{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:06:44Z","timestamp":1790082404712,"version":"4.0.1"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["BPD-00626-22 and APQ-01297-23"],"award-info":[{"award-number":["BPD-00626-22 and APQ-01297-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["BPD-00626-22 and APQ-01297-23"],"award-info":[{"award-number":["BPD-00626-22 and APQ-01297-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["BPD-00626-22 and APQ-01297-23"],"award-info":[{"award-number":["BPD-00626-22 and APQ-01297-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-96755-4","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T12:16:42Z","timestamp":1744373802000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Multivalent vaccine candidate from conserved immunogenic peptides in entry or exit proteins of Orthopoxvirus genus"],"prefix":"10.1038","volume":"15","author":[{"given":"Leonardo Pereira","family":"de Ara\u00fajo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evandro Neves","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Severino Matias","family":"de Alencar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Paiva","family":"Corsetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Augusto","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"issue":"5","key":"96755_CR1","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1038\/s12276-021-00608-9","volume":"53","author":"YK Choi","year":"2021","unstructured":"Choi, Y. K. Emerging and re-emerging fatal viral diseases. Exp. Mol. Med. 53 (5), 711\u2013712 (2021).","journal-title":"Exp. Mol. Med."},{"key":"96755_CR2","unstructured":"Mpox Vaccination from CDC. https:\/\/www.cdc.gov\/poxvirus\/mpox\/interim-considerations\/overview.html (Accessed 25 August 2024)."},{"issue":"10454","key":"96755_CR3","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1016\/S0140-6736(24)01751-3","volume":"404","author":"P Adepoju","year":"2024","unstructured":"Adepoju, P. Mpox declared a public health emergency. Lancet 404 (10454), e1\u2013e2 (2024).","journal-title":"Lancet"},{"issue":"2","key":"96755_CR4","doi-asserted-by":"publisher","first-page":"388","DOI":"10.3390\/v14020388","volume":"14","author":"IV Babkin","year":"2022","unstructured":"Babkin, I. V., Babkina, I. N. & Tikunova, N. V. An update of orthopoxvirus molecular evolution. Viruses 14 (2), 388 (2022).","journal-title":"Viruses"},{"key":"96755_CR5","doi-asserted-by":"publisher","first-page":"198975","DOI":"10.1016\/j.virusres.2022.198975","volume":"323","author":"C Molteni","year":"2023","unstructured":"Molteni, C. et al. Evolution of the orthopoxvirus core genome. Virus Res. 323, 198975 (2023).","journal-title":"Virus Res."},{"issue":"6502","key":"96755_CR6","doi-asserted-by":"publisher","first-page":"eaaw8977","DOI":"10.1126\/science.aaw8977","volume":"369","author":"B M\u00fchlemann","year":"2020","unstructured":"M\u00fchlemann, B. et al. Diverse variola virus (smallpox) strains were widespread in Northern Europe in the Viking age. Science 369 (6502), eaaw8977 (2020).","journal-title":"Science"},{"issue":"1","key":"96755_CR7","doi-asserted-by":"publisher","first-page":"103","DOI":"10.3390\/v15010103","volume":"15","author":"GA Shchelkunova","year":"2022","unstructured":"Shchelkunova, G. A. & Shchelkunov, S. N. Smallpox, Monkeypox and other human orthopoxvirus infections. Viruses 15 (1), 103 (2022).","journal-title":"Viruses"},{"issue":"4","key":"96755_CR8","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1016\/j.wem.2021.08.003","volume":"32","author":"JH Diaz","year":"2021","unstructured":"Diaz, J. H. The disease ecology, epidemiology, clinical manifestations, management, prevention, and control of increasing human infections with animal orthopoxviruses. Wilderness Environ. Med. 32 (4), 528\u2013536 (2021).","journal-title":"Wilderness Environ. Med."},{"issue":"5","key":"96755_CR9","doi-asserted-by":"publisher","first-page":"e282","DOI":"10.1016\/S1473-3099(24)00213-5","volume":"24","author":"S Devi","year":"2024","unstructured":"Devi, S. First fatality from Alaskapox virus. Lancet Infect. Dis. 24 (5), e282 (2024).","journal-title":"Lancet Infect. Dis."