{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:11:47Z","timestamp":1789495907409,"version":"build-2803163510"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Mahidol University (Fundamental Fund: fiscal year 2023 by National Science Research and Innovation Fund"},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Faculty of Medical Technology, Mahidol University"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-97424-2","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T02:40:39Z","timestamp":1744684839000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Inhibitory effects of benzyl isothiocyanate on widespread mcr-1-harbouring IncX4 plasmid transfer"],"prefix":"10.1038","volume":"15","author":[{"given":"Sirirat","family":"Luk-In","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamonrat","family":"Phopin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasina","family":"Bangmuangngam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanittha","family":"Chatsuwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhammika Leshan","family":"Wannigama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aye Mya Sithu","family":"Shein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongpong","family":"Plongla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratana","family":"Lawung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakda","family":"Yainoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warawan","family":"Eiamphungporn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chawalit","family":"Chatupheeraphat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanawut","family":"Tantimongcolwat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"97424_CR1","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1016\/j.tim.2023.10.006","volume":"32","author":"JH Liu","year":"2024","unstructured":"Liu, J. H. et al. Plasmid-mediated colistin-resistance genes: mcr. Trends Microbiol 32, 365\u2013378. https:\/\/doi.org\/10.1016\/j.tim.2023.10.006 (2024).","journal-title":"Trends Microbiol"},{"key":"97424_CR2","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1038\/s41467-018-03205-z","volume":"9","author":"R Wang","year":"2018","unstructured":"Wang, R. et al. The global distribution and spread of the mobilized colistin resistance gene mcr-1. Nat Commun 9, 1179. https:\/\/doi.org\/10.1038\/s41467-018-03205-z (2018).","journal-title":"Nat Commun"},{"key":"97424_CR3","doi-asserted-by":"publisher","first-page":"2066","DOI":"10.1093\/jac\/dkw274","volume":"71","author":"S Schwarz","year":"2016","unstructured":"Schwarz, S. & Johnson, A. P. Transferable resistance to colistin: A new but old threat. J Antimicrob Chemother 71, 2066\u20132070. https:\/\/doi.org\/10.1093\/jac\/dkw274 (2016).","journal-title":"J Antimicrob Chemother"},{"key":"97424_CR4","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/S1473-3099(15)00424-7","volume":"16","author":"YY Liu","year":"2016","unstructured":"Liu, Y. Y. et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: A microbiological and molecular biological study. Lancet Infect Dis 16, 161\u2013168. https:\/\/doi.org\/10.1016\/S1473-3099(15)00424-7 (2016).","journal-title":"Lancet Infect Dis"},{"key":"97424_CR5","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S1473-3099(16)00007-4","volume":"16","author":"M Haenni","year":"2016","unstructured":"Haenni, M. et al. Co-occurrence of extended spectrum beta lactamase and MCR-1 encoding genes on plasmids. Lancet Infect Dis 16, 281\u2013282. https:\/\/doi.org\/10.1016\/S1473-3099(16)00007-4 (2016).","journal-title":"Lancet Infect Dis"},{"key":"97424_CR6","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1016\/S1473-3099(16)00009-8","volume":"16","author":"L Falgenhauer","year":"2016","unstructured":"Falgenhauer, L. et al. Colistin resistance gene mcr-1 in extended-spectrum beta-lactamase-producing and carbapenemase-producing Gram-negative bacteria in Germany. Lancet Infect Dis 16, 282\u2013283. https:\/\/doi.org\/10.1016\/S1473-3099(16)00009-8 (2016).","journal-title":"Lancet Infect Dis"},{"key":"97424_CR7","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1186\/s12879-022-07678-8","volume":"22","author":"S Ruekit","year":"2022","unstructured":"Ruekit, S. et al. Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017\u20132018). BMC Infect Dis 22, 695. https:\/\/doi.org\/10.1186\/s12879-022-07678-8 (2022).","journal-title":"BMC Infect Dis"},{"key":"97424_CR8","doi-asserted-by":"publisher","unstructured":"Karampatakis, T., Tsergouli, K. & Behzadi, P. Carbapenem-resistant Klebsiella pneumoniae: virulence factors, molecular epidemiology and latest updates in treatment options. Antibiotics (Basel) 12, https:\/\/doi.org\/10.3390\/antibiotics12020234 (2023).","DOI":"10.3390\/antibiotics12020234"},{"key":"97424_CR9","doi-asserted-by":"publisher","first-page":"1135614","DOI":"10.3389\/fmicb.2023.1135614","volume":"14","author":"A Algammal","year":"2023","unstructured":"Algammal, A., Hetta, H. F., Mabrok, M. & Behzadi, P. Editorial: Emerging multidrug-resistant bacterial pathogens \u201csuperbugs\u201d: A rising public health threat. Front Microbiol 14, 1135614. https:\/\/doi.org\/10.3389\/fmicb.2023.1135614 (2023).","journal-title":"Front Microbiol"},{"key":"97424_CR10","doi-asserted-by":"publisher","unstructured":"Murugaiyan, J. et al. Progress in alternative strategies to combat antimicrobial resistance: Focus on antibiotics. Antibiotics (Basel) 11, https:\/\/doi.org\/10.3390\/antibiotics11020200 (2022).","DOI":"10.3390\/antibiotics11020200"},{"key":"97424_CR11","doi-asserted-by":"publisher","first-page":"1756","DOI":"10.1093\/jac\/dkaa115","volume":"75","author":"RY Sun","year":"2020","unstructured":"Sun, R. Y. et al. Global clonal spread of mcr-3-carrying MDR ST34 Salmonella enterica serotype Typhimurium and monophasic 1,4,[5],12:i:- variants from clinical isolates. J Antimicrob Chemother 75, 1756\u20131765. https:\/\/doi.org\/10.1093\/jac\/dkaa115 (2020).","journal-title":"J Antimicrob Chemother"},{"key":"97424_CR12","doi-asserted-by":"publisher","unstructured":"Fukuda, A. et al. Co-harboring of cephalosporin (bla)\/colistin (mcr) resistance genes among Enterobacteriaceae from flies in Thailand. FEMS Microbiol. Lett. 365, https:\/\/doi.org\/10.1093\/femsle\/fny178 (2018).","DOI":"10.1093\/femsle\/fny178"},{"key":"97424_CR13","doi-asserted-by":"publisher","first-page":"14170","DOI":"10.1038\/s41598-021-93529-6","volume":"11","author":"S Luk-In","year":"2021","unstructured":"Luk-In, S. et al. Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand. Sci Rep 11, 14170. https:\/\/doi.org\/10.1038\/s41598-021-93529-6 (2021).","journal-title":"Sci Rep"},{"key":"97424_CR14","doi-asserted-by":"publisher","first-page":"109072","DOI":"10.1016\/j.isci.2024.109072","volume":"27","author":"KM Lagerstrom","year":"2024","unstructured":"Lagerstrom, K. M., Scales, N. C. & Hadly, E. A. Impressive pan-genomic diversity of E. coli from a wild animal community near urban development reflects human impacts. iScience 27, 109072. https:\/\/doi.org\/10.1016\/j.isci.2024.109072 (2024).","journal-title":"iScience"},{"key":"97424_CR15","doi-asserted-by":"publisher","first-page":"3517","DOI":"10.1099\/mic.0.28216-0","volume":"151","author":"R Fernandez-Lopez","year":"2005","unstructured":"Fernandez-Lopez, R. et al. Unsaturated fatty acids are inhibitors of bacterial conjugation. Microbiology 151, 3517\u20133526. https:\/\/doi.org\/10.1099\/mic.0.28216-0 (2005).","journal-title":"Microbiology"},{"key":"97424_CR16","doi-asserted-by":"publisher","first-page":"e01032","DOI":"10.1128\/mBio.01032-15","volume":"6","author":"M Getino","year":"2015","unstructured":"Getino, M. et al. Synthetic fatty acids prevent plasmid-mediated horizontal gene transfer. MBio 6, e01032-e11015. https:\/\/doi.org\/10.1128\/mBio.01032-15 (2015).","journal-title":"MBio"},{"key":"97424_CR17","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.ijantimicag.2019.01.011","volume":"53","author":"AA