{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T10:50:38Z","timestamp":1782816638953,"version":"3.54.5"},"reference-count":101,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004360","name":"Swedish University of Agricultural Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004360","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Elevation in CO<jats:sub>2<\/jats:sub> can significantly impact the biology of various organisms, affecting life-history traits of both aquatic and terrestrial forms, including disease-vectoring mosquitoes. For mosquitoes, this effect is accentuated by egg quiescence duration, resulting in a change in foraging of adult females. Female mosquitoes rely on their olfactory system for locating resources, such as nectar and blood. This study employs a transcriptomic approach to investigate how a projected elevation in CO<jats:sub>2<\/jats:sub> level, under a worst-case scenario, interacts with extended egg quiescence duration to modulate the molecular machinery of the peripheral olfactory system, the antennae and maxillary palps, of the yellow fever mosquito, <jats:italic>Aedes aegypti<\/jats:italic>. The transcriptome analysis demonstrates significant changes in the abundance of genes related to metabolism, xenobiotics degradation and chemosensory function, with the most pronounced effects observed in the CO<jats:sub>2<\/jats:sub> sensing tissue, the maxillary palp. The study provides novel insights into how anthropogenic climate change can modulate the olfactory sensory system of disease vectors, which may have cascading effects on resource-seeking behaviour.<\/jats:p>","DOI":"10.1038\/s41598-025-98159-w","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T12:33:15Z","timestamp":1745497995000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of elevated CO2 level and egg quiescence duration on gene expression in the peripheral olfactory system of Aedes aegypti"],"prefix":"10.1038","volume":"15","author":[{"given":"Sukritha","family":"Nalikkaramal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon Rose","family":"Hill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rickard","family":"Ignell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"98159_CR1","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1007\/s40725-018-0075-6","volume":"4","author":"DS Pureswaran","year":"2018","unstructured":"Pureswaran, D. S., Roques, A. & Battisti, A. Forest insects and climate change. Curr. Forestry Rep. 4, 35\u201350 (2018).","journal-title":"Curr. Forestry Rep."},{"key":"98159_CR2","doi-asserted-by":"crossref","unstructured":"Halsch, C. A. et al. Insects and recent climate change. Proc. Natl. Acad. Sci. U S A. 118, 2002543117, (2021).","DOI":"10.1073\/pnas.2002543117"},{"key":"98159_CR3","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.pt.2017.11.006","volume":"34","author":"NB Tjaden","year":"2018","unstructured":"Tjaden, N. B., Caminade, C., Beierkuhnlein, C. & Thomas, S. M. Mosquito-borne diseases: advances in modelling climate-change impacts. Trends Parasitol. 34, 227\u2013245 (2018).","journal-title":"Trends Parasitol."},{"key":"98159_CR4","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.cub.2017.12.022","volume":"28","author":"LC Weiss","year":"2018","unstructured":"Weiss, L. C. et al. Rising pCO2 in freshwater ecosystems has the potential to negatively affect predator-induced defenses in Daphnia. Curr. Biol. 28, 327\u2013332 (2018).","journal-title":"Curr. Biol."},{"key":"98159_CR5","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.aspen.2010.08.001","volume":"14","author":"YF Sun","year":"2011","unstructured":"Sun, Y. F. & Ge, F. How do aphids respond to elevated CO2?. J. Asia-Pac. Entomol. 14, 217\u2013220 (2011).","journal-title":"J. Asia-Pac. Entomol."},{"key":"98159_CR6","doi-asserted-by":"publisher","first-page":"9310","DOI":"10.1038\/s41598-025-92193-4","volume":"15","author":"S Nalikkaramal","year":"2025","unstructured":"Nalikkaramal, S., Hill, S. R. & Ignell, R. Effect of extended egg quiescence and elevation in carbon dioxide on life history traits of Aedes aegypti. Sci Rep 15, 9310. https:\/\/doi.org\/10.1038\/s41598-025-92193-4 (2025).","journal-title":"Sci Rep"},{"key":"98159_CR7","doi-asserted-by":"publisher","unstructured":"Allen, M. R. et al. Framing and context. In\u00a0Global warming of 1.5\u00b0c. an IPCC special report on the impacts of global warming of 1.5\u00b0c above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate poverty\u00a0(ed. Masson-Delmotte, V. et al.). 49\u201392 (Cambridge University Press).\u00a0https:\/\/doi.org\/10.1017\/9781009157940.003. (2018).","DOI":"10.1017\/9781009157940.003"},{"key":"98159_CR8","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1186\/1471-213X-8-82","volume":"8","author":"GL Rezende","year":"2008","unstructured":"Rezende, G. L. et al. Embryonic desiccation resistance in Aedes aegypti: presumptive role of the chitinized serosal cuticle. BMC Dev. Biol. 8, 82 (2008).","journal-title":"BMC Dev. Biol."