},{"issue":"2","key":"96755_CR10","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1001\/jama.2022.10259","volume":"328","author":"K Walter","year":"2022","unstructured":"Walter, K. & Malani, P. N. What is Monkeypox? JAMA 328 (2), 222 (2022).","journal-title":"JAMA"},{"issue":"2","key":"96755_CR11","doi-asserted-by":"publisher","first-page":"2223669","DOI":"10.1080\/22221751.2023.2223669","volume":"12","author":"M Li","year":"2023","unstructured":"Li, M. et al. Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus. Emerg. Microbes Infect. 12 (2), 2223669 (2023).","journal-title":"Emerg. Microbes Infect."},{"issue":"Supplement_2","key":"96755_CR12","doi-asserted-by":"publisher","first-page":"S285","DOI":"10.1093\/infdis\/jiad443","volume":"229","author":"LP De Ara\u00fajo","year":"2024","unstructured":"De Ara\u00fajo, L. P. et al. Immunoinformatic approach for rational identification of Immunogenic peptides against host entry and\/or exit Monkeypox proteins and potential multi-epitope vaccine construction. J. Infect. Dis. 229 (Supplement_2), S285\u2013S292 (2024).","journal-title":"J. Infect. Dis."},{"issue":"7","key":"96755_CR13","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1016\/S2666-5247(24)00095-8","volume":"5","author":"LP De Ara\u00fajo","year":"2024","unstructured":"De Ara\u00fajo, L. P. et al. Shared Immunogenic epitopes between host entry and exit proteins from Monkeypox and Alaskapox viruses. Lancet Microbe. 5 (7), 624\u2013625 (2024).","journal-title":"Lancet Microbe"},{"issue":"D1","key":"96755_CR14","doi-asserted-by":"publisher","first-page":"D482","DOI":"10.1093\/nar\/gkw1065","volume":"45","author":"EL Hatcher","year":"2017","unstructured":"Hatcher, E. L. et al. Virus variation resource\u2013improved response to emergent viral outbreaks. Nucleic Acids Res. 45 (D1), D482\u2013D490 (2017).","journal-title":"Nucleic Acids Res."},{"issue":"9","key":"96755_CR15","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","volume":"25","author":"AM Waterhouse","year":"2009","unstructured":"Waterhouse, A. M. et al. Jalview version 2\u2014a multiple sequence alignment editor and analysis workbench. Bioinformatics 25 (9), 1189\u20131191 (2009).","journal-title":"Bioinformatics"},{"issue":"D1","key":"96755_CR16","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1093\/nar\/gky1006","volume":"47","author":"R Vita","year":"2019","unstructured":"Vita, R. et al. The immune epitope database (IEDB): 2018 update. Nucleic Acids Res. 47 (D1), 339\u2013343 (2019).","journal-title":"Nucleic Acids Res."},{"key":"96755_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-8-4","volume":"8","author":"IA Doytchinova","year":"2007","unstructured":"Doytchinova, I. A. & Flower, D. R. VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinf. 8, 1\u20137 (2007).","journal-title":"BMC Bioinf."},{"key":"96755_CR18","doi-asserted-by":"crossref","unstructured":"Nguyen, M. N. et al. AllerCatPro 2.0: a web server for predicting protein allergenicity potential. Nucleic Acids Res. 50 (W1), W36\u2013W43 .","DOI":"10.1093\/nar\/gkac446"},{"issue":"4","key":"96755_CR19","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1093\/bioinformatics\/14.4.378","volume":"14","author":"T Hirokawa","year":"1998","unstructured":"Hirokawa, T., Boon-Chieng, S. & Mitaku, S. SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 14 (4), 378\u2013379 (1998).","journal-title":"Bioinformatics"},{"key":"96755_CR20","unstructured":"Gupta, R. & Brunak, S. Prediction of glycosylation across the human proteome and the correlation to protein function. In Pac. Symp. Biocomput. 310\u2013322. (2002)."