{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T03:08:09Z","timestamp":1794280089525,"version":"4.9.0"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100009171","name":"Natural Resources Conservation Service","doi-asserted-by":"publisher","award":["NR233A750011G019"],"award-info":[{"award-number":["NR233A750011G019"]}],"id":[{"id":"10.13039\/100009171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-93198-9","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T13:05:11Z","timestamp":1741957511000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Synergistic effects of biochar and plants can reduce greenhouse gas emissions from salt affected soil"],"prefix":"10.1038","volume":"15","author":[{"given":"Dwarika","family":"Bhattarai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailesh","family":"Pandit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rojina","family":"Kafle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thandiwe","family":"Nleya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Clay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon A.","family":"Clay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"93198_CR1","doi-asserted-by":"publisher","DOI":"10.1002\/9780891183976","volume-title":"Salinity and Sodicity: A Growing Global Challenge to Food Security, Environmental Quality and Soil Resilience","author":"DE Clay","year":"2024","unstructured":"Clay, D. E., DeSutter, T. M., Clay, S. A. & Nleya, T. Salinity and Sodicity: A Growing Global Challenge to Food Security, Environmental Quality and Soil Resilience (Wiley, 2024)."},{"key":"93198_CR2","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1002\/jeq2.20223","volume":"50","author":"DJ Fiedler","year":"2021","unstructured":"Fiedler, D. J. et al. CO2 and N2O emissions and microbial community structure from fields that include salt-affected soils. J. Environ. Qual. 50, 567\u2013579. https:\/\/doi.org\/10.1002\/jeq2.20223 (2021).","journal-title":"J. Environ. Qual."},{"key":"93198_CR3","unstructured":"Eggleston, H., Buendia, L., Miwa, K., Ngara, T. & Tanabe, K. 2006 IPCC guidelines for national greenhouse gas inventories. Institute for Global Environmental Strategies (IGES) for the IPCC https:\/\/www.ipcc.ch\/report\/2006-ipcc-guidelines-for-national-greenhouse-gas-inventories\/  (2006)."},{"key":"93198_CR4","doi-asserted-by":"crossref","unstructured":"Clay, D. E., Pandit, S. & Bhattarai, D. Case studies on salt-affected soil remediation. In Salinity and Sodicity: A Growing Global Challenge to Food Security, Environmental Quality and Soil Resilience (eds. Clay, D.E., DeSutter, T.M., Clay, S.A. & Nleya, T.) 129\u2013142 (Wiley, 2024).","DOI":"10.1002\/9780891183976.ch15"},{"key":"93198_CR5","doi-asserted-by":"publisher","first-page":"418","DOI":"10.2489\/jswc.2022.00131","volume":"77","author":"M Budak","year":"2022","unstructured":"Budak, M. et al. Increased rainfall may place saline\/sodic soils on the tipping point of sustainability. J. Soil Water Conserv. 77, 418\u2013425 (2022).","journal-title":"J. Soil Water Conserv."},{"key":"93198_CR6","doi-asserted-by":"publisher","first-page":"496","DOI":"10.2134\/agronj2018.04.0296","volume":"111","author":"GA Birru","year":"2019","unstructured":"Birru, G. A. et al. Chemical amendments of dryland saline\u2013sodic soils did not enhance productivity and soil health in fields without effective drainage. Agron. J. 111, 496\u2013508 (2019).","journal-title":"Agron. J."