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The sensor successfully recognized vitamin B6 (Pyridoxine) in concentrations ranging from 2 to 10\u00a0\u00b5M by employing CdTe quantum dots (Cadmium Telluride QDs) on the ZnO thin film. This detection was achieved by implementing an amperometric (I\u2013V) method, typically operating at a working voltage below 2\u00a0V. From the slope of the calibration curve, the sensitivity, and the limit of detection values are estimated to be 7.56\u2009\u00b1\u20090.92\u00a0nA\/\u00b5M and 0.906\u00a0\u00b5M (n\u2009=\u20095), respectively. This study conducted electrochemical impedance spectroscopy (EIS) in conjunction with current\u2013voltage (I\u2013V) measurements. The results obtained from EIS are consistent with the current response observed in the I\u2013V measurements. The sensing mechanism primarily relies on the charge transfer phenomenon (electrostatic attraction) between the analyte (vitamin B6 mediated by CdTe quantum dots) and the ZnO thin film. In summary, our work introduces a simple, rapid, and cost-effective ZnO thin film-based sensing device for detecting vitamin B6, mediated by CdTe QDs. This sensor holds promise for a wide range of applications, from pharmaceutical quality control to point-of-care diagnostics and routine vitamin B screening.<\/jats:p>","DOI":"10.1038\/s41598-025-95892-0","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T10:40:01Z","timestamp":1743763201000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of low cost ZnO based chemi resistive biosensor for the detection of vitamin B6 mediated by quantum dots"],"prefix":"10.1038","volume":"15","author":[{"given":"Bhavana","family":"Anchan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saritha","family":"Kamath U","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh D.","family":"Kulkarni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavita A.","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shounak","family":"De","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajeetkumar","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"95892_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nfs.2018.03.001","author":"K Biesalski Hans","year":"2018","unstructured":"Biesalski Hans, K. & Jana, T. 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