},{"issue":"1","key":"96755_CR21","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1093\/bioinformatics\/btaa1102","volume":"37","author":"J Hon","year":"2021","unstructured":"Hon, J. et al. SoluProt: prediction of soluble protein expression in Escherichia coli. Bioinformatics 37 (1), 23\u201328 (2021).","journal-title":"Bioinformatics"},{"issue":"4","key":"96755_CR22","doi-asserted-by":"publisher","first-page":"e9862","DOI":"10.1371\/journal.pone.0009862","volume":"5","author":"N Rapin","year":"2010","unstructured":"Rapin, N. et al. Computational immunology Meets bioinformatics: the use of prediction tools for molecular binding in the simulation of the immune system. PLoS ONE 5 (4), e9862 (2010).","journal-title":"PLoS ONE"},{"issue":"8016","key":"96755_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41586-024-07487-w","volume":"630","author":"J Abramson","year":"2024","unstructured":"Abramson, J. et al. Accurate structure prediction of biomolecular interactions with alphafold 3. Nature 630 (8016), 1\u20133 (2024).","journal-title":"Nature"},{"key":"96755_CR24","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","volume":"1","author":"MJ Abraham","year":"2015","unstructured":"Abraham, M. J. et al. High performance molecular simulations through multi-level parallelism from laptops to supercomputers. SoftwareX 1, 19\u201325 (2015).","journal-title":"SoftwareX"},{"issue":"11","key":"96755_CR25","doi-asserted-by":"publisher","first-page":"2400106","DOI":"10.2807\/1560-7917.ES.2024.29.11.2400106","volume":"29","author":"LM Masirika","year":"2024","unstructured":"Masirika, L. M. et al. Ongoing Mpox outbreak in Kamituga, South Kivu Province, associated with Monkeypox virus of a novel clade I sub-lineage, Democratic Republic of the congo, 2024. Euro Surveill. 29 (11), 2400106 (2024).","journal-title":"Euro Surveill."},{"issue":"1","key":"96755_CR26","doi-asserted-by":"publisher","first-page":"2600","DOI":"10.1080\/22221751.2022.2132882","volume":"11","author":"MM Hatmal","year":"2022","unstructured":"Hatmal, M. M. et al. Comprehensive literature review of Monkeypox. Emerg. Microbes Infect. 11 (1), 2600\u20132631 (2022).","journal-title":"Emerg. Microbes Infect."},{"issue":"D1","key":"96755_CR27","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1093\/nar\/gkac1096","volume":"51","author":"J Wang","year":"2023","unstructured":"Wang, J. et al. The conserved domain database in 2023. Nucleic Acids Res. 51 (D1), 384\u2013388 (2023).","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"96755_CR28","doi-asserted-by":"publisher","first-page":"6496","DOI":"10.1038\/s41598-022-10230-y","volume":"12","author":"ES Geanes","year":"2022","unstructured":"Geanes, E. S. et al. Cross-reactive antibodies elicited to conserved epitopes on SARS-CoV-2 Spike protein after infection and vaccination. Sci. Rep. 12 (1), 6496 (2022).","journal-title":"Sci. Rep."},{"issue":"75","key":"96755_CR29","doi-asserted-by":"publisher","first-page":"2427","DOI":"10.1126\/sciimmunol.abq2427","volume":"7","author":"J Quandt","year":"2022","unstructured":"Quandt, J. et al. Omicron BA. 1 breakthrough infection drives cross-variant neutralization and memory B cell formation against conserved epitopes. Sci. Immunol. 7 (75), 2427 (2022).","journal-title":"Sci. Immunol."},{"issue":"24","key":"96755_CR30","doi-asserted-by":"publisher","first-page":"e2220294120","DOI":"10.1073\/pnas.2220294120","volume":"120","author":"AI Mosa","year":"2023","unstructured":"Mosa, A. I. et al. Polyvalent immunization elicits a synergistic broadly neutralizing immune response to hypervariable region 1 variants of hepatitis C virus. Proc. Natl. Acad. Sci. 120 (24), e2220294120 (2023).","journal-title":"Proc. Natl. Acad. Sci."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96755-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96755-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96755-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T12:16:45Z","timestamp":1744373805000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96755-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,11]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["96755"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-96755-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,11]]},"assertion":[{"value":"18 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"12503"}}