{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T08:44:42Z","timestamp":1774773882286,"version":"3.50.1"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-98151-4","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T09:17:02Z","timestamp":1745227022000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of a novel multiepitope vaccine against Menangle virus (MenV) using in-silico approaches by targeting its transmembrane proteins"],"prefix":"10.1038","volume":"15","author":[{"given":"Muhammad","family":"Naveed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adeeba","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tariq","family":"Aziz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noor","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Amjad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Furrmein","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayaz Ali","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Metab","family":"Alharbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thamer H.","family":"Albekairi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah F.","family":"Alasmari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,21]]},"reference":[{"issue":"12","key":"98151_CR1","doi-asserted-by":"publisher","first-page":"2590","DOI":"10.1099\/vir.0.045385-0","volume":"93","author":"JA Barr","year":"2012","unstructured":"Barr, J. A., Smith, C., Marsh, G. A., Field, H. & Wang, L. F. Evidence of Bat origin for menangle virus, a zoonotic paramyxovirus first isolated from diseased pigs. J. Gen. Virol. 93 (12), 2590\u20132594. https:\/\/doi.org\/10.1099\/vir.0.045385-0 (2012).","journal-title":"J. Gen. Virol."},{"issue":"9","key":"98151_CR2","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.3390\/v13091737","volume":"13","author":"MN Webby","year":"2021","unstructured":"Webby, M. N. et al. Structural analysis of the menangle virus P protein reveals a soft boundary between ordered and disordered regions. Viruses 13 (9), 1737. https:\/\/doi.org\/10.3390\/v13091737 (2021).","journal-title":"Viruses"},{"issue":"8","key":"98151_CR3","doi-asserted-by":"publisher","first-page":"2857","DOI":"10.1080\/07391102.2020.1756411","volume":"39","author":"M Enayatkhani","year":"2021","unstructured":"Enayatkhani, M. et al. Reverse vaccinology approach to design a novel multi-epitope vaccine candidate against COVID-19: an in Silico study. J. Biomol. Struct. Dynamics. 39 (8), 2857\u20132872. https:\/\/doi.org\/10.1080\/07391102.2020.1756411 (2021).","journal-title":"J. Biomol. Struct. Dynamics"},{"issue":"8","key":"98151_CR4","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1080\/14760584.2020.1794832","volume":"19","author":"K Kardani","year":"2020","unstructured":"Kardani, K., Bolhassani, A. & Namvar, A. An overview of in Silico vaccine design against different pathogens and cancer. Expert Rev. Vaccines. 19 (8), 699\u2013726. https:\/\/doi.org\/10.1080\/14760584.2020.1794832 (2020).","journal-title":"Expert Rev. Vaccines"},{"issue":"1","key":"98151_CR5","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1071\/MA17007","volume":"38","author":"PD Kirkland","year":"2017","unstructured":"Kirkland, P. D. Menangle virus: one of the first of the novel viruses from fruit bats. Microbiol. Australia. 38 (1), 22. https:\/\/doi.org\/10.1071\/MA17007 (2017).","journal-title":"Microbiol. Australia"},{"issue":"5","key":"98151_CR6","doi-asserted-by":"publisher","first-page":"537","DOI":"10.2217\/fmb.09.26","volume":"4","author":"ER Virtue","year":"2009","unstructured":"Virtue, E. R., Marsh, G. A. & Wang, L. F. Paramyxoviruses infecting humans: the old, the new and the unknown. Future Microbiol. 4 (5), 537\u2013554. https:\/\/doi.org\/10.2217\/fmb.09.26 (2009).","journal-title":"Future Microbiol."},{"key":"98151_CR7","doi-asserted-by":"publisher","unstructured":"Martinelli, D. D. In silico vaccine design: A tutorial in immunoinformatics. Healthc. Anal. 2, 100044 (2022). https:\/\/doi.org\/10.1016\/j.health.2022.100044","DOI":"10.1016\/j.health.2022.100044"},{"issue":"1","key":"98151_CR8","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1007\/s11686-023-00771-1","volume":"69","author":"M Naveed","year":"2024","unstructured":"Naveed, M. et al. An Aedes\u2013Anopheles vaccine candidate supplemented with BCG epitopes against the Aedes and Anopheles genera to overcome hypersensitivity to mosquito bites. Acta Parasitol. 