{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T13:13:05Z","timestamp":1771593185047,"version":"3.50.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":16,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-74-30011"],"award-info":[{"award-number":["19-74-30011"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-74-30011"],"award-info":[{"award-number":["19-74-30011"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-74-30011"],"award-info":[{"award-number":["19-74-30011"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-74-30011"],"award-info":[{"award-number":["19-74-30011"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-74-30011"],"award-info":[{"award-number":["19-74-30011"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-74-30011"],"award-info":[{"award-number":["19-74-30011"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-74-30011"],"award-info":[{"award-number":["19-74-30011"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002674","name":"Russian Academy of Sciences","doi-asserted-by":"publisher","award":["125012300659-6"],"award-info":[{"award-number":["125012300659-6"]}],"id":[{"id":"10.13039\/501100002674","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002674","name":"Russian Academy of Sciences","doi-asserted-by":"publisher","award":["125012300659-6"],"award-info":[{"award-number":["125012300659-6"]}],"id":[{"id":"10.13039\/501100002674","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-36260-4","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T14:53:29Z","timestamp":1770216809000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Two paired HPV-59 cervical cancer cell lines with distinct chemoradioresistant phenotypes"],"prefix":"10.1038","volume":"16","author":[{"given":"Irina","family":"Tatarnikova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vadim","family":"Talyshev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Sen\u2019kova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oleg","family":"Markov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darya","family":"Mikhailova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana","family":"Pashkovskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Krasilnikov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Samoilova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana","family":"Gainer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgeniy","family":"Brener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Zenkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgeniya","family":"Logashenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"36260_CR1","first-page":"229","volume":"74","author":"F Bray","year":"2024","unstructured":"Bray, F. et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 74, 229\u2013263 (2024).","journal-title":"CA Cancer J. Clin."},{"key":"36260_CR2","doi-asserted-by":"crossref","unstructured":"Yang, M. et al. Global trends and age-specific incidence and mortality of cervical cancer from 1990 to 2019: an international comparative study based on the global burden of disease. BMJ Open 12, (2022).","DOI":"10.1136\/bmjopen-2021-055470"},{"key":"36260_CR3","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1016\/j.ygyno.2021.10.075","volume":"163","author":"WQ He","year":"2021","unstructured":"He, W. Q. & Li, C. Recent global burden of cervical cancer incidence and mortality, predictors, and Temporal trends. Gynecol. Oncol. 163, 583\u2013592 (2021).","journal-title":"Gynecol. Oncol."},{"key":"36260_CR4","doi-asserted-by":"publisher","unstructured":"Liu, Y. & Zheng, W. Cervical cancer Development, Screening, and prevention. Gynecol. Obstet. Pathol. 1\u201316 https:\/\/doi.org\/10.1007\/978-981-19-7696-4_52-1 (2024).","DOI":"10.1007\/978-981-19-7696-4_52-1"},{"key":"36260_CR5","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.1007\/s00432-021-03738-9","volume":"147","author":"F Petrelli","year":"2021","unstructured":"Petrelli, F. et al. Human papillomavirus (HPV) types 16 and 18 infection and esophageal squamous cell carcinoma: a systematic review and meta-analysis. J. Cancer Res. Clin. Oncol. 