},{"key":"98159_CR9","unstructured":"Clements, A. N. The biology of mosquitoes, volume 1(Chapman & Hall, 1992)."},{"key":"98159_CR10","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1590\/S0074-02762009000100020","volume":"104","author":"LC Farnesi","year":"2009","unstructured":"Farnesi, L. C., Martins, A. J., Valle, D. & Rezende, G. L. Embryonic development of Aedes aegypti (Diptera: Culicidae): influence of different constant temperatures. Mem. Inst. Oswaldo Cruz. 104, 124\u2013126 (2009).","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"98159_CR11","volume-title":"Aedes aegypti (L.) the yellow fever mosquito: its life history, bionomics and structure","author":"SR Christophers","year":"1960","unstructured":"Christophers, S. R. Aedes aegypti (L.) the yellow fever mosquito: its life history, bionomics and structure (Cambridge Univ. Press, 1960)."},{"key":"98159_CR12","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1016\/j.jinsphys.2012.03.005","volume":"58","author":"MH Perez","year":"2012","unstructured":"Perez, M. H. & Noriega, F. G. Aedes aegypti pharate 1st instar quiescence affects larval fitness and metal tolerance. J. Insect Physiol. 58, 824\u2013829 (2012).","journal-title":"J. Insect Physiol."},{"key":"98159_CR13","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.jinsphys.2012.12.007","volume":"59","author":"MH Perez","year":"2013","unstructured":"Perez, M. H. & Noriega, F. G. Aedes aegypti pharate 1st instar quiescence: a case for anticipatory reproductive plasticity. J. Insect Physiol. 59, 318\u2013324 (2013).","journal-title":"J. Insect Physiol."},{"key":"98159_CR14","doi-asserted-by":"publisher","first-page":"1330600","DOI":"10.3389\/fcimb.2023.1330600","volume":"13","author":"J Lewis","year":"2023","unstructured":"Lewis, J. et al. Intrinsic factors driving mosquito vector competence and viral evolution: a review. Front. Cell. Infect. Microbiol. 13, 1330600 (2023).","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"98159_CR15","doi-asserted-by":"publisher","first-page":"S86","DOI":"10.1016\/j.actatropica.2013.09.019","volume":"132","author":"DD Chadee","year":"1992","unstructured":"Chadee, D. D., Sutherland, J. M. & Gilles, J. R. L. Diel sugar-feeding and host-seeking rhythms in mosquitoes (Diptera: Culicidae) under laboratory conditions. Acta. Trop. 132, S86\u2013S90 (1992).","journal-title":"Acta. Trop."},{"key":"98159_CR16","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1007\/s00441-020-03368-6","volume":"383","author":"SR Hill","year":"2021","unstructured":"Hill, S. R. & Ignell, R. Modulation of odour-guided behaviour in mosquitoes. Cell Tissue Res. 383, 195\u2013206 (2021).","journal-title":"Cell Tissue Res."},{"key":"98159_CR17","first-page":"149","volume":"96","author":"JB Whittaker","year":"1999","unstructured":"Whittaker, J. B. Impacts and responses at population level of herbivorous insects to elevated CO2. Eur. J. Entomol. 96, 149\u2013156 (1999).","journal-title":"Eur. J. Entomol."},{"key":"98159_CR18","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1146\/annurev.en.34.010189.000525","volume":"34","author":"G Nicolas","year":"1989","unstructured":"Nicolas, G. & Sillans, D. Immediate and latent effects of carbon dioxide on insects. Ann. Rev. Entomol. 34, 97\u2013116 (1989).","journal-title":"Ann. Rev. Entomol."},{"key":"98159_CR19","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1111\/j.1365-2486.2005.00989.x","volume":"11","author":"L Abrell","year":"2005","unstructured":"Abrell, L., Guerenstein, P. G., Mechaber, W. L., Stange, G. & Christensen, T. A. Effect of elevated atmospheric CO2 on oviposition behaviour in Manduca sexta moths. Global. Change Biol. 11, 1272\u20131282 (2005).","journal-title":"Global. Change Biol."},{"key":"98159_CR20","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1007\/s004420050192","volume":"110","author":"G Stange","year":"1997","unstructured":"Stange, G. Effects of changes in atmospheric carbon dioxide on the location of hosts by the moth Cactoblastis cactorum. Oecologia 110, 539\u2013545 (1997).","journal-title":"Oecologia"},{"key":"98159_CR21","first-page":"598","volume":"217","author":"S Majeed","year":"2014","unstructured":"Majeed, S., Hill, S. R. & Ignell, R. Impact of elevated CO2 background levels on the host-seeking behaviour of Aedes aegypti. J. Exp. Biol. 217, 598\u2013604 (2014).","journal-title":"J. Exp. Biol."},{"key":"98159_CR22","doi-asserted-by":"publisher","first-page":"3908","DOI":"10.1038\/s41598-019-39748-4","volume":"9","author":"DAH Peach","year":"2019","unstructured":"Peach, D. A. H., Gries, R., Zhai, H., Young, N. & Gries, G. Multimodal floral cues guide mosquitoes to tansy inflorescences. Sci. Rep. 9, 3908 (2019).","journal-title":"Sci. Rep."