},{"key":"93198_CR7","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1002\/agj2.21185","volume":"116","author":"DR Joshi","year":"2024","unstructured":"Joshi, D. R. et al. Quantification and machine learning based N2O\u2013N and CO2\u2013C emissions predictions from a decomposing rye cover crop. Agron. J. 116, 795\u2013809 (2024).","journal-title":"Agron. J."},{"key":"93198_CR8","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1002\/agj2.21573","volume":"116","author":"SA Clay","year":"2024","unstructured":"Clay, S. A. et al. Plants reduced nitrous oxide emissions from a Northern Great Plains saline\/sodic soil. Agron. J. 116, 1343\u20131356 (2024).","journal-title":"Agron. J."},{"key":"93198_CR9","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.2136\/sssaj1984.03615995004800060013x","volume":"48","author":"DM Linn","year":"1984","unstructured":"Linn, D. M. & Doran, J. W. Effect of water-filled pore space on carbon dioxide and nitrous oxide production in tilled and nontilled soils. Soil Sci. Soc. Am. J. 48, 1267\u20131272. https:\/\/doi.org\/10.2136\/sssaj1984.03615995004800060013x (1984).","journal-title":"Soil Sci. Soc. Am. J."},{"key":"93198_CR10","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1002\/clen.201300089","volume":"42","author":"R Chintala","year":"2014","unstructured":"Chintala, R. et al. Phosphorus sorption and availability from biochars and soil\/B iochar mixtures. CLEAN Soil Air Water 42, 626\u2013634 (2014).","journal-title":"CLEAN Soil Air Water"},{"key":"93198_CR11","first-page":"1","volume":"12","author":"SK Das","year":"2020","unstructured":"Das, S. K., Ghosh, G. K. & Avasthe, R. Evaluating biomas-derived biochar on seed germination and early seedling growth of maize and black gram. Biomass Convers. Biorefin. 12, 1\u201314 (2020).","journal-title":"Biomass Convers. Biorefin."},{"key":"93198_CR12","doi-asserted-by":"publisher","first-page":"116845","DOI":"10.1016\/j.geoderma.2024.116845","volume":"443","author":"X Wang","year":"2024","unstructured":"Wang, X. et al. Biochar addition reduces salinity in salt-affected soils with no impact on soil pH: A meta-analysis. Geoderma 443, 116845 (2024).","journal-title":"Geoderma"},{"key":"93198_CR13","doi-asserted-by":"publisher","first-page":"2214","DOI":"10.2134\/agronj2016.02.0100","volume":"108","author":"J Chang","year":"2016","unstructured":"Chang, J. et al. Corn stover biochar reduced N2O and CO2 emissions in soil with different water filled pore spaces and diurnal temperature cycles. Agron. J 108, 2214\u20132221 (2016).","journal-title":"Agron. J"},{"key":"93198_CR14","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1016\/j.jenvman.2018.11.074","volume":"232","author":"BSL Coleman","year":"2019","unstructured":"Coleman, B. S. L., Easton, Z. M. & Bock, E. M. Biochar fails to enhance nutrient removal in woodchip bioreactor columns following saturation. J. Environ. Manag. 232, 490\u2013498. https:\/\/doi.org\/10.1016\/j.jenvman.2018.11.074 (2019).","journal-title":"J. Environ. Manag."},{"key":"93198_CR15","doi-asserted-by":"publisher","first-page":"120448","DOI":"10.1016\/j.jenvman.2024.120448","volume":"355","author":"H Sultan","year":"2024","unstructured":"Sultan, H. et al. Biochar and nano biochar: Enhancing salt resilience in plants and soil while mitigating greenhouse gas emissions: A comprehensive review. J. Environ. Manag. 355, 120448 (2024).","journal-title":"J. Environ. Manag."},{"key":"93198_CR16","doi-asserted-by":"publisher","first-page":"215","DOI":"10.4324\/9781849770552","volume-title":"Biochar for Environmental Management","author":"J Lehmann","year":"2012","unstructured":"Lehmann, J., Czimczik, C., Laird, D. & Sohi, S. Biochar for Environmental Management 215\u2013238 (Routledge, 2012)."