Kwapong","year":"2019","unstructured":"Kwapong, A. A., Stapleton, P. & Gibbons, S. Inhibiting plasmid mobility: The effect of isothiocyanates on bacterial conjugation. Int J Antimicrob Agents 53, 629\u2013636. https:\/\/doi.org\/10.1016\/j.ijantimicag.2019.01.011 (2019).","journal-title":"Int J Antimicrob Agents"},{"key":"97424_CR18","doi-asserted-by":"publisher","unstructured":"Elbediwi, M. et al. Global burden of colistin-resistant bacteria: mobilized colistin resistance genes study (1980\u20132018). Microorganisms 7, https:\/\/doi.org\/10.3390\/microorganisms7100461 (2019).","DOI":"10.3390\/microorganisms7100461"},{"key":"97424_CR19","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1186\/s12879-023-08539-8","volume":"23","author":"A Boonyasiri","year":"2023","unstructured":"Boonyasiri, A. et al. Characteristics and genomic epidemiology of colistin-resistant Enterobacterales from farmers, swine, and hospitalized patients in Thailand, 2014\u20132017. BMC Infect Dis 23, 556. https:\/\/doi.org\/10.1186\/s12879-023-08539-8 (2023).","journal-title":"BMC Infect Dis"},{"key":"97424_CR20","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/s41598-017-00095-x","volume":"7","author":"J Sun","year":"2017","unstructured":"Sun, J. et al. Genetic analysis of the IncX4 plasmids: Implications for a unique pattern in the mcr-1 acquisition. Sci Rep 7, 424. https:\/\/doi.org\/10.1038\/s41598-017-00095-x (2017).","journal-title":"Sci Rep"},{"key":"97424_CR21","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1093\/jac\/dkad097","volume":"78","author":"T Leangapichart","year":"2023","unstructured":"Leangapichart, T. et al. Exploring the epidemiology of mcr genes, genetic context and plasmids in Enterobacteriaceae originating from pigs and humans on farms in Thailand. J Antimicrob Chemother 78, 1395\u20131405. https:\/\/doi.org\/10.1093\/jac\/dkad097 (2023).","journal-title":"J Antimicrob Chemother"},{"key":"97424_CR22","doi-asserted-by":"publisher","first-page":"1450","DOI":"10.1038\/s41564-019-0445-2","volume":"4","author":"T He","year":"2019","unstructured":"He, T. et al. Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans. Nat Microbiol 4, 1450\u20131456. https:\/\/doi.org\/10.1038\/s41564-019-0445-2 (2019).","journal-title":"Nat Microbiol"},{"key":"97424_CR23","doi-asserted-by":"publisher","unstructured":"Zhang, R. et al. Chromosomal and plasmid-borne tigecycline resistance Genes tet(X3) and tet(X4) in dairy cows on a Chinese Farm. Antimicrob Agents Chemother 64. https:\/\/doi.org\/10.1128\/AAC.00674-20 (2020).","DOI":"10.1128\/AAC.00674-20"},{"key":"97424_CR24","doi-asserted-by":"publisher","first-page":"150687","DOI":"10.1016\/j.scitotenv.2021.150687","volume":"806","author":"CY Cui","year":"2022","unstructured":"Cui, C. Y. et al. Comprehensive analysis of plasmid-mediated tet(X4)-positive Escherichia coli isolates from clinical settings revealed a high correlation with animals and environments-derived strains. Sci Total Environ 806, 150687. https:\/\/doi.org\/10.1016\/j.scitotenv.2021.150687 (2022).","journal-title":"Sci Total Environ"},{"key":"97424_CR25","doi-asserted-by":"publisher","first-page":"110145","DOI":"10.1016\/j.ijfoodmicro.2023.110145","volume":"391\u2013393","author":"C Yue","year":"2023","unstructured":"Yue, C. et al. First detection of tet(X4)-positive Enterobacterales in retail vegetables and clonal spread of Escherichia coli ST195 producing Tet(X4) in animals, foods, and humans across China and Thailand. Int J Food Microbiol 391\u2013393, 110145. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2023.110145 (2023).","journal-title":"Int J Food Microbiol"},{"key":"97424_CR26","doi-asserted-by":"publisher","first-page":"969769","DOI":"10.3389\/fmicb.2022.969769","volume":"13","author":"S Zhang","year":"2022","unstructured":"Zhang, S. et al. Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4). Front Microbiol 13, 969769. https:\/\/doi.org\/10.3389\/fmicb.2022.969769 (2022).","journal-title":"Front Microbiol"},{"key":"97424_CR27","doi-asserted-by":"publisher","first-page":"154010","DOI":"10.1016\/j.scitotenv.2022.154010","volume":"826","author":"D Liu","year":"2022","unstructured":"Liu, D. et al. Structural diversity of the ISCR2-mediated rolling-cycle transferable unit carrying tet(X4). Sci Total Environ 826, 154010. https:\/\/doi.org\/10.1016\/j.scitotenv.2022.154010 (2022).","journal-title":"Sci Total Environ"},{"key":"97424_CR28","doi-asserted-by":"publisher","first-page":"1893","DOI":"10.1111\/jam.14885","volume":"130","author":"G Li","year":"2021","unstructured":"Li, G. et al. Linoleic acid and alpha-linolenic acid inhibit conjugative transfer of an IncX4 plasmid carrying mcr-1. J Appl Microbiol 130, 1893\u20131901. https:\/\/doi.org\/10.1111\/jam.14885 (2021).","journal-title":"J Appl Microbiol"},{"key":"97424_CR29","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.jmb.2006.04.060","volume":"360","author":"S Hare","year":"2006","unstructured":"Hare, S., Bayliss, R., Baron, C. & Waksman, G. A large domain swap in the VirB11 ATPase of Brucella suis leaves the hexameric assembly intact. J Mol Biol 360, 56\u201366. https:\/\/doi.org\/10.1016\/j.jmb.2006.04.060 (2006).","journal-title":"J Mol Biol"},{"key":"97424_CR30","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1111\/mmi.13359","volume":"100","author":"J Ripoll-Rozada","year":"2016","unstructured":"Ripoll-Rozada, J. et al. Type IV traffic ATPase TrwD as molecular target to inhibit bacterial conjugation. Mol Microbiol 100, 912\u2013921. https:\/\/doi.org\/10.1111\/mmi.13359 (2016).","journal-title":"Mol Microbiol"},{"key":"97424_CR31","doi-asserted-by":"publisher","first-page":"1279359","DOI":"10.3389\/fbinf.2023.1279359","volume":"3","author":"S Algarni","year":"2023","unstructured":"Algarni, S. et al. Development of an antimicrobial resistance plasmid transfer gene database for enteric bacteria. Front Bioinform 3, 1279359. https:\/\/doi.org\/10.3389\/fbinf.2023.1279359 (2023).","journal-title":"Front Bioinform"},{"key":"97424_CR32","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","volume":"18","author":"AP Magiorakos","year":"2012","unstructured":"Magiorakos, A. P. et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 18, 268\u2013281. https:\/\/doi.org\/10.1111\/j.1469-0691.2011.03570.x (2012).","journal-title":"Clin Microbiol Infect"},{"key":"97424_CR33","doi-asserted-by":"publisher","unstructured":"Rebelo, A. R. et al. Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes. Euro Surveill 23, https:\/\/doi.org\/10.2807\/1560-7917.ES.2018.23.6.17-00672 (2018).","DOI":"10.2807\/1560-7917.ES.2018.23.6.17-00672"},{"key":"97424_CR34","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","volume":"70","author":"L Poirel","year":"2011","unstructured":"Poirel, L., Walsh, T. R., Cuvillier, V. & Nordmann, P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 70, 119\u2013123. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2010.12.002 (2011).","journal-title":"Diagn Microbiol Infect Dis"},{"key":"97424_CR35","first-page":"1504","volume":"94","author":"N Udomsantisuk","year":"2011","unstructured":"Udomsantisuk, N., Nunthapisud, P., Tirawatanapong, T. & Dansuputra, M. Molecular characterization of extended spectrum beta-lactamase among clinical isolates Escherichia coli and Klebsiella pneumoniae. J Med Assoc Thai 94, 1504\u20131512 (2011).","journal-title":"J Med Assoc Thai"},{"key":"97424_CR36","doi-asserted-by":"publisher","first-page":"1936","DOI":"10.1128\/AAC.44.7.1936-1942.2000","volume":"44","author":"R Bonnet","year":"2000","unstructured":"Bonnet, R. et al. A novel CTX-M beta-lactamase (CTX-M-8) in cefotaxime-resistant enterobacteriaceae isolated in Brazil. Antimicrob Agents Chemother 44, 1936\u20131942 (2000).","journal-title":"Antimicrob Agents Chemother"},{"key":"97424_CR37","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/S0378-1097(03)00306-9","volume":"223","author":"K Colom","year":"2003","unstructured":"Colom, K. et al. Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA-1 genes in Enterobacteriaceae. FEMS Microbiol. Lett. 223, 147\u2013151 (2003).","journal-title":"FEMS Microbiol. Lett."