147, 3011\u20133023 (2021).","journal-title":"J. Cancer Res. Clin. Oncol."},{"key":"36260_CR6","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1038\/s41571-024-00904-z","volume":"21","author":"T Malag\u00f3n","year":"2024","unstructured":"Malag\u00f3n, T., Franco, E. L., Tejada, R. & Vaccarella, S. Epidemiology of HPV-associated cancers past, present and future: towards prevention and elimination. Nat. Rev. Clin. Oncol. 21, 522\u2013538 (2024).","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"36260_CR7","doi-asserted-by":"publisher","first-page":"720","DOI":"10.21147\/j.issn.1000-9604.2020.06.05","volume":"32","author":"S Zhang","year":"2020","unstructured":"Zhang, S., Xu, H., Zhang, L. & Qiao, Y. Cervical cancer: Epidemiology, risk factors and screening. Chin. J. Cancer Res. 32, 720\u2013728 (2020).","journal-title":"Chin. J. Cancer Res."},{"key":"36260_CR8","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.ygyno.2022.08.013","volume":"167","author":"E Pujade-Lauraine","year":"2022","unstructured":"Pujade-Lauraine, E. et al. Comparison of global treatment guidelines for locally advanced cervical cancer to optimize best care practices: A systematic and scoping review. Gynecol. Oncol. 167, 360\u2013372 (2022).","journal-title":"Gynecol. Oncol."},{"key":"36260_CR9","doi-asserted-by":"publisher","first-page":"4458","DOI":"10.3390\/jcm13154458","volume":"13","author":"R. Massobrio","year":"2024","unstructured":"Massobrio, R. et al. New frontiers in locally advanced cervical cancer treatment. J. Clin. Med. 13, 4458 (2024).","journal-title":"J. Clin. Med."},{"key":"36260_CR10","doi-asserted-by":"publisher","first-page":"100832","DOI":"10.1016\/j.adro.2021.100832","volume":"7","author":"MA Noy","year":"2021","unstructured":"Noy, M. A. et al. Levels of evidence for radiation therapy recommendations in the National comprehensive cancer network (NCCN) clinical guidelines. Adv. Radiat. Oncol. 7, 100832 (2021).","journal-title":"Adv. Radiat. Oncol."},{"key":"36260_CR11","first-page":"17","volume":"73","author":"RL Siegel","year":"2023","unstructured":"Siegel, R. L., Miller, K. D., Wagle, N. S. & Jemal, A. Cancer statistics, 2023. CA Cancer J. Clin. 73, 17\u201348 (2023).","journal-title":"CA Cancer J. Clin."},{"key":"36260_CR12","unstructured":"Capes-Davis, A. Database of Cross-Contaminated or misidentified cell lines international cell line authentication committee (ICLAC). (2013)."},{"key":"36260_CR13","doi-asserted-by":"publisher","first-page":"1885","DOI":"10.1038\/s41416-024-02675-x","volume":"130","author":"A Makowska","year":"2024","unstructured":"Makowska, A., Kontny, U. & Weiskirchen, R. HeLa cells cross-contaminated nasopharyngeal carcinoma cell lines: still a common problem. Br. J. Cancer. 130, 1885\u20131886 (2024).","journal-title":"Br. J. Cancer"},{"key":"36260_CR14","doi-asserted-by":"publisher","first-page":"1404","DOI":"10.1016\/j.juro.2013.03.009","volume":"190","author":"W J\u00e4ger","year":"2013","unstructured":"J\u00e4ger, W. et al. Hiding in plain view: genetic profiling reveals decades old cross contamination of bladder cancer cell line KU7 with HeLa. J. Urol. 190, 1404\u20131409 (2013).","journal-title":"J. Urol."},{"key":"36260_CR15","doi-asserted-by":"publisher","first-page":"5167","DOI":"10.3390\/ijms23095167","volume":"23","author":"R. Di Fiore","year":"2022","unstructured":"Di Fiore, R. et al. Cancer stem cells and their possible implications in cervical cancer: A short review. Int. J. Mol. Sci. 23, 5167 (2022).","journal-title":"Int. J. Mol. Sci."},{"key":"36260_CR16","doi-asserted-by":"publisher","first-page":"3790","DOI":"10.3390\/cells11233790","volume":"11","author":"G. M. Wen","year":"2022","unstructured":"Wen, G. M., Xu, X. Y. & Xia, P. Metabolism in Cancer Stem Cells: Targets for Clinical Treatment. Cells 11, 3790 (2022).","journal-title":"Cells"},{"key":"36260_CR17","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.3390\/cancers6031570","volume":"6","author":"M Ishikawa","year":"2014","unstructured":"Ishikawa, M. et al. Simultaneous expression of cancer stem cell-like properties and cancer-associated fibroblast-like properties in a primary culture of breast cancer cells. Cancers (Basel). 