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The current study focuses on the molecular genotyping of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    recovered from human, water, and food specimens. A total of 350 samples, fifty samples each from human, tap water, fish swamp, chicken meat, minced meat, raw milk, and hospital surface from the Menoufiya governorate, Egypt.\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    was detected in 14.28%, including human (26%), tap water (18%), fish swamp (18%), chicken meat (12%), minced meat (10%), raw milk (16%), and hospital surface (0%). The results of testing of 50\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    isolates against sixteen antibiotics revealed a relatively high antibiotic resistance for Amoxicillin (100%), Erythromycin (98%), Cephradine (90%), Colistin (82%), Oxytetracyclin, (79%), Chloramphenicol (70%), Doxycycline (70%), and Kanamycin (62%) and high susceptibility for Imipenem (96%), Apramycin (94%), Amikacin (90%), Norfloxacin (78%), Sulphamethoxazol (86%), Enrofloxacin (64%), and Ofloxacin (60%). Furthermore, PCR was successfully amplified for the\n                    <jats:italic>tox<\/jats:italic>\n                    A,\n                    <jats:italic>exo<\/jats:italic>\n                    S, and\n                    <jats:italic>opr<\/jats:italic>\n                    L virulence genes at 396, 118, and 504\u00a0bp, respectively, as well as amplifying the\n                    <jats:italic>erm<\/jats:italic>\n                    B,\n                    <jats:italic>pel<\/jats:italic>\n                    A,\n                    <jats:italic>bla<\/jats:italic>\n                    TEM, and\n                    <jats:italic>tet<\/jats:italic>\n                    A resistance genes at 639, 786, 516, and 570\u00a0bp, respectively. The dendrogram investigation by ERIC-PCR of 10 clinical\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    isolates revealed two main clusters and 10 different ERIC-PCR patterns. The presence of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    isolates in food may represent a potential public health concern, with the need for further epidemiological studies, as well as whole-genome sequencing and correlations of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    in water, food samples, and human infections.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-37559-y","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T13:04:02Z","timestamp":1771333442000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prevalence and genotyping of Pseudomonas aeruginosa from food and human sources"],"prefix":"10.1038","volume":"16","author":[{"given":"Walid S.","family":"Mousa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman E.","family":"Abdeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanem F.","family":"El-Gendy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem M.","family":"Alsaadawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary M.","family":"Hana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"37559_CR1","doi-asserted-by":"publisher","first-page":"199","DOI":"10.3390\/cells12010199","volume":"12","author":"SJ Wood","year":"2023","unstructured":"Wood, S. 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