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Clinically, it is associated with urinary tract and other opportunistic infections. But reports on phages against them are scanty, which underlines the importance of the study. We isolated a\n                    <jats:italic>Staphylococcus saprophyticus<\/jats:italic>\n                    strain from soiled socks. The strain was characterised through biochemical tests and 16S rDNA ribotyping. Furthermore, the antibiogram revealed that the strain is resistant to penicillinase-resistant beta-lactam antibiotics. A double-stranded DNA temperate phage, \u00d8Ph_SS01, was isolated against\n                    <jats:italic>S. saprophyticus<\/jats:italic>\n                    from a mixed sewage sample. The phage is stable over a wide range of temperatures, including pH and salt, with 67.08\u2009\u00b1\u200925 virions\/cell following adsorption within 14\u00a0min. The potential of \u00d8Ph_SS01 was established in Mannitol Salt broth with a 7-log reduction of\n                    <jats:italic>S. saprophyticus<\/jats:italic>\n                    over a period of 24\u00a0h. Moreover, it inhibited and disrupted biofilm at efficiencies of 77% and 69%, respectively, at an MOI of 100. Studies on adherent immortalised human keratinocytes and urethral cell lines did not result in any significant change in viability. Sequence analysis of \u00d8Ph_SS01 depicted a genome size of 47 Kb with 37.36% GC content. A similarity of 91.5% to related entries indicated its novelty. The textile matrices immobilised with phages could reduce the bacterial load by about 7-log, which shows the potential of the phage in infection control.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-35899-3","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:35:37Z","timestamp":1770330937000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation, characterisation and potential applications of a novel bacteriophage targeting beta-lactam-resistant Staphylococcus saprophyticus"],"prefix":"10.1038","volume":"16","author":[{"given":"O.","family":"Gopika","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niti","family":"Sarat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maanya","family":"Manikandan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sumana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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