6, 1570\u20131578 (2014).","journal-title":"Cancers (Basel)"},{"key":"36260_CR18","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1136\/ijgc-2019-000942","volume":"30","author":"K Tucker","year":"2020","unstructured":"Tucker, K. et al. Defining the learning curve for successful staging with Sentinel lymph node biopsy for endometrial cancer among surgeons at an academic institution. Int. J. Gynecol. Cancer. 30, 346\u2013351 (2020).","journal-title":"Int. J. Gynecol. Cancer"},{"key":"36260_CR19","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1515\/med-2019-0036","volume":"14","author":"Y Liu","year":"2019","unstructured":"Liu, Y., Cao, C., Zhai, P. & Zhang, Y. Biological characteristics of cervical precancerous cell proliferation. Open. Med. (Warsaw Poland). 14, 362\u2013368 (2019).","journal-title":"Open. Med. (Warsaw Poland)"},{"key":"36260_CR20","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.canlet.2009.05.034","volume":"287","author":"F Darroudi","year":"2010","unstructured":"Darroudi, F. et al. PCC and COBRA-FISH a new tool to characterize primary cervical carcinomas: to assess hall-marks and stage specificity. Cancer Lett. 287, 67\u201374 (2010).","journal-title":"Cancer Lett."},{"key":"36260_CR21","doi-asserted-by":"publisher","first-page":"7683","DOI":"10.1002\/jcp.28049","volume":"234","author":"H Deng","year":"2019","unstructured":"Deng, H., Mondal, S., Sur, S. & Woodworth, C. D. Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. J. Cell. Physiol. 234, 7683\u20137694 (2019).","journal-title":"J. Cell. Physiol."},{"key":"36260_CR22","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9","volume":"182","author":"T Scholzen","year":"2000","unstructured":"Scholzen, T. & Gerdes, J. The Ki-67 protein: from the known and the unknown. J. Cell. Physiol. 182, 311\u2013322 (2000).","journal-title":"J. Cell. Physiol."},{"key":"36260_CR23","first-page":"465","volume":"2018","author":"P Kumar","year":"2018","unstructured":"Kumar, P., Nagarajan, A. & Uchil, P. D Analysis of cell viability by the lactate dehydrogenase assay. Cold Spring Harb Protoc. 2018, 465\u2013468 (2018).","journal-title":"Cold Spring Harb Protoc."},{"key":"36260_CR24","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1080\/09553002.2018.1490041","volume":"95","author":"MI Koukourakis","year":"2019","unstructured":"Koukourakis, M. I. & Giatromanolaki, A. Warburg effect, lactate dehydrogenase, and radio\/chemo-therapy efficacy. Int. J. Radiat. Biol. 95, 408\u2013426 (2019).","journal-title":"Int. J. Radiat. Biol."},{"key":"36260_CR25","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1385\/MB:32:3:227","volume":"32","author":"GR Stark","year":"2006","unstructured":"Stark, G. R. & Taylor, W. R. Control of the G2\/M transition. Mol. Biotechnol. 32, 227\u2013248 (2006).","journal-title":"Mol. Biotechnol."},{"key":"36260_CR26","doi-asserted-by":"publisher","first-page":"4689","DOI":"10.1038\/sj.onc.1210268","volume":"26","author":"Y Yan","year":"2007","unstructured":"Yan, Y., Black, C. P. & Cowan, K. H. Irradiation-induced G2\/M checkpoint response requires ERK1\/2 activation. Oncogene 26, 4689\u20134698 (2007).","journal-title":"Oncogene"},{"key":"36260_CR27","doi-asserted-by":"publisher","first-page":"26880","DOI":"10.3390\/ijms161125991","volume":"16","author":"BM Kim","year":"2015","unstructured":"Kim, B. M. et al. Therapeutic implications for overcoming radiation resistance in cancer therapy. Int. J. Mol. Sci. 16, 26880\u201326913 (2015).","journal-title":"Int. J. Mol. Sci."},{"key":"36260_CR28","doi-asserted-by":"publisher","first-page":"9369","DOI":"10.3390\/ijms22179369","volume":"22","author":"T. Tanaka","year":"2021","unstructured":"Tanaka, T. et al. Patient-Derived xenograft models in cervical cancer: A systematic review. Int. J. Mol. Sci. 22, 9369 (2021).","journal-title":"Int. J. Mol. Sci."},{"key":"36260_CR29","doi-asserted-by":"publisher","first-page":"1380","DOI":"10.1016\/j.stem.2021.03.012","volume":"28","author":"K L\u00f5hmussaar","year":"2021","unstructured":"L\u00f5hmussaar, K. et al. Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer. Cell. Stem Cell. 28, 1380\u20131396e6 (2021).","journal-title":"Cell. Stem Cell."