69 (1), 483\u2013504. https:\/\/doi.org\/10.1007\/s11686-023-00771-1 (2024).","journal-title":"Acta Parasitol."},{"issue":"8","key":"98151_CR9","doi-asserted-by":"publisher","first-page":"1300","DOI":"10.3390\/vaccines10081300","volume":"10","author":"M Naveed","year":"2022","unstructured":"Naveed, M. et al. Designing a novel Peptide-Based Multi-Epitope vaccine to evoke a robust immune response against pathogenic Multidrug-Resistant Providencia Heimbachae. Vaccines 10 (8), 1300. https:\/\/doi.org\/10.3390\/vaccines10081300 (2022a).","journal-title":"Vaccines"},{"issue":"10","key":"98151_CR10","doi-asserted-by":"publisher","first-page":"1732","DOI":"10.3390\/vaccines10101732","volume":"10","author":"M Naveed","year":"2022","unstructured":"Naveed, M. et al. Immunoinformatics approach to design Multi-Epitope-Based vaccine against Machupo virus taking viral nucleocapsid as a potential candidate. Vaccines 10 (10), 1732. https:\/\/doi.org\/10.3390\/vaccines10101732 (2022).","journal-title":"Vaccines"},{"key":"98151_CR11","doi-asserted-by":"publisher","first-page":"117793222311825","DOI":"10.1177\/11779322231182560","volume":"17","author":"H Muhammad Rehman","year":"2023","unstructured":"Muhammad Rehman, H. et al. In Silico investigation of a chimeric IL24-LK6 fusion protein as a potent candidate against breast cancer. Bioinform. Biol. Insights. 17, 11779322231182560. https:\/\/doi.org\/10.1177\/11779322231182560 (2023).","journal-title":"Bioinform. Biol. Insights"},{"issue":"8","key":"98151_CR12","doi-asserted-by":"publisher","first-page":"1300","DOI":"10.3390\/vaccines10081300","volume":"10","author":"M Naveed","year":"2022","unstructured":"Naveed, M. et al. Designing a novel Peptide-Based Multi-Epitope vaccine to evoke a robust immune response against pathogenic Multidrug-Resistant Providencia Heimbachae. Vaccines 10 (8), 1300. https:\/\/doi.org\/10.3390\/vaccines10081300 (2022b).","journal-title":"Vaccines"},{"key":"98151_CR13","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2020_6721","author":"M Naveed","year":"2023","unstructured":"Naveed, M. et al. An mRNA-based reverse-vaccinology strategy to stimulate the immune response against Nipah virus in humans using fusion glycoproteins. Acta Biochim. Pol. https:\/\/doi.org\/10.18388\/abp.2020_6721 (2023).","journal-title":"Acta Biochim. Pol."},{"issue":"5","key":"98151_CR14","doi-asserted-by":"publisher","first-page":"176","DOI":"10.31083\/j.fbl2905176","volume":"29","author":"T Aziz","year":"2024","unstructured":"Aziz, T. et al. Designing a multiepitope vaccine against the foodborne pathogenic bacteria Listeria monocytogenes using subtractive immunoinformatics approaches. Front. Bioscience-Landmark. 29 (5), 176. https:\/\/doi.org\/10.31083\/j.fbl2905176 (2024).","journal-title":"Front. Bioscience-Landmark"},{"issue":"5","key":"98151_CR15","doi-asserted-by":"publisher","first-page":"664","DOI":"10.3390\/vaccines10050664","volume":"10","author":"M Naveed","year":"2022","unstructured":"Naveed, M. et al. A vaccine construction against COVID-19-Associated mucormycosis contrived with Immunoinformatics-Based scavenging of potential Mucoralean epitopes. Vaccines 10 (5), 664. https:\/\/doi.org\/10.3390\/vaccines10050664 (2022).","journal-title":"Vaccines"},{"issue":"1","key":"98151_CR16","doi-asserted-by":"publisher","first-page":"28445","DOI":"10.1038\/s41598-024-79275-5","volume":"14","author":"H Sadia","year":"2024","unstructured":"Sadia, H. et al. Natural AI-based drug designing by modification of ascorbic acid and Curcumin to combat buprofezin toxicity by using molecular dynamics study. Sci. Rep. 14 (1), 28445. https:\/\/doi.org\/10.1038\/s41598-024-79275-5 (2024).","journal-title":"Sci. Rep."},{"issue":"3","key":"98151_CR17","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1111\/j.1751-0813.2001.tb14580.x","volume":"79","author":"P Kirkland","year":"2001","unstructured":"Kirkland, P. et al. Epidemiology and control of menangle virus in pigs. Aust. Vet. J. 79 (3), 199\u2013206. https:\/\/doi.org\/10.1111\/j.1751-0813.2001.tb14580.x (2001).","journal-title":"Aust. Vet. J."},{"issue":"1\u20132","key":"98151_CR18","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/S0378-1135(99)00063-2","volume":"68","author":"K Halpin","year":"1999","unstructured":"Halpin, K., Young, P. L., Field, H. & Mackenzie, J. S. Newly discovered viruses of flying foxes. Vet. Microbiol. 