{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T04:14:35Z","timestamp":1788754475637,"version":"build-2803163510"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-96783-0","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T16:19:55Z","timestamp":1745338795000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Biocatalytic production of 3-hydroxypropionic acid precursors using a regioselective Baeyer-Villiger monooxygenase"],"prefix":"10.1038","volume":"15","author":[{"given":"Melissa","family":"De Angelis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Correddu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Bucciol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Tabasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Catucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Cravotto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Roggero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Gilardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila J.","family":"Sadeghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"96783_CR1","doi-asserted-by":"publisher","unstructured":"Brink, G. J., Arends, I. W. C. E. & Sheldon, R. A. The Baeyer\u2009\u2013\u2009Villiger reaction: new developments toward greener procedures. Chem. Rev. 104 https:\/\/doi.org\/10.1021\/cr030011l (2004).","DOI":"10.1021\/cr030011l"},{"key":"96783_CR2","doi-asserted-by":"publisher","unstructured":"Fatima, S. et al. Baeyer\u2013Villiger oxidation: a promising tool for the synthesis of natural products: a review. RSC Adv. 14 https:\/\/doi.org\/10.1039\/D4RA03914A (2024).","DOI":"10.1039\/D4RA03914A"},{"key":"96783_CR3","doi-asserted-by":"publisher","unstructured":"Aslan, A. S. et al. Semi-Rational design of Geobacillus stearothermophilus L-Lactate dehydrogenase to access various chiral \u03b1-Hydroxy acids. Appl. Biochem. Biotechnol. 179 https:\/\/doi.org\/10.1007\/s12010-016-2007-x (2016).","DOI":"10.1007\/s12010-016-2007-x"},{"key":"96783_CR4","doi-asserted-by":"publisher","unstructured":"Rowles, I. et al. Engineering of phenylalanine ammonia lyase from Rhodotorula Graminis for the enhanced synthesis of unnatural l-amino acids. Tetrahedron 72 https:\/\/doi.org\/10.1016\/j.tet.2016.06.026 (2016).","DOI":"10.1016\/j.tet.2016.06.026"},{"key":"96783_CR5","doi-asserted-by":"publisher","unstructured":"F\u00fcrst, M. J. L. J., Gran-Scheuch, A., Aalbers, F. S. & Fraaije, M. W. Baeyer\u2013Villiger monooxygenases: tunable oxidative biocatalysts. ACS Catal. 9 https:\/\/doi.org\/10.1021\/acscatal.9b03396 (2019).","DOI":"10.1021\/acscatal.9b03396"},{"issue":"8","key":"96783_CR6","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/s00253-021-11230-0","volume":"105","author":"F Liu","year":"2021","unstructured":"Liu, F. et al. A Baeyer-Villiger monooxygenase from Cupriavidus basilensis catalyzes asymmetric synthesis of (R)-lansoprazole and other pharmaco-sulfoxides. Appl. Microbiol. Biotechnol. 105 (8), 105. https:\/\/doi.org\/10.1007\/s00253-021-11230-0 (2021). 2021-03-29.","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"96783_CR7","doi-asserted-by":"publisher","first-page":"07","DOI":"10.3390\/catal11080932","volume":"11","author":"D Schwendenwein","year":"2021","unstructured":"Schwendenwein, D. et al. Chemo-Enzymatic cascade for the generation of fragrance aldehydes. Catalysts 2021. 