},{"key":"97424_CR38","doi-asserted-by":"publisher","first-page":"2210","DOI":"10.1128\/AAC.34.11.2210","volume":"34","author":"C Mabilat","year":"1990","unstructured":"Mabilat, C. & Courvalin, P. Development of \u201coligotyping\u201d for characterization and molecular epidemiology of TEM beta-lactamases in members of the family Enterobacteriaceae. Antimicrob Agents Chemother 34, 2210\u20132216 (1990).","journal-title":"Antimicrob Agents Chemother"},{"key":"97424_CR39","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1099\/jmm.0.46160-0","volume":"54","author":"L Xu","year":"2005","unstructured":"Xu, L., Ensor, V., Gossain, S., Nye, K. & Hawkey, P. Rapid and simple detection of blaCTX-M genes by multiplex PCR assay. J Med Microbiol 54, 1183\u20131187. https:\/\/doi.org\/10.1099\/jmm.0.46160-0 (2005).","journal-title":"J Med Microbiol"},{"key":"97424_CR40","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/j.ijmm.2018.03.008","volume":"308","author":"S Luk-In","year":"2018","unstructured":"Luk-In, S. et al. High prevalence of ceftriaxone resistance among invasive Salmonella enterica serotype Choleraesuis isolates in Thailand: The emergence and increase of CTX-M-55 in ciprofloxacin-resistant S. Choleraesuis isolates. Int J Med Microbiol 308, 447\u2013453. https:\/\/doi.org\/10.1016\/j.ijmm.2018.03.008 (2018).","journal-title":"Int J Med Microbiol"},{"key":"97424_CR41","doi-asserted-by":"publisher","first-page":"2661","DOI":"10.1128\/AAC.42.10.2661","volume":"42","author":"LM Weigel","year":"1998","unstructured":"Weigel, L. M., Steward, C. D. & Tenover, F. C. gyrA mutations associated with fluoroquinolone resistance in eight species of Enterobacteriaceae. Antimicrob Agents Chemother 42, 2661\u20132667. https:\/\/doi.org\/10.1128\/AAC.42.10.2661 (1998).","journal-title":"Antimicrob Agents Chemother"},{"key":"97424_CR42","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1016\/j.ijantimicag.2006.11.008","volume":"29","author":"C Lascols","year":"2007","unstructured":"Lascols, C. et al. Type II topoisomerase mutations in clinical isolates of Enterobacter cloacae and other enterobacterial species harbouring the qnrA gene. Int J Antimicrob Agents 29, 402\u2013409. https:\/\/doi.org\/10.1016\/j.ijantimicag.2006.11.008 (2007).","journal-title":"Int J Antimicrob Agents"},{"key":"97424_CR43","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1093\/jac\/dkl213","volume":"58","author":"C Kehrenberg","year":"2006","unstructured":"Kehrenberg, C., Friederichs, S., de Jong, A., Michael, G. B. & Schwarz, S. Identification of the plasmid-borne quinolone resistance gene qnrS in Salmonella enterica serovar Infantis. J Antimicrob Chemother 58, 18\u201322. https:\/\/doi.org\/10.1093\/jac\/dkl213 (2006).","journal-title":"J Antimicrob Chemother"},{"key":"97424_CR44","doi-asserted-by":"publisher","first-page":"2233","DOI":"10.1128\/jcm.33.9.2233-2239.1995","volume":"33","author":"FC Tenover","year":"1995","unstructured":"Tenover, F. C. et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J Clin Microbiol 33, 2233\u20132239 (1995).","journal-title":"J Clin Microbiol"},{"key":"97424_CR45","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.mimet.2005.03.018","volume":"63","author":"A Carattoli","year":"2005","unstructured":"Carattoli, A. et al. Identification of plasmids by PCR-based replicon typing. J. Microbiol. Methods 63, 219\u2013228. https:\/\/doi.org\/10.1016\/j.mimet.2005.03.018 (2005).","journal-title":"J. Microbiol. Methods"},{"key":"97424_CR46","doi-asserted-by":"publisher","first-page":"4394","DOI":"10.1128\/AAC.00444-16","volume":"60","author":"L Poirel","year":"2016","unstructured":"Poirel, L. et al. Genetic features of MCR-1-producing colistin-resistant Escherichia coli isolates in South Africa. Antimicrob Agents Chemother 60, 4394\u20134397. https:\/\/doi.org\/10.1128\/AAC.00444-16 (2016).","journal-title":"Antimicrob Agents