},{"key":"98159_CR23","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1146\/annurev.ento.53.103106.093402","volume":"53","author":"PG Guerenstein","year":"2008","unstructured":"Guerenstein, P. G. & Hildebrand, J. G. Roles and effects of environmental carbon dioxide in insect life. Annu. Rev. Entomol. 53, 161\u2013178 (2008).","journal-title":"Annu. Rev. Entomol."},{"key":"98159_CR24","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1017\/S0007485300007811","volume":"70","author":"MT Gillies","year":"1980","unstructured":"Gillies, M. T. The role of carbon dioxide in host-finding by mosquitoes (Diptera: Culicidae): a review. Bull. Entomol. Res. 70, 525\u2013532 (1980).","journal-title":"Bull. Entomol. Res."},{"key":"98159_CR25","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1093\/chemse\/bjh035","volume":"29","author":"RB Barrozo","year":"2004","unstructured":"Barrozo, R. B. & Lazzari, C. R. The response of the blood-sucking bug Triatoma infestans to carbon dioxide and other host odours. Chem. Senses. 29, 319\u2013329 (2004).","journal-title":"Chem. Senses."},{"key":"98159_CR26","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1007\/BF00329801","volume":"102","author":"G Stange","year":"1995","unstructured":"Stange, G., Monro, J., Stowe, S. & Osmond, C. B. The CO2 sense of the moth Cactoblastis cactorum and its probable role in the biological control of the CAM plant Opuntia stricta. Oecologia 102, 341\u2013352 (1995).","journal-title":"Oecologia"},{"key":"98159_CR27","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1038\/nature02980","volume":"431","author":"GSB Suh","year":"2004","unstructured":"Suh, G. S. B. et al. A single population of olfactory sensory neurons mediates an innate avoidance behaviour in Drosophila. Nature 431, 854\u2013859 (2004).","journal-title":"Nature"},{"key":"98159_CR28","doi-asserted-by":"publisher","first-page":"2739","DOI":"10.1242\/jeb.02297","volume":"209","author":"C Faucher","year":"2006","unstructured":"Faucher, C., Forstreuter, M., Hilker, M. & de Bruyne, M. Behavioral responses of Drosophila to biogenic levels of carbon dioxide depend on life-stage, sex and olfactory context. J. Exp. Biol. 209, 2739\u20132748 (2006).","journal-title":"J. Exp. Biol."},{"key":"98159_CR29","doi-asserted-by":"publisher","first-page":"1820","DOI":"10.1111\/j.1365-2486.2004.00838.x","volume":"10","author":"EB Mondor","year":"2004","unstructured":"Mondor, E. B., Tremblay, M., Awmack, C. & Lindroth, R. L. Divergent pheromone-mediated insect behaviour under global atmospheric change. Glob. Change Biol. 10, 1820\u20131824 (2004).","journal-title":"Glob. Change Biol."},{"key":"98159_CR30","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1007\/s10886-017-0818-z","volume":"43","author":"A Boullis","year":"2017","unstructured":"Boullis, A. et al. Elevated carbon dioxide concentration reduces alarm signaling in aphids. J. Chem. Ecol. 43, 164\u2013171 (2017).","journal-title":"J. Chem. Ecol."},{"key":"98159_CR31","doi-asserted-by":"publisher","first-page":"345","DOI":"10.3389\/fmars.2020.00345","volume":"7","author":"JT Thomas","year":"2020","unstructured":"Thomas, J. T., Munday, P. L. & Watson, S. A. Toward a mechanistic understanding of marine invertebrate behaviour at elevated CO2. Front. Mar. Sci. 7, 345 (2020).","journal-title":"Front. Mar. Sci."},{"key":"98159_CR32","doi-asserted-by":"crossref","unstructured":"Heuer, R. M., Hamilton, T. J. & Nilsson, G. E. The physiology of behavioural impacts of high CO2. In\u00a0Fish Physiology. (eds. Grosell, M., Munday, P. L., Farrell, A. P. & Brauner, C. J.)\u00a037, 161\u2013194 (2019).","DOI":"10.1016\/bs.fp.2019.08.002"},{"key":"98159_CR33","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1038\/365699a0","volume":"365","author":"G Stange","year":"1993","unstructured":"Stange, G. & Wong, C. Moth response to climate. Nature 365, 699 (1993).","journal-title":"Nature"},{"key":"98159_CR34","doi-asserted-by":"publisher","first-page":"18710","DOI":"10.1073\/pnas.0905925106","volume":"106","author":"IT Heleniusa","year":"2009","unstructured":"Heleniusa, I. T. et al. Elevated CO2 suppresses specific Drosophila innate immune responses and resistance to bacterial infection. Proc. Natl. Acad. Sci. U.S.A. 106, 18710\u201318715 (2009).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"98159_CR35","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1093\/jmedent\/19.5.489","volume":"19","author":"S McIver","year":"1982","unstructured":"McIver, S. Sensilla of mosquitoes (Diptera: Culicidae). J. Med. Entomol. 19, 489\u2013535 (1982).","journal-title":"J. Med. Entomol."},{"key":"98159_CR36","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1146\/annurev-ento-120811-153635","volume":"58","author":"WS Leal","year":"2013","unstructured":"Leal, W. S. Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes. Annu. Rev. Entomol. 58, 373\u2013391 (2013).","journal-title":"Annu. Rev. Entomol."},{"key":"98159_CR37","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/s00441-020-03407-2","volume":"383","author":"M Wheelwright","year":"2021","unstructured":"Wheelwright, M., Whittle, C. R. & Riabinina, O. Olfactory systems across mosquito species. Cell Tissue Res. 383, 75\u201390 (2021).","journal-title":"Cell Tissue Res."},{"key":"98159_CR38","doi-asserted-by":"publisher","first-page":"3104","DOI":"10.1016\/j.cell.2022.07.024","volume":"185","author":"M Herre","year":"2022","unstructured":"Herre, M. et al. Non-canonical odour coding in the mosquito. Cell 185, 3104\u20133123 (2022).","journal-title":"Cell"},{"key":"98159_CR39","doi-asserted-by":"publisher","DOI":"10.1101\/2024.08.21.608847","author":"ED Adavi","year":"2024","unstructured":"Adavi, E. D. et al. Olfactory receptor coexpression and co-option in the dengue mosquito. Biorxiv https:\/\/doi.org\/10.1101\/2024.08.21.608847 (2024).","journal-title":"Biorxiv"},{"key":"98159_CR40","doi-asserted-by":"publisher","first-page":"e20","DOI":"10.1371\/journal.pbio.0040020","volume":"4","author":"R Benton","year":"2006","unstructured":"Benton, R., Sachse, S., Michnick, S. W. & Vosshall, L. B. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS Biol. 4, e20 (2006).","journal-title":"PLoS Biol."},{"key":"98159_CR41","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1016\/j.neuron.2004.08.019","volume":"43","author":"MC Larsson","year":"2004","unstructured":"Larsson, M. C. et al. Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction. Neuron 43, 703\u2013714 (2004).","journal-title":"Neuron"},{"key":"98159_CR42","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1038\/nature12206","volume":"498","author":"M DeGennaro","year":"2013","unstructured":"DeGennaro, M. et al. orco mutant mosquitoes lose strong preference for humans and are not repelled by volatile DEET. Nature 498, 487\u2013491 (2013).","journal-title":"Nature"},{"key":"98159_CR43","doi-asserted-by":"publisher","first-page":"4099","DOI":"10.1016\/j.cell.2022.09.034","volume":"185","author":"ME De Obaldia","year":"2022","unstructured":"De Obaldia, M. E. et al. Differential mosquito attraction to humans is associated with skin-derived carboxylic acid levels. Cell 185, 4099\u20134116 (2022).","journal-title":"Cell"},{"key":"98159_CR44","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1038\/nature13964","volume":"515","author":"CS McBride","year":"2014","unstructured":"McBride, C. S. et al. Evolution of mosquito preference for humans linked to an odorant receptor. Nature 515, 222\u2013227 (2014).","journal-title":"Nature"},{"key":"98159_CR45","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1016\/j.cub.2019.02.045","volume":"29","author":"JI Raji","year":"2019","unstructured":"Raji, J. I. et al. Aedes aegypti mosquitoes detect acidic volatiles found in human odor using the IR8a pathway. Curr Biol. 29, 1253\u20131262 (2019).","journal-title":"Curr Biol."},{"key":"98159_CR46","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1038\/nature05466","volume":"445","author":"WD Jones","year":"2007","unstructured":"Jones, W. D., Cayirlioglu, P., Kadow, I. G. & Vosshall, L. B. Two chemosensory receptors together mediate carbon dioxide detection in Drosophila. Nature 445, 86\u201390 (2007).","journal-title":"Nature"},{"key":"98159_CR47","doi-asserted-by":"publisher","first-page":"107510","DOI":"10.1016\/j.celrep.2020.03.074","volume":"31","author":"A Kumar","year":"2020","unstructured":"Kumar, A. et al. Contributions of the conserved insect carbon dioxide receptor subunits to odour detection. Cell Rep. 31, 107510 (2020).","journal-title":"Cell Rep."},{"key":"98159_CR48","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.1016\/j.cell.2013.12.044","volume":"156","author":"CJ McMeniman","year":"2014","unstructured":"McMeniman, C. J., Corfas, R. A., Matthews, B. J., Ritchie, S. A. & Vosshall, L. B. Multimodal integration of carbon dioxide and other sensory cues drives mosquito attraction to humans. Cell 156, 1060\u20131071 (2014).","journal-title":"Cell"},{"key":"98159_CR49","doi-asserted-by":"publisher","first-page":"2963","DOI":"10.1242\/jeb.01736","volume":"208","author":"T Dekker","year":"2005","unstructured":"Dekker, T., Geier, M. & Carde, R. T. Carbon dioxide instantly sensitises female yellow fever mosquitoes to human skin odours. J. Exp. Biol. 208, 2963\u20132972 (2005).","journal-title":"J. Exp. Biol."