11, 07\u201330. https:\/\/doi.org\/10.3390\/catal11080932 (2021).","journal-title":"Catalysts 2021"},{"key":"96783_CR8","doi-asserted-by":"crossref","unstructured":"Bu\u010dko, M. et al. Baeyer-Villiger oxidations: biotechnological approach. Applied Microbiology and Biotechnology 100 (2016). (2016-06-21). https:\/\/doi.org\/10.1007\/s00253-016-7670-x.","DOI":"10.1007\/s00253-016-7670-x"},{"key":"96783_CR9","doi-asserted-by":"publisher","unstructured":"Ceccoli, R. D. et al. Sequential chemo\u2013biocatalytic synthesis of aroma compounds. Green Chem. 25, https:\/\/doi.org\/10.1039\/D2GC02866B (2023).","DOI":"10.1039\/D2GC02866B"},{"key":"96783_CR10","doi-asserted-by":"publisher","unstructured":"Zhang, G. X. et al. Discovery and Engineering of a Novel Baeyer-Villiger Monooxygenase with High Normal Regioselectivity. ChemBioChem 22 (2021). \/04\/06 https:\/\/doi.org\/10.1002\/cbic.202000478","DOI":"10.1002\/cbic.202000478"},{"key":"96783_CR11","doi-asserted-by":"publisher","unstructured":"Werpy, T. & Petersen, G. Top Value Added Chemicals from Biomass: I -- Results of Screening for Potential Candidates from Sugars and Synthesis Gas. (2004). https:\/\/doi.org\/10.2172\/15008859","DOI":"10.2172\/15008859"},{"key":"96783_CR12","doi-asserted-by":"publisher","unstructured":"Della Pina, C., Falletta, E. & Rossi, M. A green approach to chemical Building blocks. The case of 3-hydroxypropanoic acid. Green Chem. 13 https:\/\/doi.org\/10.1039\/C1GC15052A (2011).","DOI":"10.1039\/C1GC15052A"},{"key":"96783_CR13","doi-asserted-by":"publisher","unstructured":"Zhang, Y. et al. Notable improvement of 3-Hydroxypropionic acid and 1,3-Propanediol coproduction using modular coculture engineering and pathway rebalancing. ACS Sustain. Chem. Eng. 9 https:\/\/doi.org\/10.1021\/acssuschemeng.1c00229 (2021).","DOI":"10.1021\/acssuschemeng.1c00229"},{"key":"96783_CR14","doi-asserted-by":"publisher","unstructured":"Bhagwat, S. S. et al. Sustainable production of acrylic acid via 3-Hydroxypropionic acid from lignocellulosic biomass. ACS Sustain. Chem. Eng. 9 https:\/\/doi.org\/10.1021\/acssuschemeng.1c05441 (2021).","DOI":"10.1021\/acssuschemeng.1c05441"},{"key":"96783_CR15","doi-asserted-by":"publisher","unstructured":"Kildegaard, K. R., Wang, Z., Chen, Y., Nielsen, J. & Borodina, I. Production of 3-hydroxypropionic acid from glucose and xylose by metabolically engineered Saccharomyces cerevisiae. Metabolic Eng. Commun. 2 https:\/\/doi.org\/10.1016\/j.meteno.2015.10.001 (2015).","DOI":"10.1016\/j.meteno.2015.10.001"},{"key":"96783_CR16","doi-asserted-by":"publisher","unstructured":"Oliveira, D. M., Mota, T. R., Ferrarese-Filho, O. & Santos, W. D. d. Sustainable production of succinic acid and 3-hydroxypropionic acid from renewable feedstocks. Production of Top 12 Biochemicals Selected by USDOE from Renewable Resources (2022). https:\/\/doi.org\/10.1016\/B978-0-12-823531-7.00008-1","DOI":"10.1016\/B978-0-12-823531-7.00008-1"},{"key":"96783_CR17","doi-asserted-by":"publisher","unstructured":"Rathnasingh, C. et al. Production of 3-hydroxypropionic acid via malonyl-CoA pathway using Recombinant Escherichia coli strains. J. Biotechnol. 157 https:\/\/doi.org\/10.1016\/j.jbiotec.2011.06.008 (2012).","DOI":"10.1016\/j.jbiotec.2011.06.008"},{"key":"96783_CR18","doi-asserted-by":"publisher","unstructured":"Liang, B. et al. Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3-hydroxypropionic acid. Biotechnol. Bioeng. 119 https:\/\/doi.org\/10.1002\/bit.28170 (2022). \/10\/01).","DOI":"10.1002\/bit.28170"},{"key":"96783_CR19","doi-asserted-by":"publisher","unstructured":"Di Bucchianico, M. Production of levulinic acid and alkyl levulinates: a process insight. Green Chem. 24 https:\/\/doi.org\/10.1039\/D1GC02457D (2022).","DOI":"10.1039\/D1GC02457D"},{"key":"96783_CR20","doi-asserted-by":"publisher","unstructured":"Wang, M., Peng, L., Gao, X., He, L. & Zhang, J. Efficient one-pot synthesis of alkyl levulinate from xylose with an integrated dehydration\/transfer-hydrogenation\/alcoholysis process. Sustainable Energy Fuels. 