},{"key":"93198_CR17","doi-asserted-by":"publisher","first-page":"1206820","DOI":"10.3389\/fpls.2023.1206820","volume":"14","author":"K Huang","year":"2023","unstructured":"Huang, K. et al. Soil acidification and salinity: The importance of biochar application to agricultural soils. Front. Plant Sci. 14, 1206820 (2023).","journal-title":"Front. Plant Sci."},{"key":"93198_CR18","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.chemosphere.2015.06.041","volume":"142","author":"H-S Kim","year":"2016","unstructured":"Kim, H.-S. et al. Effect of biochar on reclaimed tidal land soil properties and maize (Zea mays L.) response. Chemosphere 142, 153\u2013159 (2016).","journal-title":"Chemosphere"},{"key":"93198_CR19","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1071\/FP15054","volume":"42","author":"SS Akhtar","year":"2015","unstructured":"Akhtar, S. S., Andersen, M. N., Naveed, M., Zahir, Z. A. & Liu, F. Interactive effect of biochar and plant growth-promoting bacterial endophytes on ameliorating salinity stress in maize. Funct. Plant Biol. 42, 770\u2013781 (2015).","journal-title":"Funct. Plant Biol."},{"key":"93198_CR20","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1007\/s00374-005-0858-3","volume":"41","author":"EJ Bateman","year":"2005","unstructured":"Bateman, E. J. & Baggs, E. M. Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space. Biol. Fertility Soils 41, 379\u2013388. https:\/\/doi.org\/10.1007\/s00374-005-0858-3 (2005).","journal-title":"Biol. Fertility Soils"},{"key":"93198_CR21","doi-asserted-by":"publisher","first-page":"1110151","DOI":"10.3389\/fmicb.2022.1110151","volume":"13","author":"H Wang","year":"2023","unstructured":"Wang, H. et al. Quantifying nitrous oxide production rates from nitrification and denitrification under various moisture conditions in agricultural soils: Laboratory study and literature synthesis. Front. Microbiol. 13, 1110151. https:\/\/doi.org\/10.3389\/fmicb.2022.1110151 (2023).","journal-title":"Front. Microbiol."},{"key":"93198_CR22","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.agee.2010.09.003","volume":"139","author":"A Zhang","year":"2010","unstructured":"Zhang, A. et al. Effect of biochar amendment on yield and methane and nitrous oxide emissions from a rice paddy from Tai Lake plain, China. Agric. Ecosyst. Environ. 139, 469\u2013475 (2010).","journal-title":"Agric. Ecosyst. Environ."},{"key":"93198_CR23","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.scitotenv.2013.04.014","volume":"465","author":"T Kennedy","year":"2013","unstructured":"Kennedy, T., Decock, C. & Six, J. Assessing drivers of N2O production in California tomato cropping systems. Sci. Total Environ. 465, 36\u201347 (2013).","journal-title":"Sci. Total Environ."},{"key":"93198_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41586-024-07464-3","volume":"630","author":"EG Hiis","year":"2024","unstructured":"Hiis, E. G. et al. Unlocking bacterial potential to reduce farmland N2O emissions. Nature 630, 1\u20138 (2024).","journal-title":"Nature"},{"key":"93198_CR25","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1002\/agj2.21340","volume":"115","author":"DR Joshi","year":"2023","unstructured":"Joshi, D. R. et al. A global meta-analysis of cover crop response on soil carbon storage within a corn production system. Agronomy J. 115, 1543\u20131556 (2023).","journal-title":"Agronomy J."},{"key":"93198_CR26","doi-asserted-by":"publisher","first-page":"A3","DOI":"10.1016\/j.soilbio.2018.03.020","volume":"123","author":"N Wrage-M\u00f6nnig","year":"2018","unstructured":"Wrage-M\u00f6nnig, N. et al. The role of nitrifier denitrification in the production of nitrous oxide revisited. Soil Biol. Biochem. 