},{"key":"98159_CR50","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1126\/science.aay9847","volume":"365","author":"C Greppi","year":"2020","unstructured":"Greppi, C. et al. Mosquito heat seeking is driven by an ancestral cooling receptor. Science 365, 681\u2013684 (2020).","journal-title":"Science"},{"key":"98159_CR51","doi-asserted-by":"crossref","unstructured":"Matthews, B. J., Younger, M. A. & Vosshall, L. B. The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti. eLife. 8, e43963 (2019).","DOI":"10.7554\/eLife.43963"},{"key":"98159_CR52","doi-asserted-by":"crossref","unstructured":"Corfas, R. A. & Vosshall, L. B.\u00a0The cation channel TRPA1 tunes mosquito thermotaxis to host temperatures.\u00a0eLife.\u00a04, e11750 (2015).","DOI":"10.7554\/eLife.11750"},{"key":"98159_CR53","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1186\/s12864-020-07336-w","volume":"22","author":"SR Hill","year":"2021","unstructured":"Hill, S. R., Taparia, T. & Ignell, R. Regulation of the antennal transcriptome of the dengue vector, Aedes aegypti, during the first gonotrophic cycle. BMC Genomics 22, 71 (2021).","journal-title":"BMC Genomics"},{"key":"98159_CR54","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1038\/s41598-018-36550-6","volume":"9","author":"AK Tallon","year":"2019","unstructured":"Tallon, A. K., Hill, S. R. & Ignell, R. Sex and age modulate antennal chemosensory-related genes linked to the onset of host seeking in the yellow-fever mosquito Aedes aegypti. Sci. Rep. 9, 43 (2019).","journal-title":"Sci. Rep."},{"key":"98159_CR55","doi-asserted-by":"publisher","first-page":"9699","DOI":"10.1038\/s41598-019-46220-w","volume":"9","author":"AB Omondi","year":"2019","unstructured":"Omondi, A. B., Ghaninia, M., Dawit, M., Svensson, T. & Ignell, R. Age-dependent regulation of host seeking in Anopheles coluzzii. Sci. Rep. 9, 9699 (2019).","journal-title":"Sci. Rep."},{"key":"98159_CR56","doi-asserted-by":"publisher","first-page":"154085","DOI":"10.1016\/j.scitotenv.2022.154085","volume":"827","author":"RR Rix","year":"2022","unstructured":"Rix, R. R. & Cutler, G. C. Review of molecular and biochemical responses during stress induced stimulation and hormesis in insects. Sci. Total Environ. 827, 154085 (2022).","journal-title":"Sci. Total Environ."},{"key":"98159_CR57","doi-asserted-by":"publisher","first-page":"160467","DOI":"10.1098\/rsos.160467","volume":"3","author":"S Majeed","year":"2016","unstructured":"Majeed, S., Hill, S. R., Birgersson, G. & Ignell, R. Detection and perception of generic host volatiles by mosquitoes modulate host preference: context dependence of (R)-1-octen-3-ol. R. Soc. Open Sci. 3, 160467 (2016).","journal-title":"R. Soc. Open Sci."},{"key":"98159_CR58","doi-asserted-by":"publisher","DOI":"10.1101\/2023.08.01.548337","author":"Y Vainer","year":"2024","unstructured":"Vainer, Y. et al. A conserved odorant receptor underpins borneol-mediated repellency in culicine mosquitoes. Biorxiv https:\/\/doi.org\/10.1101\/2023.08.01.548337 (2024).","journal-title":"Biorxiv"},{"key":"98159_CR59","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s12983-020-0350-9","volume":"17","author":"ME Strader","year":"2020","unstructured":"Strader, M. E., Wong, J. M. & Hofmann, G. E. Ocean acidification promotes broad transcriptomic responses in marine metazoans: a literature survey. Front. Zool. 17, 7 (2020).","journal-title":"Front. Zool."},{"key":"98159_CR60","doi-asserted-by":"publisher","first-page":"e2527","DOI":"10.7717\/peerj.2527","volume":"4","author":"W Wu","year":"2016","unstructured":"Wu, W., Li, Z., Zhang, S., Ke, Y. & Hou, Y. Transcriptome response to elevated atmospheric CO2 concentration in the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). PeerJ 4, e2527 (2016).","journal-title":"PeerJ"},{"key":"98159_CR61","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1111\/gcb.14532","volume":"25","author":"CR Williams","year":"2019","unstructured":"Williams, C. R. et al. Elevated CO2 impairs olfactory-mediated neural and behavioral responses and gene expression in ocean-phase coho salmon (Oncorhynchus kisutch). Glob. Chang. Biol. 25, 963\u2013977 (2019).","journal-title":"Glob. Chang. Biol."},{"key":"98159_CR62","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1002\/ps.6630","volume":"78","author":"ZF Fan","year":"2022","unstructured":"Fan, Z. F. et al. Effects of elevated CO2 on activities of protective and detoxifying enzymes in Frankliniella occidentalis and F. intonsa under spinetoram stress. Pest Manag. Sci. 78, 274\u2013286 (2022).","journal-title":"Pest Manag. Sci."},{"key":"98159_CR63","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.3390\/insects13111029","volume":"13","author":"Z Lu","year":"2022","unstructured":"Lu, Z. et al. Effects of Elevated CO2 concentration on host adaptability and chlorantraniliprole susceptibility in Spodoptera frugiperda. Insects. 13, 1029 (2022).","journal-title":"Insects."