Chemother"},{"key":"97424_CR47","doi-asserted-by":"crossref","unstructured":"Martin, M. Cut adapt removes adapter sequences from high-throughput sequencing reads. EMBnet.journal 17, 10\u201312 (2011).","DOI":"10.14806\/ej.17.1.200"},{"key":"97424_CR48","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1089\/cmb.2012.0021","volume":"19","author":"A Bankevich","year":"2012","unstructured":"Bankevich, A. et al. SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19, 455\u2013477. https:\/\/doi.org\/10.1089\/cmb.2012.0021 (2012).","journal-title":"J Comput Biol"},{"key":"97424_CR49","doi-asserted-by":"publisher","first-page":"e000132","DOI":"10.1099\/mgen.0.000132","volume":"3","author":"RR Wick","year":"2017","unstructured":"Wick, R. R., Judd, L. M., Gorrie, C. L. & Holt, K. E. Completing bacterial genome assemblies with multiplex MinION sequencing. Microb Genom 3, e000132. https:\/\/doi.org\/10.1099\/mgen.0.000132 (2017).","journal-title":"Microb Genom"},{"key":"97424_CR50","doi-asserted-by":"publisher","unstructured":"De Coster, W. & Rademakers, R. NanoPack2: Population-scale evaluation of long-read sequencing data. Bioinformatics 39. https:\/\/doi.org\/10.1093\/bioinformatics\/btad311 (2023).","DOI":"10.1093\/bioinformatics\/btad311"},{"key":"97424_CR51","doi-asserted-by":"publisher","first-page":"e1005595","DOI":"10.1371\/journal.pcbi.1005595","volume":"13","author":"RR Wick","year":"2017","unstructured":"Wick, R. R., Judd, L. M., Gorrie, C. L. & Holt, K. E. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 13, e1005595. https:\/\/doi.org\/10.1371\/journal.pcbi.1005595 (2017).","journal-title":"PLoS Comput Biol"},{"key":"97424_CR52","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","volume":"30","author":"T Seemann","year":"2014","unstructured":"Seemann, T. Prokka: Rapid prokaryotic genome annotation. Bioinformatics 30, 2068\u20132069. https:\/\/doi.org\/10.1093\/bioinformatics\/btu153 (2014).","journal-title":"Bioinformatics"},{"key":"97424_CR53","doi-asserted-by":"publisher","unstructured":"Bharat, A. et al. Correlation between phenotypic and in silico detection of antimicrobial resistance in Salmonella enterica in Canada using staramr. Microorganisms 10. https:\/\/doi.org\/10.3390\/microorganisms10020292 (2022).","DOI":"10.3390\/microorganisms10020292"},{"key":"97424_CR54","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1186\/s13059-014-0524-x","volume":"15","author":"TJ Treangen","year":"2014","unstructured":"Treangen, T. J., Ondov, B. D., Koren, S. & Phillippy, A. M. The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes. Genome Biol 15, 524. https:\/\/doi.org\/10.1186\/s13059-014-0524-x (2014).","journal-title":"Genome Biol"},{"key":"97424_CR55","doi-asserted-by":"publisher","first-page":"e15","DOI":"10.1093\/nar\/gku1196","volume":"43","author":"NJ Croucher","year":"2015","unstructured":"Croucher, N. J. et al. Rapid phylogenetic analysis of large samples of recombinant bacterial whole genome sequences using Gubbins. Nucleic Acids Res 43, e15. https:\/\/doi.org\/10.1093\/nar\/gku1196 (2015).","journal-title":"Nucleic Acids Res"},{"key":"97424_CR56","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","volume":"30","author":"A Stamatakis","year":"2014","unstructured":"Stamatakis, A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312\u20131313. https:\/\/doi.org\/10.1093\/bioinformatics\/btu033 (2014).","journal-title":"Bioinformatics"},{"key":"97424_CR57","doi-asserted-by":"publisher","first-page":"W78","DOI":"10.1093\/nar\/gkae268","volume":"52","author":"I Letunic","year":"2024","unstructured":"Letunic, I. & Bork, P. Interactive Tree of Life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool. Nucleic Acids Res 52, W78\u2013W82. https:\/\/doi.org\/10.1093\/nar\/gkae268 (2024).","journal-title":"Nucleic Acids Res"},{"key":"97424_CR58","doi-asserted-by":"publisher","first-page":"4195","DOI":"10.1128\/JB.00437-13","volume":"195","author":"J Ripoll-Rozada","year":"2013","unstructured":"Ripoll-Rozada, J., Zunzunegui, S., de la Cruz, F., Arechaga, I. & Cabezon, E. Functional