},{"key":"36260_CR30","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1080\/01443615.2019.1634030","volume":"40","author":"K. S. Okunade","year":"2020","unstructured":"Okunade, K. S. Human papillomavirus and cervical cancer. J. Obstet. Gynaecol. 40 602\u2013608 at (2020). https:\/\/doi.org\/10.1080\/01443615.2019.1634030","journal-title":"J. Obstet. Gynaecol."},{"key":"36260_CR31","first-page":"151","volume":"17","author":"I Ishiwata","year":"2004","unstructured":"Ishiwata, I. et al. Establishment and characterization of human uterine cervical epidermoid carcinoma cell line HHUS containing HPV 59 DNA. Hum. cell. Off J. Hum. Cell. Res. Soc. 17, 151\u2013156 (2004).","journal-title":"Hum. cell. Off J. Hum. Cell. Res. Soc."},{"issue":"5","key":"36260_CR32","first-page":"1","volume":"51","author":"A Frattini","year":"2015","unstructured":"Frattini, A. et al. High variability of genomic instability and gene expression profiling in different HeLa clones. Sci. Rep. 2015. 51 (5), 1\u20139 (2015).","journal-title":"Sci. Rep. 2015"},{"key":"36260_CR33","doi-asserted-by":"publisher","first-page":"449","DOI":"10.2353\/jmoldx.2006.060033","volume":"8","author":"G Ng","year":"2006","unstructured":"Ng, G., Huang, J., Roberts, I. & Coleman, N. Defining Ploidy-Specific thresholds in array comparative genomic hybridization to improve the sensitivity of detection of single copy alterations in cell lines. J. Mol. Diagn. 8, 449 (2006).","journal-title":"J. Mol. Diagn."},{"key":"36260_CR34","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1038\/nature12624","volume":"501","author":"CE Meacham","year":"2013","unstructured":"Meacham, C. E. & Morrison, S. J. Tumor heterogeneity and cancer cell plasticity. Nature 501, 328 (2013).","journal-title":"Nature"},{"key":"36260_CR35","unstructured":"The Genetics of Cancer - NCI. https:\/\/www.cancer.gov\/about-cancer\/causes-prevention\/genetics"},{"key":"36260_CR36","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1007\/s10585-023-10260-4","volume":"41","author":"JL Xing","year":"2024","unstructured":"Xing, J. L. & Stea, B. Molecular mechanisms of sensitivity and resistance to radiotherapy. Clin. Exp. Metastasis. 41, 517\u2013524 (2024).","journal-title":"Clin. Exp. Metastasis"},{"key":"36260_CR37","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1080\/09553002.2022.2047825","volume":"98","author":"JC Alamilla-Presuel","year":"2022","unstructured":"Alamilla-Presuel, J. C., Burgos-Molina, A. M., Gonz\u00e1lez-Vidal, A., Sendra-Portero, F. & Ruiz-G\u00f3mez, M. J. Factors and molecular mechanisms of radiation resistance in cancer cells. Int. J. Radiat. Biol. 98, 1301\u20131315 (2022).","journal-title":"Int. J. Radiat. Biol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-36260-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-36260-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-36260-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:34:29Z","timestamp":1771590869000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-36260-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,4]]},"references-count":37,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["36260"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-36260-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,4]]},"assertion":[{"value":"2 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The study was approved by the Ethics Committee of the Institute of Chemical Biology and Fundamental Medicine (No 1 from January 11 2022), with the patient\u2019s informed consent, and conducted according to the Declaration of Helsinki. All animal procedures were carried out in strict accordance with the European Communities Council Directive 86\/609\/CEE. The experimental protocol was approved by the Committee on the Ethics of Animal Experiments at the Institute of Cytology and Genetics SB RAS (protocol no. 111 of 7 December 2021). This study was also conducted in accordance with the guidelines of the Animal Research: Reporting of In Vivo Experiments (ARRIVE).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}],"article-number":"7307"}}