123, A3\u2013A16 (2018).","journal-title":"Soil Biol. Biochem."},{"key":"93198_CR27","doi-asserted-by":"publisher","first-page":"e112609","DOI":"10.1371\/journal.pone.0112609","volume":"9","author":"X Li","year":"2014","unstructured":"Li, X. et al. Functional potential of soil microbial communities in the maize rhizosphere. PLoS ONE 9, e112609 (2014).","journal-title":"PLoS ONE"},{"key":"93198_CR28","doi-asserted-by":"publisher","first-page":"e17507","DOI":"10.1111\/mec.17507","volume":"33","author":"Y Ming","year":"2024","unstructured":"Ming, Y. et al. Insights into the evolutionary and ecological adaption strategies of nirS-and nirK-type denitrifying communities. Mol. Ecology 33, e17507 (2024).","journal-title":"Mol. Ecology"},{"key":"93198_CR29","doi-asserted-by":"publisher","first-page":"381","DOI":"10.2489\/jswc.2022.00112","volume":"77","author":"D Fiedler","year":"2022","unstructured":"Fiedler, D. et al. Phytoremediation and high rainfall combine to improve soil and plant health in a North America Northern Great Plains saline sodic soil. J. Soil Water Conserv. 77, 381\u2013388 (2022).","journal-title":"J. Soil Water Conserv."},{"key":"93198_CR30","doi-asserted-by":"publisher","first-page":"e13132","DOI":"10.1111\/gcbb.13132","volume":"16","author":"L Cheng","year":"2024","unstructured":"Cheng, L. et al. Biochar produced at high temperature mitigates NO emission and promotes nitrogen retention in subtropical forest soils. GCB Bioenergy 16, e13132. https:\/\/doi.org\/10.1111\/gcbb.13132 (2024).","journal-title":"GCB Bioenergy"},{"key":"93198_CR31","doi-asserted-by":"publisher","first-page":"917","DOI":"10.3390\/f15060917","volume":"15","author":"N Sun","year":"2024","unstructured":"Sun, N. et al. Biochar addition increased soil carbon storage but did not exacerbate soil carbon emission in young subtropical plantation forest. Forests 15, 917 (2024).","journal-title":"Forests"},{"key":"93198_CR32","doi-asserted-by":"crossref","unstructured":"DeSutter, T. M., Clay, D. E., Oster, J., Westhoff, S. & Alvarez-Torres, B. Laboratory methods for determining salinity and sodicity. In Salinity and Sodicity: A Growing Global Challenge to Food Security, Environmental Quality and Soil Resilience (eds. Clay, D.E., DeSutter, T.M., Clay, S.A. & Nleya, T.)  31\u201340 (Wiley, 2024).","DOI":"10.1002\/9780891183976.ch4"},{"key":"93198_CR33","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1002\/agj2.21184","volume":"115","author":"SA Clay","year":"2023","unstructured":"Clay, S. A., Fiedler, D., Reese, C. & Clay, D. E. Restoring ecological function to saline\u2013sodic soils in South Dakota with perennial grass mixtures. Agronomy J. 115, 135\u2013146 (2023).","journal-title":"Agronomy J."},{"key":"93198_CR34","volume-title":"Evaluating Canola Genotypes for Growth and Yield Under Different Environmental Conditions in South Dakota","author":"U Arinaitwe","year":"2021","unstructured":"Arinaitwe, U. Evaluating Canola Genotypes for Growth and Yield Under Different Environmental Conditions in South Dakota (South Dakota State University, 2021)."},{"key":"93198_CR35","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1111\/jac.12132","volume":"201","author":"SS Akhtar","year":"2015","unstructured":"Akhtar, S. S., Andersen, M. N. & Liu, F. Biochar mitigates salinity stress in potato. J. Agronomy Crop Sci. 201, 368\u2013378 (2015).","journal-title":"J. Agronomy Crop Sci."