4 https:\/\/doi.org\/10.1039\/C9SE00982E (2020).","DOI":"10.1039\/C9SE00982E"},{"key":"96783_CR21","doi-asserted-by":"publisher","unstructured":"Fink, M. J. & Mihovilovic, M. D. Non-hazardous Baeyer\u2013Villiger oxidation of levulinic acid derivatives: alternative renewable access to 3-hydroxypropionates. Chem. Commun. 51 https:\/\/doi.org\/10.1039\/C4CC08734H (2015).","DOI":"10.1039\/C4CC08734H"},{"key":"96783_CR22","doi-asserted-by":"publisher","unstructured":"Liu, Y. Y., Li, C. X., Xu, J. H. & Zheng, G. W. Efficient synthesis of Methyl 3-Acetoxypropionate by a newly identified Baeyer-Villiger monooxygenase. Appl. Environ. Microbiol. 85 https:\/\/doi.org\/10.1128\/AEM.00239-19 (2019).","DOI":"10.1128\/AEM.00239-19"},{"key":"96783_CR23","doi-asserted-by":"publisher","unstructured":"Catucci, G. et al. Characterization of a new Baeyer-Villiger monooxygenase and conversion to a solely N-or S-oxidizing enzyme by a single R292 mutation. Biochim. Et Biophys. Acta (BBA) - Proteins Proteom. 1864 https:\/\/doi.org\/10.1016\/j.bbapap.2016.06.010 (2016).","DOI":"10.1016\/j.bbapap.2016.06.010"},{"key":"96783_CR24","doi-asserted-by":"publisher","unstructured":"L\u00f6bs, A. K., Lin, J. L., Cook, M. & Wheeldon, I. High throughput, colorimetric screening of microbial ester biosynthesis reveals high Ethyl acetate production from Kluyveromyces Marxianus on C5, C6, and C12 carbon sources. Biotechnol. J. 11 https:\/\/doi.org\/10.1002\/biot.201600060 (2016).","DOI":"10.1002\/biot.201600060"},{"key":"96783_CR25","doi-asserted-by":"publisher","unstructured":"Leisch, H., Morley, K. & Lau, P. C. K. Baeyer\u2009\u2013\u2009Villiger monooxygenases: more than just green chemistry. Chem. Rev. 111 (May 4). https:\/\/doi.org\/10.1021\/cr1003437 (2011).","DOI":"10.1021\/cr1003437"},{"key":"96783_CR26","doi-asserted-by":"publisher","unstructured":"Wu, H. et al. Methods for the regeneration of nicotinamide coenzymes. Green Chem. 15 https:\/\/doi.org\/10.1039\/C3GC37129H (2013).","DOI":"10.1039\/C3GC37129H"},{"key":"96783_CR27","doi-asserted-by":"publisher","unstructured":"Oeggl, R. et al. Frontiers | citrate as Cost-Efficient NADPH regenerating agent. Front. Bioeng. Biotechnol. 6 https:\/\/doi.org\/10.3389\/fbioe.2018.00196 (2018).","DOI":"10.3389\/fbioe.2018.00196"},{"key":"96783_CR28","doi-asserted-by":"publisher","unstructured":"Catucci, G. et al. Green production of Indigo and indirubin by an engineered Baeyer\u2013Villiger monooxygenase. Biocatal. Agric. Biotechnol. 44 https:\/\/doi.org\/10.1016\/j.bcab.2022.102458 (2022).","DOI":"10.1016\/j.bcab.2022.102458"},{"key":"96783_CR29","doi-asserted-by":"publisher","unstructured":"Catucci, G. et al. Production of drug metabolites by human FMO3 in Escherichia coli. Microbial Cell Factories 2020 19:1 19 (2020). https:\/\/doi.org\/10.1186\/s12934-020-01332-1","DOI":"10.1186\/s12934-020-01332-1"},{"key":"96783_CR30","doi-asserted-by":"publisher","unstructured":"Hanlon, S. P. et al. Expression of Recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale. Chem. Commun. 48 https:\/\/doi.org\/10.1039\/C2CC17878H (2012).","DOI":"10.1039\/C2CC17878H"},{"key":"96783_CR31","doi-asserted-by":"publisher","unstructured":"Li, J. Y. et al. Effect of Tween Series on Growth and cis-9, trans-11 Conjugated Linoleic Acid Production of Lactobacillus acidophilus F0221 in the Presence of Bile Salts. International Journal of Molecular Sciences 2011, Vol. 12, Pages 9138\u20139154 12 (2011). https:\/\/doi.org\/10.3390\/ijms12129138","DOI":"10.3390\/ijms12129138"},{"key":"96783_CR32","doi-asserted-by":"publisher","unstructured":"Wang, G. L. et al. Triton X-100 improves co-production of \u03b2-1,3-D-glucan and pullulan by Aureobasidium pullulans. Applied Microbiology and Biotechnology 2020 104:24 104 (2020). https:\/\/doi.org\/10.1007\/s00253-020-10992-3","DOI":"10.1007\/s00253-020-10992-3"},{"key":"96783_CR33","doi-asserted-by":"publisher","unstructured":"Wang, L. et al. Efficient biosynthesis of 10-Hydroxy-2-decenoic acid using a NAD(P)H regeneration P450 system and Whole-Cell catalytic biosynthesis. ACS Omega. 