},{"key":"98159_CR64","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.ibmb.2013.02.004","volume":"43","author":"MF L\u00f3pez","year":"2013","unstructured":"L\u00f3pez, M. F., Cano-Ram\u00edrez, C., Cesar-Ayala, A. K., Ruiz, E. A. & Z\u00fa\u00f1iga, G. Diversity and expression of P450 genes from Dendroctonus valens LeConte (Curculionidae: Scolytinae) in response to different kairomones. Insect Biochem. Mol. Biol. 43, 417\u2013432 (2013).","journal-title":"Insect Biochem. Mol. Biol."},{"key":"98159_CR65","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0293018","author":"F Mappin","year":"2023","unstructured":"Mappin, F., Bellantuono, A. J., Ebrahimi, B. & DeGennaro, M. Odor-evoked transcriptomics of Aedes aegypti mosquitoes. Biorxiv https:\/\/doi.org\/10.1371\/journal.pone.0293018 (2023).","journal-title":"Biorxiv"},{"key":"98159_CR66","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1007\/s00438-022-01890-6","volume":"297","author":"Y Zhang","year":"2022","unstructured":"Zhang, Y. et al. Response of xenobiotic biodegradation and metabolic genes in Tribolium castaneum following eugenol exposure. Mol. Genet. Genomics. 297, 801\u2013815 (2022).","journal-title":"Mol. Genet. Genomics."},{"key":"98159_CR67","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.jmb.2010.04.020","volume":"399","author":"WY Low","year":"2010","unstructured":"Low, W. Y. et al. Recognition and detoxification of the insecticide DDT by Drosophila melanogaster glutathione S-transferase D1. J. Mol. Biol. 399, 358\u2013366 (2010).","journal-title":"J. Mol. Biol."},{"key":"98159_CR68","doi-asserted-by":"publisher","first-page":"1568","DOI":"10.3389\/fphys.2019.01568","volume":"10","author":"H Li","year":"2019","unstructured":"Li, H. et al. Comparative transcriptome analysis of the heat stress response in Monochamus alternatus Hope (Coleoptera: Cerambycidae). Front. Physiol. 10, 1568 (2019).","journal-title":"Front. Physiol."},{"key":"98159_CR69","doi-asserted-by":"publisher","first-page":"4112","DOI":"10.1038\/s41467-023-39469-3","volume":"14","author":"Q Wang","year":"2023","unstructured":"Wang, Q. et al. Short-term particulate matter contamination severely compromises insect antennal olfactory perception. Nat. Commun. 14, 4112 (2023).","journal-title":"Nat. Commun."},{"key":"98159_CR70","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.jinsphys.2017.10.009","volume":"103","author":"N Li","year":"2017","unstructured":"Li, N., Li, Y., Zhang, S., Fan, Y. & Liu, T. Effect of elevated CO2 concentration and temperature on antioxidant capabilities of multiple generations of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae). J. Insect. Physiol. 103, 91\u201397 (2017).","journal-title":"J. Insect. Physiol."},{"key":"98159_CR71","doi-asserted-by":"crossref","unstructured":"Feyereisen, R. Insect CYP genes and P450 enzymes. In\u00a0Insect Molecular Biology and Biochemistry\u00a0(ed. Gilbert, L. I.) 236\u2013316 (Academic Press, 2012)","DOI":"10.1016\/B978-0-12-384747-8.10008-X"},{"key":"98159_CR72","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/j.cbpb.2005.01.019","volume":"140","author":"NH Badre","year":"2005","unstructured":"Badre, N. H., Martin, M. E. & Cooper, R. L. The physiological and behavioral effects of carbon dioxide on Drosophila melanogaster larvae. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 140, 363\u2013376 (2005).","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"98159_CR73","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1016\/j.jmb.2005.10.015","volume":"354","author":"mechanisms of ligand release","year":"2005","unstructured":"mechanisms of ligand release. Zubkov, S., Gronenborn, A. M., Byeon, In-Ja. L. & Mohanty, S. Structural consequences of the pH-induced conformational switch in A. polyphemus pheromone-binding protein. J. Mol. Biol. 354, 1081\u20131090 (2005).","journal-title":"J. Mol. Biol."},{"key":"98159_CR74","doi-asserted-by":"publisher","first-page":"125422","DOI":"10.1016\/j.ijbiomac.2023.125422","volume":"245","author":"B Mam","year":"2023","unstructured":"Mam, B. et al. Influence of pH on indole-dependent heterodimeric interactions between Anopheles gambiae odorant-binding proteins OBP1 and OBP4. Int. J. Biol. Macromol. 245, 125422 (2023).","journal-title":"Int. J. Biol. Macromol."},{"key":"98159_CR75","doi-asserted-by":"publisher","first-page":"1742","DOI":"10.1080\/07391102.2013.834118","volume":"32","author":"M Manoharan","year":"2013","unstructured":"Manoharan, M., Fuchs, P. F. J., Sowdhamini, R. & Offmann, B. Insights on pH-dependent conformational changes of mosquito odorant binding proteins by molecular dynamics simulations. J. Biomol. Struct. Dyn. 32, 1742\u20131751 (2013).","journal-title":"J. Biomol. Struct. Dyn."