},{"key":"93198_CR36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12870-019-2170-7","volume":"20","author":"Y Zhang","year":"2020","unstructured":"Zhang, Y., Ding, J., Wang, H., Su, L. & Zhao, C. Biochar addition alleviate the negative effects of drought and salinity stress on soybean productivity and water use efficiency. BMC Plant Biol. 20, 1\u201311 (2020).","journal-title":"BMC Plant Biol."},{"key":"93198_CR37","doi-asserted-by":"publisher","first-page":"106018","DOI":"10.1016\/j.catena.2022.106018","volume":"211","author":"X Lee","year":"2022","unstructured":"Lee, X. et al. Use of biochar to manage soil salts and water: Effects and mechanisms. Catena 211, 106018 (2022).","journal-title":"Catena"},{"key":"93198_CR38","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.sjbs.2021.09.035","volume":"29","author":"AA Rahi","year":"2022","unstructured":"Rahi, A. A. et al. Toxicity of Cadmium and nickel in the context of applied activated carbon biochar for improvement in soil fertility. Saudi J. Biol. Sci. 29, 743\u2013750 (2022).","journal-title":"Saudi J. Biol. Sci."},{"key":"93198_CR39","doi-asserted-by":"publisher","first-page":"17413","DOI":"10.1038\/s41598-021-96771-0","volume":"11","author":"K Dawar","year":"2021","unstructured":"Dawar, K. et al. Biochar and urease inhibitor mitigate NH3 and N2O emissions and improve wheat yield in a urea fertilized alkaline soil. Sci. Rep. 11, 17413 (2021).","journal-title":"Sci. Rep."},{"key":"93198_CR40","doi-asserted-by":"publisher","first-page":"471","DOI":"10.2489\/jswc.69.6.471","volume":"69","author":"AD Basche","year":"2014","unstructured":"Basche, A. D., Miguez, F. E., Kaspar, T. C. & Castellano, M. J. Do cover crops increase or decrease nitrous oxide emissions? A meta-analysis. J. Soil and Water Conserv. 69, 471\u2013482 (2014).","journal-title":"J. Soil and Water Conserv."},{"key":"93198_CR41","volume-title":"Recommended Chemical Soil Test Procedures for the North Central Region","author":"M Nathan","year":"2012","unstructured":"Nathan, M. & Gelderman, R. Recommended Chemical Soil Test Procedures for the North Central Region (Univ. of Missouri, 2012)."},{"key":"93198_CR42","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.1016\/j.ecolind.2019.02.012","volume":"101","author":"A Datta","year":"2019","unstructured":"Datta, A., Mandal, A. & Yadav, R. Proper measurement of electrical conductivity and other parameters influence profile salinity and sodicity under different land uses. Ecol. Indicators 101, 1004\u20131006 (2019).","journal-title":"Ecol. Indicators"},{"key":"93198_CR43","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.2136\/sssaj2015.01.0010n","volume":"79","author":"T DeSutter","year":"2015","unstructured":"DeSutter, T. et al. Relating sodium percentage to sodium adsorption ratio and its utility in the northern Great Plains. Soil Sci. Soc. Am. J. 79, 1261\u20131264 (2015).","journal-title":"Soil Sci. Soc. Am. J."},{"key":"93198_CR44","doi-asserted-by":"publisher","first-page":"e1569","DOI":"10.21769\/BioProtoc.1569","volume":"5","author":"LJ White","year":"2015","unstructured":"White, L. J., Br\u00f6zel, V. S. & Subramanian, S. Isolation of rhizosphere bacterial communities from soil. Bio-protocol 5, e1569\u2013e1569 (2015).","journal-title":"Bio-protocol"},{"key":"93198_CR45","doi-asserted-by":"publisher","first-page":"466","DOI":"10.2136\/sssaj2018.03.0105","volume":"83","author":"WR Roper","year":"2019","unstructured":"Roper, W. R., Robarge, W. P., Osmond, D. L. & Heitman, J. L. Comparing four methods of measuring soil organic matter in North Carolina soils. Soil Sci. Soc. Am. J. 83, 466\u2013474 (2019).","journal-title":"Soil Sci. Soc. Am. J."