7 https:\/\/doi.org\/10.1021\/acsomega.2c00972 (2022).","DOI":"10.1021\/acsomega.2c00972"},{"key":"96783_CR34","doi-asserted-by":"publisher","unstructured":"Huang, Y. B., Yang, T., Cai, B., Chang, X. & Pan, H. Highly efficient metal salt catalyst for the esterification of biomass derived levulinic acid under microwave irradiation. RSC Adv. 6 https:\/\/doi.org\/10.1039\/C5RA24305J (2015).","DOI":"10.1039\/C5RA24305J"},{"key":"96783_CR35","doi-asserted-by":"publisher","unstructured":"Szab\u00f3, Y. et al. Microwave-induced base-catalyzed synthesis of Methyl levulinate, a further improvement in dimethyl carbonate-mediated valorization of levulinic acid. Appl. Catal. A. 651 https:\/\/doi.org\/10.1016\/j.apcata.2022.119020 (2023).","DOI":"10.1016\/j.apcata.2022.119020"},{"key":"96783_CR36","doi-asserted-by":"publisher","DOI":"10.3390\/ECCS2020-07549","author":"C Antonetti","year":"2020","unstructured":"Antonetti, C. et al. Production of levulinic acid and n-Butyl levulinate from the waste biomasses grape pomace and Cynara cardunculus L. Proc. 1st Int. Electron. Conf. Catal. Sci. https:\/\/doi.org\/10.3390\/ECCS2020-07549 (2020).","journal-title":"Proc. 1st Int. Electron. Conf. Catal. Sci."},{"key":"96783_CR37","doi-asserted-by":"publisher","unstructured":"Lorente, A. et al. Microwave radiation-assisted synthesis of levulinic acid from microcrystalline cellulose: application to a melon rind residue. Int. J. Biol. Macromol. 237 https:\/\/doi.org\/10.1016\/j.ijbiomac.2023.124149 (2023).","DOI":"10.1016\/j.ijbiomac.2023.124149"},{"key":"96783_CR38","doi-asserted-by":"publisher","unstructured":"Raspolli Galletti, A. M. et al. Insights on Butyl levulinate bio-blendstock: from model sugars to paper mill waste cellulose as feedstocks for a sustainable catalytic butanolysis process. Catal. Today. 418 https:\/\/doi.org\/10.1016\/j.cattod.2023.114054 (2023).","DOI":"10.1016\/j.cattod.2023.114054"},{"key":"96783_CR39","doi-asserted-by":"publisher","unstructured":"Minerdi, D., Zgrablic, I., Sadeghi, S. J. & Gilardi, G. Identification of a novel Baeyer-Villiger monooxygenase from acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA. Microb. Biotechnol. 5 https:\/\/doi.org\/10.1111\/j.1751-7915.2012.00356.x (2012).","DOI":"10.1111\/j.1751-7915.2012.00356.x"},{"key":"96783_CR40","doi-asserted-by":"publisher","unstructured":"Pence, H. E., Williams, A. & ChemSpider An online chemical information resource. J. Chem. Educ. 87 https:\/\/doi.org\/10.1021\/ed100697w (2010).","DOI":"10.1021\/ed100697w"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96783-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96783-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96783-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T16:19:56Z","timestamp":1745338796000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-96783-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,22]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["96783"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-96783-0","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,22]]},"assertion":[{"value":"17 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13986"}}