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Investigating quantitative changes in soluble biomarkers associated with <jats:italic>H. pylori<\/jats:italic> infection offers a promising method for monitoring the progression of the infection, inflammatory response and potentially systemic consequences. This study aimed to identify, using an experimental model of <jats:italic>H. pylori<\/jats:italic> infection in guinea pigs, the specific metabolomic biomarkers in the serum of <jats:italic>H. pylori<\/jats:italic>-infected (32) versus uninfected (32) animals. The <jats:italic>H. pylori<\/jats:italic> status was confirmed through histological, molecular, and serological examinations. Metabolomic profiling was conducted using UPLC-QTOF\/MS methods. The metabolomic biomarkers significantly associated with <jats:italic>H. pylori<\/jats:italic> infection were selected based on volcano plots and traditional univariate receiver operating characteristics (ROC). This study identified 12 unique metabolites significantly differentiating <jats:italic>H. pylori<\/jats:italic>-infected guinea pigs from uninfected ones. In summary, the metabolomic profiling of serum samples, in combination with ROC characteristics of the data, enhances the monitoring of <jats:italic>H. pylori<\/jats:italic> infection and related inflammatory responses in guinea pigs experimentally infected with these bacteria, with potential applications in humans for prediction the infection course and its systemic effects.<\/jats:p>","DOI":"10.1038\/s41598-025-98016-w","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T06:40:37Z","timestamp":1744699237000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Untargeted metabolomic profiling for identifying systemic signatures of helicobacter pylori infection in a guinea pig model"],"prefix":"10.1038","volume":"15","author":[{"given":"Weronika","family":"Gonciarz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucyna","family":"Kozlowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"R\u00f3g","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Chmiela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"98016_CR1","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1038\/nrd.2016.32","volume":"5","author":"DS Wishart","year":"2016","unstructured":"Wishart, D. 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