},{"key":"98159_CR76","doi-asserted-by":"crossref","unstructured":"Clark, T. M., Vieira, M. A. L., Huegel, K. L., Flury, D. & Carper, M. Strategies for regulation of hemolymph pH in acidic and alkaline water by the larval mosquito Aedes aegypti (L.) (Diptera; Culicidae). J. Exp. Biol. 210, 4359\u20134367 (2007).","DOI":"10.1242\/jeb.010694"},{"key":"98159_CR77","doi-asserted-by":"publisher","first-page":"2297","DOI":"10.1242\/jeb.01021","volume":"207","author":"TM Clark","year":"2004","unstructured":"Clark, T. M., Flis, B. J. & Remold, S. K. pH tolerances and regulatory abilities of freshwater and euryhaline Aedine mosquito larvae. J. Exp. Biol. 207, 2297\u20132304 (2004).","journal-title":"J. Exp. Biol."},{"key":"98159_CR78","doi-asserted-by":"publisher","first-page":"3893","DOI":"10.1038\/s41598-021-83024-3","volume":"11","author":"YV Bobkov","year":"2021","unstructured":"Bobkov, Y. V., Walker, W. B. III. & Cattaneo, A. M. Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3. Sci. Rep. 11, 3893 (2021).","journal-title":"Sci. Rep."},{"key":"98159_CR79","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1038\/s41558-018-0224-8","volume":"8","author":"CS Porteus","year":"2018","unstructured":"Porteus, C. S. et al. Near-future CO2 levels impair the olfactory system of a marine fish. Nat. Clim. Change. 8, 737\u2013743 (2018).","journal-title":"Nat. Clim. Change."},{"key":"98159_CR80","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.237941","author":"CS Porteus","year":"2021","unstructured":"Porteus, C. S., Roggatz, C. C., Velez, Z., Hardege, J. D. & Hubbard, P. C. Acidification can directly affect olfaction in marine organisms. J. Exp. Biol. https:\/\/doi.org\/10.1242\/jeb.237941 (2021).","journal-title":"J. Exp. Biol."},{"key":"98159_CR81","doi-asserted-by":"publisher","first-page":"509","DOI":"10.3390\/biom11040509","volume":"11","author":"K Rihani","year":"2021","unstructured":"Rihani, K., Ferveur, J. F. & Briand, L. The 40-Year mystery of insect odorant-binding proteins. Biomolecules 11, 509 (2021).","journal-title":"Biomolecules"},{"key":"98159_CR82","doi-asserted-by":"publisher","first-page":"1274197","DOI":"10.3389\/finsc.2023.1274197","volume":"3","author":"JA Abendroth","year":"2023","unstructured":"Abendroth, J. A., Moural, T. W., Wei, H. & Zhu, F. Roles of insect odorant-binding proteins in communication and xenobiotic adaptation. Front. Insect. Sci. 3, 1274197 (2023).","journal-title":"Front. Insect. Sci."},{"key":"98159_CR83","doi-asserted-by":"publisher","first-page":"3300","DOI":"10.1038\/s41598-020-60242-9","volume":"10","author":"J Wang","year":"2020","unstructured":"Wang, J., Murphy, E. J., Nix, J. C. & Jones, D. N. M. Aedes aegypti odorant-binding protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail. Sci. Rep. 10, 3300 (2020).","journal-title":"Sci. Rep."},{"key":"98159_CR84","doi-asserted-by":"crossref","unstructured":"Leal, G. M. & Leal, W. S. Binding of a fluorescence reporter and a ligand to an odorant-binding protein of the yellow fever mosquito, Aedes aegypti. F1000Res. 3, 305 (2014).","DOI":"10.12688\/f1000research.5879.1"},{"key":"98159_CR85","doi-asserted-by":"publisher","first-page":"e1005051","DOI":"10.1371\/journal.pgen.1005051","volume":"11","author":"S Jafari","year":"2015","unstructured":"Jafari, S. & Alenius, M. Cis-regulatory mechanisms for robust olfactory sensory neuron class-restricted odorant receptor gene expression in Drosophila. PLoS Genet. 11, e1005051 (2015).","journal-title":"PLoS Genet."},{"key":"98159_CR86","doi-asserted-by":"publisher","first-page":"103213","DOI":"10.1016\/j.ibmb.2019.103213","volume":"113","author":"F Zeng","year":"2019","unstructured":"Zeng, F., Xu, P. & Leal, W. S. Odorant receptors from Culex quinquefasciatus and Aedes aegypti sensitive to floral compounds. Insect Biochem. Mol. Biol. 113, 103213 (2019).","journal-title":"Insect Biochem. Mol. Biol."},{"key":"98159_CR87","doi-asserted-by":"crossref","unstructured":"Bernier, U. R., Kline, D. L., Barnard, D. R., Schreck, C. E. & Yost, R. A. Analysis of human skin emanations by gas chromatography\/mass spectrometry. 2. Identification of volatile compounds that are candidate attractants for the yellow fever mosquito (Aedes aegypti). Anal. Chem. 72, 747\u2013756 (1999).","DOI":"10.1021\/ac990963k"},{"key":"98159_CR88","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1093\/chemse\/bjq105","volume":"36","author":"JD Bohbot","year":"2011","unstructured":"Bohbot, J. D. et al. Conservation of indole responsive odorant receptors in mosquitoes reveals an ancient olfactory trait. Chem. Senses. 36, 149\u2013160 (2011).","journal-title":"Chem. Senses."},{"key":"98159_CR89","doi-asserted-by":"publisher","first-page":"533","DOI":"10.3389\/fncel.2018.00533","volume":"12","author":"DM Ruel","year":"2018","unstructured":"Ruel, D. M., Yakir, E. & Bohbot, J. D. Supersensitive odorant receptor underscores pleiotropic roles of indoles in mosquito ecology. Front. Cell. Neurosci. 12, 533 (2018).","journal-title":"Front. Cell. Neurosci."