-title":"Malar. J."},{"key":"92935_CR35","doi-asserted-by":"publisher","first-page":"115122","DOI":"10.1016\/j.diagmicrobio.2020.115122","volume":"98","author":"SL Rei Yan","year":"2020","unstructured":"Rei Yan, S. L., Wakasuqui, F. & Wrenger, C. Point-of-care tests for malaria: speeding up the diagnostics at the bedside and challenges in malaria cases detection. Diagn. Microbiol. Infect. Dis. 98, 115122 (2020).","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"92935_CR36","doi-asserted-by":"publisher","first-page":"636","DOI":"10.1128\/JCM.42.2.636-638.2004","volume":"42","author":"GA Farcas","year":"2004","unstructured":"Farcas, G. A., Zhong, K. J. Y., Mazzulli, T. & Kain, K. C. Evaluation of the RealArt malaria LC Real-Time PCR assay for malaria diagnosis. J. Clin. Microbiol. 42, 636\u2013638 (2004).","journal-title":"J. Clin. Microbiol."},{"key":"92935_CR37","doi-asserted-by":"crossref","unstructured":"Lyimo, B. M. et al. Potential opportunities and challenges of deploying next generation sequencing and CRISPR-Cas systems to support diagnostics and surveillance towards malaria control and elimination in Africa. Front. Cell. Infect. Microbiol. 12 (2022).","DOI":"10.3389\/fcimb.2022.757844"},{"key":"92935_CR38","doi-asserted-by":"publisher","first-page":"29","DOI":"10.4103\/0972-9062.331409","volume":"59","author":"S Sharma","year":"2022","unstructured":"Sharma, S., Singh, J., Sen, A. & Anvikar, A. R. Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria. J. Vector Borne Dis. 59, 29\u201336 (2022).","journal-title":"J. Vector Borne Dis."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-92935-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-92935-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-92935-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T18:17:38Z","timestamp":1741803458000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-92935-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":38,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["92935"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-92935-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,12]]},"assertion":[{"value":"30 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This study was approved by the Medical Ethics Committee of Korea University\u2019s Guro Hospital (2021GR0337).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Patient informed consent was waived by the Institutional Review Board of Korea University Guro Hospital, as the identities of the subjects were completely anonymous, and there was minimal risk involved in the study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}],"article-number":"8547"}}