68 (1\u20132), 83\u201387. https:\/\/doi.org\/10.1016\/S0378-1135(99)00063-2 (1999).","journal-title":"Vet. Microbiol."},{"issue":"5","key":"98151_CR19","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1099\/vir.0.038448-0","volume":"93","author":"TR Bowden","year":"2012","unstructured":"Bowden, T. R., Bingham, J., Harper, J. A. & Boyle, D. B. Menangle virus, a pteropid Bat paramyxovirus infectious for pigs and humans, exhibits tropism for secondary lymphoid organs and intestinal epithelium in weaned pigs. J. Gen. Virol. 93 (5), 1007\u20131016. https:\/\/doi.org\/10.1099\/vir.0.038448-0 (2012).","journal-title":"J. Gen. Virol."},{"issue":"4","key":"98151_CR20","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1111\/j.1751-0813.2007.00131.x","volume":"85","author":"A Philbey","year":"2007","unstructured":"Philbey, A., Ross, A., Kirkland, P. & Love, R. Skeletal and neurological malformations in pigs congenitally infected with menangle virus. Aust. Vet. J. 85 (4), 134\u2013140. https:\/\/doi.org\/10.1111\/j.1751-0813.2007.00131.x (2007).","journal-title":"Aust. Vet. J."},{"key":"98151_CR21","doi-asserted-by":"publisher","first-page":"2698","DOI":"10.3389\/fimmu.2019.02698","volume":"10","author":"L Michel-Tod\u00f3","year":"2019","unstructured":"Michel-Tod\u00f3, L. et al. In Silico design of an Epitope-Based vaccine ensemble for Chagas disease. Front. Immunol. 10, 2698. https:\/\/doi.org\/10.3389\/fimmu.2019.02698 (2019).","journal-title":"Front. Immunol."},{"issue":"5","key":"98151_CR22","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1007\/s00705-008-0059-0","volume":"153","author":"KC Yaiw","year":"2008","unstructured":"Yaiw, K. C. et al. Viral morphogenesis and morphological changes in human neuronal cells following Tioman and menangle virus infection. Arch. Virol. 153 (5), 865\u2013875. https:\/\/doi.org\/10.1007\/s00705-008-0059-0 (2008).","journal-title":"Arch. Virol."},{"issue":"1","key":"98151_CR23","doi-asserted-by":"publisher","first-page":"7757","DOI":"10.1038\/s41598-022-11851-z","volume":"12","author":"M Alizadeh","year":"2022","unstructured":"Alizadeh, M. et al. Designing a novel multi\u2013epitope vaccine against Ebola virus using reverse vaccinology approach. Sci. Rep. 12 (1), 7757. https:\/\/doi.org\/10.1038\/s41598-022-11851-z (2022).","journal-title":"Sci. Rep."},{"issue":"5","key":"98151_CR24","doi-asserted-by":"publisher","first-page":"e0300507","DOI":"10.1371\/journal.pone.0300507","volume":"19","author":"A Kumar","year":"2024","unstructured":"Kumar, A., Misra, G., Mohandas, S. & Yadav, P. D. Multi-epitope vaccine design using in Silico analysis of glycoprotein and nucleocapsid of NIPAH virus. PLOS ONE. 19 (5), e0300507. https:\/\/doi.org\/10.1371\/journal.pone.0300507 (2024).","journal-title":"PLOS ONE"},{"issue":"1","key":"98151_CR25","doi-asserted-by":"publisher","first-page":"e19371","DOI":"10.2196\/19371","volume":"1","author":"S Srivastava","year":"2020","unstructured":"Srivastava, S. et al. Structural basis for designing multiepitope vaccines against COVID-19 infection: in Silico vaccine design and validation. JMIR Bioinf. Biotechnol. 1 (1), e19371. https:\/\/doi.org\/10.2196\/19371 (2020).","journal-title":"JMIR Bioinf. Biotechnol."},{"issue":"6","key":"98151_CR26","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/j.apjtm.2017.06.016","volume":"10","author":"CK Saha","year":"2017","unstructured":"Saha, C. K., Hasan, M., Hossain, M. S., Jahan, M. A., Azad, A. K. & Md., & In Silico identification and characterization of common epitope-based peptide vaccine for Nipah and Hendra viruses. Asian Pac. J. Trop. Med. 10 (6), 529\u2013538. https:\/\/doi.org\/10.1016\/j.apjtm.2017.06.016 (2017).","journal-title":"Asian Pac. J. Trop. Med."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98151-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98151-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98151-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T09:17:03Z","timestamp":1745227023000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98151-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,21]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["98151"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-98151-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,21]]},"assertion":[{"value":"10 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13715"}}