},{"key":"98159_CR90","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/s12864-015-2239-0","volume":"17","author":"BJ Matthews","year":"2016","unstructured":"Matthews, B. J., McBride, C. S., DeGennaro, M., Despo, O. & Vosshall, L. B. The neurotranscriptome of the Aedes aegypti mosquito. BMC Genomics 17, 32 (2016).","journal-title":"BMC Genomics"},{"key":"98159_CR91","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.ibmb.2014.02.007","volume":"48","author":"JD Bohbot","year":"2014","unstructured":"Bohbot, J. D., Sparks, J. T. & Dickens, J. C. The maxillary palp of Aedes aegypti, a model of multisensory integration. Insect Biochem. Mol. Biol. 48, 29\u201339 (2014).","journal-title":"Insect Biochem. Mol. Biol."},{"key":"98159_CR92","doi-asserted-by":"publisher","first-page":"112101","DOI":"10.1016\/j.celrep.2023.112101","volume":"42","author":"JI Raji","year":"2023","unstructured":"Raji, J. I., Konopka, J. K. & Potter, C. J. A spatial map of antennal-expressed ionotropic receptors in the malaria mosquito. Cell Rep. 42, 112101 (2023).","journal-title":"Cell Rep."},{"key":"98159_CR93","doi-asserted-by":"publisher","first-page":"759238","DOI":"10.3389\/fncel.2021.759238","volume":"15","author":"A Vulpe","year":"2021","unstructured":"Vulpe, A. & Menuz, K. Ir76b is a co-receptor for amine responses in Drosophila olfactory neurons. Front. Cell. Neurosci. 15, 759238 (2021).","journal-title":"Front. Cell. Neurosci."},{"key":"98159_CR94","doi-asserted-by":"publisher","first-page":"40297","DOI":"10.1038\/srep40297","volume":"7","author":"R Pitts","year":"2017","unstructured":"Pitts, R., Derryberry, S. L., Zhang, Z. & Zwiebel, L. J. Variant ionotropic receptors in the malaria vector mosquito Anopheles gambiae tuned to amines and carboxylic acids. Sci. Rep. 7, 40297 (2017).","journal-title":"Sci. Rep."},{"key":"98159_CR95","doi-asserted-by":"publisher","first-page":"9262","DOI":"10.1073\/pnas.0802505105","volume":"105","author":"L Ponnusamy","year":"2008","unstructured":"Ponnusamy, L. et al. Identification of bacteria and bacteria-associated chemical cues that mediate oviposition site preferences by Aedes aegypti. Proc. Natl. Acad. Sci. U.S.A. 105, 9262\u20139267 (2008).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"98159_CR96","doi-asserted-by":"crossref","unstructured":"Navarro-Silva, M. A., Marques, F. A. & Duque L, J. E. Review of semiochemicals that mediate the oviposition of mosquitoes: a possible sustainable tool for the control and monitoring of Culicidae. Rev. Bras. Entomol. 53, 1\u20136 (2009).","DOI":"10.1590\/S0085-56262009000100002"},{"key":"98159_CR97","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1038\/s41586-018-0732-8","volume":"564","author":"F van Breugel","year":"2018","unstructured":"van Breugel, F., Huda, A. & Dickinson, M. H. Distinct activity-gated pathways mediate attraction and aversion to CO2 in Drosophila. Nature 564, 420\u2013424 (2018).","journal-title":"Nature"},{"key":"98159_CR98","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1038\/nn.4100","volume":"18","author":"B von der Weid","year":"2015","unstructured":"von der Weid, B. et al. Large-scale transcriptional profiling of chemosensory neurons identifies receptor-ligand pairs in vivo. Nat. Neurosci. 18, 1455\u20131463 (2015).","journal-title":"Nat. Neurosci."},{"key":"98159_CR99","doi-asserted-by":"crossref","unstructured":"Taylor, B. & Jones, M. D. R. The circadian rhythm of flight activity in the mosquito Aedes aegypti (L.): the phase setting effects of light-on and light-off. J. Exp. Biol. 51, 59-70 (1968).","DOI":"10.1242\/jeb.51.1.59"},{"key":"98159_CR100","doi-asserted-by":"publisher","DOI":"10.1101\/016998","author":"DC Rinker","year":"2015","unstructured":"Rinker, D. C. et al. RNAseq in the mosquito maxillary palp: a little antennal RNA goes a long way. Biorxiv https:\/\/doi.org\/10.1101\/016998 (2015).","journal-title":"Biorxiv"},{"key":"98159_CR101","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","volume":"57","author":"Y Benjamini","year":"1995","unstructured":"Benjamini, Y. & Hochberg, Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Stat. Soc. B (Methodol.) 57, 289\u2013300 (1995).","journal-title":"J. R. Stat. Soc. B (Methodol.)"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98159-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98159-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98159-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T13:03:30Z","timestamp":1745499810000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-98159-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":101,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["98159"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-98159-w","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,24]]},"assertion":[{"value":"10 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"14318"}}