},{"key":"93198_CR46","doi-asserted-by":"publisher","first-page":"2096","DOI":"10.1128\/AEM.66.5.2096-2104.2000","volume":"66","author":"G Braker","year":"2000","unstructured":"Braker, G., Zhou, J., Wu, L., Devol, A. H. & Tiedje, J. M. Nitrite reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in Pacific Northwest marine sediment communities. Appl. Environ. Microbiol. 66, 2096\u20132104 (2000).","journal-title":"Appl. Environ. Microbiol."},{"key":"93198_CR47","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/B978-044452857-5.50006-0","volume-title":"Biology of the Nitrogen Cycle","author":"WG Zumft","year":"2007","unstructured":"Zumft, W. G. & K\u00f6rner, H. Biology of the Nitrogen Cycle 67\u201381 (Elsevier, 2007)."},{"key":"93198_CR48","doi-asserted-by":"publisher","first-page":"e00053","DOI":"10.1128\/CMR.00053-19","volume":"33","author":"DL Church","year":"2020","unstructured":"Church, D. L. et al. Performance and application of 16S rRNA gene cycle sequencing for routine identification of bacteria in the clinical microbiology laboratory. Clin. Microbiol. Rev. 33, e00053. https:\/\/doi.org\/10.1128\/CMR.00053-19 (2020).","journal-title":"Clin. Microbiol. Rev."},{"key":"93198_CR49","doi-asserted-by":"publisher","first-page":"9067","DOI":"10.1038\/s41598-024-59667-3","volume":"14","author":"O Barto\u0161","year":"2024","unstructured":"Barto\u0161, O., Chmel, M. & Swierczkov\u00e1, I. The overlooked evolutionary dynamics of 16S rRNA revises its role as the \u201cgold standard\u201d for bacterial species identification. Sci. Rep. 14, 9067 (2024).","journal-title":"Sci. Rep."},{"key":"93198_CR50","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1016\/j.biortech.2013.04.112","volume":"140","author":"G Angnes","year":"2013","unstructured":"Angnes, G. et al. Correlating denitrifying catabolic genes with N2O and N2 emissions from swine slurry composting. Bioresour. Technol. 140, 368\u2013375 (2013).","journal-title":"Bioresour. Technol."},{"key":"93198_CR51","doi-asserted-by":"publisher","first-page":"3476","DOI":"10.1128\/AEM.69.6.3476-3483.2003","volume":"69","author":"G Braker","year":"2003","unstructured":"Braker, G. & Tiedje, J. M. Nitric oxide reductase (norB) genes from pure cultures and environmental samples. Appl. Environ. Microbiol. 69, 3476\u20133483 (2003).","journal-title":"Appl. Environ. Microbiol."},{"key":"93198_CR52","doi-asserted-by":"publisher","first-page":"e01193","DOI":"10.1128\/mBio.01193-14","volume":"5","author":"L Orellana","year":"2014","unstructured":"Orellana, L. et al. Detecting nitrous oxide reductase (nosZ) genes in soil metagenomes: Method development and implications for the nitrogen cycle. MBio 5, e01193. https:\/\/doi.org\/10.1128\/mBio.01193-14 (2014).","journal-title":"MBio"},{"key":"93198_CR53","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.apsoil.2012.06.005","volume":"61","author":"JS Buyer","year":"2012","unstructured":"Buyer, J. S. & Sasser, M. High throughput phospholipid fatty acid analysis of soils. Appl. Soil Ecol. 61, 127\u2013130 (2012).","journal-title":"Appl. Soil Ecol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-93198-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-93198-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-93198-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T13:41:15Z","timestamp":1741959675000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-93198-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,14]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["93198"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-93198-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,14]]},"assertion":[{"value":"6 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"8879"}}