interactions of VirB11 traffic ATPases with VirB4 and VirD4 molecular motors in type IV secretion systems. J Bacteriol 195, 4195\u20134201. https:\/\/doi.org\/10.1128\/JB.00437-13 (2013).","journal-title":"J Bacteriol"},{"key":"97424_CR59","doi-asserted-by":"publisher","first-page":"2785","DOI":"10.1002\/jcc.21256","volume":"30","author":"GM Morris","year":"2009","unstructured":"Morris, G. M. et al. AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility. J Comput Chem 30, 2785\u20132791. https:\/\/doi.org\/10.1002\/jcc.21256 (2009).","journal-title":"J Comput Chem"},{"key":"97424_CR60","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen, E. F. et al. UCSF Chimera\u2013a visualization system for exploratory research and analysis. J Comput Chem 25, 1605\u20131612. https:\/\/doi.org\/10.1002\/jcc.20084 (2004).","journal-title":"J Comput Chem"},{"key":"97424_CR61","doi-asserted-by":"publisher","unstructured":"Hoffmann, A. & Dimroth, P. The ATPase of Bacillus alcalophilus. Purification and properties of the enzyme. Eur. J. Biochem. 194, 423\u2013430, https:\/\/doi.org\/10.1111\/j.1432-1033.1990.tb15635.x (1990).","DOI":"10.1111\/j.1432-1033.1990.tb15635.x"},{"key":"97424_CR62","doi-asserted-by":"publisher","first-page":"e3705","DOI":"10.21769\/BioProtoc.3705","volume":"10","author":"A Kaundal","year":"2020","unstructured":"Kaundal, A., Ramu, V. S. & Mysore, K. S. The ATPase activity of Escherichia coli expressed AAA(+)-ATPase protein. Bio Protoc 10, e3705. https:\/\/doi.org\/10.21769\/BioProtoc.3705 (2020).","journal-title":"Bio Protoc"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-97424-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-97424-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-97424-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T23:02:47Z","timestamp":1768345367000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-97424-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,15]]},"references-count":62,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["97424"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-97424-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,15]]},"assertion":[{"value":"24 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 January 2026","order":5,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Update","order":6,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The original online version of this Article was revised: The Funding section in the original version of this Article was incomplete. The Funding section now reads: \u201cThis research project is supported by Mahidol University (Fundamental Fund: fiscal year 2023 by National Science Research and Innovation Fund (NSRF)). This project is funded by the National Research Council of Thailand (NRCT): Grant No. N42A660963. This work was also supported by the grant for research and innovation from the National Research Council of Thailand (NRCT) and the Temporary Research Assistantship from the Faculty of Medical Technology, Mahidol University\". The original Article has been corrected.","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The study was conducted by the Biosafety guidelines and approved by the Mahidol University-Institutional Biosafety Committee (MU-IBC), Mahidol University (Nakhon Pathom, Thailand) [MU 2023-009]. This study of anonymised clinical isolates did not include interaction or intervention with human subjects or access to identifiable private information. The Mahidol University Central Institutional Review Board (MU-CIRB), Mahidol University (Nakhon Pathom, Thailand) waived the requirement for IRB review.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"For this retrospective study of anonymous clinical isolates, the requirement for informed consent from patients was waived by the Mahidol University Central Institutional Review Board (MU-CIRB), Mahidol University (Nakhon Pathom, Thailand).","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}],"article-number":"12892"}}