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In this study, we investigated the occurrence and genetic basis of antimicrobial resistance in a Magellanic Penguin (<jats:italic>Spheniscus magellanicus<\/jats:italic>) rescued off the coast of Rio de Janeiro, Brazil. The penguin presented a bacterial infection, identified as <jats:italic>Klebsiella pneumoniae<\/jats:italic>. Molecular analysis revealed the presence of several resistance genes, including those that confer resistance to carbapenems, beta-lactams, quinolones, and other classes of antibiotics. The bacterial strain belonged to Sequence Type 70 (ST70), a clone previously associated with human nosocomial infections. This study highlights the potential of migratory penguins as vectors of antimicrobial-resistant microorganisms, emphasizing the need for a One Health approach to address the complex interaction between environmental factors, animal health, and human well-being. The findings underscore the urgency of implementing strategies to mitigate the spread of multidrug-resistant bacteria in natural and urban environments.<\/jats:p>","DOI":"10.1038\/s41598-025-97816-4","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T15:05:40Z","timestamp":1751382340000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multidrug-resistant Klebsiella pneumoniae ST70 harboring blaNDM in a migratory Penguin"],"prefix":"10.1038","volume":"15","author":[{"given":"Sandryelle Merces","family":"Freire","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annelise","family":"Kyllar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"C\u00f4rtes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago P. 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